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Gene information
General information
TC009435 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1296920..1300614
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009435
    iBeetle screen iB_04632

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009435 RA
      MSGQWEVVG KKKEKGAKD LSQKVGKQS RNKPENLNV 
      KVEDVLAKS QVKNLYSNG KNKENKPVE VKKDAGPKK 
      AKVQDKVQE AKPKAPKSL ESALNLIDS DAFKNIFEK 
      NKTQFPDAP IVHDSVFAS KPDNYPLKI VPSGVLSVI 
      EKVAQVVDK NNLLVFYDI ALTSMATDM SKNLPALGY 
      KLFLQHIAL KEPKLIRAN LGKYCVLRT SYQNRPNIG 
      LSILWAVGQ AGIRDFQTG LAIFQELML PLIELKNYS 
      RFIVQYLIK LISGADQAT VSKEQFVSL LDVVFSSKK 
      NFPSDLKQD LVTVVPTLK SFLATSKEP TCVDFYLKK 
      IPTVGNDSF KDCLCDVIV DGFNRDQSS LESWGKNYS 
      KNVTSSAIL LKYIAKNWQ TLSCKINKK VLAHTLNQI 
      LLESNELRT RKKKDEGLI EAIRVSEEL LGKMANKKS 
      SRSWFKFLF YTTTLLLAI YIYADTKQA GSWQKSNTR 
      KFLVETGVY DYTHKAVGK VQEGWLVVD NKIKENFPT 
      YRQAVIEFS EPYIEFFNS FGQILCNLF ANIKEAVIE 
      KYPVVVKSI DSYAPGVVE QSQNAVSTA WSSSVFYVN 
      RSIDYLRTE VFVGQLSPE NMQRVVYEA FNTTQTKAT 
      EYYHWLYEK VQNTIK
    • >TC009435 RB
      MSGQWEVVG KKKEKGAKD LSQKVGKQS RNKPENLNV 
      KVEDVLAKS QVKNLYSNG KNKENKPVE VKKDAGPKK 
      AKVQDKVQE AKPKAPKSL ESALNLIDS DAFKNIFEK 
      NKTQFPDAP IVHDSVFAS KPDNYPLKI VPSGVLSVI 
      EKVAQVVDK NNLLVFYDI ALTSMATDM SKNLPALGY 
      KLFLQHIAL KEPKLIRAN LGKYCVLRT SYQNRPNIG 
      LSILWAVGQ AGIRDFQTG LAIFQELML PLIELKNYS 
      RFIVQYLIK LISGADQAT VSKEQFVSL LDVVFSSKK 
      NFPSDLKQD LVTVVPTLK SFLATSKEP TCVDFYLKK 
      IPTVGNDSF KDCLCDVIV DGFNRDQSS LESWGKNYT 
      KNWQTLSCK INKKVLAHT LNQILLESN ELRTRKKKD 
      EGLIEAIRV SEELLGKMA NKKSSRSWF KFLFYTTTL 
      LLAIYIYAD TKQAGSWQK SNTRKFLVE TGVYDYTHK 
      AVGKVQEGW LVVDNKIKE NFPTYRQAV IEFSEPYIE 
      FFNSFGQIL CNLFANIKE AVIEKYPVV VKSIDSYAP 
      GVVEQSQNA VSTAWSSSV FYVNRSIDY LRTEVFVGQ 
      LSPENMQRV VYEAFNTTQ TKATEYYHW LYEKVQNTI 
      K
    CDS sequence
    • >TC009435 RA
      atgtcgggt caatgggaa gttgttggc aaaaaaaag 
      gaaaaggga gccaaggat ttaagtcaa aaagttggc 
      aaacaaagt agaaacaaa ccggaaaat ttgaatgta 
      aaagttgag gatgttttg gcgaaatcc caagtcaaa 
      aacctgtat tccaacgga aaaaataaa gaaaacaaa 
      ccggtggaa gtaaaaaaa gatgcgggc cccaaaaaa 
      gcgaaagtt caagataag gtgcaggag gccaagcct 
      aaggcgccc aaatcgctc gaaagtgca cttaatttg 
      attgacagt gatgcgttc aaaaacatc tttgagaaa 
      aacaagacg caatttccc gacgcccct atcgtacat 
      gattccgtt tttgcctca aaacccgat aactatccg 
      ttgaaaatt gtgccctct ggagttcta tctgtgatt 
      gagaaagtg gcccaagtt gtggataaa aacaacctt 
      ttggtgttt tacgacatt gccttaaca tcaatggcc 
      actgatatg agcaaaaat ttgccagcg ttaggttac 
      aagttgttt ctgcaacac atcgcctta aaggaacct 
      aaattaatt cgtgccaat ttggggaaa tattgtgtc 
      ttgcgaacg tcttatcag aaccgacca aacatcgga 
      ttatcaatt ttatgggct gtaggacag gctgggatt 
      agggatttt cagacggga ttagctatt tttcaggag 
      ttaatgtta ccgttaatt gaattgaaa aattattca 
      cgatttatc gtccagtat ttgattaag ttaatttcg 
      ggggctgac caagcgaca gttagtaaa gaacagttc 
      gtttcactt cttgatgtt gtgttttcg agcaagaaa 
      aactttccc agtgatctt aaacaagat ctcgtgaca 
      gttgtacca actttgaag tcgttccta gcgaccagt 
      aaagagcca acttgtgtc gacttttat ttgaagaaa 
      ataccaact gttggaaac gactcgttt aaagactgt 
      ttatgtgac gttatcgtt gatggtttt aatcgagat 
      cagagcagt ttggagagt tggggaaaa aattatagt 
      aagaacgtc acctcaagt gccattctt cttaaatat 
      attgcgaaa aattggcaa actctttct tgtaaaata 
      aataagaaa gtgttggcg catacactt aaccaaatt 
      cttctcgaa agtaacgaa ttgaggact aggaaaaag 
      aaagacgaa ggtttgatt gaagccatt cgtgtatca 
      gaggaatta ttggggaaa atggccaat aagaaaagc 
      tcccgttcg tggttcaaa ttcctcttt tacacaacg 
      actttattg ctcgcaatt tacatttat gctgacacc 
      aaacaagcc ggcagttgg caaaaatcc aacacaagg 
      aaattcctg gtagagaca ggtgtgtat gactacacg 
      cataaagct gtgggtaaa gtccaagaa ggttggctg 
      gtagtggac aacaaaatc aaggaaaat tttcctact 
      tatcgccaa gcggttatt gagttctcg gaaccttat 
      attgagttt tttaatagt tttggacaa attctctgc 
      aatttgttc gccaatatc aaggaggct gtcatcgag 
      aagtaccca gtggtcgtt aaatcgatt gactcatat 
      gctcctggc gttgtggag cagtcccag aacgcagtg 
      agcacagcg tggtcaagt tctgtattt tatgtcaac 
      agaagcatt gattattta cgtacggaa gtttttgtc 
      ggccagtta tctcctgaa aatatgcaa agggtggtg 
      tatgaggct ttcaacacc actcagacc aaggcgact 
      gaatattac cactggttg tatgaaaag gtccaaaat 
      acgatcaaa taa
    • >TC009435 RB
      atgtcgggt caatgggaa gttgttggc aaaaaaaag 
      gaaaaggga gccaaggat ttaagtcaa aaagttggc 
      aaacaaagt agaaacaaa ccggaaaat ttgaatgta 
      aaagttgag gatgttttg gcgaaatcc caagtcaaa 
      aacctgtat tccaacgga aaaaataaa gaaaacaaa 
      ccggtggaa gtaaaaaaa gatgcgggc cccaaaaaa 
      gcgaaagtt caagataag gtgcaggag gccaagcct 
      aaggcgccc aaatcgctc gaaagtgca cttaatttg 
      attgacagt gatgcgttc aaaaacatc tttgagaaa 
      aacaagacg caatttccc gacgcccct atcgtacat 
      gattccgtt tttgcctca aaacccgat aactatccg 
      ttgaaaatt gtgccctct ggagttcta tctgtgatt 
      gagaaagtg gcccaagtt gtggataaa aacaacctt 
      ttggtgttt tacgacatt gccttaaca tcaatggcc 
      actgatatg agcaaaaat ttgccagcg ttaggttac 
      aagttgttt ctgcaacac atcgcctta aaggaacct 
      aaattaatt cgtgccaat ttggggaaa tattgtgtc 
      ttgcgaacg tcttatcag aaccgacca aacatcgga 
      ttatcaatt ttatgggct gtaggacag gctgggatt 
      agggatttt cagacggga ttagctatt tttcaggag 
      ttaatgtta ccgttaatt gaattgaaa aattattca 
      cgatttatc gtccagtat ttgattaag ttaatttcg 
      ggggctgac caagcgaca gttagtaaa gaacagttc 
      gtttcactt cttgatgtt gtgttttcg agcaagaaa 
      aactttccc agtgatctt aaacaagat ctcgtgaca 
      gttgtacca actttgaag tcgttccta gcgaccagt 
      aaagagcca acttgtgtc gacttttat ttgaagaaa 
      ataccaact gttggaaac gactcgttt aaagactgt 
      ttatgtgac gttatcgtt gatggtttt aatcgagat 
      cagagcagt ttggagagt tggggaaaa aattatacg 
      aaaaattgg caaactctt tcttgtaaa ataaataag 
      aaagtgttg gcgcataca cttaaccaa attcttctc 
      gaaagtaac gaattgagg actaggaaa aagaaagac 
      gaaggtttg attgaagcc attcgtgta tcagaggaa 
      ttattgggg aaaatggcc aataagaaa agctcccgt 
      tcgtggttc aaattcctc ttttacaca acgacttta 
      ttgctcgca atttacatt tatgctgac accaaacaa 
      gccggcagt tggcaaaaa tccaacaca aggaaattc 
      ctggtagag acaggtgtg tatgactac acgcataaa 
      gctgtgggt aaagtccaa gaaggttgg ctggtagtg 
      gacaacaaa atcaaggaa aattttcct acttatcgc 
      caagcggtt attgagttc tcggaacct tatattgag 
      ttttttaat agttttgga caaattctc tgcaatttg 
      ttcgccaat atcaaggag gctgtcatc gagaagtac 
      ccagtggtc gttaaatcg attgactca tatgctcct 
      ggcgttgtg gagcagtcc cagaacgca gtgagcaca 
      gcgtggtca agttctgta ttttatgtc aacagaagc 
      attgattat ttacgtacg gaagttttt gtcggccag 
      ttatctcct gaaaatatg caaagggtg gtgtatgag 
      gctttcaac accactcag accaaggcg actgaatat 
      taccactgg ttgtatgaa aaggtccaa aatacgatc 
      aaataa
    mRNA sequence
    • >TC009435 RA
      gtgcggtta tttcgtttt tgtagttga ttattttgt 
      gtaataatt gagtgaaag aaatgtcgg gtcaatggg 
      aagttgttg gcaaaaaaa aggaaaagg gagccaagg 
      atttaagtc aaaaagttg gcaaacaaa gtagaaaca 
      aaccggaaa atttgaatg taaaagttg aggatgttt 
      tggcgaaat cccaagtca aaaacctgt attccaacg 
      gaaaaaata aagaaaaca aaccggtgg aagtaaaaa 
      aagatgcgg gccccaaaa aagcgaaag ttcaagata 
      aggtgcagg aggccaagc ctaaggcgc ccaaatcgc 
      tcgaaagtg cacttaatt tgattgaca gtgatgcgt 
      tcaaaaaca tctttgaga aaaacaaga cgcaatttc 
      ccgacgccc ctatcgtac atgattccg tttttgcct 
      caaaacccg ataactatc cgttgaaaa ttgtgccct 
      ctggagttc tatctgtga ttgagaaag tggcccaag 
      ttgtggata aaaacaacc ttttggtgt tttacgaca 
      ttgccttaa catcaatgg ccactgata tgagcaaaa 
      atttgccag cgttaggtt acaagttgt ttctgcaac 
      acatcgcct taaaggaac ctaaattaa ttcgtgcca 
      atttgggga aatattgtg tcttgcgaa cgtcttatc 
      agaaccgac caaacatcg gattatcaa ttttatggg 
      ctgtaggac aggctggga ttagggatt ttcagacgg 
      gattagcta tttttcagg agttaatgt taccgttaa 
      ttgaattga aaaattatt cacgattta tcgtccagt 
      atttgatta agttaattt cgggggctg accaagcga 
      cagttagta aagaacagt tcgtttcac ttcttgatg 
      ttgtgtttt cgagcaaga aaaactttc ccagtgatc 
      ttaaacaag atctcgtga cagttgtac caactttga 
      agtcgttcc tagcgacca gtaaagagc caacttgtg 
      tcgactttt atttgaaga aaataccaa ctgttggaa 
      acgactcgt ttaaagact gtttatgtg acgttatcg 
      ttgatggtt ttaatcgag atcagagca gtttggaga 
      gttggggaa aaaattata gtaagaacg tcacctcaa 
      gtgccattc ttcttaaat atattgcga aaaattggc 
      aaactcttt cttgtaaaa taaataaga aagtgttgg 
      cgcatacac ttaaccaaa ttcttctcg aaagtaacg 
      aattgagga ctaggaaaa agaaagacg aaggtttga 
      ttgaagcca ttcgtgtat cagaggaat tattgggga 
      aaatggcca ataagaaaa gctcccgtt cgtggttca 
      aattcctct tttacacaa cgactttat tgctcgcaa 
      tttacattt atgctgaca ccaaacaag ccggcagtt 
      ggcaaaaat ccaacacaa ggaaattcc tggtagaga 
      caggtgtgt atgactaca cgcataaag ctgtgggta 
      aagtccaag aaggttggc tggtagtgg acaacaaaa 
      tcaaggaaa attttccta cttatcgcc aagcggtta 
      ttgagttct cggaacctt atattgagt tttttaata 
      gttttggac aaattctct gcaatttgt tcgccaata 
      tcaaggagg ctgtcatcg agaagtacc cagtggtcg 
      ttaaatcga ttgactcat atgctcctg gcgttgtgg 
      agcagtccc agaacgcag tgagcacag cgtggtcaa 
      gttctgtat tttatgtca acagaagca ttgattatt 
      tacgtacgg aagtttttg tcggccagt tatctcctg 
      aaaatatgc aaagggtgg tgtatgagg ctttcaaca 
      ccactcaga ccaaggcga ctgaatatt accactggt 
      tgtatgaaa aggtccaaa atacgatca aataagggc 
      tgtgagatg ggtgtctga ttgttacta ggttatggt 
      tagttgtat ttttttatt caattatac ttatttatt 
      ttacaaaac acattcctc ggagcattt attagagtg 
      atgtgttat tcgcatcag ggaagaata atttgagtg 
      tattttcgt aaatcagtt tagataaag tgtcagggt 
      tttcgtttt taacaacag caataacat caaggactt 
      gcctgaata tttagtt
    • >TC009435 RB
      gtgcggtta tttcgtttt tgtagttga ttattttgt 
      gtaataatt gagtgaaag aaatgtcgg gtcaatggg 
      aagttgttg gcaaaaaaa aggaaaagg gagccaagg 
      atttaagtc aaaaagttg gcaaacaaa gtagaaaca 
      aaccggaaa atttgaatg taaaagttg aggatgttt 
      tggcgaaat cccaagtca aaaacctgt attccaacg 
      gaaaaaata aagaaaaca aaccggtgg aagtaaaaa 
      aagatgcgg gccccaaaa aagcgaaag ttcaagata 
      aggtgcagg aggccaagc ctaaggcgc ccaaatcgc 
      tcgaaagtg cacttaatt tgattgaca gtgatgcgt 
      tcaaaaaca tctttgaga aaaacaaga cgcaatttc 
      ccgacgccc ctatcgtac atgattccg tttttgcct 
      caaaacccg ataactatc cgttgaaaa ttgtgccct 
      ctggagttc tatctgtga ttgagaaag tggcccaag 
      ttgtggata aaaacaacc ttttggtgt tttacgaca 
      ttgccttaa catcaatgg ccactgata tgagcaaaa 
      atttgccag cgttaggtt acaagttgt ttctgcaac 
      acatcgcct taaaggaac ctaaattaa ttcgtgcca 
      atttgggga aatattgtg tcttgcgaa cgtcttatc 
      agaaccgac caaacatcg gattatcaa ttttatggg 
      ctgtaggac aggctggga ttagggatt ttcagacgg 
      gattagcta tttttcagg agttaatgt taccgttaa 
      ttgaattga aaaattatt cacgattta tcgtccagt 
      atttgatta agttaattt cgggggctg accaagcga 
      cagttagta aagaacagt tcgtttcac ttcttgatg 
      ttgtgtttt cgagcaaga aaaactttc ccagtgatc 
      ttaaacaag atctcgtga cagttgtac caactttga 
      agtcgttcc tagcgacca gtaaagagc caacttgtg 
      tcgactttt atttgaaga aaataccaa ctgttggaa 
      acgactcgt ttaaagact gtttatgtg acgttatcg 
      ttgatggtt ttaatcgag atcagagca gtttggaga 
      gttggggaa aaaattata cgaaaaatt ggcaaactc 
      tttcttgta aaataaata agaaagtgt tggcgcata 
      cacttaacc aaattcttc tcgaaagta acgaattga 
      ggactagga aaaagaaag acgaaggtt tgattgaag 
      ccattcgtg tatcagagg aattattgg ggaaaatgg 
      ccaataaga aaagctccc gttcgtggt tcaaattcc 
      tcttttaca caacgactt tattgctcg caatttaca 
      tttatgctg acaccaaac aagccggca gttggcaaa 
      aatccaaca caaggaaat tcctggtag agacaggtg 
      tgtatgact acacgcata aagctgtgg gtaaagtcc 
      aagaaggtt ggctggtag tggacaaca aaatcaagg 
      aaaattttc ctacttatc gccaagcgg ttattgagt 
      tctcggaac cttatattg agtttttta atagttttg 
      gacaaattc tctgcaatt tgttcgcca atatcaagg 
      aggctgtca tcgagaagt acccagtgg tcgttaaat 
      cgattgact catatgctc ctggcgttg tggagcagt 
      cccagaacg cagtgagca cagcgtggt caagttctg 
      tattttatg tcaacagaa gcattgatt atttacgta 
      cggaagttt ttgtcggcc agttatctc ctgaaaata 
      tgcaaaggg tggtgtatg aggctttca acaccactc 
      agaccaagg cgactgaat attaccact ggttgtatg 
      aaaaggtcc aaaatacga tcaaataag ggctgtgag 
      atgggtgtc tgattgtta ctaggttat ggttagttg 
      tattttttt attcaatta tacttattt attttacaa 
      aacacattc ctcggagca tttattaga gtgatgtgt 
      tattcgcat cagggaaga ataatttga gtgtatttt 
      cgtaaatca gtttagata aagtgtcag ggttttcgt 
      ttttaacaa cagcaataa catcaagga cttgcctga 
      atatttagt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY