Status
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Gene information
General information
TC009345 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 630625..635124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009345
    iBeetle screen iB_04621

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009345 RA
      MHYFILIIS ILHGVSSLE RVSTNLRKK LDAEEQNAI 
      CCMHHMDCC REAQKPTSF EVKSMMTEC PNQNCLNWS 
      NAHQVIVQR NKTTPEKLT DCKQKLLLT IKVTNEGIP 
      SDKSEYVII DHITDPTTL QKVRILNPY AIRIRQQPM 
      LQAYQLKFE RAVNGEAKE QVFNKHTRN YRGCDTSYE 
      SPTCGLVQH EGKMVPYST GFCCSCDAE KNRQLEEHG 
      SHVPLVQWP LRDGVPDYR KNRPEENDY EHYKHYGFI 
      RPDGKMVYG RPQKRADKS VNYQQFTEL KDGKIRQRR 
      GGQTCDDAD LNIPESFRE STHCLTFSN MWYSVYQIS 
      KPEIIHKLR IQIFQKYED CHGNTHWMD ITQGKTIEL 
      GTQTPLYVE KDIIAKYCS EDIDFQDQA LDYKNLKLL 
      IPERRVVDP EQFMLLPKN SVSDGRTCD TAGVGYEAF 
      FKQRKRCAQ PQGSCLGNQ PNQLHESDA EAVKQGRVG 
      QYFLKFYGT LAEDPVGYN SSGHIQYLK MVYTKRHVS 
      TLSFELNAD LVTLLKPNS FATITEAYT DATDPSKAK 
      IIVKVTNSG LLYGVFYVR MSGCPLEVA ATFNNIASK 
      SVLIPPQHQ HIFNLLVEY PLPMKEFYC SIHVHNINQ 
      ELIAVRQVR IMTYDRCIC IWHCECACY VADKGLKCL 
      PMTLDNYHA AGFQGGFPT ETHFVETSY LDEMLAMMF 
      NMFFFLVLT LLAMGLVKA LLGLCSQEI SLWGLDTIL 
      GTESSVKKC GDKKCEYKN NSGISLAGQ FCLNVMFFF 
      LYPLALFNL CIRHYCFSA YTIEDDECS CEERWVNYE 
      CDDEVCRAK STKSSINTD QKSNGEKSS EGQPSRSNE 
      DTAKEISSE DKDLPPSGS KEEKSNEDE GKDVSSDDK 
      PEARPSKGK KQIPEDSTS EEANAKPRK GKKPLSGED 
      TASDENTRP RKGKKQKDI DEVEDEGKP DRRPRRNKG 
      KSSGESADE SSGEEKTGA RRPKKGRGR R
    CDS sequence
    • >TC009345 RA
      atgcattat ttcatcttg attatttcc attctacat 
      ggtgtgagt tcactcgaa cgcgtcagc accaattta 
      cgtaaaaaa ctcgacgcc gaagaacaa aacgccata 
      tgttgcatg caccacatg gattgctgt agagaggca 
      cagaagccg acaagtttt gaggtcaaa tctatgatg 
      accgaatgt ccgaaccaa aactgtctt aactggtca 
      aatgcccat caagtcatc gtccaacgc aacaaaacc 
      acgcctgaa aagttaaca gactgcaag caaaagcta 
      ctcctcact atcaaagtc accaacgaa ggtattccc 
      agcgataag agcgagtat gtcattatc gaccacatc 
      accgacccc acaactttg caaaaagtg cgcattttg 
      aacccttac gctatcagg atcaggcag cagccaatg 
      ctgcaagcg taccaactt aaattcgag cgagctgta 
      aacggggaa gccaaggag caagtcttt aataaacac 
      actcgtaac taccgagga tgtgacact tcctacgaa 
      agccccacc tgcggtcta gttcagcac gaaggcaaa 
      atggttccg tacagcacg ggattttgt tgctcttgc 
      gatgctgaa aaaaatcgc cagttggag gaacacggg 
      agtcatgtg cccttagtt caatggcct ctccgtgat 
      ggtgtacct gactatcgt aaaaatcgt cccgaagaa 
      aatgattat gaacattat aaacattat ggatttata 
      aggcccgat ggtaaaatg gtttatgga cggcctcaa 
      aagcgtgcc gacaaaagt gtgaattac caacaattt 
      actgagtta aaggatggt aagattcgg cagcgccgc 
      ggcggccaa acctgcgac gacgccgac ctgaacatc 
      cccgaatcc ttccgtgag agcacccat tgcctcacc 
      ttctctaac atgtggtac tccgtctac caaatctca 
      aagccggaa atcatccac aaactgcga atccaaatc 
      ttccaaaaa tacgaagac tgccacgga aacacccat 
      tggatggac ataacgcaa ggaaaaacc atagaactc 
      ggcacccaa accccgctc tacgtcgag aaagacatc 
      attgccaaa tactgctcc gaagacatt gacttccaa 
      gaccaagca cttgattac aaaaacttg aagctactg 
      atacccgaa cgccgagtc gtcgatcct gaacagttc 
      atgctctta ccgaaaaat agtgtttcg gatggtcgg 
      acgtgcgat acagctggg gtgggatat gaggcgttt 
      ttcaaacag aggaaacgt tgtgcccaa ccacagggt 
      tcctgtttg gggaaccaa ccaaatcaa ctgcatgag 
      agtgatgcc gaagctgtg aaacaggga cgtgtgggg 
      cagtatttt ttaaagttt tacgggact ttggccgag 
      gatcccgta ggttataac tcgtctgga cacattcaa 
      tacttgaaa atggtttat accaagaga catgtgagc 
      acgttgagt ttcgaattg aatgcagat ttggttacg 
      ttgttgaaa cctaactct tttgccaca atcactgaa 
      gcttacacc gatgccact gatccgtcc aaggccaaa 
      attatcgtc aaagtcaca aattctggt cttttgtat 
      ggcgttttc tatgtgaga atgagcggt tgtcccttg 
      gaagttgcc gcaactttt aacaacatt gcctccaag 
      tctgtgcta attccgcct caacaccaa cacattttc 
      aacttgtta gtggaatat ccgctccca atgaaggaa 
      ttttactgc tcaattcat gtccacaac atcaaccag 
      gagctaata gcagttcgg caagtccgg attatgacg 
      tacgatcgt tgcatttgt atctggcat tgtgagtgc 
      gcttgttac gtggctgat aaaggtctc aagtgcctg 
      ccaatgact ctggataac tatcacgct gctggtttt 
      caaggcggt ttccctaca gagacgcac tttgttgaa 
      acctcatat ctggacgag atgttggca atgatgttc 
      aacatgttc ttcttcctg gtgttgact ctccttgct 
      atgggtctg gttaaggca cttttaggg ttgtgttcc 
      caagagatc agtctttgg ggtctggat actatcctc 
      gggactgaa tctagcgtt aaaaagtgc ggtgacaaa 
      aagtgtgaa tataaaaat aacagtggg atttcacta 
      gcgggacag ttttgcttg aacgtgatg ttcttcttc 
      ttgtatccc ctagcactg ttcaatctg tgtatccga 
      cattactgt ttctcggct tatactatc gaagacgat 
      gagtgttcg tgtgaggag agatgggtt aattacgag 
      tgcgatgat gaagtttgc cgggcaaaa agcaccaaa 
      agttcgata aacaccgac cagaaaagt aacggggag 
      aagtcaagt gagggccaa ccaagcaga agtaacgaa 
      gatacggcg aaagaaatt tcaagtgag gataaagac 
      ttgccaccc agcggaagc aaggaagag aagagtaac 
      gaagacgag ggaaaggac gtttcgagt gatgataaa 
      ccagaggca aggccaagc aaaggcaag aaacagatt 
      cctgaagat tcaacgagt gaagaagca aatgcaaaa 
      ccacgtaaa gggaaaaaa ccattaagt ggggaagac 
      acagcaagt gatgaaaat acgagaccg agaaaaggc 
      aaaaaacag aaagatatt gacgaagtt gaagatgag 
      gggaagcca gaccgaaga ccacgacga aataagggt 
      aaaagtagc ggagaatca gcagatgaa agttcagga 
      gaggagaag acaggagca agaaggcca aaaaaaggg 
      agaggaagg cgttaa
    mRNA sequence
    • >TC009345 RA
      gcggaagtt ggaagcgtc taagctgat gcactaaac 
      gcccctcat aatttaatc tctgtacaa taagcggtc 
      acaacaaac cgattttta aattgatga caattagca 
      tgacgtctt tttgacaat ttccatttt accaaatgc 
      attatttca tcttgatta tttccattc tacatggtg 
      tgagttcac tcgaacgcg tcagcacca atttacgta 
      aaaaactcg acgccgaag aacaaaacg ccatatgtt 
      gcatgcacc acatggatt gctgtagag aggcacaga 
      agccgacaa gttttgagg tcaaatcta tgatgaccg 
      aatgtccga accaaaact gtcttaact ggtcaaatg 
      cccatcaag tcatcgtcc aacgcaaca aaaccacgc 
      ctgaaaagt taacagact gcaagcaaa agctactcc 
      tcactatca aagtcacca acgaaggta ttcccagcg 
      ataagagcg agtatgtca ttatcgacc acatcaccg 
      accccacaa ctttgcaaa aagtgcgca ttttgaacc 
      cttacgcta tcaggatca ggcagcagc caatgctgc 
      aagcgtacc aacttaaat tcgagcgag ctgtaaacg 
      gggaagcca aggagcaag tctttaata aacacactc 
      gtaactacc gaggatgtg acacttcct acgaaagcc 
      ccacctgcg gtctagttc agcacgaag gcaaaatgg 
      ttccgtaca gcacgggat tttgttgct cttgcgatg 
      ctgaaaaaa atcgccagt tggaggaac acgggagtc 
      atgtgccct tagttcaat ggcctctcc gtgatggtg 
      tacctgact atcgtaaaa atcgtcccg aagaaaatg 
      attatgaac attataaac attatggat ttataaggc 
      ccgatggta aaatggttt atggacggc ctcaaaagc 
      gtgccgaca aaagtgtga attaccaac aatttactg 
      agttaaagg atggtaaga ttcggcagc gccgcggcg 
      gccaaacct gcgacgacg ccgacctga acatccccg 
      aatccttcc gtgagagca cccattgcc tcaccttct 
      ctaacatgt ggtactccg tctaccaaa tctcaaagc 
      cggaaatca tccacaaac tgcgaatcc aaatcttcc 
      aaaaatacg aagactgcc acggaaaca cccattgga 
      tggacataa cgcaaggaa aaaccatag aactcggca 
      cccaaaccc cgctctacg tcgagaaag acatcattg 
      ccaaatact gctccgaag acattgact tccaagacc 
      aagcacttg attacaaaa acttgaagc tactgatac 
      ccgaacgcc gagtcgtcg atcctgaac agttcatgc 
      tcttaccga aaaatagtg tttcggatg gtcggacgt 
      gcgatacag ctggggtgg gatatgagg cgtttttca 
      aacagagga aacgttgtg cccaaccac agggttcct 
      gtttgggga accaaccaa atcaactgc atgagagtg 
      atgccgaag ctgtgaaac agggacgtg tggggcagt 
      attttttaa agttttacg ggactttgg ccgaggatc 
      ccgtaggtt ataactcgt ctggacaca ttcaatact 
      tgaaaatgg tttatacca agagacatg tgagcacgt 
      tgagtttcg aattgaatg cagatttgg ttacgttgt 
      tgaaaccta actcttttg ccacaatca ctgaagctt 
      acaccgatg ccactgatc cgtccaagg ccaaaatta 
      tcgtcaaag tcacaaatt ctggtcttt tgtatggcg 
      ttttctatg tgagaatga gcggttgtc ccttggaag 
      ttgccgcaa cttttaaca acattgcct ccaagtctg 
      tgctaattc cgcctcaac accaacaca ttttcaact 
      tgttagtgg aatatccgc tcccaatga aggaatttt 
      actgctcaa ttcatgtcc acaacatca accaggagc 
      taatagcag ttcggcaag tccggatta tgacgtacg 
      atcgttgca tttgtatct ggcattgtg agtgcgctt 
      gttacgtgg ctgataaag gtctcaagt gcctgccaa 
      tgactctgg ataactatc acgctgctg gttttcaag 
      gcggtttcc ctacagaga cgcactttg ttgaaacct 
      catatctgg acgagatgt tggcaatga tgttcaaca 
      tgttcttct tcctggtgt tgactctcc ttgctatgg 
      gtctggtta aggcacttt tagggttgt gttcccaag 
      agatcagtc tttggggtc tggatacta tcctcggga 
      ctgaatcta gcgttaaaa agtgcggtg acaaaaagt 
      gtgaatata aaaataaca gtgggattt cactagcgg 
      gacagtttt gcttgaacg tgatgttct tcttcttgt 
      atcccctag cactgttca atctgtgta tccgacatt 
      actgtttct cggcttata ctatcgaag acgatgagt 
      gttcgtgtg aggagagat gggttaatt acgagtgcg 
      atgatgaag tttgccggg caaaaagca ccaaaagtt 
      cgataaaca ccgaccaga aaagtaacg gggagaagt 
      caagtgagg gccaaccaa gcagaagta acgaagata 
      cggcgaaag aaatttcaa gtgaggata aagacttgc 
      cacccagcg gaagcaagg aagagaaga gtaacgaag 
      acgagggaa aggacgttt cgagtgatg ataaaccag 
      aggcaaggc caagcaaag gcaagaaac agattcctg 
      aagattcaa cgagtgaag aagcaaatg caaaaccac 
      gtaaaggga aaaaaccat taagtgggg aagacacag 
      caagtgatg aaaatacga gaccgagaa aaggcaaaa 
      aacagaaag atattgacg aagttgaag atgagggga 
      agccagacc gaagaccac gacgaaata agggtaaaa 
      gtagcggag aatcagcag atgaaagtt caggagagg 
      agaagacag gagcaagaa ggccaaaaa aagggagag 
      gaaggcgtt aatttactc ccaactgtg accatatct 
      ctatagatt ctgtacaat ttataataa actcttatt 
      ttttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY