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Gene information
General information
TC009425 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1398749..1405987
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009425
    iBeetle screen iB_04631

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    larva appendages partially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    maxillary palp shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labium shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs coxa size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs trochanter size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs femur size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009425 RA
      MGDYQVTGV QQDAMGAVQ LELGGNPNA LALRRPFDP 
      LAHDLEASF RLTRFADLK GRGCKVPQE VLNKLVEGL 
      QQDYNTAAE HEHAHFMHM AIPRIGIGL DCSVTPVRH 
      GGLCLVQTT DFFYPLVDD PYMMGKIAC ANVLSDLYA 
      MGVTECDNM LMLLGVSTK MTEKERDVV IPLIMRGFK 
      DSALEAGTT VTGGQTVVN PWCTIGGIA TTVCQPNEY 
      IVPDNAVVG DVLVLTKPL GTQVAVNAH QWLDQPERW 
      NRIKLVVSE EDVRKAYHR AMDSMARLN RIASRLMHK 
      YNAHGATDV TGFGLLGHA QTLASHQKN EVSFVIHNL 
      PVIAKMAAV AKACGNMFQ LLQGHSAET SGGLLICLP 
      REQAAAYCK DIEKQEGYQ AWIIGIVEK GNRTARIID 
      KPRVIEVPA KE
    CDS sequence
    • >TC009425 RA
      atgggtgat taccaagtg actggggtc cagcaggat 
      gcgatgggg gctgtccag ctggagctg ggtggaaat 
      ccgaatgct cttgctttg cggcggcct tttgaccct 
      ttggcgcac gacttggag gccagtttc cggctaacg 
      cgctttgcc gacctcaag ggacgtggc tgcaaggtg 
      ccccaggag gtgctcaac aagctggtg gaggggctc 
      cagcaggac tacaacacg gccgccgaa cacgaacat 
      gcccacttt atgcatatg gccataccc cgcatcggc 
      atcggactc gattgctcg gtgacgccg gtgcgccat 
      gggggcctg tgcttggtc cagacgaca gacttcttc 
      tacccactg gtggacgac ccgtacatg atggggaag 
      atcgcttgt gccaatgtc ctgagtgac ttgtatgcc 
      atgggggtg acagagtgc gacaatatg ctgatgttg 
      ttgggggtc agtacgaag atgactgaa aaggagcga 
      gatgttgtg attccccta attatgaga ggtttcaaa 
      gattctgcc ctggaagct ggaacaact gtaacaggt 
      ggacaaacc gttgtgaat ccctggtgt acgattggt 
      ggtattgcc acaactgtc tgtcaaccg aatgaatat 
      atcgtgccg gataatgcc gttgttgga gatgtgctc 
      gtactcacc aagccactt ggaacgcaa gtggccgtc 
      aacgcacac cagtggttg gaccagccc gagcgctgg 
      aaccgaata aagttagtt gtaagtgaa gaggacgtg 
      cgtaaagcg tatcatcgt gcaatggac tcgatggcg 
      cggttgaat cgcatcgct agcagattg atgcataaa 
      tataatgcc catggagct acagatgtg acaggcttt 
      ggtctgtta ggtcacgca caaacgtta gcttcgcat 
      cagaagaac gaggtttcg ttcgttatt cataacttg 
      cccgtaatt gcgaaaatg gcagccgta gccaaggct 
      tgcggtaac atgttccaa cttttgcaa ggacattcg 
      gctgaaacg tcaggagga cttttgatc tgtttgccg 
      cgagaacaa gcggccgct tattgtaaa gatatagaa 
      aaacaagaa ggttatcaa gcttggatc attgggatt 
      gttgaaaag gggaaccga actgctagg attattgat 
      aagcctcgg gttattgaa gttccggct aaagaataa
    mRNA sequence
    • >TC009425 RA
      gttctgtga aacatttaa aatttattc aatgtgatg 
      atgatgcgt aaattaaca aagttattt acgaaaata 
      ttttacctt aggcacctg aaaaatcga taaatcatt 
      tgttaagag ctttatctg accacacag tcgacattt 
      cttcatttt tacaacgaa tttacaaaa aaatttgtc 
      aatttgata actccagtg acagtccta tgacagtcc 
      cagtctacc gtgctgcca tctatctac ttgctccaa 
      cgttagatt caaataaaa tggcgactc agtttacct 
      gtctgtcta atatcgtgg ttgaccgtt gacagttga 
      cagttaaca ccgcagcag caactaaca attttcagt 
      atttcaatt ttcagtgca ttacacgtt attctacgg 
      tgcatcata ggtgtttaa tcgttgtta ataggtcac 
      agtgtggca cttacaccc cgggggctc ttttttgcg 
      tggcttgtt tgggttttg tttgtttga aatcgaacc 
      gttgttgaa aattttgac aacctcgtg aatagttgc 
      gcatcgcga acggaccaa tgggatggt tgctcggtt 
      ctggtcatg cgcagaacc ggtgggctg tggaggaag 
      aggtagcca ttttgtgag cgtcggttg attgtttga 
      gaaacgctc tgttgacga gctagtgtt tttaacacg 
      ctagtaccc agcataaag tttcaaagc cggtatggg 
      tgattacca agtgactgg ggtccagca ggatgcgat 
      gggggctgt ccagctgga gctgggtgg aaatccgaa 
      tgctcttgc tttgcggcg gccttttga ccctttggc 
      gcacgactt ggaggccag tttccggct aacgcgctt 
      tgccgacct caagggacg tggctgcaa ggtgcccca 
      ggaggtgct caacaagct ggtggaggg gctccagca 
      ggactacaa cacggccgc cgaacacga acatgccca 
      ctttatgca tatggccat accccgcat cggcatcgg 
      actcgattg ctcggtgac gccggtgcg ccatggggg 
      cctgtgctt ggtccagac gacagactt cttctaccc 
      actggtgga cgacccgta catgatggg gaagatcgc 
      ttgtgccaa tgtcctgag tgacttgta tgccatggg 
      ggtgacaga gtgcgacaa tatgctgat gttgttggg 
      ggtcagtac gaagatgac tgaaaagga gcgagatgt 
      tgtgattcc cctaattat gagaggttt caaagattc 
      tgccctgga agctggaac aactgtaac aggtggaca 
      aaccgttgt gaatccctg gtgtacgat tggtggtat 
      tgccacaac tgtctgtca accgaatga atatatcgt 
      gccggataa tgccgttgt tggagatgt gctcgtact 
      caccaagcc acttggaac gcaagtggc cgtcaacgc 
      acaccagtg gttggacca gcccgagcg ctggaaccg 
      aataaagtt agttgtaag tgaagagga cgtgcgtaa 
      agcgtatca tcgtgcaat ggactcgat ggcgcggtt 
      gaatcgcat cgctagcag attgatgca taaatataa 
      tgcccatgg agctacaga tgtgacagg ctttggtct 
      gttaggtca cgcacaaac gttagcttc gcatcagaa 
      gaacgaggt ttcgttcgt tattcataa cttgcccgt 
      aattgcgaa aatggcagc cgtagccaa ggcttgcgg 
      taacatgtt ccaactttt gcaaggaca ttcggctga 
      aacgtcagg aggactttt gatctgttt gccgcgaga 
      acaagcggc cgcttattg taaagatat agaaaaaca 
      agaaggtta tcaagcttg gatcattgg gattgttga 
      aaaggggaa ccgaactgc taggattat tgataagcc 
      tcgggttat tgaagttcc ggctaaaga ataagtaca 
      tttcaatat tccaagtat tgatgttta tgactggcc 
      gttaatttt ctagctgcc taggtcata agcgctatg 
      aataacagt ttcaaccta aagtcgttt attttcaaa 
      tatttttcc aagatgttc aaagaagtg catgtttat 
      gaatactat ttttgttgt tgaatactg ttaccattc 
      gcttcttgt agtttcaat attttccct taaagcagt 
      ttataaaga tttatttag cttttgtta tcaaattta 
      tttagtgat gcattttcc actaaaact aatctcaga 
      ttctcgtta gtagactat gtatcttgt acaaaattt 
      cgtttcgta tttatgttt cgtcataga gttttagaa 
      ataggtaaa agtcgtatt ggtattttg tataataat 
      ttttcaaat aattaagta ttctactga cactgtgca 
      gtttttctt ccagttccc ccacacaaa acaaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY