| General information | |||
| TC033522
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1068396..1106326 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033522 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04622 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC033522 RA
MCESDLRIE DFSDLPKKP FVRGSDPRV ATCRGKLQN RRSKLNQEI NKELRLRAG AENLFKATT NRKLKETVA LELSFVNSN LQLLKEQLA ELNSSVELY QSDSTDAVM PMIPLGLKE TKEIDFRDP FKDFILEHY SEDANQYED AIADFMDTR QAIRTPLRD TTGISLLFR YYNQLYFVE RRFFPPDRS LGIYFEWFD SLTGVPSCQ RTVAFEKAC VLFNMGAIY TQIGAKQDR STAKGLDAA VDSFLRAAG TFRYIHENF TNAPSMDLG PEMLEMLVQ LMLAQAREC LLEKLDLQS KESRSIDIC LDLAQEAAH LADCYSQVY QLIMTESVH DYVPYSWIS LVQVKKEYY TGMAHYHVA SGVLHKEAA KMSPTTKTT LQFLHAETA STQLDIRLP KDDAERRLL GRAHLRESL VLHEECQRL HRMCRELRG KQALTAVLR NAHKTALQA YTNNENEDD FSDVLDPPR VQAATKFQL ALTPPDFAQ YRVNDLFRA LGPIAIFSA KRHWTAPRL VQLHRGRTS EGFGFSVRG DAPVIVAIV ESNSLAEFG GVKEGDFIV AISDKDVKW SSHDEVVAL IKNAGDSLS LKLVTPMDR NYLKPTKST KGSISTHSS SSGVSSGMS SPSGSMAQH KNNKRLGWI PFKRHSSSR EGKDFFENV ILR
- >TC033522 RB
MLKWVQRHN FKCPAEEDF VIIPGSDPR VATCRGKLQ NRRSKLNQE INKELRLRA GAENLFKAT TNRKLKETV ALELSFVNS NLQLLKEQL AELNSSVEL YQSDSTDAV MPMIPLGLK ETKEIDFRD PFKDFILEH YSEDANQYE DAIADFMDT RQAIRTPLR DTTGISLLF RYYNQLYFV ERRFFPPDR SLGIYFEWF DSLTGVPSC QRTVAFEKA CVLFNMGAI YTQIGAKQD RSTAKGLDA AVDSFLRAA GTFRYIHEN FTNAPSMDL GPEMLEMLV QLMLAQARE CLLEKLDLQ SKESRSIDI CLDLAQEAA HLADCYSQV YQLIMTESV HDYVPYSWI SLVQVKKEY YTGMAHYHV ASGVLHKEA AKMSPTTKT TLQFLHAET ASTQLDIRL PKDDAERRL LGRAHLRES LVLHEECQR LHRMCRELR GKQALTAVL RNAHKTALQ AYTNNENED DFSDVLDPP RVQAATKFQ LALTPPDFA QYRVNDLFR ALGPIAIFS AKRHWTAPR LVQLHRGRT SEGFGFSVR GDAPVIVAI VESNSLAEF GGVKEGDFI VAISDKDVK WSSHDEVVA LIKNAGDSL SLKLVTPMD RNYLKPTKS TKGSISTHS SSSGVSSGM SSPSGSMAQ HKNNKRLGW IPFKRHSSS REGKDFFEN VILR
- >TC033522 RA
atgtgcgag agtgatttg cgaatagag gacttcagc gacttgccg aagaagccc ttcgtacga ggatctgat ccccgggtg gcaacatgt cgaggaaaa ctccaaaat cggcgctcg aagctcaac caggagatc aacaaggag ttgaggctg agagctgga gccgagaat ttattcaaa gctacgacg aatcgcaag ttgaaggaa actgtggct ttggagctc agtttcgtc aactccaac ctccagctg ctgaaggag cagctggcg gagttgaac tcgtcggtg gagctgtac caaagcgac agcaccgac gctgtcatg cccatgatc ccccttggc ctgaaagag acaaaagag atcgacttc cgggacccc ttcaaggac ttcatcctg gagcactac agcgaagac gccaaccaa tacgaagat gccatagcc gacttcatg gacacgcgc caggccata aggacaccc ttgcgggac accaccggc atatccctg ctgttccgc tactacaac cagttgtat ttcgttgag agacgtttt ttcccgcct gacagatcc ctcgggatc tacttcgag tggttcgat tcgctaacg ggagtgcca tcgtgtcaa aggactgta gctttcgaa aaagcgtgc gttttgttc aatatgggc gcgatttac acgcaaatt ggtgctaaa caggaccgt tccacggcg aagggattg gatgcagcc gttgatagt tttctaagg gctgcaggg acgtttcga tacattcat gaaaatttc acaaatgcg ccgtcgatg gacttgggc cctgaaatg ctggaaatg ctcgtgcaa ctcatgctg gcccaagcc cgcgaatgc ctcctcgaa aagctcgac ctgcagtca aaagaaagc cgctcgatc gacatctgc ctcgacctt gcccaagaa gctgcccat ctggcagac tgctacagc caggtctat caactcata atgaccgaa tccgtccac gactacgtc ccctacagc tggatctct ttagtccaa gtaaaaaag gaatattac accggcatg gcccattac catgttgct tccggcgta ctgcacaaa gaagcggca aaaatgtca cccaccacc aaaaccacc ctccagttt ttacacgcg gaaacggcc tccacacag ctcgacatc aggttaccg aaagacgac gccgaacga agactttta ggtcgcgcg cacttgaga gagtcccta gtcctgcac gaggagtgc cagaggttg caccggatg tgtcgcgag ctgagaggg aagcaggcc cttacagcg gtcctaaga aatgcccac aaaacggcc cttcaggcc tacacgaac aacgagaat gaggacgac ttcagcgat gttctggac ccgccccgg gtgcaagct gcgaccaaa ttccaactg gctttgacg ccccctgac ttcgcccag taccgcgtc aacgacctt ttccgggct ttgggcccc attgcgatt ttctcggcg aaaaggcac tggacggcg ccccgtctc gtgcaacta caccgtggc cgaacctct gaaggtttc ggcttttca gtccgagga gacgcccca gtcattgtt gcaatagtt gaatcaaat tcacttgca gagttcggc ggcgtcaaa gagggtgac ttcatcgtg gcaattagc gataaagat gttaaatgg tcctcgcac gatgaagtc gtggcgctt atcaaaaac gccggcgat tccttaagt ctcaagctg gtcactccg atggacagg aattatctt aaaccgaca aagtcgaca aaagggtcc atttcgacg catagtagt agttcgggg gtttctagc gggatgtct agtccgtca gggtccatg gcacagcac aaaaataat aaaaggttg gggtggatt ccgtttaaa cggcattcc tcgtcaagg gagggcaaa gattttttc gagaatgtt attttgaga tga
- >TC033522 RB
atgctgaag tgggtgcag agacacaac ttcaagtgc cctgctgag gaggatttt gtcatcatt ccgggatct gatccccgg gtggcaaca tgtcgagga aaactccaa aatcggcgc tcgaagctc aaccaggag atcaacaag gagttgagg ctgagagct ggagccgag aatttattc aaagctacg acgaatcgc aagttgaag gaaactgtg gctttggag ctcagtttc gtcaactcc aacctccag ctgctgaag gagcagctg gcggagttg aactcgtcg gtggagctg taccaaagc gacagcacc gacgctgtc atgcccatg atccccctt ggcctgaaa gagacaaaa gagatcgac ttccgggac cccttcaag gacttcatc ctggagcac tacagcgaa gacgccaac caatacgaa gatgccata gccgacttc atggacacg cgccaggcc ataaggaca cccttgcgg gacaccacc ggcatatcc ctgctgttc cgctactac aaccagttg tatttcgtt gagagacgt tttttcccg cctgacaga tccctcggg atctacttc gagtggttc gattcgcta acgggagtg ccatcgtgt caaaggact gtagctttc gaaaaagcg tgcgttttg ttcaatatg ggcgcgatt tacacgcaa attggtgct aaacaggac cgttccacg gcgaaggga ttggatgca gccgttgat agttttcta agggctgca gggacgttt cgatacatt catgaaaat ttcacaaat gcgccgtcg atggacttg ggccctgaa atgctggaa atgctcgtg caactcatg ctggcccaa gcccgcgaa tgcctcctc gaaaagctc gacctgcag tcaaaagaa agccgctcg atcgacatc tgcctcgac cttgcccaa gaagctgcc catctggca gactgctac agccaggtc tatcaactc ataatgacc gaatccgtc cacgactac gtcccctac agctggatc tctttagtc caagtaaaa aaggaatat tacaccggc atggcccat taccatgtt gcttccggc gtactgcac aaagaagcg gcaaaaatg tcacccacc accaaaacc accctccag tttttacac gcggaaacg gcctccaca cagctcgac atcaggtta ccgaaagac gacgccgaa cgaagactt ttaggtcgc gcgcacttg agagagtcc ctagtcctg cacgaggag tgccagagg ttgcaccgg atgtgtcgc gagctgaga gggaagcag gcccttaca gcggtccta agaaatgcc cacaaaacg gcccttcag gcctacacg aacaacgag aatgaggac gacttcagc gatgttctg gacccgccc cgggtgcaa gctgcgacc aaattccaa ctggctttg acgccccct gacttcgcc cagtaccgc gtcaacgac cttttccgg gctttgggc cccattgcg attttctcg gcgaaaagg cactggacg gcgccccgt ctcgtgcaa ctacaccgt ggccgaacc tctgaaggt ttcggcttt tcagtccga ggagacgcc ccagtcatt gttgcaata gttgaatca aattcactt gcagagttc ggcggcgtc aaagagggt gacttcatc gtggcaatt agcgataaa gatgttaaa tggtcctcg cacgatgaa gtcgtggcg cttatcaaa aacgccggc gattcctta agtctcaag ctggtcact ccgatggac aggaattat cttaaaccg acaaagtcg acaaaaggg tccatttcg acgcatagt agtagttcg ggggtttct agcgggatg tctagtccg tcagggtcc atggcacag cacaaaaat aataaaagg ttggggtgg attccgttt aaacggcat tcctcgtca agggagggc aaagatttt ttcgagaat gttattttg agatga
- >TC033522 RA
aagcattgt cgttcacgt tgttcacct ctacggaca cgcttctga tatttccgc gccgctttt cgaactagg tcgcagatt catcttctt cacagtgac agtgcagga aaaaagtga gaagggaaa caatgtgaa gatgtgcga gagtgattt gcgaataga ggacttcag cgacttgcc gaagaagcc cttcgtacg aggatctga tccccgggt ggcaacatg tcgaggaaa actccaaaa tcggcgctc gaagctcaa ccaggagat caacaagga gttgaggct gagagctgg agccgagaa tttattcaa agctacgac gaatcgcaa gttgaagga aactgtggc tttggagct cagtttcgt caactccaa cctccagct gctgaagga gcagctggc ggagttgaa ctcgtcggt ggagctgta ccaaagcga cagcaccga cgctgtcat gcccatgat cccccttgg cctgaaaga gacaaaaga gatcgactt ccgggaccc cttcaagga cttcatcct ggagcacta cagcgaaga cgccaacca atacgaaga tgccatagc cgacttcat ggacacgcg ccaggccat aaggacacc cttgcggga caccaccgg catatccct gctgttccg ctactacaa ccagttgta tttcgttga gagacgttt tttcccgcc tgacagatc cctcgggat ctacttcga gtggttcga ttcgctaac gggagtgcc atcgtgtca aaggactgt agctttcga aaaagcgtg cgttttgtt caatatggg cgcgattta cacgcaaat tggtgctaa acaggaccg ttccacggc gaagggatt ggatgcagc cgttgatag ttttctaag ggctgcagg gacgtttcg atacattca tgaaaattt cacaaatgc gccgtcgat ggacttggg ccctgaaat gctggaaat gctcgtgca actcatgct ggcccaagc ccgcgaatg cctcctcga aaagctcga cctgcagtc aaaagaaag ccgctcgat cgacatctg cctcgacct tgcccaaga agctgccca tctggcaga ctgctacag ccaggtcta tcaactcat aatgaccga atccgtcca cgactacgt cccctacag ctggatctc tttagtcca agtaaaaaa ggaatatta caccggcat ggcccatta ccatgttgc ttccggcgt actgcacaa agaagcggc aaaaatgtc acccaccac caaaaccac cctccagtt tttacacgc ggaaacggc ctccacaca gctcgacat caggttacc gaaagacga cgccgaacg aagactttt aggtcgcgc gcacttgag agagtccct agtcctgca cgaggagtg ccagaggtt gcaccggat gtgtcgcga gctgagagg gaagcaggc ccttacagc ggtcctaag aaatgccca caaaacggc ccttcaggc ctacacgaa caacgagaa tgaggacga cttcagcga tgttctgga cccgccccg ggtgcaagc tgcgaccaa attccaact ggctttgac gccccctga cttcgccca gtaccgcgt caacgacct tttccgggc tttgggccc cattgcgat tttctcggc gaaaaggca ctggacggc gccccgtct cgtgcaact acaccgtgg ccgaacctc tgaaggttt cggcttttc agtccgagg agacgcccc agtcattgt tgcaatagt tgaatcaaa ttcacttgc agagttcgg cggcgtcaa agagggtga cttcatcgt ggcaattag cgataaaga tgttaaatg gtcctcgca cgatgaagt cgtggcgct tatcaaaaa cgccggcga ttccttaag tctcaagct ggtcactcc gatggacag gaattatct taaaccgac aaagtcgac aaaagggtc catttcgac gcatagtag tagttcggg ggtttctag cgggatgtc tagtccgtc agggtccat ggcacagca caaaaataa taaaaggtt ggggtggat tccgtttaa acggcattc ctcgtcaag ggagggcaa agatttttt cgagaatgt tattttgag atgaattat tgtgtagtt atatcgact ttatgtcac tgtactgat acgagattt atttatttt tttatcgta actacggat aagtaaaat gaatgattg agatgtcgt aatgtgtac attaacatt cttaaacat tattattta tagtcggca ctgtacaac acaattttt attatttat gtattagag atttatatt gttatttgt ttgtacatt gtatttatg aatgatgtc tacgataga acccaaata aaaactata ttcaaac
- >TC033522 RB
aacaggttt tattgtatt tacaataat gcaacgaca cgcttaatg actgtatta gaatccgaa cggtggcta aggcactta tctgccatt tgtcggaga gataagacg cacaagtgg cgcttccgc caaaaataa tgtgactgt gtgcaaaat gctgaagtg ggtgcagag acacaactt caagtgccc tgctgagga ggattttgt catcattcc gaatccgaa cggtggcta aggcactta tctgccatt tgtcggaga gataagacg cacaagtgg cgcttccgc caaaaataa tgtgactgt gtgcaaaat gctgaagtg ggtgcagag acacaactt caagtgccc tgctgagga ggattttgt catcattcc gggatctga tccccgggt ggcaacatg tcgaggaaa actccaaaa tcggcgctc gaagctcaa ccaggagat caacaagga gttgaggct gagagctgg agccgagaa tttattcaa agctacgac gaatcgcaa gttgaagga aactgtggc tttggagct cagtttcgt caactccaa cctccagct gctgaagga gcagctggc ggagttgaa ctcgtcggt ggagctgta ccaaagcga cagcaccga cgctgtcat gcccatgat cccccttgg cctgaaaga gacaaaaga gatcgactt ccgggaccc cttcaagga cttcatcct ggagcacta cagcgaaga cgccaacca atacgaaga tgccatagc cgacttcat ggacacgcg ccaggccat aaggacacc cttgcggga caccaccgg catatccct gctgttccg ctactacaa ccagttgta tttcgttga gagacgttt tttcccgcc tgacagatc cctcgggat ctacttcga gtggttcga ttcgctaac gggagtgcc atcgtgtca aaggactgt agctttcga aaaagcgtg cgttttgtt caatatggg cgcgattta cacgcaaat tggtgctaa acaggaccg ttccacggc gaagggatt ggatgcagc cgttgatag ttttctaag ggctgcagg gacgtttcg atacattca tgaaaattt cacaaatgc gccgtcgat ggacttggg ccctgaaat gctggaaat gctcgtgca actcatgct ggcccaagc ccgcgaatg cctcctcga aaagctcga cctgcagtc aaaagaaag ccgctcgat cgacatctg cctcgacct tgcccaaga agctgccca tctggcaga ctgctacag ccaggtcta tcaactcat aatgaccga atccgtcca cgactacgt cccctacag ctggatctc tttagtcca agtaaaaaa ggaatatta caccggcat ggcccatta ccatgttgc ttccggcgt actgcacaa agaagcggc aaaaatgtc acccaccac caaaaccac cctccagtt tttacacgc ggaaacggc ctccacaca gctcgacat caggttacc gaaagacga cgccgaacg aagactttt aggtcgcgc gcacttgag agagtccct agtcctgca cgaggagtg ccagaggtt gcaccggat gtgtcgcga gctgagagg gaagcaggc ccttacagc ggtcctaag aaatgccca caaaacggc ccttcaggc ctacacgaa caacgagaa tgaggacga cttcagcga tgttctgga cccgccccg ggtgcaagc tgcgaccaa attccaact ggctttgac gccccctga cttcgccca gtaccgcgt caacgacct tttccgggc tttgggccc cattgcgat tttctcggc gaaaaggca ctggacggc gccccgtct cgtgcaact acaccgtgg ccgaacctc tgaaggttt cggcttttc agtccgagg agacgcccc agtcattgt tgcaatagt tgaatcaaa ttcacttgc agagttcgg cggcgtcaa agagggtga cttcatcgt ggcaattag cgataaaga tgttaaatg gtcctcgca cgatgaagt cgtggcgct tatcaaaaa cgccggcga ttccttaag tctcaagct ggtcactcc gatggacag gaattatct taaaccgac aaagtcgac aaaagggtc catttcgac gcatagtag tagttcggg ggtttctag cgggatgtc tagtccgtc agggtccat ggcacagca caaaaataa taaaaggtt ggggtggat tccgtttaa acggcattc ctcgtcaag ggagggcaa agatttttt cgagaatgt tattttgag atgaattat tgtgtagtt atatcgact ttatgtcac tgtactgat acgagattt atttatttt tttatcgta actacggat aagtaaaat gaatgattg agatgtcgt aatgtgtac attaacatt cttaaacat tattattta tagtcggca ctgtacaac acaattttt attatttat gtattagag atttatatt gttatttgt ttgtacatt gtatttatg aatgatgtc tacgataga acccaaata aaaactata ttcaaac
