Status
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Gene information
General information
TC009391 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1462758..1466178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009391
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04627

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence -

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009391 RA
      MAEEIKHLP MVVPLPLPE AQLVEIVEK SKDWALMHG 
      AAMRSKTNF SEDALQFAP FVLIPSAFP RKEFAKALE 
      IQLILNELM HKVAHDRRF LTESLKETI KVDEFTGNL 
      FRIYETVHS EGLTQFGYG SVRFDVGGI VQKAHADLG 
      ALLLLEAGG VQHDRVGFR LAGAVFRGH PERASLGLL 
      RSDYMVNSA STDKIKQVE INTIASSFA GIGTNITVF 
      HRYILQELG HSEMLKNLP ENNALEGLC SGMIEAWKI 
      YGNPKAVIM FLIEDITYN ICDQRFHEF KIREMCPQV 
      RVIRRTLTD MADRASLGP KKQLLIDGC EVAVIYFRA 
      GYEPGHYHS QKEWDARLL MERSLAIKS PSIHYHLAG 
      TKKVQQTLA KPGALEQFL SDPVKVEKV KQIFAGLYS 
      LDFDELGEQ AIQMAIDDP ERFVLKPQR EGGGNNVYG 
      LEVRDAVKK MKDSEERTA WILMERIHP PLSTGYMVR 
      PGGSKVPQL VDMVSELGI FGVVIGDAE KITYNKQVG 
      HMLRTKVAT ANEGGVAAG LGALDSPYL ID
    CDS sequence
    • >TC009391 RA
      atggccgaa gagatcaag catttgccc atggtggtg 
      cccttgccc ttgccggag gcccagctc gtcgaaatc 
      gtggagaag tccaaagac tgggccctc atgcatggg 
      gctgccatg cgctccaag accaacttc tcggaggac 
      gccctccag ttcgccccc tttgtgctg atcccgtcg 
      gcgttcccg cgcaaggag ttcgccaag gcgctcgaa 
      atccagctg attttgaac gagctgatg cacaaggtg 
      gcccacgac cggcgcttc ctcaccgag agcctcaaa 
      gagacgatc aaagtggac gagttcacg gggaatctg 
      ttccgcatt tacgagacg gtgcactcc gaggggctc 
      acacagttt gggtatggt tcggtcaga tttgatgtt 
      ggagggatc gtgcagaag gcacacgcc gacctcggc 
      gcccttctg ctgctggaa gcaggtgga gttcaacac 
      gatcgcgtc gggtttcgg ctggctggg gccgtcttc 
      aggggccat ccagagcgc gcctctttg ggactgctg 
      cggtctgat tacatggtg aattcggcg tcgactgac 
      aaaattaaa caagtcgaa ataaatacg attgcctcg 
      agcttcgca ggcatcggc acaaacatt acggtattc 
      cataggtac attttacaa gaattgggt cacagcgaa 
      atgctcaaa aacttgccg gaaaataat gcactcgaa 
      ggcctttgt agtggcatg attgaagca tggaaaatt 
      tacggtaac cccaaagcc gtcatcatg ttcctaatc 
      gaagacatt acttacaat atttgcgat caaagattt 
      cacgaattt aaaatcagg gaaatgtgt cctcaggtt 
      cgtgtcatt cggcgcact ttgaccgat atggcagat 
      agggcgagt ttagggccg aaaaaacaa ctgttgata 
      gatgggtgc gaggttgcg gttatttat tttagagct 
      gggtacgaa ccggggcat tatcacagt cagaaggaa 
      tgggacgcc aggctttta atggaaagg tcgctggct 
      ataaagagt ccctcgatt cactatcac ttagctggg 
      acgaaaaaa gtccaacaa accctagct aagccaggg 
      gctttggaa caatttttg agcgaccct gtgaaggtt 
      gaaaaagtc aagcagatt tttgcgggt ctctacagt 
      cttgatttt gacgaactg ggggaacaa gcgattcaa 
      atggccatt gatgacccg gaaagattc gttttaaag 
      ccccagagg gaaggcggt ggtaataat gtgtacggg 
      ttggaggtg cgagacgct gttaaaaaa atgaaagat 
