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Gene information
General information
TC009376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1172776..1181865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009376
    iBeetle screen iB_04623

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    Pupal morphology
    labrum shortened - Number of animals showing the phenotype: 4

    elytra not closed - Number of animals showing the phenotype: 4

    Adult morphology
    prosternal process broader - Number of animals showing the phenotype: 2


    wings not present - Number of animals showing the phenotype: 1


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009376 RA
      MYFPIGWPK VIKIPDLGH ASVRQITCN RDRILFAIL 
TDDSIAIWF CKPCVPIVF YRRTPQSLE KFGTNILTE 
WKPDSSMVA VATSEGHLL LFKLGVIAD NKGLYVQTD 
SPHANLRRD SAELFIKEI IPPLHLTLH QEIMVWDGK 
ITGIVCITM SEFMISTSE GHVLRYWWD GQQHRDYNL 
DLRRIPFCI NQQVSKAIP IVEENTFIV DIEYSPLVG 
GFSIVLNDG RAAFLTASS LKFDPNQVQ GIWAQNIED 
ATCTVMNHK YRLITFGRA NSECIVYYV DESTGGLEV 
SHNCVLSSK DYPGDPGAV AQVLWTPDG CALVAAWEK 
GGIAMWSTF GSLLMCSLG WDYGLNIDL QTNNPLQIK 
SMQFATEGY QLWMVHKEA KDGEVNSNG TCTNLLQLD 
FMKSALTIN PCMSHQSHL YLQGEDKLY VNSADTLIK 
MFSERSTKD EGVFNESLS MPSTLAEGR QWLVIPVPS 
TYSATNWPI RYSAIDSDG QNMAIAGRT GLAHYSMQT 
RRWKLFGNE TQEKDFIVV GGLLWWRDY LVMGCYSIL 
ENSDELRFY PRDAKLDNK FAKIVTVLS PILLMNILQ 
DQLITFGSD AQVTIWALK HNPTVGNVE VFKVQVVDI 
SALAVHPAC IVSVTLSSL RTETGRGQP NNSESIVLN 
VSGRLLMVQ REVRNGERY TCSMPTVLA SCVENVWVP 
SRRKAEKAH LTEALWLFC GAHGMRVWL PLYPKDGDK 
THTFMSKRI MLPFHLKIY PLAILFEDA IILGAENDT 
VLYTSDSNS PFSLPFSVL QRTSQVYLH QILRQLIRR 
NLGYHAWEI ARSCMSLPY FPHSLELLL HEVLEEEAT 
SKEPIPDAQ LPSVIEFIM EFPVYLQTV VQCARKTEI 
ALWPYLFSA AGKPKDLFQ ECMAKRQLD TAASYLIIL 
QNLETSSVS RQYATLLLN TALDQSKWE LAKDLVRFL 
RAIDPNDVE SPRTSFILP PKLGISQQT PPVSPNAED 
LSLILGNVQ GPRVRSYST TISPKLVEN RPNNPTNHP 
IESPQTARK KSVPNTVGR NSPNNNTAE EFFIDVILQ 
KHARRLLST RRLTDLGYF AAHLDFHLV AWLGKERDR 
AARIDDFVQ ALKHLHEEF QWPYPTPSA FSPPNTGCD 
LEDRLKLLK IDVTHPASR VGDSGYMSF QGVPCTGLI 
TPINTLDSE TELDDLSQS ETNKIDDKG DLATSSTFY 
DKRIDLAQV GGRVDDAAM SSVSSVWGE DRIESSNDN 
WEVPSTQIL EQLSNTDNK GSQRSEVQL RYLLQLFME 
ASCLEWSLI ISVLLRDAM AVLRTVNAA KSPDQSIEA 
VSRLRQGLL LLQYWTNTE CLGYKAFML AIQNQISVL 
SKILETKIQ HQKIVEYQQ AVSASLNKL STPIPARSR 
KTSSNHDNS SQSGSVKEK KNINITTSH STTDKLNEM 
NPINSSSCA KTINQENNN QVEDAIIPE STGCVIS
    CDS sequence
    • >TC009376 RA
      atgtacttc ccgatcggt tggccaaaa gtcatcaaa 
ataccagat ttaggacat gcaagtgta aggcaaata 
acttgcaac agggacagg atactgttt gctatacta 
acggacgat tcgatcgcg atttggttt tgtaaacca 
tgcgttcca attgtcttc tatcgaaga accccacag 
tccttggaa aaattcgga acgaacatt ttaaccgaa 
tggaagccg gactccagc atggtggca gtcgcgacg 
tcagaaggc cacttgtta ttgtttaaa ctaggagtg 
atagctgat aataaaggt ttatacgtc cagactgat 
agcccccat gcgaattta cgtagggac agcgccgaa 
ttgttcatt aaagaaata attccgcct ctgcatctc 
actctgcac caagaaatt atggtgtgg gacggtaaa 
ataacagga atcgtctgt ataacaatg tcagagttt 
atgatctca acgagtgaa ggacatgtc ttgaggtat 
tggtgggat ggacaacaa catagagac tataatttg 
gatttgagg agaatacct ttctgtatc aatcaacaa 
gtgtccaaa gcaatccca attgttgaa gaaaatacg 
tttatagtt gatatagaa tattcgcca ctagttggc 
ggtttttca attgtttta aatgacgga agagcggcg 
tttttaaca gcctcctct ctcaaattt gatcccaat 
caagtccaa ggaatatgg gcccagaac atcgaagac 
gcaacttgc accgttatg aaccacaaa taccgatta 
ataactttt ggtcgagcc aattccgaa tgcattgta 
tattacgtt gacgaaagc acaggtggt ttggaagtg 
tctcacaat tgtgttttg agtagtaaa gattatcct 
ggtgatcct ggagctgta gctcaagta ttgtggact 
ccagatggt tgtgcattg gttgctgca tgggaaaaa 
gggggtatt gcaatgtgg tcaactttt ggatcactt 
ttaatgtgt tctttgggt tgggattat gggttaaat 
atcgatctc cagacaaat aatccctta caaattaaa 
tcaatgcag ttcgcgacg gaaggatat cagttatgg 
atggtacat aaggaggcc aaagatggg gaagttaat 
agtaatggg acatgtacg aatttgttg cagttggat 
tttatgaaa agtgcccta actatcaat ccatgtatg 
agccaccag agccacttg taccttcaa ggcgaggac 
aaactctac gtaaactct gccgacact ttgatcaaa 
atgttttcg gagcgctct accaaagac gagggcgtt 
ttcaacgaa tcgctctca atgccctcc actctggcc 
gaaggcaga caatggctt gtgattccc gttccttcc 
acatactcg gcaacgaat tggccgatt cgatattcg 
gcgatagat tcagacggt caaaacatg gcaatcgcc 
ggtcgcact ggccttgcc cactattcc atgcaaaca 
cgccgatgg aaactattc ggtaacgaa acacaagag 
aaggatttt atcgtagtt gggggtttg ttatggtgg 
cgggattac ctggtaatg ggctgctac agcattttg 
gaaaattcg gacgagtta cggttttat ccaagagac 
gcaaagttg gataataaa tttgcgaaa attgtgact 
gtgttatcg ccgattctc ctcatgaat atcctacag 
gatcagttg attacgttc ggatcggat gcacaagtc 
acgatatgg gctcttaaa cataacccg actgtcgga 
aacgttgaa gtttttaaa gtccaagtc gtggatata 
tcagcgttg gctgtgcat cctgcttgt atcgtttcg 
gtgactttg tcgtctttg cggaccgaa acgggccga 
ggccaaccg aataattcc gaaagtatt gttcttaac 
gtaagtggt agacttttg atggtacaa agagaagtg 
aggaatggg gaacgttat acgtgttct atgccgact 
gttcttgcc agttgtgtg gaaaatgtc tgggtgccc 
agtcggaga aaggccgag aaggctcat ttaaccgaa 
gctttatgg ttgttttgt ggggcgcat ggtatgagg 
gtttggttg cctctctat ccgaaagat ggcgataag 
acgcatact tttatgtcg aagaggatt atgctaccg 
ttccatctt aaaatatat ccgttagca attctcttt 
gaagacgca ataattttg ggagccgag aatgacaca 
gtcctgtat acgtccgat tcaaattcg ccgttttct 
ctaccgttt agtgtatta caacgtact agtcaggtg 
taccttcat caaatctta cgtcaatta atccgaaga 
aacctcggt tatcacgcg tgggaaata gctcgaagc 
tgcatgagt ttgccttat tttccccat tcgttagag 
ctgttgtta cacgaggtg ttggaggag gaggccacc 
agtaaggaa ccaattccc gatgctcaa ttaccaagc 
gtgatagaa tttattatg gaatttccc gtctattta 
cagacggta gtgcaatgt gcccgaaag actgaaatc 
gctctatgg ccgtatctt ttttcggcc gccggcaaa 
ccgaaagat ctttttcaa gaatgcatg gccaagaga 
caactcgat acggccgct tcctacctc attattttg 
caaaatttg gaaacttcc tctgttagt agacagtac 
gccacgttg ttgctgaat acggccctg gatcagtcc 
aagtgggag ctcgccaag gatctagtt cgcttcctg 
agagccatc gacccaaac gacgttgaa tctcctcgg 
acgtccttc atcctgccc cccaagctg ggaatctcc 
caacaaaca ccgcccgtg agccccaac gctgaagac 
ctctcccta attctgggc aatgtgcaa ggcccccgt 
gtccgcagc tacagcacg acaatatcc cccaaacta 
gtcgaaaac aggcccaat aacccgacc aaccatccg 
attgaatca ccacaaaca gcccggaaa aaatccgta 
ccaaacaca gttggtcgc aactccccg aataacaac 
acagccgag gagtttttc atcgatgtt attttacaa 
aaacacgct cgcaggtta ctttcaacc cgccgttta 
accgatctt ggctacttt gccgcgcat ttggatttc 
catctggtg gcttggttg ggcaaagag cgcgatcgt 
gctgcgaga attgacgat ttcgtccag gccttgaag 
cacctccat gaggagttc caatggcct tatccaacc 
ccaagtgcg ttctctcca ccaaacact ggatgcgac 
cttgaagac cgtctcaaa ttactaaaa atcgacgta 
acgcatcca gcgtccaga gtgggggat agcggttat 
atgagtttt caaggggtg ccttgcacg gggctaatc 
accccgatt aacaccctg gactcggag acggagctc 
gatgatttg tcgcaaagc gagacgaat aaaatcgac 
gataagggt gatttagcc acgagttcg actttttac 
gataagagg attgatttg gctcaggtt ggtggaagg 
gttgatgat gcggcaatg agttcggtt agttcggtc 
tggggtgag gataggatt gagtcgagt aatgataat 
tgggaggtg ccctcaaca caaattttg gaacagttg 
tccaatacg gataataaa gggtcacag aggtctgaa 
gttcagttg aggtatttg ctgcaattg tttatggaa 
gcgagttgt ttggaatgg tcgttgatt atttcggtg 
cttttgagg gatgcgatg gctgttttg aggacagtg 
aatgcggcc aaaagtcca gatcagtcg attgaggcg 
gtgtcgagg ctgaggcaa gggttgctg ctgctgcag 
tattggaca aacactgaa tgtttgggc tacaaagcc 
ttcatgttg gcaatacaa aatcaaata tccgtcctt 
tcgaaaatc ctcgagaca aaaatccaa catcagaag 
atagtggag taccagcag gcagtgtct gcgtcactc 
aataaactc tcaacgcca attccggcc cgatctcgt 
aaaaccagc tcgaatcac gataattct agtcaaagt 
ggttcggtc aaagaaaag aaaaacatc aacataacc 
accagtcac agcacaacc gacaaactc aacgaaatg 
aacccgata aattccagt agctgcgcc aaaacaata 
aatcaggag aataataat caagtggaa gatgcaatc 
attccggaa agcaccggc tgcgttata tcatag
    mRNA sequence
    • >TC009376 RA
      tcagttgac gtttgtgcg aagtgtcaa acgaagcca 
caaaaactt tcccaaaaa agaatacaa taataatga 
agtgttcaa tttgttaac ggcaacaat cagcttaaa 
atgtgacta gttatgata gtgtggtgt tgaagctaa 
atccaaaat gtacttccc gatcggttg gccaaaagt 
catcaaaat accagattt aggacatgc aagtgtaag 
gcaaataac ttgcaacag ggacaggat actgtttgc 
tatactaac ggacgattc gatcgcgat ttggttttg 
taaaccatg cgttccaat tgtcttcta tcgaagaac 
cccacagtc cttggaaaa attcggaac gaacatttt 
aaccgaatg gaagccgga ctccagcat ggtggcagt 
cgcgacgtc agaaggcca cttgttatt gtttaaact 
aggagtgat agctgataa taaaggttt atacgtcca 
gactgatag cccccatgc gaatttacg tagggacag 
cgccgaatt gttcattaa agaaataat tccgcctct 
gcatctcac tctgcacca agaaattat ggtgtggga 
cggtaaaat aacaggaat cgtctgtat aacaatgtc 
agagtttat gatctcaac gagtgaagg acatgtctt 
gaggtattg gtgggatgg acaacaaca tagagacta 
taatttgga tttgaggag aataccttt ctgtatcaa 
tcaacaagt gtccaaagc aatcccaat tgttgaaga 
aaatacgtt tatagttga tatagaata ttcgccact 
agttggcgg tttttcaat tgttttaaa tgacggaag 
agcggcgtt tttaacagc ctcctctct caaatttga 
tcccaatca agtccaagg aatatgggc ccagaacat 
cgaagacgc aacttgcac cgttatgaa ccacaaata 
ccgattaat aacttttgg tcgagccaa ttccgaatg 
cattgtata ttacgttga cgaaagcac aggtggttt 
ggaagtgtc tcacaattg tgttttgag tagtaaaga 
ttatcctgg tgatcctgg agctgtagc tcaagtatt 
gtggactcc agatggttg tgcattggt tgctgcatg 
ggaaaaagg gggtattgc aatgtggtc aacttttgg 
atcactttt aatgtgttc tttgggttg ggattatgg 
gttaaatat cgatctcca gacaaataa tcccttaca 
aattaaatc aatgcagtt cgcgacgga aggatatca 
gttatggat ggtacataa ggaggccaa agatgggga 
agttaatag taatgggac atgtacgaa tttgttgca 
gttggattt tatgaaaag tgccctaac tatcaatcc 
atgtatgag ccaccagag ccacttgta ccttcaagg 
cgaggacaa actctacgt aaactctgc cgacacttt 
gatcaaaat gttttcgga gcgctctac caaagacga 
gggcgtttt caacgaatc gctctcaat gccctccac 
tctggccga aggcagaca atggcttgt gattcccgt 
tccttccac atactcggc aacgaattg gccgattcg 
atattcggc gatagattc agacggtca aaacatggc 
aatcgccgg tcgcactgg ccttgccca ctattccat 
gcaaacacg ccgatggaa actattcgg taacgaaac 
acaagagaa ggattttat cgtagttgg gggtttgtt 
atggtggcg ggattacct ggtaatggg ctgctacag 
cattttgga aaattcgga cgagttacg gttttatcc 
aagagacgc aaagttgga taataaatt tgcgaaaat 
tgtgactgt gttatcgcc gattctcct catgaatat 
cctacagga tcagttgat tacgttcgg atcggatgc 
acaagtcac gatatgggc tcttaaaca taacccgac 
tgtcggaaa cgttgaagt ttttaaagt ccaagtcgt 
ggatatatc agcgttggc tgtgcatcc tgcttgtat 
cgtttcggt gactttgtc gtctttgcg gaccgaaac 
gggccgagg ccaaccgaa taattccga aagtattgt 
tcttaacgt aagtggtag acttttgat ggtacaaag 
agaagtgag gaatgggga acgttatac gtgttctat 
gccgactgt tcttgccag ttgtgtgga aaatgtctg 
ggtgcccag tcggagaaa ggccgagaa ggctcattt 
aaccgaagc tttatggtt gttttgtgg ggcgcatgg 
tatgagggt ttggttgcc tctctatcc gaaagatgg 
cgataagac gcatacttt tatgtcgaa gaggattat 
gctaccgtt ccatcttaa aatatatcc gttagcaat 
tctctttga agacgcaat aattttggg agccgagaa 
tgacacagt cctgtatac gtccgattc aaattcgcc 
gttttctct accgtttag tgtattaca acgtactag 
tcaggtgta ccttcatca aatcttacg tcaattaat 
ccgaagaaa cctcggtta tcacgcgtg ggaaatagc 
tcgaagctg catgagttt gccttattt tccccattc 
gttagagct gttgttaca cgaggtgtt ggaggagga 
ggccaccag taaggaacc aattcccga tgctcaatt 
accaagcgt gatagaatt tattatgga atttcccgt 
ctatttaca gacggtagt gcaatgtgc ccgaaagac 
tgaaatcgc tctatggcc gtatctttt ttcggccgc 
cggcaaacc gaaagatct ttttcaaga atgcatggc 
caagagaca actcgatac ggccgcttc ctacctcat 
tattttgca aaatttgga aacttcctc tgttagtag 
acagtacgc cacgttgtt gctgaatac ggccctgga 
tcagtccaa gtgggagct cgccaagga tctagttcg 
cttcctgag agccatcga cccaaacga cgttgaatc 
tcctcggac gtccttcat cctgccccc caagctggg 
aatctccca acaaacacc gcccgtgag ccccaacgc 
tgaagacct ctccctaat tctgggcaa tgtgcaagg 
cccccgtgt ccgcagcta cagcacgac aatatcccc 
caaactagt cgaaaacag gcccaataa cccgaccaa 
ccatccgat tgaatcacc acaaacagc ccggaaaaa 
atccgtacc aaacacagt tggtcgcaa ctccccgaa 
taacaacac agccgagga gtttttcat cgatgttat 
tttacaaaa acacgctcg caggttact ttcaacccg 
ccgtttaac cgatcttgg ctactttgc cgcgcattt 
ggatttcca tctggtggc ttggttggg caaagagcg 
cgatcgtgc tgcgagaat tgacgattt cgtccaggc 
cttgaagca cctccatga ggagttcca atggcctta 
tccaacccc aagtgcgtt ctctccacc aaacactgg 
atgcgacct tgaagaccg tctcaaatt actaaaaat 
cgacgtaac gcatccagc gtccagagt gggggatag 
cggttatat gagttttca aggggtgcc ttgcacggg 
gctaatcac cccgattaa caccctgga ctcggagac 
ggagctcga tgatttgtc gcaaagcga gacgaataa 
aatcgacga taagggtga tttagccac gagttcgac 
tttttacga taagaggat tgatttggc tcaggttgg 
tggaagggt tgatgatgc ggcaatgag ttcggttag 
ttcggtctg gggtgagga taggattga gtcgagtaa 
tgataattg ggaggtgcc ctcaacaca aattttgga 
acagttgtc caatacgga taataaagg gtcacagag 
gtctgaagt tcagttgag gtatttgct gcaattgtt 
tatggaagc gagttgttt ggaatggtc gttgattat 
ttcggtgct tttgaggga tgcgatggc tgttttgag 
gacagtgaa tgcggccaa aagtccaga tcagtcgat 
tgaggcggt gtcgaggct gaggcaagg gttgctgct 
gctgcagta ttggacaaa cactgaatg tttgggcta 
caaagcctt catgttggc aatacaaaa tcaaatatc 
cgtcctttc gaaaatcct cgagacaaa aatccaaca 
tcagaagat agtggagta ccagcaggc agtgtctgc 
gtcactcaa taaactctc aacgccaat tccggcccg 
atctcgtaa aaccagctc gaatcacga taattctag 
tcaaagtgg ttcggtcaa agaaaagaa aaacatcaa 
cataaccac cagtcacag cacaaccga caaactcaa 
cgaaatgaa cccgataaa ttccagtag ctgcgccaa 
aacaataaa tcaggagaa taataatca agtggaaga 
tgcaatcat tccggaaag caccggctg cgttatatc 
ataggtttt ttacgaaat aaatttttg ggtgtacta 
tttgtggac actcggaac tgcctaaaa atgcgatct 
aatccgtct tagaagtga taggtacgt aacaaatgt 
tgttgcact tttttgttt tacgtaaaa atgaaaccg 
ttttcaatt gtgttgtgc ctcgcattt atgaaacgt 
cgtgcattc cgctgaaag aaatatttt tgcaaacga 
tattaccgt atgagtttt tcagcaata acactagtt 
attcgtagc aattcggtc cgaacaatc gacttgggt 
gataaggtg ttgaattat taatgttgt aggttttaa 
aatctgttt ctaataata ttgttggtc aatttttat 
acaataatc gtagactgt cgtcttatt aatactcca 
ctatattgt tgtataatt tattacttt tttcaacag 
ttttatttt gtaacatac taaaaataa ataacagtt 
ttattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY