General information | |||
TC009425 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1398749..1405987 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009425 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04631 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
ovary without detectable defects - Number of animals showing the phenotype: 1
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
larva appendages partially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
maxillary palp shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
labium shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs coxa size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs trochanter size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs femur size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
- >TC009425 RA
MGDYQVTGV QQDAMGAVQ LELGGNPNA LALRRPFDP LAHDLEASF RLTRFADLK GRGCKVPQE VLNKLVEGL QQDYNTAAE HEHAHFMHM AIPRIGIGL DCSVTPVRH GGLCLVQTT DFFYPLVDD PYMMGKIAC ANVLSDLYA MGVTECDNM LMLLGVSTK MTEKERDVV IPLIMRGFK DSALEAGTT VTGGQTVVN PWCTIGGIA TTVCQPNEY IVPDNAVVG DVLVLTKPL GTQVAVNAH QWLDQPERW NRIKLVVSE EDVRKAYHR AMDSMARLN RIASRLMHK YNAHGATDV TGFGLLGHA QTLASHQKN EVSFVIHNL PVIAKMAAV AKACGNMFQ LLQGHSAET SGGLLICLP REQAAAYCK DIEKQEGYQ AWIIGIVEK GNRTARIID KPRVIEVPA KE
- >TC009425 RA
atgggtgat taccaagtg actggggtc cagcaggat gcgatgggg gctgtccag ctggagctg ggtggaaat ccgaatgct cttgctttg cggcggcct tttgaccct ttggcgcac gacttggag gccagtttc cggctaacg cgctttgcc gacctcaag ggacgtggc tgcaaggtg ccccaggag gtgctcaac aagctggtg gaggggctc cagcaggac tacaacacg gccgccgaa cacgaacat gcccacttt atgcatatg gccataccc cgcatcggc atcggactc gattgctcg gtgacgccg gtgcgccat gggggcctg tgcttggtc cagacgaca gacttcttc tacccactg gtggacgac ccgtacatg atggggaag atcgcttgt gccaatgtc ctgagtgac ttgtatgcc atgggggtg acagagtgc gacaatatg ctgatgttg ttgggggtc agtacgaag atgactgaa aaggagcga gatgttgtg attccccta attatgaga ggtttcaaa gattctgcc ctggaagct ggaacaact gtaacaggt ggacaaacc gttgtgaat ccctggtgt acgattggt ggtattgcc acaactgtc tgtcaaccg aatgaatat atcgtgccg gataatgcc gttgttgga gatgtgctc gtactcacc aagccactt ggaacgcaa gtggccgtc aacgcacac cagtggttg gaccagccc gagcgctgg aaccgaata aagttagtt gtaagtgaa gaggacgtg cgtaaagcg tatcatcgt gcaatggac tcgatggcg cggttgaat cgcatcgct agcagattg atgcataaa tataatgcc catggagct acagatgtg acaggcttt ggtctgtta ggtcacgca caaacgtta gcttcgcat cagaagaac gaggtttcg ttcgttatt cataacttg cccgtaatt gcgaaaatg gcagccgta gccaaggct tgcggtaac atgttccaa cttttgcaa ggacattcg gctgaaacg tcaggagga cttttgatc tgtttgccg cgagaacaa gcggccgct tattgtaaa gatatagaa aaacaagaa ggttatcaa gcttggatc attgggatt gttgaaaag gggaaccga actgctagg attattgat aagcctcgg gttattgaa gttccggct aaagaataa
- >TC009425 RA
gttctgtga aacatttaa aatttattc aatgtgatg atgatgcgt aaattaaca aagttattt acgaaaata ttttacctt aggcacctg aaaaatcga taaatcatt tgttaagag ctttatctg accacacag tcgacattt cttcatttt tacaacgaa tttacaaaa aaatttgtc aatttgata actccagtg acagtccta tgacagtcc cagtctacc gtgctgcca tctatctac ttgctccaa cgttagatt caaataaaa tggcgactc agtttacct gtctgtcta atatcgtgg ttgaccgtt gacagttga cagttaaca ccgcagcag caactaaca attttcagt atttcaatt ttcagtgca ttacacgtt attctacgg tgcatcata ggtgtttaa tcgttgtta ataggtcac agtgtggca cttacaccc cgggggctc ttttttgcg tggcttgtt tgggttttg tttgtttga aatcgaacc gttgttgaa aattttgac aacctcgtg aatagttgc gcatcgcga acggaccaa tgggatggt tgctcggtt ctggtcatg cgcagaacc ggtgggctg tggaggaag aggtagcca ttttgtgag cgtcggttg attgtttga gaaacgctc tgttgacga gctagtgtt tttaacacg ctagtaccc agcataaag tttcaaagc cggtatggg tgattacca agtgactgg ggtccagca ggatgcgat gggggctgt ccagctgga gctgggtgg aaatccgaa tgctcttgc tttgcggcg gccttttga ccctttggc gcacgactt ggaggccag tttccggct aacgcgctt tgccgacct caagggacg tggctgcaa ggtgcccca ggaggtgct caacaagct ggtggaggg gctccagca ggactacaa cacggccgc cgaacacga acatgccca ctttatgca tatggccat accccgcat cggcatcgg actcgattg ctcggtgac gccggtgcg ccatggggg cctgtgctt ggtccagac gacagactt cttctaccc actggtgga cgacccgta catgatggg gaagatcgc ttgtgccaa tgtcctgag tgacttgta tgccatggg ggtgacaga gtgcgacaa tatgctgat gttgttggg ggtcagtac gaagatgac tgaaaagga gcgagatgt tgtgattcc cctaattat gagaggttt caaagattc tgccctgga agctggaac aactgtaac aggtggaca aaccgttgt gaatccctg gtgtacgat tggtggtat tgccacaac tgtctgtca accgaatga atatatcgt gccggataa tgccgttgt tggagatgt gctcgtact caccaagcc acttggaac gcaagtggc cgtcaacgc acaccagtg gttggacca gcccgagcg ctggaaccg aataaagtt agttgtaag tgaagagga cgtgcgtaa agcgtatca tcgtgcaat ggactcgat ggcgcggtt gaatcgcat cgctagcag attgatgca taaatataa tgcccatgg agctacaga tgtgacagg ctttggtct gttaggtca cgcacaaac gttagcttc gcatcagaa gaacgaggt ttcgttcgt tattcataa cttgcccgt aattgcgaa aatggcagc cgtagccaa ggcttgcgg taacatgtt ccaactttt gcaaggaca ttcggctga aacgtcagg aggactttt gatctgttt gccgcgaga acaagcggc cgcttattg taaagatat agaaaaaca agaaggtta tcaagcttg gatcattgg gattgttga aaaggggaa ccgaactgc taggattat tgataagcc tcgggttat tgaagttcc ggctaaaga ataagtaca tttcaatat tccaagtat tgatgttta tgactggcc gttaatttt ctagctgcc taggtcata agcgctatg aataacagt ttcaaccta aagtcgttt attttcaaa tatttttcc aagatgttc aaagaagtg catgtttat gaatactat ttttgttgt tgaatactg ttaccattc gcttcttgt agtttcaat attttccct taaagcagt ttataaaga tttatttag cttttgtta tcaaattta tttagtgat gcattttcc actaaaact aatctcaga ttctcgtta gtagactat gtatcttgt acaaaattt cgtttcgta tttatgttt cgtcataga gttttagaa ataggtaaa agtcgtatt ggtattttg tataataat ttttcaaat aattaagta ttctactga cactgtgca gtttttctt ccagttccc ccacacaaa acaaaag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
