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Gene information
General information
TC009452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1002800..1006184
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009452
    iBeetle screen iB_04634

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009452 RA
      MKLLKLFLV VFLQIYAKA TPSVLIVGS GPAGIAAAT 
      RLLEHNHKN IRVLEAENR IGGRINSVF FGQAFVDLG 
      AESCHGQKG NVVYDMVKN LGVLKHVDG PRSVYHSSR 
      KEIVFGDEL LRIIDGIYG PDGQRDEDE GKSVGHYCL 
      DKYNASIYD KYRNDAEKF EIAKASVDL FHHIVLSYE 
      GAFSWFEPS AKSDYRDCE GDLSLNWNG LGYKTVLEV 
      MMKKFPNPS EQLPFDETV LLNKEVVKV FWNDSSSHN 
      AVTVYCSDH SSYTADHVI FTPSIGVLK ERHETMFTP 
      QLSEAKKDA IKHIGFGAV MKIAMFFKH RWWESERNF 
      TGFHFVWSE GDKSRAFKE FPEGPLKDG HSWLTEFFC 
      VVPVDRNPN VLVGWLTGS MVPEIELMT NETLIDGLE 
      FVLNKFLGH KYNITGPDS IIRTYWHTN PHFRGSYSY 
      QTVEARKDK ITAEMELAK PVLNLEGRP ILQFAGEAS 
      HPYFYSTVH GAIETGFRE ADRIINSYK
    CDS sequence
    • >TC009452 RA
      atgaagctc ctcaagctt tttctcgtc gtatttttg 
      caaatttac gccaaagcg acgccctcc gtgctaatc 
      gtgggctct ggacctgct ggcatcgca gcagcgacg 
      agactcctc gaacacaac cacaagaac attcgagta 
      ttagaggca gaaaaccga atcgggggc cgaattaac 
      agcgttttt ttcggccaa gctttcgtc gatttgggc 
      gcagagtcg tgccatggg cagaaaggt aacgtggtc 
      tatgacatg gtcaaaaat ttgggcgtt ttgaaacat 
      gtggatggg ccacggagt gtctaccac tcctcacgc 
      aaagaaatc gtttttggg gacgagttg ttgcgaatt 
      attgatggg atttacggc cctgatggg caaagggac 
      gaagatgag ggcaagagt gtggggcat tactgcttg 
      gacaaatac aacgccagc atttacgat aagtaccgg 
      aacgatgcg gagaaattt gaaattgcg aaagcgtcc 
      gttgatctc ttccatcac atagtatta agctacgag 
      ggcgccttt tcgtggttc gaaccctcg gccaagagc 
      gactaccgc gattgcgag ggcgacctc agcctgaac 
      tggaacggg ctgggctac aagaccgtg ctggaagtc 
      atgatgaag aagtttcca aacccgtcc gaacaactc 
      ccgtttgac gaaacagta ttactaaac aaggaagta 
      gttaaagta ttttggaac gacagttct agtcataat 
      gcggtgacg gtctactgc tcggatcat tcgagttac 
      acggccgat catgtcatt ttcactcct tcgattgga 
      gtgttgaag gagcggcac gaaacaatg tttacgcca 
      cagttatcg gaggcaaaa aaggacgcg atcaaacac 
      atcggtttt ggggctgtt atgaaaatt gcgatgttt 
      ttcaaacac cggtggtgg gaaagcgag aggaatttt 
      acaggtttt cactttgtt tggagcgag ggggacaaa 
      agtcgcgct tttaaggag tttccagaa gggccacta 
      aaggacggc cactcctgg ctcactgaa tttttttgc 
      gtcgtccct gtagatcgc aaccctaat gtgctagtt 
      ggttggttg acgggttca atggttccc gaaattgag 
      ctaatgacc aacgaaaca ctaatcgat ggtttggag 
      tttgttctc aataaattt ctagggcat aaatataac 
      ataaccggg cctgattcc ataattcgt acttattgg 
      cacacaaac ccgcatttt cggggcagt tactcttat 
      cagaccgtc gaagctcgc aaagataaa atcacagct 
      gagatggag ttggctaag cctgtgcta aatctggaa 
      ggacggccc attttacag tttgcagga gaggcctca 
      catccctat ttttactca actgtgcat ggggccatc 
      gagactgga tttagagaa gcggacaga attattaat 
      tcttacaaa tag
    mRNA sequence
    • >TC009452 RA
      cataatctc acaacacag ctgtgaaag ttcggtcaa 
      tgaaaatga agctcctca agctttttc tcgtcgtat 
      ttttgcaaa tttacgcca aagcgacgc cctccgtgc 
      taatcgtgg gctctggac ctgctggca tcgcagcag 
      cgacgagac tcctcgaac acaaccaca agaacattc 
      gagtattag aggcagaaa accgaatcg ggggccgaa 
      ttaacagcg tttttttcg gccaagctt tcgtcgatt 
      tgggcgcag agtcgtgcc atgggcaga aaggtaacg 
      tggtctatg acatggtca aaaatttgg gcgttttga 
      aacatgtgg atgggccac ggagtgtct accactcct 
      cacgcaaag aaatcgttt ttggggacg agttgttgc 
      gaattattg atgggattt acggccctg atgggcaaa 
      gggacgaag atgagggca agagtgtgg ggcattact 
      gcttggaca aatacaacg ccagcattt acgataagt 
      accggaacg atgcggaga aatttgaaa ttgcgaaag 
      cgtccgttg atctcttcc atcacatag tattaagct 
      acgagggcg ccttttcgt ggttcgaac cctcggcca 
      agagcgact accgcgatt gcgagggcg acctcagcc 
      tgaactgga acgggctgg gctacaaga ccgtgctgg 
      aagtcatga tgaagaagt ttccaaacc cgtccgaac 
      aactcccgt ttgacgaaa cagtattac taaacaagg 
      aagtagtta aagtatttt ggaacgaca gttctagtc 
      ataatgcgg tgacggtct actgctcgg atcattcga 
      gttacacgg ccgatcatg tcattttca ctccttcga 
      ttggagtgt tgaaggagc ggcacgaaa caatgttta 
      cgccacagt tatcggagg caaaaaagg acgcgatca 
      aacacatcg gttttgggg ctgttatga aaattgcga 
      tgtttttca aacaccggt ggtgggaaa gcgagagga 
      attttacag gttttcact ttgtttgga gcgaggggg 
      acaaaagtc gcgctttta aggagtttc cagaagggc 
      cactaaagg acggccact cctggctca ctgaatttt 
      tttgcgtcg tccctgtag atcgcaacc ctaatgtgc 
      tagttggtt ggttgacgg gttcaatgg ttcccgaaa 
      ttgagctaa tgaccaacg aaacactaa tcgatggtt 
      tggagtttg ttctcaata aatttctag ggcataaat 
      ataacataa ccgggcctg attccataa ttcgtactt 
      attggcaca caaacccgc attttcggg gcagttact 
      cttatcaga ccgtcgaag ctcgcaaag ataaaatca 
      cagctgaga tggagttgg ctaagcctg tgctaaatc 
      tggaaggac ggcccattt tacagtttg caggagagg 
      cctcacatc cctattttt actcaactg tgcatgggg 
      ccatcgaga ctggattta gagaagcgg acagaatta 
      ttaattctt acaaatagt tatttattg cactgtttt 
      gtaatgtaa ataagacat tttttgtat ttgtatttg 
      tgtttgtcg gaaggaaat aaacgtaaa atacgata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY