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Gene information
General information
TC009387 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1396925..1398745
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009387
    iBeetle screen iB_04625

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009387 RA
      MVKTLDKQK LIIKDALTN VDGKPNKKI IFRENLDDS 
      SQLNGASSH HEPQKSLFE SDDEAEDDL RFELKPQFS 
      GKKGHQLFE LQTKYKGDK RFALDERFA DANDEEENV 
      VNGECSLED EKKHELNIL EQVLGTKIK TKDVKVQED 
      GSKKKRMVR YDPSQSDHV KFHVSVTQD ESAPKTKKK 
      KKLKDAQKE DSNIQEQEQ PVVSKEIFY KVEDGLKST 
      LEEKQEFSL LKLFGQEEK DELNNSIEN YETQTNNKI 
      RNNAFLNDN PFHYDSSDD EPEEPKNAE TTKKPEEFE 
      TKAQTNTVR TWFEPFFLK EDDYRLQEG SDFIKRLAS 
      ENKSEFSQQ RRDIKLIVR AKVRNNLRK NNQFKKKLG 
      GNKRKKMIR IKKAMKK
    CDS sequence
    • >TC009387 RA
      atggttaaa acccttgat aagcaaaag ttgattata 
      aaagatgct ctcaccaac gtcgatgga aagcccaac 
      aaaaaaatt atttttcgc gaaaatctg gacgactcc 
      tctcagctt aacggtgcg agttcgcac catgagcct 
      caaaagtcg cttttcgaa agtgacgac gaagctgaa 
      gacgatttg aggtttgaa ttaaagcct cagtttagt 
      ggcaagaaa gggcaccaa ctttttgag ctacagacg 
      aaatacaag ggtgataaa aggtttgcg ttggacgaa 
      cggtttgct gatgctaat gacgaagag gaaaatgtc 
      gtaaatggc gagtgctcg cttgaagac gagaagaaa 
      cacgagttg aatatttta gagcaagtt ttgggaacc 
      aaaataaag acgaaagat gttaaggtg caagaggat 
      gggtcaaag aaaaagcga atggtgcgg tatgatccc 
      tcacagtca gatcatgtg aaattccat gtgagtgta 
      actcaagat gagtctgct ccaaaaacc aagaaaaag 
      aaaaaactg aaagatgca cagaaagaa gattccaat 
      attcaagag caagagcag ccagttgtt tcaaaagag 
      attttttat aaagtagaa gatggttta aaaagtaca 
      cttgaagag aaacaagaa tttagccta ttaaaattg 
      ttcggacag gaagaaaaa gacgaacta aataactcg 
      atcgaaaat tatgaaaca caaaccaac aataaaata 
      cggaataac gctttcctg aatgacaat cccttccat 
      tacgattcg tcagacgat gaaccggaa gaacccaaa 
      aatgcggaa actacgaaa aaacctgaa gaatttgaa 
      acaaaggct cagacaaac actgtaaga acgtggttt 
      gaaccattc ttcttgaag gaagatgat tatcgcttg 
      caagagggg tccgacttt atcaaaaga ttagcttca 
      gaaaacaaa tccgagttt agccagcag agaagagat 
      ataaagctt attgttagg gcaaaagtg cgaaacaat 
      ctaagaaaa aataaccag ttcaagaaa aaacttgga 
      ggaaataaa cggaaaaag atgattaga ataaagaaa 
      gcaatgaag aagtaa
    mRNA sequence
    • >TC009387 RA
      aaaataacc caacacctg atttttgcc ataatagtt 
      aaaaaaaaa agaaaaact accctctga tggtatttc 
      aaccatttg gataatggt tccttccaa cttggggcg 
      caaattttc cgaaatcac aagaaacca ccgtcatgg 
      agataaatg taccataaa agcggcatc catcacagt 
      aacgtcata ccatcatgg tttcatctc ttccataac 
      agttgtttg tgcaattac tgcaatttc cccgcattt 
      tctcgtctt tagtccaaa accatccat ttactcgtg 
      caattctaa tgcaattga attgcaatt tttgaaaat 
      tttttggaa aaaaccttt tcgaaagaa aaaatttaa 
      atcaagtta gggaaatta gttttgtaa ccgacttga 
      cataacctg gttttaaca taaccttac cgttataac 
      ctcgttatt ttataattt tgacgaaat ggttaaaac 
      ccttgataa gcaaaagtt gattataaa agatgctct 
      caccaacgt cgatggaaa gcccaacaa aaaaattat 
      ttttcgcga aaatctgga cgactcctc tcagcttaa 
      cggtgcgag ttcgcacca tgagcctca aaagtcgct 
      tttcgaaag tgacgacga agctgaaga cgatttgag 
      gtttgaatt aaagcctca gtttagtgg caagaaagg 
      gcaccaact ttttgagct acagacgaa atacaaggg 
      tgataaaag gtttgcgtt ggacgaacg gtttgctga 
      tgctaatga cgaagagga aaatgtcgt aaatggcga 
      gtgctcgct tgaagacga gaagaaaca cgagttgaa 
      tattttaga gcaagtttt gggaaccaa aataaagac 
      gaaagatgt taaggtgca agaggatgg gtcaaagaa 
      aaagcgaat ggtgcggta tgatccctc acagtcaga 
      tcatgtgaa attccatgt gagtgtaac tcaagatga 
      gtctgctcc aaaaaccaa gaaaaagaa aaaactgaa 
      agatgcaca gaaagaaga ttccaatat tcaagagca 
      agagcagcc agttgtttc aaaagagat tttttataa 
      agtagaaga tggtttaaa aagtacact tgaagagaa 
      acaagaatt tagcctatt aaaattgtt cggacagga 
      agaaaaaga cgaactaaa taactcgat cgaaaatta 
      tgaaacaca aaccaacaa taaaatacg gaataacgc 
      tttcctgaa tgacaatcc cttccatta cgattcgtc 
      agacgatga accggaaga acccaaaaa tgcggaaac 
      tacgaaaaa acctgaaga atttgaaac aaaggctca 
      gacaaacac tgtaagaac gtggtttga accattctt 
      cttgaagga agatgatta tcgcttgca agaggggtc 
      cgactttat caaaagatt agcttcaga aaacaaatc 
      cgagtttag ccagcagag aagagatat aaagcttat 
      tgttagggc aaaagtgcg aaacaatct aagaaaaaa 
      taaccagtt caagaaaaa acttggagg aaataaacg 
      gaaaaagat gattagaat aaagaaagc aatgaagaa 
      gtaacattt ataaactta attttttaa taaaatttt 
      tactcgtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY