Status
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Gene information
General information
TC009330 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 444685..461674
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009330
    iBeetle screen iB_04620

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009330 RA
      MTPNNTIAC SPGLMLPCW EPLVDVPLV DKVGRGLVY 
      FLSMVYLFI GVSIISDRF MAAIEVITS QEREVTIKR 
      PGGERHIIV VRMWNETVA NLTLMALGS SAPEILLSV 
      IEIYAKNFE AGELGPGTI VGSAAYNLF VIIALCVSV 
      IPDGEVRKI KHLSVFFVT AIWSVFAYV WLYMILALF 
      SPGVVEIWE GLLTFMFFP LTVLTAYAA DRKMYKYVK 
      KDYRMNRRG VIVQAEGDN LEMGSTELL GLDDVHDDI 
      KQEYITTLK ELRQQHPHA DLGTLERMA HEILLNRGP 
      KSRAFYRIQ ATRKLMGSG DLLRRISER AQSDLSEIK 
      AELQRESGA AEFNASNCR VFFEPSKYT VLESCGKFE 
      VRIARTGDL DNAVSVDYL TEDGTAQAG SDYIPIKGT 
      LHFGPGDKE KKLTLEVID DDVFEPDEH FYVKLGAAK 
      PPEVLASPT LATVIILDD DHGGLFKFE EQNHELVES 
      VGTYELKVV RCSGARGRV IVPFWTEDG TAKAGKEYE 
      GNEGQLIFE NNESEKTIP LTIIEEDSY EKDVLFYVQ 
      LGEPQMSGD EIASLAAEA EKKRPEERT EREKVAIMG 
      KPRLGNIVR AQIRIKESK EFRNTVDKL VQRANASLL 
      IGTSSWKEQ FIEAITVSA SDDSDQKKP SGLDYIMHV 
      VTLFWKILF AFVPPTDLC KGYLCFFVS ILCIGVVTA 
      VIGDVASHF GATLGIADS VTAVVFVAL GTSVPDTFA 
      SKVAAIHDK HADASIGNV TGSNAVNVF LGIGVAWSI 
      AAIYHATKG VDFRVKPGN LAFSVTIFC SEAVITIIV 
      LLLRRSRMV GGELGGPKC LKYLTSAFL LSLWIFYLI 
      MTTLEVYHV IKGF
    CDS sequence
    • >TC009330 RA
      atgactccc aacaacaca atcgcgtgc agccccggc 
      ctcatgctg ccctgctgg gagcccctg gtggacgtc 
      cccctggtc gacaaggtg ggccgcggc ctcgtctac 
      ttcctctcc atggtctac ctcttcatc ggggtgtcg 
      atcatctcg gaccgcttc atggcagcc atcgaggtc 
      atcacctcc caggagcgc gaagtcacc atcaagcgc 
      cctgggggc gagcgccac atcatcgtc gtgcggatg 
      tggaacgag accgtcgcc aatttgacc ttgatggca 
      ctaggttcc agtgcgccg gaaatcctc ctctcagtc 
      atcgaaatc tacgccaag aactttgag gctggcgag 
      ctggggccg gggaccatt gtgggctcg gcggcttac 
      aatctcttc gtgattatt gcgttgtgt gtgtcggtg 
      attccggat ggagaagtg aggaaaatc aaacacttg 
      agtgttttt ttcgtgacg gcgatttgg tccgttttt 
      gcctatgtt tggctctat atgattctg gcgctcttt 
      tctccgggg gttgtggag atttgggag ggacttttg 
      acgtttatg ttctttcca ctgacggtg cttacagcc 
      tatgcggcc gatcggaaa atgtacaaa tatgtcaag 
      aaggattat agaatgaac cgccgtggg gtgattgtc 
      caagccgaa ggcgacaac ctggagatg ggcagcact 
      gagctcctc ggcctggac gacgtccac gacgacatc 
      aagcaagag tacatcacc accctcaaa gagctgcgc 
      caacagcac ccccatgcc gacctcggc accctcgaa 
      cgcatggcc cacgagatc ctcctcaac cggggcccg 
      aaatcgcgc gctttctac cgcatccaa gccaccagg 
      aagctgatg ggcagtggg gacctcctc cgacgcatc 
      tccgaacgc gcccagagc gacctgagc gaaatcaaa 
      gcggaactc cagcgggaa tcaggcgct gccgaattc 
      aacgcctcc aactgtcgc gttttcttc gaaccttcc 
      aagtacaca gtcctggag agttgcggc aagttcgag 
      gtgcgaatt gcacggact ggggacttg gacaatgcc 
      gtcagtgtg gattatttg actgaggat gggacggca 
      caagctggg agtgattat attccgatt aaggggact 
      ttgcatttt gggcctggt gataaggag aagaagctg 
      actctggag gttattgat gatgatgtg tttgagccc 
      gatgagcat ttttatgtt aagctgggg gcggcgaag 
      ccgccggaa gtgttggct tcgcccact ttggccacg 
      gttattatt ctggatgat gatcatgga ggtttgttc 
      aagtttgag gaacagaat catgagttg gtggaaagt 
      gtggggact tatgaactg aaagtggtt aggtgttcg 
      ggggcgcga gggagggtg attgtgccg ttttggacg 
      gaggatggg acggctaag gcggggaag gagtacgaa 
      gggaatgag gggcagctt atctttgaa aacaacgaa 
      tcagagaag accatcccg ttaacaatc atcgaagaa 
      gacagctac gaaaaagac gtcctgttc tatgtacag 
      ttgggcgag cctcagatg tctggtgat gagatagca 
      agtttggca gctgaggct gaaaagaaa cgtcctgaa 
      gaacgcacc gaaagggag aaagtagcg ataatgggc 
      aaaccacga cttggaaac atcgtaaga gcacaaata 
      cgcatcaaa gagagcaag gaattccga aatactgta 
      gacaaattg gtgcaaaga gcgaacgct tctttgcta 
      attgggacg tcgtcttgg aaggagcag ttcattgag 
      gccataaca gtgagcgct agcgacgat tccgaccag 
      aaaaaaccg tcaggactg gactacatc atgcatgtt 
      gtcactctc ttctggaaa attttattc gcatttgtg 
      ccacctaca gatttatgc aagggatat ctctgcttt 
      ttcgtatcg attttgtgc attggagtg gttacggct 
      gttataggg gacgtggct tcgcatttt ggggccact 
      ctgggcatt gcagattcc gtaacagct gtggtgttt 
      gtagcgctg ggaaccagt gttccagac acatttgcc 
      agtaaagtg gcagccatc catgataag catgccgat 
      gccagtatt ggaaacgtt acaggaagt aacgctgtg 
      aatgtgttc cttggtatt ggcgtagct tggtctatt 
      gctgccatt taccacgcc accaaaggt gtggacttc 
      agagtcaaa ccggggaat ttggctttt tccgtgacg 
      attttttgt tcggaggct gttattaca ataatcgtc 
      ctgttgctg cgacgatct cgcatggtt gggggcgaa 
      ctgggagga ccaaagtgc ctcaagtat ctcactagt 
      gcttttctc ctctctcta tggattttc tacctgatt 
      atgacaacg ttggaggtc taccatgtc attaaaggg 
      ttttaa
    mRNA sequence
    • >TC009330 RA
      tccgctggc gacgtgatg caccttcac agcgcctaa 
      gatgactcc caacaacac aatcgcgtg cagccccgg 
      cctcatgct gccctgctg ggagcccct ggtggacgt 
      ccccctggt cgacaaggt gggccgcgg cctcgtcta 
      cttcctctc catggtcta cctcttcat cggggtgtc 
      gatcatctc ggaccgctt catggcagc catcgaggt 
      catcacctc ccaggagcg cgaagtcac catcaagcg 
      ccctggggg cgagcgcca catcatcgt cgtgcggat 
      gtggaacga gaccgtcgc caatttgac cttgatggc 
      actaggttc cagtgcgcc ggaaatcct cctctcagt 
      catcgaaat ctacgccaa gaactttga ggctggcga 
      gctggggcc ggggaccat tgtgggctc ggcggctta 
      caatctctt cgtgattat tgcgttgtg tgtgtcggt 
      gattccgga tggagaagt gaggaaaat caaacactt 
      gagtgtttt tttcgtgac ggcgatttg gtccgtttt 
      tgcctatgt ttggctcta tatgattct ggcgctctt 
      ttctccggg ggttgtgga gatttggga gggactttt 
      gacgtttat gttctttcc actgacggt gcttacagc 
      ctatgcggc cgatcggaa aatgtacaa atatgtcaa 
      gaaggatta tagaatgaa ccgccgtgg ggtgattgt 
      ccaagccga aggcgacaa cctggagat gggcagcac 
      tgagctcct cggcctgga cgacgtcca cgacgacat 
      caagcaaga gtacatcac caccctcaa agagctgcg 
      ccaacagca cccccatgc cgacctcgg caccctcga 
      acgcatggc ccacgagat cctcctcaa ccggggccc 
      gaaatcgcg cgctttcta ccgcatcca agccaccag 
      gaagctgat gggcagtgg ggacctcct ccgacgcat 
      ctccgaacg cgcccagag cgacctgag cgaaatcaa 
      agcggaact ccagcggga atcaggcgc tgccgaatt 
      caacgcctc caactgtcg cgttttctt cgaaccttc 
      caagtacac agtcctgga gagttgcgg caagttcga 
      ggtgcgaat tgcacggac tggggactt ggacaatgc 
      cgtcagtgt ggattattt gactgagga tgggacggc 
      acaagctgg gagtgatta tattccgat taaggggac 
      tttgcattt tgggcctgg tgataagga gaagaagct 
      gactctgga ggttattga tgatgatgt gtttgagcc 
      cgatgagca tttttatgt taagctggg ggcggcgaa 
      gccgccgga agtgttggc ttcgcccac tttggccac 
      ggttattat tctggatga tgatcatgg aggtttgtt 
      caagtttga ggaacagaa tcatgagtt ggtggaaag 
      tgtggggac ttatgaact gaaagtggt taggtgttc 
      gggggcgcg agggagggt gattgtgcc gttttggac 
      ggaggatgg gacggctaa ggcggggaa ggagtacga 
      agggaatga ggggcagct tatctttga aaacaacga 
      atcagagaa gaccatccc gttaacaat catcgaaga 
      agacagcta cgaaaaaga cgtcctgtt ctatgtaca 
      gttgggcga gcctcagat gtctggtga tgagatagc 
      aagtttggc agctgaggc tgaaaagaa acgtcctga 
      agaacgcac cgaaaggga gaaagtagc gataatggg 
      caaaccacg acttggaaa catcgtaag agcacaaat 
      acgcatcaa agagagcaa ggaattccg aaatactgt 
      agacaaatt ggtgcaaag agcgaacgc ttctttgct 
      aattgggac gtcgtcttg gaaggagca gttcattga 
      ggccataac agtgagcgc tagcgacga ttccgacca 
      gaaaaaacc gtcaggact ggactacat catgcatgt 
      tgtcactct cttctggaa aattttatt cgcatttgt 
      gccacctac agatttatg caagggata tctctgctt 
      tttcgtatc gattttgtg cattggagt ggttacggc 
      tgttatagg ggacgtggc ttcgcattt tggggccac 
      tctgggcat tgcagattc cgtaacagc tgtggtgtt 
      tgtagcgct gggaaccag tgttccaga cacatttgc 
      cagtaaagt ggcagccat ccatgataa gcatgccga 
      tgccagtat tggaaacgt tacaggaag taacgctgt 
      gaatgtgtt ccttggtat tggcgtagc ttggtctat 
      tgctgccat ttaccacgc caccaaagg tgtggactt 
      cagagtcaa accggggaa tttggcttt ttccgtgac 
      gattttttg ttcggaggc tgttattac aataatcgt 
      cctgttgct gcgacgatc tcgcatggt tgggggcga 
      actgggagg accaaagtg cctcaagta tctcactag 
      tgcttttct cctctctct atggatttt ctacctgat 
      tatgacaac gttggaggt ctaccatgt cattaaagg 
      gttttaaga ttcttacaa tgtacttat ataattact 
      tctctatat tcgtcaaca acgcaatgt tacttaaat 
      cacaccagt gatactcaa tagtccact cacgttatg 
      tcgcttatc gattttgtg atcgccctc tcgtggcta 
      acaagtaga cttaattta tgccttaag caagacagg 
      cctgccaat agtacgtta tatttttgc gacatgagt 
      tacagacca gtctaacaa aaaccacaa aaaaaatct 
      gatcaattt ttcctgctg tagtaatta taaaaaatg 
      tatcttttc agcttaaac ttttttccc tacagtttt 
      tttgctgga ttggtctat gatcgtact atacaggtg 
      gtccagtcc agctcccgt cttctgtag cccagttat 
      ctatttttt tggttttta gttattttt aagtaattc 
      ctgaaccga tttgaaaaa actttaggg acatgtaag 
      aagctaaaa aagtgtcct aacgaccac aaaaaataa 
      tagcttgtt gttaatttt ttttattat gacatcata 
      tttttggga ccctagacc ctttactaa taagttaac 
      cgagttgcg gaaggtggg agctgagct ggaccagcc 
      agtatatgt ataattttt tttattacg cactggaaa 
      ttaatggat aatgttaac tagagttag tctgactta 
      gccttagag tatgcgagt gttcaacaa cataacttt 
      aataattct agtgttgtg agttgcact gttctattt 
      gtgtaactt cccgaattc ggtggcttc gtttcagac 
      gtccagaaa tgtcgaagt gccacattt ggggcgttc 
      ttagtcata attattcta gtcatgttc tagtgatgt 
      gcaattcaa aaagaccag aaagcaatg ggaattcaa 
      attatacac acaaacgaa ttaaattaa atcaaaagg 
      ctgacgaaa ggaaccact ggtagatta ttctgactg 
      tgtactgta ggtaatgct atatttaaa aatgtctca 
      cacagtaaa acgaaatca aaggacata atcacacga 
      gtggatgca tttttaaaa atggcaata catccaaat 
      tagactaga acatctcag caatagctt ttgtacggc 
      ggttagcta gtcttagcg aattctata atttgattc 
      ttcccattt gaagggtaa agctggaaa aggagataa 
      atacttcca ctatagtta gtacatgtt gtttcgggt 
      tttgttgct ctgtttcca ttgagttcc aacgattta 
      atcaaaaca ctaaaacta acttaaaat tgttggaat 
      tctgctacg tgttctgtg ttgtctgta ggtattgtt 
      tttggtgaa acggattaa ctgaattca ctccaatcc 
      cgatgaaat gtgttatgg gcttccaat agttgttta 
      tttatccac gacgtttga tttatttca caaattaga 
      ggaaccttc tgcagttac gataattta aacaaatga 
      cggaaatcc tatttaagt aataataga cctaataat 
      taaattgcc ttaccccca gaaatgaaa acattttac 
      gttaagtgt ttgtaatag aatagacat ggtctcata 
      taagagtaa tgttaggtg ttgataaat ttgtattat 
      ttttttatg attttattg tagtttagg taagtacat 
      atctttggc acaagagtg caagccaaa tgaaccaaa 
      aatattata gaatggata ttatgctaa agttgttgt 
      cattatagt taagtttaa gcaaagaat gttcgattt 
      aataaaaat gtacctagt taaaggcat ttatttcat 
      taccacttt gtccctctt cacattata atttattga 
      taaaattta ctttgaaaa aaaaatgtt ttaagagtt 
      ttacaaatt aacgactaa taatttcaa gtaaaaaca
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