| General information | |||
| TC009376 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1172776..1181865 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009376 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04623 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 6 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (19 dpi)
adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
wings not present - Number of animals showing the phenotype: 1
- >TC009376 RA
MYFPIGWPK VIKIPDLGH ASVRQITCN RDRILFAIL TDDSIAIWF CKPCVPIVF YRRTPQSLE KFGTNILTE WKPDSSMVA VATSEGHLL LFKLGVIAD NKGLYVQTD SPHANLRRD SAELFIKEI IPPLHLTLH QEIMVWDGK ITGIVCITM SEFMISTSE GHVLRYWWD GQQHRDYNL DLRRIPFCI NQQVSKAIP IVEENTFIV DIEYSPLVG GFSIVLNDG RAAFLTASS LKFDPNQVQ GIWAQNIED ATCTVMNHK YRLITFGRA NSECIVYYV DESTGGLEV SHNCVLSSK DYPGDPGAV AQVLWTPDG CALVAAWEK GGIAMWSTF GSLLMCSLG WDYGLNIDL QTNNPLQIK SMQFATEGY QLWMVHKEA KDGEVNSNG TCTNLLQLD FMKSALTIN PCMSHQSHL YLQGEDKLY VNSADTLIK MFSERSTKD EGVFNESLS MPSTLAEGR QWLVIPVPS TYSATNWPI RYSAIDSDG QNMAIAGRT GLAHYSMQT RRWKLFGNE TQEKDFIVV GGLLWWRDY LVMGCYSIL ENSDELRFY PRDAKLDNK FAKIVTVLS PILLMNILQ DQLITFGSD AQVTIWALK HNPTVGNVE VFKVQVVDI SALAVHPAC IVSVTLSSL RTETGRGQP NNSESIVLN VSGRLLMVQ REVRNGERY TCSMPTVLA SCVENVWVP SRRKAEKAH LTEALWLFC GAHGMRVWL PLYPKDGDK THTFMSKRI MLPFHLKIY PLAILFEDA IILGAENDT VLYTSDSNS PFSLPFSVL QRTSQVYLH QILRQLIRR NLGYHAWEI ARSCMSLPY FPHSLELLL HEVLEEEAT SKEPIPDAQ LPSVIEFIM EFPVYLQTV VQCARKTEI ALWPYLFSA AGKPKDLFQ ECMAKRQLD TAASYLIIL QNLETSSVS RQYATLLLN TALDQSKWE LAKDLVRFL RAIDPNDVE SPRTSFILP PKLGISQQT PPVSPNAED LSLILGNVQ GPRVRSYST TISPKLVEN RPNNPTNHP IESPQTARK KSVPNTVGR NSPNNNTAE EFFIDVILQ KHARRLLST RRLTDLGYF AAHLDFHLV AWLGKERDR AARIDDFVQ ALKHLHEEF QWPYPTPSA FSPPNTGCD LEDRLKLLK IDVTHPASR VGDSGYMSF QGVPCTGLI TPINTLDSE TELDDLSQS ETNKIDDKG DLATSSTFY DKRIDLAQV GGRVDDAAM SSVSSVWGE DRIESSNDN WEVPSTQIL EQLSNTDNK GSQRSEVQL RYLLQLFME ASCLEWSLI ISVLLRDAM AVLRTVNAA KSPDQSIEA VSRLRQGLL LLQYWTNTE CLGYKAFML AIQNQISVL SKILETKIQ HQKIVEYQQ AVSASLNKL STPIPARSR KTSSNHDNS SQSGSVKEK KNINITTSH STTDKLNEM NPINSSSCA KTINQENNN QVEDAIIPE STGCVIS
- >TC009376 RA
atgtacttc ccgatcggt tggccaaaa gtcatcaaa ataccagat ttaggacat gcaagtgta aggcaaata acttgcaac agggacagg atactgttt gctatacta acggacgat tcgatcgcg atttggttt tgtaaacca tgcgttcca attgtcttc tatcgaaga accccacag tccttggaa aaattcgga acgaacatt ttaaccgaa tggaagccg gactccagc atggtggca gtcgcgacg tcagaaggc cacttgtta ttgtttaaa ctaggagtg atagctgat aataaaggt ttatacgtc cagactgat agcccccat gcgaattta cgtagggac agcgccgaa ttgttcatt aaagaaata attccgcct ctgcatctc actctgcac caagaaatt atggtgtgg gacggtaaa ataacagga atcgtctgt ataacaatg tcagagttt atgatctca acgagtgaa ggacatgtc ttgaggtat tggtgggat ggacaacaa catagagac tataatttg gatttgagg agaatacct ttctgtatc aatcaacaa gtgtccaaa gcaatccca attgttgaa gaaaatacg tttatagtt gatatagaa tattcgcca ctagttggc ggtttttca attgtttta aatgacgga agagcggcg tttttaaca gcctcctct ctcaaattt gatcccaat caagtccaa ggaatatgg gcccagaac atcgaagac gcaacttgc accgttatg aaccacaaa taccgatta ataactttt ggtcgagcc aattccgaa tgcattgta tattacgtt gacgaaagc acaggtggt ttggaagtg tctcacaat tgtgttttg agtagtaaa gattatcct ggtgatcct ggagctgta gctcaagta ttgtggact ccagatggt tgtgcattg gttgctgca tgggaaaaa gggggtatt gcaatgtgg tcaactttt ggatcactt ttaatgtgt tctttgggt tgggattat gggttaaat atcgatctc cagacaaat aatccctta caaattaaa tcaatgcag ttcgcgacg gaaggatat cagttatgg atggtacat aaggaggcc aaagatggg gaagttaat agtaatggg acatgtacg aatttgttg cagttggat tttatgaaa agtgcccta actatcaat ccatgtatg agccaccag agccacttg taccttcaa ggcgaggac aaactctac gtaaactct gccgacact ttgatcaaa atgttttcg gagcgctct accaaagac gagggcgtt ttcaacgaa tcgctctca atgccctcc actctggcc gaaggcaga caatggctt gtgattccc gttccttcc acatactcg gcaacgaat tggccgatt cgatattcg gcgatagat tcagacggt caaaacatg gcaatcgcc ggtcgcact ggccttgcc cactattcc atgcaaaca cgccgatgg aaactattc ggtaacgaa acacaagag aaggatttt atcgtagtt gggggtttg ttatggtgg cgggattac ctggtaatg ggctgctac agcattttg gaaaattcg gacgagtta cggttttat ccaagagac gcaaagttg gataataaa tttgcgaaa attgtgact gtgttatcg ccgattctc ctcatgaat atcctacag gatcagttg attacgttc ggatcggat gcacaagtc acgatatgg gctcttaaa cataacccg actgtcgga aacgttgaa gtttttaaa gtccaagtc gtggatata tcagcgttg gctgtgcat cctgcttgt atcgtttcg gtgactttg tcgtctttg cggaccgaa acgggccga ggccaaccg aataattcc gaaagtatt gttcttaac gtaagtggt agacttttg atggtacaa agagaagtg aggaatggg gaacgttat acgtgttct atgccgact gttcttgcc agttgtgtg gaaaatgtc tgggtgccc agtcggaga aaggccgag aaggctcat ttaaccgaa gctttatgg ttgttttgt ggggcgcat ggtatgagg gtttggttg cctctctat ccgaaagat ggcgataag acgcatact tttatgtcg aagaggatt atgctaccg ttccatctt aaaatatat ccgttagca attctcttt gaagacgca ataattttg ggagccgag aatgacaca gtcctgtat acgtccgat tcaaattcg ccgttttct ctaccgttt agtgtatta caacgtact agtcaggtg taccttcat caaatctta cgtcaatta atccgaaga aacctcggt tatcacgcg tgggaaata gctcgaagc tgcatgagt ttgccttat tttccccat tcgttagag ctgttgtta cacgaggtg ttggaggag gaggccacc agtaaggaa ccaattccc gatgctcaa ttaccaagc gtgatagaa tttattatg gaatttccc gtctattta cagacggta gtgcaatgt gcccgaaag actgaaatc gctctatgg ccgtatctt ttttcggcc gccggcaaa ccgaaagat ctttttcaa gaatgcatg gccaagaga caactcgat acggccgct tcctacctc attattttg caaaatttg gaaacttcc tctgttagt agacagtac gccacgttg ttgctgaat acggccctg gatcagtcc aagtgggag ctcgccaag gatctagtt cgcttcctg agagccatc gacccaaac gacgttgaa tctcctcgg acgtccttc atcctgccc cccaagctg ggaatctcc caacaaaca ccgcccgtg agccccaac gctgaagac ctctcccta attctgggc aatgtgcaa ggcccccgt gtccgcagc tacagcacg acaatatcc cccaaacta gtcgaaaac aggcccaat aacccgacc aaccatccg attgaatca ccacaaaca gcccggaaa aaatccgta ccaaacaca gttggtcgc aactccccg aataacaac acagccgag gagtttttc atcgatgtt attttacaa aaacacgct cgcaggtta ctttcaacc cgccgttta accgatctt ggctacttt gccgcgcat ttggatttc catctggtg gcttggttg ggcaaagag cgcgatcgt gctgcgaga attgacgat ttcgtccag gccttgaag cacctccat gaggagttc caatggcct tatccaacc ccaagtgcg ttctctcca ccaaacact ggatgcgac cttgaagac cgtctcaaa ttactaaaa atcgacgta acgcatcca gcgtccaga gtgggggat agcggttat atgagtttt caaggggtg ccttgcacg gggctaatc accccgatt aacaccctg gactcggag acggagctc gatgatttg tcgcaaagc gagacgaat aaaatcgac gataagggt gatttagcc acgagttcg actttttac gataagagg attgatttg gctcaggtt ggtggaagg gttgatgat gcggcaatg agttcggtt agttcggtc tggggtgag gataggatt gagtcgagt aatgataat tgggaggtg ccctcaaca caaattttg gaacagttg tccaatacg gataataaa gggtcacag aggtctgaa gttcagttg aggtatttg ctgcaattg tttatggaa gcgagttgt ttggaatgg tcgttgatt atttcggtg cttttgagg gatgcgatg gctgttttg aggacagtg aatgcggcc aaaagtcca gatcagtcg attgaggcg gtgtcgagg ctgaggcaa gggttgctg ctgctgcag tattggaca aacactgaa tgtttgggc tacaaagcc ttcatgttg gcaatacaa aatcaaata tccgtcctt tcgaaaatc ctcgagaca aaaatccaa catcagaag atagtggag taccagcag gcagtgtct gcgtcactc aataaactc tcaacgcca attccggcc cgatctcgt aaaaccagc tcgaatcac gataattct agtcaaagt ggttcggtc aaagaaaag aaaaacatc aacataacc accagtcac agcacaacc gacaaactc aacgaaatg aacccgata aattccagt agctgcgcc aaaacaata aatcaggag aataataat caagtggaa gatgcaatc attccggaa agcaccggc tgcgttata tcatag
- >TC009376 RA
tcagttgac gtttgtgcg aagtgtcaa acgaagcca caaaaactt tcccaaaaa agaatacaa taataatga agtgttcaa tttgttaac ggcaacaat cagcttaaa atgtgacta gttatgata gtgtggtgt tgaagctaa atccaaaat gtacttccc gatcggttg gccaaaagt catcaaaat accagattt aggacatgc aagtgtaag gcaaataac ttgcaacag ggacaggat actgtttgc tatactaac ggacgattc gatcgcgat ttggttttg taaaccatg cgttccaat tgtcttcta tcgaagaac cccacagtc cttggaaaa attcggaac gaacatttt aaccgaatg gaagccgga ctccagcat ggtggcagt cgcgacgtc agaaggcca cttgttatt gtttaaact aggagtgat agctgataa taaaggttt atacgtcca gactgatag cccccatgc gaatttacg tagggacag cgccgaatt gttcattaa agaaataat tccgcctct gcatctcac tctgcacca agaaattat ggtgtggga cggtaaaat aacaggaat cgtctgtat aacaatgtc agagtttat gatctcaac gagtgaagg acatgtctt gaggtattg gtgggatgg acaacaaca tagagacta taatttgga tttgaggag aataccttt ctgtatcaa tcaacaagt gtccaaagc aatcccaat tgttgaaga aaatacgtt tatagttga tatagaata ttcgccact agttggcgg tttttcaat tgttttaaa tgacggaag agcggcgtt tttaacagc ctcctctct caaatttga tcccaatca agtccaagg aatatgggc ccagaacat cgaagacgc aacttgcac cgttatgaa ccacaaata ccgattaat aacttttgg tcgagccaa ttccgaatg cattgtata ttacgttga cgaaagcac aggtggttt ggaagtgtc tcacaattg tgttttgag tagtaaaga ttatcctgg tgatcctgg agctgtagc tcaagtatt gtggactcc agatggttg tgcattggt tgctgcatg ggaaaaagg gggtattgc aatgtggtc aacttttgg atcactttt aatgtgttc tttgggttg ggattatgg gttaaatat cgatctcca gacaaataa tcccttaca aattaaatc aatgcagtt cgcgacgga aggatatca gttatggat ggtacataa ggaggccaa agatgggga agttaatag taatgggac atgtacgaa tttgttgca gttggattt tatgaaaag tgccctaac tatcaatcc atgtatgag ccaccagag ccacttgta ccttcaagg cgaggacaa actctacgt aaactctgc cgacacttt gatcaaaat gttttcgga gcgctctac caaagacga gggcgtttt caacgaatc gctctcaat gccctccac tctggccga aggcagaca atggcttgt gattcccgt tccttccac atactcggc aacgaattg gccgattcg atattcggc gatagattc agacggtca aaacatggc aatcgccgg tcgcactgg ccttgccca ctattccat gcaaacacg ccgatggaa actattcgg taacgaaac acaagagaa ggattttat cgtagttgg gggtttgtt atggtggcg ggattacct ggtaatggg ctgctacag cattttgga aaattcgga cgagttacg gttttatcc aagagacgc aaagttgga taataaatt tgcgaaaat tgtgactgt gttatcgcc gattctcct catgaatat cctacagga tcagttgat tacgttcgg atcggatgc acaagtcac gatatgggc tcttaaaca taacccgac tgtcggaaa cgttgaagt ttttaaagt ccaagtcgt ggatatatc agcgttggc tgtgcatcc tgcttgtat cgtttcggt gactttgtc gtctttgcg gaccgaaac gggccgagg ccaaccgaa taattccga aagtattgt tcttaacgt aagtggtag acttttgat ggtacaaag agaagtgag gaatgggga acgttatac gtgttctat gccgactgt tcttgccag ttgtgtgga aaatgtctg ggtgcccag tcggagaaa ggccgagaa ggctcattt aaccgaagc tttatggtt gttttgtgg ggcgcatgg tatgagggt ttggttgcc tctctatcc gaaagatgg cgataagac gcatacttt tatgtcgaa gaggattat gctaccgtt ccatcttaa aatatatcc gttagcaat tctctttga agacgcaat aattttggg agccgagaa tgacacagt cctgtatac gtccgattc aaattcgcc gttttctct accgtttag tgtattaca acgtactag tcaggtgta ccttcatca aatcttacg tcaattaat ccgaagaaa cctcggtta tcacgcgtg ggaaatagc tcgaagctg catgagttt gccttattt tccccattc gttagagct gttgttaca cgaggtgtt ggaggagga ggccaccag taaggaacc aattcccga tgctcaatt accaagcgt gatagaatt tattatgga atttcccgt ctatttaca gacggtagt gcaatgtgc ccgaaagac tgaaatcgc tctatggcc gtatctttt ttcggccgc cggcaaacc gaaagatct ttttcaaga atgcatggc caagagaca actcgatac ggccgcttc ctacctcat tattttgca aaatttgga aacttcctc tgttagtag acagtacgc cacgttgtt gctgaatac ggccctgga tcagtccaa gtgggagct cgccaagga tctagttcg cttcctgag agccatcga cccaaacga cgttgaatc tcctcggac gtccttcat cctgccccc caagctggg aatctccca acaaacacc gcccgtgag ccccaacgc tgaagacct ctccctaat tctgggcaa tgtgcaagg cccccgtgt ccgcagcta cagcacgac aatatcccc caaactagt cgaaaacag gcccaataa cccgaccaa ccatccgat tgaatcacc acaaacagc ccggaaaaa atccgtacc aaacacagt tggtcgcaa ctccccgaa taacaacac agccgagga gtttttcat cgatgttat tttacaaaa acacgctcg caggttact ttcaacccg ccgtttaac cgatcttgg ctactttgc cgcgcattt ggatttcca tctggtggc ttggttggg caaagagcg cgatcgtgc tgcgagaat tgacgattt cgtccaggc cttgaagca cctccatga ggagttcca atggcctta tccaacccc aagtgcgtt ctctccacc aaacactgg atgcgacct tgaagaccg tctcaaatt actaaaaat cgacgtaac gcatccagc gtccagagt gggggatag cggttatat gagttttca aggggtgcc ttgcacggg gctaatcac cccgattaa caccctgga ctcggagac ggagctcga tgatttgtc gcaaagcga gacgaataa aatcgacga taagggtga tttagccac gagttcgac tttttacga taagaggat tgatttggc tcaggttgg tggaagggt tgatgatgc ggcaatgag ttcggttag ttcggtctg gggtgagga taggattga gtcgagtaa tgataattg ggaggtgcc ctcaacaca aattttgga acagttgtc caatacgga taataaagg gtcacagag gtctgaagt tcagttgag gtatttgct gcaattgtt tatggaagc gagttgttt ggaatggtc gttgattat ttcggtgct tttgaggga tgcgatggc tgttttgag gacagtgaa tgcggccaa aagtccaga tcagtcgat tgaggcggt gtcgaggct gaggcaagg gttgctgct gctgcagta ttggacaaa cactgaatg tttgggcta caaagcctt catgttggc aatacaaaa tcaaatatc cgtcctttc gaaaatcct cgagacaaa aatccaaca tcagaagat agtggagta ccagcaggc agtgtctgc gtcactcaa taaactctc aacgccaat tccggcccg atctcgtaa aaccagctc gaatcacga taattctag tcaaagtgg ttcggtcaa agaaaagaa aaacatcaa cataaccac cagtcacag cacaaccga caaactcaa cgaaatgaa cccgataaa ttccagtag ctgcgccaa aacaataaa tcaggagaa taataatca agtggaaga tgcaatcat tccggaaag caccggctg cgttatatc ataggtttt ttacgaaat aaatttttg ggtgtacta tttgtggac actcggaac tgcctaaaa atgcgatct aatccgtct tagaagtga taggtacgt aacaaatgt tgttgcact tttttgttt tacgtaaaa atgaaaccg ttttcaatt gtgttgtgc ctcgcattt atgaaacgt cgtgcattc cgctgaaag aaatatttt tgcaaacga tattaccgt atgagtttt tcagcaata acactagtt attcgtagc aattcggtc cgaacaatc gacttgggt gataaggtg ttgaattat taatgttgt aggttttaa aatctgttt ctaataata ttgttggtc aatttttat acaataatc gtagactgt cgtcttatt aatactcca ctatattgt tgtataatt tattacttt tttcaacag ttttatttt gtaacatac taaaaataa ataacagtt ttattc