      agtgaagaa cgcacggct tggattttg atggaacgg 
      attcatccg ccgttatct acggggtat atggtgcga 
      cctgggggt agtaaggtt ccacagttg gtggacatg 
      gtttcggag ttggggatt tttggggtc gtgattggg 
      gatgcagaa aaaatcacg tataataaa caagtgggg 
      catatgctg cggactaaa gtggcaact gcgaacgaa 
      ggtggggtg gctgcgggt ttgggtgcc ttagatagc 
      ccatatttg atagactag
    mRNA sequence
    • >TC009391 RA
      taacttcac gactgttta atttgattg tcaaattca 
      atgtcagcg attatcgca agacaataa catgttaag 
      taagaaatc agaaaaaca tagtggaga acacttgat 
      aagataacg cagatttgt ccgaggacc cctccaact 
      gtgatttca ttgcatgtc agtgcctca gttcccagc 
      aaaaagccg aagcaagta cccttatca gaatggccg 
      aagagatca agcatttgc ccatggtgg tgcccttgc 
      ccttgccgg aggcccagc tcgtcgaaa tcgtggaga 
      agtccaaag actgggccc tcatgcatg gggctgcca 
      tgcgctcca agaccaact tctcggagg acgccctcc 
      agttcgccc cctttgtgc tgatcccgt cggcgttcc 
      cgcgcaagg agttcgcca aggcgctcg aaatccagc 
      tgattttga acgagctga tgcacaagg tggcccacg 
      accggcgct tcctcaccg agagcctca aagagacga 
      tcaaagtgg acgagttca cggggaatc tgttccgca 
      tttacgaga cggtgcact ccgaggggc tcacacagt 
      ttgggtatg gttcggtca gatttgatg ttggaggga 
      tcgtgcaga aggcacacg ccgacctcg gcgcccttc 
      tgctgctgg aagcaggtg gagttcaac acgatcgcg 
      tcgggtttc ggctggctg gggccgtct tcaggggcc 
      atccagagc gcgcctctt tgggactgc tgcggtctg 
      attacatgg tgaattcgg cgtcgactg acaaaatta 
      aacaagtcg aaataaata cgattgcct cgagcttcg 
      caggcatcg gcacaaaca ttacggtat tccataggt 
      acattttac aagaattgg gtcacagcg aaatgctca 
      aaaacttgc cggaaaata atgcactcg aaggccttt 
      gtagtggca tgattgaag catggaaaa tttacggta 
      accccaaag ccgtcatca tgttcctaa tcgaagaca 
      ttacttaca atatttgcg atcaaagat ttcacgaat 
      ttaaaatca gggaaatgt gtcctcagg ttcgtgtca 
      ttcggcgca ctttgaccg atatggcag atagggcga 
      gtttagggc cgaaaaaac aactgttga tagatgggt 
      gcgaggttg cggttattt attttagag ctgggtacg 
      aaccggggc attatcaca gtcagaagg aatgggacg 
      ccaggcttt taatggaaa ggtcgctgg ctataaaga 
      gtccctcga ttcactatc acttagctg ggacgaaaa 
      aagtccaac aaaccctag ctaagccag gggctttgg 
      aacaatttt tgagcgacc ctgtgaagg ttgaaaaag 
      tcaagcaga tttttgcgg gtctctaca gtcttgatt 
      ttgacgaac tgggggaac aagcgattc aaatggcca 
      ttgatgacc cggaaagat tcgttttaa agccccaga 
      gggaaggcg gtggtaata atgtgtacg ggttggagg 
      tgcgagacg ctgttaaaa aaatgaaag atagtgaag 
      aacgcacgg cttggattt tgatggaac ggattcatc 
      cgccgttat ctacggggt atatggtgc gacctgggg 
      gtagtaagg ttccacagt tggtggaca tggtttcgg 
      agttgggga tttttgggg tcgtgattg gggatgcag 
      aaaaaatca cgtataata aacaagtgg ggcatatgc 
      tgcggacta aagtggcaa ctgcgaacg aaggtgggg 
      tggctgcgg gtttgggtg ccttagata gcccatatt 
      tgatagact agcatttgt acgcaagtt gataataaa 
      actatcaca cagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY