Status
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Gene information
General information
TC033522
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009368,TC009367
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1068396..1106326
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033522
    iBeetle screen iB_04622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033522 RA
      MCESDLRIE DFSDLPKKP FVRGSDPRV ATCRGKLQN 
      RRSKLNQEI NKELRLRAG AENLFKATT NRKLKETVA 
      LELSFVNSN LQLLKEQLA ELNSSVELY QSDSTDAVM 
      PMIPLGLKE TKEIDFRDP FKDFILEHY SEDANQYED 
      AIADFMDTR QAIRTPLRD TTGISLLFR YYNQLYFVE 
      RRFFPPDRS LGIYFEWFD SLTGVPSCQ RTVAFEKAC 
      VLFNMGAIY TQIGAKQDR STAKGLDAA VDSFLRAAG 
      TFRYIHENF TNAPSMDLG PEMLEMLVQ LMLAQAREC 
      LLEKLDLQS KESRSIDIC LDLAQEAAH LADCYSQVY 
      QLIMTESVH DYVPYSWIS LVQVKKEYY TGMAHYHVA 
      SGVLHKEAA KMSPTTKTT LQFLHAETA STQLDIRLP 
      KDDAERRLL GRAHLRESL VLHEECQRL HRMCRELRG 
      KQALTAVLR NAHKTALQA YTNNENEDD FSDVLDPPR 
      VQAATKFQL ALTPPDFAQ YRVNDLFRA LGPIAIFSA 
      KRHWTAPRL VQLHRGRTS EGFGFSVRG DAPVIVAIV 
      ESNSLAEFG GVKEGDFIV AISDKDVKW SSHDEVVAL 
      IKNAGDSLS LKLVTPMDR NYLKPTKST KGSISTHSS 
      SSGVSSGMS SPSGSMAQH KNNKRLGWI PFKRHSSSR 
      EGKDFFENV ILR
    • >TC033522 RB
      MLKWVQRHN FKCPAEEDF VIIPGSDPR VATCRGKLQ 
      NRRSKLNQE INKELRLRA GAENLFKAT TNRKLKETV 
      ALELSFVNS NLQLLKEQL AELNSSVEL YQSDSTDAV 
      MPMIPLGLK ETKEIDFRD PFKDFILEH YSEDANQYE 
      DAIADFMDT RQAIRTPLR DTTGISLLF RYYNQLYFV 
      ERRFFPPDR SLGIYFEWF DSLTGVPSC QRTVAFEKA 
      CVLFNMGAI YTQIGAKQD RSTAKGLDA AVDSFLRAA 
      GTFRYIHEN FTNAPSMDL GPEMLEMLV QLMLAQARE 
      CLLEKLDLQ SKESRSIDI CLDLAQEAA HLADCYSQV 
      YQLIMTESV HDYVPYSWI SLVQVKKEY YTGMAHYHV 
      ASGVLHKEA AKMSPTTKT TLQFLHAET ASTQLDIRL 
      PKDDAERRL LGRAHLRES LVLHEECQR LHRMCRELR 
      GKQALTAVL RNAHKTALQ AYTNNENED DFSDVLDPP 
      RVQAATKFQ LALTPPDFA QYRVNDLFR ALGPIAIFS 
      AKRHWTAPR LVQLHRGRT SEGFGFSVR GDAPVIVAI 
      VESNSLAEF GGVKEGDFI VAISDKDVK WSSHDEVVA 
      LIKNAGDSL SLKLVTPMD RNYLKPTKS TKGSISTHS 
      SSSGVSSGM SSPSGSMAQ HKNNKRLGW IPFKRHSSS 
      REGKDFFEN VILR
    CDS sequence
    • >TC033522 RA
      atgtgcgag agtgatttg cgaatagag gacttcagc 
      gacttgccg aagaagccc ttcgtacga ggatctgat 
      ccccgggtg gcaacatgt cgaggaaaa ctccaaaat 
      cggcgctcg aagctcaac caggagatc aacaaggag 
      ttgaggctg agagctgga gccgagaat ttattcaaa 
      gctacgacg aatcgcaag ttgaaggaa actgtggct 
      ttggagctc agtttcgtc aactccaac ctccagctg 
      ctgaaggag cagctggcg gagttgaac tcgtcggtg 
      gagctgtac caaagcgac agcaccgac gctgtcatg 
      cccatgatc ccccttggc ctgaaagag acaaaagag 
      atcgacttc cgggacccc ttcaaggac ttcatcctg 
      gagcactac agcgaagac gccaaccaa tacgaagat 
      gccatagcc gacttcatg gacacgcgc caggccata 
      aggacaccc ttgcgggac accaccggc atatccctg 
      ctgttccgc tactacaac cagttgtat ttcgttgag 
      agacgtttt ttcccgcct gacagatcc ctcgggatc 
      tacttcgag tggttcgat tcgctaacg ggagtgcca 
      tcgtgtcaa aggactgta gctttcgaa aaagcgtgc 
      gttttgttc aatatgggc gcgatttac acgcaaatt 
      ggtgctaaa caggaccgt tccacggcg aagggattg 
      gatgcagcc gttgatagt tttctaagg gctgcaggg 
      acgtttcga tacattcat gaaaatttc acaaatgcg 
      ccgtcgatg gacttgggc cctgaaatg ctggaaatg 
      ctcgtgcaa ctcatgctg gcccaagcc cgcgaatgc 
      ctcctcgaa aagctcgac ctgcagtca aaagaaagc 
      cgctcgatc gacatctgc ctcgacctt gcccaagaa 
      gctgcccat ctggcagac tgctacagc caggtctat 
      caactcata atgaccgaa tccgtccac gactacgtc 
      ccctacagc tggatctct ttagtccaa gtaaaaaag 
      gaatattac accggcatg gcccattac catgttgct 
      tccggcgta ctgcacaaa gaagcggca aaaatgtca 
      cccaccacc aaaaccacc ctccagttt ttacacgcg 
      gaaacggcc tccacacag ctcgacatc aggttaccg 
      aaagacgac gccgaacga agactttta ggtcgcgcg 
      cacttgaga gagtcccta gtcctgcac gaggagtgc 
      cagaggttg caccggatg tgtcgcgag ctgagaggg 
      aagcaggcc cttacagcg gtcctaaga aatgcccac 
      aaaacggcc cttcaggcc tacacgaac aacgagaat 
      gaggacgac ttcagcgat gttctggac ccgccccgg 
      gtgcaagct gcgaccaaa ttccaactg gctttgacg 
      ccccctgac ttcgcccag taccgcgtc aacgacctt 
      ttccgggct ttgggcccc attgcgatt ttctcggcg 
      aaaaggcac tggacggcg ccccgtctc gtgcaacta 
      caccgtggc cgaacctct gaaggtttc ggcttttca 
      gtccgagga gacgcccca gtcattgtt gcaatagtt 
      gaatcaaat tcacttgca gagttcggc ggcgtcaaa 
      gagggtgac ttcatcgtg gcaattagc gataaagat 
      gttaaatgg tcctcgcac gatgaagtc gtggcgctt 
      atcaaaaac gccggcgat tccttaagt ctcaagctg 
      gtcactccg atggacagg aattatctt aaaccgaca 
      aagtcgaca aaagggtcc atttcgacg catagtagt 
      agttcgggg gtttctagc gggatgtct agtccgtca 
      gggtccatg gcacagcac aaaaataat aaaaggttg 
      gggtggatt ccgtttaaa cggcattcc tcgtcaagg 
      gagggcaaa gattttttc gagaatgtt attttgaga 
      tga
    • >TC033522 RB
      atgctgaag tgggtgcag agacacaac ttcaagtgc 
      cctgctgag gaggatttt gtcatcatt ccgggatct 
      gatccccgg gtggcaaca tgtcgagga aaactccaa 
      aatcggcgc tcgaagctc aaccaggag atcaacaag 
      gagttgagg ctgagagct ggagccgag aatttattc 
      aaagctacg acgaatcgc aagttgaag gaaactgtg 
      gctttggag ctcagtttc gtcaactcc aacctccag 
      ctgctgaag gagcagctg gcggagttg aactcgtcg 
      gtggagctg taccaaagc gacagcacc gacgctgtc 
      atgcccatg atccccctt ggcctgaaa gagacaaaa 
      gagatcgac ttccgggac cccttcaag gacttcatc 
      ctggagcac tacagcgaa gacgccaac caatacgaa 
      gatgccata gccgacttc atggacacg cgccaggcc 
      ataaggaca cccttgcgg gacaccacc ggcatatcc 
      ctgctgttc cgctactac aaccagttg tatttcgtt 
      gagagacgt tttttcccg cctgacaga tccctcggg 
      atctacttc gagtggttc gattcgcta acgggagtg 
      ccatcgtgt caaaggact gtagctttc gaaaaagcg 
      tgcgttttg ttcaatatg ggcgcgatt tacacgcaa 
      attggtgct aaacaggac cgttccacg gcgaaggga 
      ttggatgca gccgttgat agttttcta agggctgca 
      gggacgttt cgatacatt catgaaaat ttcacaaat 
      gcgccgtcg atggacttg ggccctgaa atgctggaa 
      atgctcgtg caactcatg ctggcccaa gcccgcgaa 
      tgcctcctc gaaaagctc gacctgcag tcaaaagaa 
      agccgctcg atcgacatc tgcctcgac cttgcccaa 
      gaagctgcc catctggca gactgctac agccaggtc 
      tatcaactc ataatgacc gaatccgtc cacgactac 
      gtcccctac agctggatc tctttagtc caagtaaaa 
      aaggaatat tacaccggc atggcccat taccatgtt 
      gcttccggc gtactgcac aaagaagcg gcaaaaatg 
      tcacccacc accaaaacc accctccag tttttacac 
      gcggaaacg gcctccaca cagctcgac atcaggtta 
      ccgaaagac gacgccgaa cgaagactt ttaggtcgc 
      gcgcacttg agagagtcc ctagtcctg cacgaggag 
      tgccagagg ttgcaccgg atgtgtcgc gagctgaga 
      gggaagcag gcccttaca gcggtccta agaaatgcc 
      cacaaaacg gcccttcag gcctacacg aacaacgag 
      aatgaggac gacttcagc gatgttctg gacccgccc 
      cgggtgcaa gctgcgacc aaattccaa ctggctttg 
      acgccccct gacttcgcc cagtaccgc gtcaacgac 
      cttttccgg gctttgggc cccattgcg attttctcg 
      gcgaaaagg cactggacg gcgccccgt ctcgtgcaa 
      ctacaccgt ggccgaacc tctgaaggt ttcggcttt 
      tcagtccga ggagacgcc ccagtcatt gttgcaata 
      gttgaatca aattcactt gcagagttc ggcggcgtc 
      aaagagggt gacttcatc gtggcaatt agcgataaa 
      gatgttaaa tggtcctcg cacgatgaa gtcgtggcg 
      cttatcaaa aacgccggc gattcctta agtctcaag 
      ctggtcact ccgatggac aggaattat cttaaaccg 
      acaaagtcg acaaaaggg tccatttcg acgcatagt 
      agtagttcg ggggtttct agcgggatg tctagtccg 
      tcagggtcc atggcacag cacaaaaat aataaaagg 
      ttggggtgg attccgttt aaacggcat tcctcgtca 
      agggagggc aaagatttt ttcgagaat gttattttg 
      agatga
    mRNA sequence
    • >TC033522 RA
      aagcattgt cgttcacgt tgttcacct ctacggaca 
      cgcttctga tatttccgc gccgctttt cgaactagg 
      tcgcagatt catcttctt cacagtgac agtgcagga 
      aaaaagtga gaagggaaa caatgtgaa gatgtgcga 
      gagtgattt gcgaataga ggacttcag cgacttgcc 
      gaagaagcc cttcgtacg aggatctga tccccgggt 
      ggcaacatg tcgaggaaa actccaaaa tcggcgctc 
      gaagctcaa ccaggagat caacaagga gttgaggct 
      gagagctgg agccgagaa tttattcaa agctacgac 
      gaatcgcaa gttgaagga aactgtggc tttggagct 
      cagtttcgt caactccaa cctccagct gctgaagga 
      gcagctggc ggagttgaa ctcgtcggt ggagctgta 
      ccaaagcga cagcaccga cgctgtcat gcccatgat 
      cccccttgg cctgaaaga gacaaaaga gatcgactt 
      ccgggaccc cttcaagga cttcatcct ggagcacta 
      cagcgaaga cgccaacca atacgaaga tgccatagc 
      cgacttcat ggacacgcg ccaggccat aaggacacc 
      cttgcggga caccaccgg catatccct gctgttccg 
      ctactacaa ccagttgta tttcgttga gagacgttt 
      tttcccgcc tgacagatc cctcgggat ctacttcga 
      gtggttcga ttcgctaac gggagtgcc atcgtgtca 
      aaggactgt agctttcga aaaagcgtg cgttttgtt 
      caatatggg cgcgattta cacgcaaat tggtgctaa 
      acaggaccg ttccacggc gaagggatt ggatgcagc 
      cgttgatag ttttctaag ggctgcagg gacgtttcg 
      atacattca tgaaaattt cacaaatgc gccgtcgat 
      ggacttggg ccctgaaat gctggaaat gctcgtgca 
      actcatgct ggcccaagc ccgcgaatg cctcctcga 
      aaagctcga cctgcagtc aaaagaaag ccgctcgat 
      cgacatctg cctcgacct tgcccaaga agctgccca 
      tctggcaga ctgctacag ccaggtcta tcaactcat 
      aatgaccga atccgtcca cgactacgt cccctacag 
      ctggatctc tttagtcca agtaaaaaa ggaatatta 
      caccggcat ggcccatta ccatgttgc ttccggcgt 
      actgcacaa agaagcggc aaaaatgtc acccaccac 
      caaaaccac cctccagtt tttacacgc ggaaacggc 
      ctccacaca gctcgacat caggttacc gaaagacga 
      cgccgaacg aagactttt aggtcgcgc gcacttgag 
      agagtccct agtcctgca cgaggagtg ccagaggtt 
      gcaccggat gtgtcgcga gctgagagg gaagcaggc 
      ccttacagc ggtcctaag aaatgccca caaaacggc 
      ccttcaggc ctacacgaa caacgagaa tgaggacga 
      cttcagcga tgttctgga cccgccccg ggtgcaagc 
      tgcgaccaa attccaact ggctttgac gccccctga 
      cttcgccca gtaccgcgt caacgacct tttccgggc 
      tttgggccc cattgcgat tttctcggc gaaaaggca 
      ctggacggc gccccgtct cgtgcaact acaccgtgg 
      ccgaacctc tgaaggttt cggcttttc agtccgagg 
      agacgcccc agtcattgt tgcaatagt tgaatcaaa 
      ttcacttgc agagttcgg cggcgtcaa agagggtga 
      cttcatcgt ggcaattag cgataaaga tgttaaatg 
      gtcctcgca cgatgaagt cgtggcgct tatcaaaaa 
      cgccggcga ttccttaag tctcaagct ggtcactcc 
      gatggacag gaattatct taaaccgac aaagtcgac 
      aaaagggtc catttcgac gcatagtag tagttcggg 
      ggtttctag cgggatgtc tagtccgtc agggtccat 
      ggcacagca caaaaataa taaaaggtt ggggtggat 
      tccgtttaa acggcattc ctcgtcaag ggagggcaa 
      agatttttt cgagaatgt tattttgag atgaattat 
      tgtgtagtt atatcgact ttatgtcac tgtactgat 
      acgagattt atttatttt tttatcgta actacggat 
      aagtaaaat gaatgattg agatgtcgt aatgtgtac 
      attaacatt cttaaacat tattattta tagtcggca 
      ctgtacaac acaattttt attatttat gtattagag 
      atttatatt gttatttgt ttgtacatt gtatttatg 
      aatgatgtc tacgataga acccaaata aaaactata 
      ttcaaac
    • >TC033522 RB
      aacaggttt tattgtatt tacaataat gcaacgaca 
      cgcttaatg actgtatta gaatccgaa cggtggcta 
      aggcactta tctgccatt tgtcggaga gataagacg 
      cacaagtgg cgcttccgc caaaaataa tgtgactgt 
      gtgcaaaat gctgaagtg ggtgcagag acacaactt 
      caagtgccc tgctgagga ggattttgt catcattcc 
      gaatccgaa cggtggcta aggcactta tctgccatt 
      tgtcggaga gataagacg cacaagtgg cgcttccgc 
      caaaaataa tgtgactgt gtgcaaaat gctgaagtg 
      ggtgcagag acacaactt caagtgccc tgctgagga 
      ggattttgt catcattcc gggatctga tccccgggt 
      ggcaacatg tcgaggaaa actccaaaa tcggcgctc 
      gaagctcaa ccaggagat caacaagga gttgaggct 
      gagagctgg agccgagaa tttattcaa agctacgac 
      gaatcgcaa gttgaagga aactgtggc tttggagct 
      cagtttcgt caactccaa cctccagct gctgaagga 
      gcagctggc ggagttgaa ctcgtcggt ggagctgta 
      ccaaagcga cagcaccga cgctgtcat gcccatgat 
      cccccttgg cctgaaaga gacaaaaga gatcgactt 
      ccgggaccc cttcaagga cttcatcct ggagcacta 
      cagcgaaga cgccaacca atacgaaga tgccatagc 
      cgacttcat ggacacgcg ccaggccat aaggacacc 
      cttgcggga caccaccgg catatccct gctgttccg 
      ctactacaa ccagttgta tttcgttga gagacgttt 
      tttcccgcc tgacagatc cctcgggat ctacttcga 
      gtggttcga ttcgctaac gggagtgcc atcgtgtca 
      aaggactgt agctttcga aaaagcgtg cgttttgtt 
      caatatggg cgcgattta cacgcaaat tggtgctaa 
      acaggaccg ttccacggc gaagggatt ggatgcagc 
      cgttgatag ttttctaag ggctgcagg gacgtttcg 
      atacattca tgaaaattt cacaaatgc gccgtcgat 
      ggacttggg ccctgaaat gctggaaat gctcgtgca 
      actcatgct ggcccaagc ccgcgaatg cctcctcga 
      aaagctcga cctgcagtc aaaagaaag ccgctcgat 
      cgacatctg cctcgacct tgcccaaga agctgccca 
      tctggcaga ctgctacag ccaggtcta tcaactcat 
      aatgaccga atccgtcca cgactacgt cccctacag 
      ctggatctc tttagtcca agtaaaaaa ggaatatta 
      caccggcat ggcccatta ccatgttgc ttccggcgt 
      actgcacaa agaagcggc aaaaatgtc acccaccac 
      caaaaccac cctccagtt tttacacgc ggaaacggc 
      ctccacaca gctcgacat caggttacc gaaagacga 
      cgccgaacg aagactttt aggtcgcgc gcacttgag 
      agagtccct agtcctgca cgaggagtg ccagaggtt 
      gcaccggat gtgtcgcga gctgagagg gaagcaggc 
      ccttacagc ggtcctaag aaatgccca caaaacggc 
      ccttcaggc ctacacgaa caacgagaa tgaggacga 
      cttcagcga tgttctgga cccgccccg ggtgcaagc 
      tgcgaccaa attccaact ggctttgac gccccctga 
      cttcgccca gtaccgcgt caacgacct tttccgggc 
      tttgggccc cattgcgat tttctcggc gaaaaggca 
      ctggacggc gccccgtct cgtgcaact acaccgtgg 
      ccgaacctc tgaaggttt cggcttttc agtccgagg 
      agacgcccc agtcattgt tgcaatagt tgaatcaaa 
      ttcacttgc agagttcgg cggcgtcaa agagggtga 
      cttcatcgt ggcaattag cgataaaga tgttaaatg 
      gtcctcgca cgatgaagt cgtggcgct tatcaaaaa 
      cgccggcga ttccttaag tctcaagct ggtcactcc 
      gatggacag gaattatct taaaccgac aaagtcgac 
      aaaagggtc catttcgac gcatagtag tagttcggg 
      ggtttctag cgggatgtc tagtccgtc agggtccat 
      ggcacagca caaaaataa taaaaggtt ggggtggat 
      tccgtttaa acggcattc ctcgtcaag ggagggcaa 
      agatttttt cgagaatgt tattttgag atgaattat 
      tgtgtagtt atatcgact ttatgtcac tgtactgat 
      acgagattt atttatttt tttatcgta actacggat 
      aagtaaaat gaatgattg agatgtcgt aatgtgtac 
      attaacatt cttaaacat tattattta tagtcggca 
      ctgtacaac acaattttt attatttat gtattagag 
      atttatatt gttatttgt ttgtacatt gtatttatg 
      aatgatgtc tacgataga acccaaata aaaactata 
      ttcaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY