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Gene information
General information
TC009376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1172776..1181865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009376
    iBeetle screen iB_04623

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    labrum shortened - Number of animals showing the phenotype: 4

    elytra not closed - Number of animals showing the phenotype: 4

    Adult morphology
    prosternal process broader - Number of animals showing the phenotype: 2


    wings not present - Number of animals showing the phenotype: 1


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009376 RA
      MYFPIGWPK VIKIPDLGH ASVRQITCN RDRILFAIL 
      TDDSIAIWF CKPCVPIVF YRRTPQSLE KFGTNILTE 
      WKPDSSMVA VATSEGHLL LFKLGVIAD NKGLYVQTD 
      SPHANLRRD SAELFIKEI IPPLHLTLH QEIMVWDGK 
      ITGIVCITM SEFMISTSE GHVLRYWWD GQQHRDYNL 
      DLRRIPFCI NQQVSKAIP IVEENTFIV DIEYSPLVG 
      GFSIVLNDG RAAFLTASS LKFDPNQVQ GIWAQNIED 
      ATCTVMNHK YRLITFGRA NSECIVYYV DESTGGLEV 
      SHNCVLSSK DYPGDPGAV AQVLWTPDG CALVAAWEK 
      GGIAMWSTF GSLLMCSLG WDYGLNIDL QTNNPLQIK 
      SMQFATEGY QLWMVHKEA KDGEVNSNG TCTNLLQLD 
      FMKSALTIN PCMSHQSHL YLQGEDKLY VNSADTLIK 
      MFSERSTKD EGVFNESLS MPSTLAEGR QWLVIPVPS 
      TYSATNWPI RYSAIDSDG QNMAIAGRT GLAHYSMQT 
      RRWKLFGNE TQEKDFIVV GGLLWWRDY LVMGCYSIL 
      ENSDELRFY PRDAKLDNK FAKIVTVLS PILLMNILQ 
      DQLITFGSD AQVTIWALK HNPTVGNVE VFKVQVVDI 
      SALAVHPAC IVSVTLSSL RTETGRGQP NNSESIVLN 
      VSGRLLMVQ REVRNGERY TCSMPTVLA SCVENVWVP 
      SRRKAEKAH LTEALWLFC GAHGMRVWL PLYPKDGDK 
      THTFMSKRI MLPFHLKIY PLAILFEDA IILGAENDT 
      VLYTSDSNS PFSLPFSVL QRTSQVYLH QILRQLIRR 
      NLGYHAWEI ARSCMSLPY FPHSLELLL HEVLEEEAT 
      SKEPIPDAQ LPSVIEFIM EFPVYLQTV VQCARKTEI 
      ALWPYLFSA AGKPKDLFQ ECMAKRQLD TAASYLIIL 
      QNLETSSVS RQYATLLLN TALDQSKWE LAKDLVRFL 
      RAIDPNDVE SPRTSFILP PKLGISQQT PPVSPNAED 
      LSLILGNVQ GPRVRSYST TISPKLVEN RPNNPTNHP 
      IESPQTARK KSVPNTVGR NSPNNNTAE EFFIDVILQ 
      KHARRLLST RRLTDLGYF AAHLDFHLV AWLGKERDR 
      AARIDDFVQ ALKHLHEEF QWPYPTPSA FSPPNTGCD 
      LEDRLKLLK IDVTHPASR VGDSGYMSF QGVPCTGLI 
      TPINTLDSE TELDDLSQS ETNKIDDKG DLATSSTFY 
      DKRIDLAQV GGRVDDAAM SSVSSVWGE DRIESSNDN 
      WEVPSTQIL EQLSNTDNK GSQRSEVQL RYLLQLFME 
      ASCLEWSLI ISVLLRDAM AVLRTVNAA KSPDQSIEA 
      VSRLRQGLL LLQYWTNTE CLGYKAFML AIQNQISVL 
      SKILETKIQ HQKIVEYQQ AVSASLNKL STPIPARSR 
      KTSSNHDNS SQSGSVKEK KNINITTSH STTDKLNEM 
      NPINSSSCA KTINQENNN QVEDAIIPE STGCVIS
    CDS sequence
    • >TC009376 RA
      atgtacttc ccgatcggt tggccaaaa gtcatcaaa 
      ataccagat ttaggacat gcaagtgta aggcaaata 
      acttgcaac agggacagg atactgttt gctatacta 
      acggacgat tcgatcgcg atttggttt tgtaaacca 
      tgcgttcca attgtcttc tatcgaaga accccacag 
      tccttggaa aaattcgga acgaacatt ttaaccgaa 
      tggaagccg gactccagc atggtggca gtcgcgacg 
      tcagaaggc cacttgtta ttgtttaaa ctaggagtg 
      atagctgat aataaaggt ttatacgtc cagactgat 
      agcccccat gcgaattta cgtagggac agcgccgaa 
      ttgttcatt aaagaaata attccgcct ctgcatctc 
      actctgcac caagaaatt atggtgtgg gacggtaaa 
      ataacagga atcgtctgt ataacaatg tcagagttt 
      atgatctca acgagtgaa ggacatgtc ttgaggtat 
      tggtgggat ggacaacaa catagagac tataatttg 
      gatttgagg agaatacct ttctgtatc aatcaacaa 
      gtgtccaaa gcaatccca attgttgaa gaaaatacg 
      tttatagtt gatatagaa tattcgcca ctagttggc 
      ggtttttca attgtttta aatgacgga agagcggcg 
      tttttaaca gcctcctct ctcaaattt gatcccaat 
      caagtccaa ggaatatgg gcccagaac atcgaagac 
      gcaacttgc accgttatg aaccacaaa taccgatta 
      ataactttt ggtcgagcc aattccgaa tgcattgta 
      tattacgtt gacgaaagc acaggtggt ttggaagtg 
      tctcacaat tgtgttttg agtagtaaa gattatcct 
      ggtgatcct ggagctgta gctcaagta ttgtggact 
      ccagatggt tgtgcattg gttgctgca tgggaaaaa 
      gggggtatt gcaatgtgg tcaactttt ggatcactt 
      ttaatgtgt tctttgggt tgggattat gggttaaat 
      atcgatctc cagacaaat aatccctta caaattaaa 
      tcaatgcag ttcgcgacg gaaggatat cagttatgg 
      atggtacat aaggaggcc aaagatggg gaagttaat 
      agtaatggg acatgtacg aatttgttg cagttggat 
      tttatgaaa agtgcccta actatcaat ccatgtatg 
      agccaccag agccacttg taccttcaa ggcgaggac 
      aaactctac gtaaactct gccgacact ttgatcaaa 
      atgttttcg gagcgctct accaaagac gagggcgtt 
      ttcaacgaa tcgctctca atgccctcc actctggcc 
      gaaggcaga caatggctt gtgattccc gttccttcc 
      acatactcg gcaacgaat tggccgatt cgatattcg 
      gcgatagat tcagacggt caaaacatg gcaatcgcc 
      ggtcgcact ggccttgcc cactattcc atgcaaaca 
      cgccgatgg aaactattc ggtaacgaa acacaagag 
      aaggatttt atcgtagtt gggggtttg ttatggtgg 
      cgggattac ctggtaatg ggctgctac agcattttg 
      gaaaattcg gacgagtta cggttttat ccaagagac 
      gcaaagttg gataataaa tttgcgaaa attgtgact 
      gtgttatcg ccgattctc ctcatgaat atcctacag 
      gatcagttg attacgttc ggatcggat gcacaagtc 
      acgatatgg gctcttaaa cataacccg actgtcgga 
      aacgttgaa gtttttaaa gtccaagtc gtggatata 
      tcagcgttg gctgtgcat cctgcttgt atcgtttcg 
      gtgactttg tcgtctttg cggaccgaa acgggccga 
      ggccaaccg aataattcc gaaagtatt gttcttaac 
      gtaagtggt agacttttg atggtacaa agagaagtg 
      aggaatggg gaacgttat acgtgttct atgccgact 
      gttcttgcc agttgtgtg gaaaatgtc tgggtgccc 
      agtcggaga aaggccgag aaggctcat ttaaccgaa 
      gctttatgg ttgttttgt ggggcgcat ggtatgagg 
      gtttggttg cctctctat ccgaaagat ggcgataag 
      acgcatact tttatgtcg aagaggatt atgctaccg 
      ttccatctt aaaatatat ccgttagca attctcttt 
      gaagacgca ataattttg ggagccgag aatgacaca 
      gtcctgtat acgtccgat tcaaattcg ccgttttct 
      ctaccgttt agtgtatta caacgtact agtcaggtg 
      taccttcat caaatctta cgtcaatta atccgaaga 
      aacctcggt tatcacgcg tgggaaata gctcgaagc 
      tgcatgagt ttgccttat tttccccat tcgttagag 
      ctgttgtta cacgaggtg ttggaggag gaggccacc 
      agtaaggaa ccaattccc gatgctcaa ttaccaagc 
      gtgatagaa tttattatg gaatttccc gtctattta 
      cagacggta gtgcaatgt gcccgaaag actgaaatc 
      gctctatgg ccgtatctt ttttcggcc gccggcaaa 
      ccgaaagat ctttttcaa gaatgcatg gccaagaga 
      caactcgat acggccgct tcctacctc attattttg 
      caaaatttg gaaacttcc tctgttagt agacagtac 
      gccacgttg ttgctgaat acggccctg gatcagtcc 
      aagtgggag ctcgccaag gatctagtt cgcttcctg 
      agagccatc gacccaaac gacgttgaa tctcctcgg 
      acgtccttc atcctgccc cccaagctg ggaatctcc 
      caacaaaca ccgcccgtg agccccaac gctgaagac 
      ctctcccta attctgggc aatgtgcaa ggcccccgt 
      gtccgcagc tacagcacg acaatatcc cccaaacta 
      gtcgaaaac aggcccaat aacccgacc aaccatccg 
      attgaatca ccacaaaca gcccggaaa aaatccgta 
      ccaaacaca gttggtcgc aactccccg aataacaac 
      acagccgag gagtttttc atcgatgtt attttacaa 
      aaacacgct cgcaggtta ctttcaacc cgccgttta 
      accgatctt ggctacttt gccgcgcat ttggatttc 
      catctggtg gcttggttg ggcaaagag cgcgatcgt 
      gctgcgaga attgacgat ttcgtccag gccttgaag 
      cacctccat gaggagttc caatggcct tatccaacc 
      ccaagtgcg ttctctcca ccaaacact ggatgcgac 
      cttgaagac cgtctcaaa ttactaaaa atcgacgta 
      acgcatcca gcgtccaga gtgggggat agcggttat 
      atgagtttt caaggggtg ccttgcacg gggctaatc 
      accccgatt aacaccctg gactcggag acggagctc 
      gatgatttg tcgcaaagc gagacgaat aaaatcgac 
      gataagggt gatttagcc acgagttcg actttttac 
      gataagagg attgatttg gctcaggtt ggtggaagg 
      gttgatgat gcggcaatg agttcggtt agttcggtc 
      tggggtgag gataggatt gagtcgagt aatgataat 
      tgggaggtg ccctcaaca caaattttg gaacagttg 
      tccaatacg gataataaa gggtcacag aggtctgaa 
      gttcagttg aggtatttg ctgcaattg tttatggaa 
      gcgagttgt ttggaatgg tcgttgatt atttcggtg 
      cttttgagg gatgcgatg gctgttttg aggacagtg 
      aatgcggcc aaaagtcca gatcagtcg attgaggcg 
      gtgtcgagg ctgaggcaa gggttgctg ctgctgcag 
      tattggaca aacactgaa tgtttgggc tacaaagcc 
      ttcatgttg gcaatacaa aatcaaata tccgtcctt 
      tcgaaaatc ctcgagaca aaaatccaa catcagaag 
      atagtggag taccagcag gcagtgtct gcgtcactc 
      aataaactc tcaacgcca attccggcc cgatctcgt 
      aaaaccagc tcgaatcac gataattct agtcaaagt 
      ggttcggtc aaagaaaag aaaaacatc aacataacc 
      accagtcac agcacaacc gacaaactc aacgaaatg 
      aacccgata aattccagt agctgcgcc aaaacaata 
      aatcaggag aataataat caagtggaa gatgcaatc 
      attccggaa agcaccggc tgcgttata tcatag
    mRNA sequence
    • >TC009376 RA
      tcagttgac gtttgtgcg aagtgtcaa acgaagcca 
      caaaaactt tcccaaaaa agaatacaa taataatga 
      agtgttcaa tttgttaac ggcaacaat cagcttaaa 
      atgtgacta gttatgata gtgtggtgt tgaagctaa 
      atccaaaat gtacttccc gatcggttg gccaaaagt 
      catcaaaat accagattt aggacatgc aagtgtaag 
      gcaaataac ttgcaacag ggacaggat actgtttgc 
      tatactaac ggacgattc gatcgcgat ttggttttg 
      taaaccatg cgttccaat tgtcttcta tcgaagaac 
      cccacagtc cttggaaaa attcggaac gaacatttt 
      aaccgaatg gaagccgga ctccagcat ggtggcagt 
      cgcgacgtc agaaggcca cttgttatt gtttaaact 
      aggagtgat agctgataa taaaggttt atacgtcca 
      gactgatag cccccatgc gaatttacg tagggacag 
      cgccgaatt gttcattaa agaaataat tccgcctct 
      gcatctcac tctgcacca agaaattat ggtgtggga 
      cggtaaaat aacaggaat cgtctgtat aacaatgtc 
      agagtttat gatctcaac gagtgaagg acatgtctt 
      gaggtattg gtgggatgg acaacaaca tagagacta 
      taatttgga tttgaggag aataccttt ctgtatcaa 
      tcaacaagt gtccaaagc aatcccaat tgttgaaga 
      aaatacgtt tatagttga tatagaata ttcgccact 
      agttggcgg tttttcaat tgttttaaa tgacggaag 
      agcggcgtt tttaacagc ctcctctct caaatttga 
      tcccaatca agtccaagg aatatgggc ccagaacat 
      cgaagacgc aacttgcac cgttatgaa ccacaaata 
      ccgattaat aacttttgg tcgagccaa ttccgaatg 
      cattgtata ttacgttga cgaaagcac aggtggttt 
      ggaagtgtc tcacaattg tgttttgag tagtaaaga 
      ttatcctgg tgatcctgg agctgtagc tcaagtatt 
      gtggactcc agatggttg tgcattggt tgctgcatg 
      ggaaaaagg gggtattgc aatgtggtc aacttttgg 
      atcactttt aatgtgttc tttgggttg ggattatgg 
      gttaaatat cgatctcca gacaaataa tcccttaca 
      aattaaatc aatgcagtt cgcgacgga aggatatca 
      gttatggat ggtacataa ggaggccaa agatgggga 
      agttaatag taatgggac atgtacgaa tttgttgca 
      gttggattt tatgaaaag tgccctaac tatcaatcc 
      atgtatgag ccaccagag ccacttgta ccttcaagg 
      cgaggacaa actctacgt aaactctgc cgacacttt 
      gatcaaaat gttttcgga gcgctctac caaagacga 
      gggcgtttt caacgaatc gctctcaat gccctccac 
      tctggccga aggcagaca atggcttgt gattcccgt 
      tccttccac atactcggc aacgaattg gccgattcg 
      atattcggc gatagattc agacggtca aaacatggc 
      aatcgccgg tcgcactgg ccttgccca ctattccat 
      gcaaacacg ccgatggaa actattcgg taacgaaac 
      acaagagaa ggattttat cgtagttgg gggtttgtt 
      atggtggcg ggattacct ggtaatggg ctgctacag 
      cattttgga aaattcgga cgagttacg gttttatcc 
      aagagacgc aaagttgga taataaatt tgcgaaaat 
      tgtgactgt gttatcgcc gattctcct catgaatat 
      cctacagga tcagttgat tacgttcgg atcggatgc 
      acaagtcac gatatgggc tcttaaaca taacccgac 
      tgtcggaaa cgttgaagt ttttaaagt ccaagtcgt 
      ggatatatc agcgttggc tgtgcatcc tgcttgtat 
      cgtttcggt gactttgtc gtctttgcg gaccgaaac 
      gggccgagg ccaaccgaa taattccga aagtattgt 
      tcttaacgt aagtggtag acttttgat ggtacaaag 
      agaagtgag gaatgggga acgttatac gtgttctat 
      gccgactgt tcttgccag ttgtgtgga aaatgtctg 
      ggtgcccag tcggagaaa ggccgagaa ggctcattt 
      aaccgaagc tttatggtt gttttgtgg ggcgcatgg 
      tatgagggt ttggttgcc tctctatcc gaaagatgg 
      cgataagac gcatacttt tatgtcgaa gaggattat 
      gctaccgtt ccatcttaa aatatatcc gttagcaat 
      tctctttga agacgcaat aattttggg agccgagaa 
      tgacacagt cctgtatac gtccgattc aaattcgcc 
      gttttctct accgtttag tgtattaca acgtactag 
      tcaggtgta ccttcatca aatcttacg tcaattaat 
      ccgaagaaa cctcggtta tcacgcgtg ggaaatagc 
      tcgaagctg catgagttt gccttattt tccccattc 
      gttagagct gttgttaca cgaggtgtt ggaggagga 
      ggccaccag taaggaacc aattcccga tgctcaatt 
      accaagcgt gatagaatt tattatgga atttcccgt 
      ctatttaca gacggtagt gcaatgtgc ccgaaagac 
      tgaaatcgc tctatggcc gtatctttt ttcggccgc 
      cggcaaacc gaaagatct ttttcaaga atgcatggc 
      caagagaca actcgatac ggccgcttc ctacctcat 
      tattttgca aaatttgga aacttcctc tgttagtag 
      acagtacgc cacgttgtt gctgaatac ggccctgga 
      tcagtccaa gtgggagct cgccaagga tctagttcg 
      cttcctgag agccatcga cccaaacga cgttgaatc 
      tcctcggac gtccttcat cctgccccc caagctggg 
      aatctccca acaaacacc gcccgtgag ccccaacgc 
      tgaagacct ctccctaat tctgggcaa tgtgcaagg 
      cccccgtgt ccgcagcta cagcacgac aatatcccc 
      caaactagt cgaaaacag gcccaataa cccgaccaa 
      ccatccgat tgaatcacc acaaacagc ccggaaaaa 
      atccgtacc aaacacagt tggtcgcaa ctccccgaa 
      taacaacac agccgagga gtttttcat cgatgttat 
      tttacaaaa acacgctcg caggttact ttcaacccg 
      ccgtttaac cgatcttgg ctactttgc cgcgcattt 
      ggatttcca tctggtggc ttggttggg caaagagcg 
      cgatcgtgc tgcgagaat tgacgattt cgtccaggc 
      cttgaagca cctccatga ggagttcca atggcctta 
      tccaacccc aagtgcgtt ctctccacc aaacactgg 
      atgcgacct tgaagaccg tctcaaatt actaaaaat 
      cgacgtaac gcatccagc gtccagagt gggggatag 
      cggttatat gagttttca aggggtgcc ttgcacggg 
      gctaatcac cccgattaa caccctgga ctcggagac 
      ggagctcga tgatttgtc gcaaagcga gacgaataa 
      aatcgacga taagggtga tttagccac gagttcgac 
      tttttacga taagaggat tgatttggc tcaggttgg 
      tggaagggt tgatgatgc ggcaatgag ttcggttag 
      ttcggtctg gggtgagga taggattga gtcgagtaa 
      tgataattg ggaggtgcc ctcaacaca aattttgga 
      acagttgtc caatacgga taataaagg gtcacagag 
      gtctgaagt tcagttgag gtatttgct gcaattgtt 
      tatggaagc gagttgttt ggaatggtc gttgattat 
      ttcggtgct tttgaggga tgcgatggc tgttttgag 
      gacagtgaa tgcggccaa aagtccaga tcagtcgat 
      tgaggcggt gtcgaggct gaggcaagg gttgctgct 
      gctgcagta ttggacaaa cactgaatg tttgggcta 
      caaagcctt catgttggc aatacaaaa tcaaatatc 
      cgtcctttc gaaaatcct cgagacaaa aatccaaca 
      tcagaagat agtggagta ccagcaggc agtgtctgc 
      gtcactcaa taaactctc aacgccaat tccggcccg 
      atctcgtaa aaccagctc gaatcacga taattctag 
      tcaaagtgg ttcggtcaa agaaaagaa aaacatcaa 
      cataaccac cagtcacag cacaaccga caaactcaa 
      cgaaatgaa cccgataaa ttccagtag ctgcgccaa 
      aacaataaa tcaggagaa taataatca agtggaaga 
      tgcaatcat tccggaaag caccggctg cgttatatc 
      ataggtttt ttacgaaat aaatttttg ggtgtacta 
      tttgtggac actcggaac tgcctaaaa atgcgatct 
      aatccgtct tagaagtga taggtacgt aacaaatgt 
      tgttgcact tttttgttt tacgtaaaa atgaaaccg 
      ttttcaatt gtgttgtgc ctcgcattt atgaaacgt 
      cgtgcattc cgctgaaag aaatatttt tgcaaacga 
      tattaccgt atgagtttt tcagcaata acactagtt 
      attcgtagc aattcggtc cgaacaatc gacttgggt 
      gataaggtg ttgaattat taatgttgt aggttttaa 
      aatctgttt ctaataata ttgttggtc aatttttat 
      acaataatc gtagactgt cgtcttatt aatactcca 
      ctatattgt tgtataatt tattacttt tttcaacag 
      ttttatttt gtaacatac taaaaataa ataacagtt 
      ttattc
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