| General information | |||
| TC009330 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 444685..461674 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009330 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04620 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC009330 RA
MTPNNTIAC SPGLMLPCW EPLVDVPLV DKVGRGLVY FLSMVYLFI GVSIISDRF MAAIEVITS QEREVTIKR PGGERHIIV VRMWNETVA NLTLMALGS SAPEILLSV IEIYAKNFE AGELGPGTI VGSAAYNLF VIIALCVSV IPDGEVRKI KHLSVFFVT AIWSVFAYV WLYMILALF SPGVVEIWE GLLTFMFFP LTVLTAYAA DRKMYKYVK KDYRMNRRG VIVQAEGDN LEMGSTELL GLDDVHDDI KQEYITTLK ELRQQHPHA DLGTLERMA HEILLNRGP KSRAFYRIQ ATRKLMGSG DLLRRISER AQSDLSEIK AELQRESGA AEFNASNCR VFFEPSKYT VLESCGKFE VRIARTGDL DNAVSVDYL TEDGTAQAG SDYIPIKGT LHFGPGDKE KKLTLEVID DDVFEPDEH FYVKLGAAK PPEVLASPT LATVIILDD DHGGLFKFE EQNHELVES VGTYELKVV RCSGARGRV IVPFWTEDG TAKAGKEYE GNEGQLIFE NNESEKTIP LTIIEEDSY EKDVLFYVQ LGEPQMSGD EIASLAAEA EKKRPEERT EREKVAIMG KPRLGNIVR AQIRIKESK EFRNTVDKL VQRANASLL IGTSSWKEQ FIEAITVSA SDDSDQKKP SGLDYIMHV VTLFWKILF AFVPPTDLC KGYLCFFVS ILCIGVVTA VIGDVASHF GATLGIADS VTAVVFVAL GTSVPDTFA SKVAAIHDK HADASIGNV TGSNAVNVF LGIGVAWSI AAIYHATKG VDFRVKPGN LAFSVTIFC SEAVITIIV LLLRRSRMV GGELGGPKC LKYLTSAFL LSLWIFYLI MTTLEVYHV IKGF
- >TC009330 RA
atgactccc aacaacaca atcgcgtgc agccccggc ctcatgctg ccctgctgg gagcccctg gtggacgtc cccctggtc gacaaggtg ggccgcggc ctcgtctac ttcctctcc atggtctac ctcttcatc ggggtgtcg atcatctcg gaccgcttc atggcagcc atcgaggtc atcacctcc caggagcgc gaagtcacc atcaagcgc cctgggggc gagcgccac atcatcgtc gtgcggatg tggaacgag accgtcgcc aatttgacc ttgatggca ctaggttcc agtgcgccg gaaatcctc ctctcagtc atcgaaatc tacgccaag aactttgag gctggcgag ctggggccg gggaccatt gtgggctcg gcggcttac aatctcttc gtgattatt gcgttgtgt gtgtcggtg attccggat ggagaagtg aggaaaatc aaacacttg agtgttttt ttcgtgacg gcgatttgg tccgttttt gcctatgtt tggctctat atgattctg gcgctcttt tctccgggg gttgtggag atttgggag ggacttttg acgtttatg ttctttcca ctgacggtg cttacagcc tatgcggcc gatcggaaa atgtacaaa tatgtcaag aaggattat agaatgaac cgccgtggg gtgattgtc caagccgaa ggcgacaac ctggagatg ggcagcact gagctcctc ggcctggac gacgtccac gacgacatc aagcaagag tacatcacc accctcaaa gagctgcgc caacagcac ccccatgcc gacctcggc accctcgaa cgcatggcc cacgagatc ctcctcaac cggggcccg aaatcgcgc gctttctac cgcatccaa gccaccagg aagctgatg ggcagtggg gacctcctc cgacgcatc tccgaacgc gcccagagc gacctgagc gaaatcaaa gcggaactc cagcgggaa tcaggcgct gccgaattc aacgcctcc aactgtcgc gttttcttc gaaccttcc aagtacaca gtcctggag agttgcggc aagttcgag gtgcgaatt gcacggact ggggacttg gacaatgcc gtcagtgtg gattatttg actgaggat gggacggca caagctggg agtgattat attccgatt aaggggact ttgcatttt gggcctggt gataaggag aagaagctg actctggag gttattgat gatgatgtg tttgagccc gatgagcat ttttatgtt aagctgggg gcggcgaag ccgccggaa gtgttggct tcgcccact ttggccacg gttattatt ctggatgat gatcatgga ggtttgttc aagtttgag gaacagaat catgagttg gtggaaagt gtggggact tatgaactg aaagtggtt aggtgttcg ggggcgcga gggagggtg attgtgccg ttttggacg gaggatggg acggctaag gcggggaag gagtacgaa gggaatgag gggcagctt atctttgaa aacaacgaa tcagagaag accatcccg ttaacaatc atcgaagaa gacagctac gaaaaagac gtcctgttc tatgtacag ttgggcgag cctcagatg tctggtgat gagatagca agtttggca gctgaggct gaaaagaaa cgtcctgaa gaacgcacc gaaagggag aaagtagcg ataatgggc aaaccacga cttggaaac atcgtaaga gcacaaata cgcatcaaa gagagcaag gaattccga aatactgta gacaaattg gtgcaaaga gcgaacgct tctttgcta attgggacg tcgtcttgg aaggagcag ttcattgag gccataaca gtgagcgct agcgacgat tccgaccag aaaaaaccg tcaggactg gactacatc atgcatgtt gtcactctc ttctggaaa attttattc gcatttgtg ccacctaca gatttatgc aagggatat ctctgcttt ttcgtatcg attttgtgc attggagtg gttacggct gttataggg gacgtggct tcgcatttt ggggccact ctgggcatt gcagattcc gtaacagct gtggtgttt gtagcgctg ggaaccagt gttccagac acatttgcc agtaaagtg gcagccatc catgataag catgccgat gccagtatt ggaaacgtt acaggaagt aacgctgtg aatgtgttc cttggtatt ggcgtagct tggtctatt gctgccatt taccacgcc accaaaggt gtggacttc agagtcaaa ccggggaat ttggctttt tccgtgacg attttttgt tcggaggct gttattaca ataatcgtc ctgttgctg cgacgatct cgcatggtt gggggcgaa ctgggagga ccaaagtgc ctcaagtat ctcactagt gcttttctc ctctctcta tggattttc tacctgatt atgacaacg ttggaggtc taccatgtc attaaaggg ttttaa
- >TC009330 RA
tccgctggc gacgtgatg caccttcac agcgcctaa gatgactcc caacaacac aatcgcgtg cagccccgg cctcatgct gccctgctg ggagcccct ggtggacgt ccccctggt cgacaaggt gggccgcgg cctcgtcta cttcctctc catggtcta cctcttcat cggggtgtc gatcatctc ggaccgctt catggcagc catcgaggt catcacctc ccaggagcg cgaagtcac catcaagcg ccctggggg cgagcgcca catcatcgt cgtgcggat gtggaacga gaccgtcgc caatttgac cttgatggc actaggttc cagtgcgcc ggaaatcct cctctcagt catcgaaat ctacgccaa gaactttga ggctggcga gctggggcc ggggaccat tgtgggctc ggcggctta caatctctt cgtgattat tgcgttgtg tgtgtcggt gattccgga tggagaagt gaggaaaat caaacactt gagtgtttt tttcgtgac ggcgatttg gtccgtttt tgcctatgt ttggctcta tatgattct ggcgctctt ttctccggg ggttgtgga gatttggga gggactttt gacgtttat gttctttcc actgacggt gcttacagc ctatgcggc cgatcggaa aatgtacaa atatgtcaa gaaggatta tagaatgaa ccgccgtgg ggtgattgt ccaagccga aggcgacaa cctggagat gggcagcac tgagctcct cggcctgga cgacgtcca cgacgacat caagcaaga gtacatcac caccctcaa agagctgcg ccaacagca cccccatgc cgacctcgg caccctcga acgcatggc ccacgagat cctcctcaa ccggggccc gaaatcgcg cgctttcta ccgcatcca agccaccag gaagctgat gggcagtgg ggacctcct ccgacgcat ctccgaacg cgcccagag cgacctgag cgaaatcaa agcggaact ccagcggga atcaggcgc tgccgaatt caacgcctc caactgtcg cgttttctt cgaaccttc caagtacac agtcctgga gagttgcgg caagttcga ggtgcgaat tgcacggac tggggactt ggacaatgc cgtcagtgt ggattattt gactgagga tgggacggc acaagctgg gagtgatta tattccgat taaggggac tttgcattt tgggcctgg tgataagga gaagaagct gactctgga ggttattga tgatgatgt gtttgagcc cgatgagca tttttatgt taagctggg ggcggcgaa gccgccgga agtgttggc ttcgcccac tttggccac ggttattat tctggatga tgatcatgg aggtttgtt caagtttga ggaacagaa tcatgagtt ggtggaaag tgtggggac ttatgaact gaaagtggt taggtgttc gggggcgcg agggagggt gattgtgcc gttttggac ggaggatgg gacggctaa ggcggggaa ggagtacga agggaatga ggggcagct tatctttga aaacaacga atcagagaa gaccatccc gttaacaat catcgaaga agacagcta cgaaaaaga cgtcctgtt ctatgtaca gttgggcga gcctcagat gtctggtga tgagatagc aagtttggc agctgaggc tgaaaagaa acgtcctga agaacgcac cgaaaggga gaaagtagc gataatggg caaaccacg acttggaaa catcgtaag agcacaaat acgcatcaa agagagcaa ggaattccg aaatactgt agacaaatt ggtgcaaag agcgaacgc ttctttgct aattgggac gtcgtcttg gaaggagca gttcattga ggccataac agtgagcgc tagcgacga ttccgacca gaaaaaacc gtcaggact ggactacat catgcatgt tgtcactct cttctggaa aattttatt cgcatttgt gccacctac agatttatg caagggata tctctgctt tttcgtatc gattttgtg cattggagt ggttacggc tgttatagg ggacgtggc ttcgcattt tggggccac tctgggcat tgcagattc cgtaacagc tgtggtgtt tgtagcgct gggaaccag tgttccaga cacatttgc cagtaaagt ggcagccat ccatgataa gcatgccga tgccagtat tggaaacgt tacaggaag taacgctgt gaatgtgtt ccttggtat tggcgtagc ttggtctat tgctgccat ttaccacgc caccaaagg tgtggactt cagagtcaa accggggaa tttggcttt ttccgtgac gattttttg ttcggaggc tgttattac aataatcgt cctgttgct gcgacgatc tcgcatggt tgggggcga actgggagg accaaagtg cctcaagta tctcactag tgcttttct cctctctct atggatttt ctacctgat tatgacaac gttggaggt ctaccatgt cattaaagg gttttaaga ttcttacaa tgtacttat ataattact tctctatat tcgtcaaca acgcaatgt tacttaaat cacaccagt gatactcaa tagtccact cacgttatg tcgcttatc gattttgtg atcgccctc tcgtggcta acaagtaga cttaattta tgccttaag caagacagg cctgccaat agtacgtta tatttttgc gacatgagt tacagacca gtctaacaa aaaccacaa aaaaaatct gatcaattt ttcctgctg tagtaatta taaaaaatg tatcttttc agcttaaac ttttttccc tacagtttt tttgctgga ttggtctat gatcgtact atacaggtg gtccagtcc agctcccgt cttctgtag cccagttat ctatttttt tggttttta gttattttt aagtaattc ctgaaccga tttgaaaaa actttaggg acatgtaag aagctaaaa aagtgtcct aacgaccac aaaaaataa tagcttgtt gttaatttt ttttattat gacatcata tttttggga ccctagacc ctttactaa taagttaac cgagttgcg gaaggtggg agctgagct ggaccagcc agtatatgt ataattttt tttattacg cactggaaa ttaatggat aatgttaac tagagttag tctgactta gccttagag tatgcgagt gttcaacaa cataacttt aataattct agtgttgtg agttgcact gttctattt gtgtaactt cccgaattc ggtggcttc gtttcagac gtccagaaa tgtcgaagt gccacattt ggggcgttc ttagtcata attattcta gtcatgttc tagtgatgt gcaattcaa aaagaccag aaagcaatg ggaattcaa attatacac acaaacgaa ttaaattaa atcaaaagg ctgacgaaa ggaaccact ggtagatta ttctgactg tgtactgta ggtaatgct atatttaaa aatgtctca cacagtaaa acgaaatca aaggacata atcacacga gtggatgca tttttaaaa atggcaata catccaaat tagactaga acatctcag caatagctt ttgtacggc ggttagcta gtcttagcg aattctata atttgattc ttcccattt gaagggtaa agctggaaa aggagataa atacttcca ctatagtta gtacatgtt gtttcgggt tttgttgct ctgtttcca ttgagttcc aacgattta atcaaaaca ctaaaacta acttaaaat tgttggaat tctgctacg tgttctgtg ttgtctgta ggtattgtt tttggtgaa acggattaa ctgaattca ctccaatcc cgatgaaat gtgttatgg gcttccaat agttgttta tttatccac gacgtttga tttatttca caaattaga ggaaccttc tgcagttac gataattta aacaaatga cggaaatcc tatttaagt aataataga cctaataat taaattgcc ttaccccca gaaatgaaa acattttac gttaagtgt ttgtaatag aatagacat ggtctcata taagagtaa tgttaggtg ttgataaat ttgtattat ttttttatg attttattg tagtttagg taagtacat atctttggc acaagagtg caagccaaa tgaaccaaa aatattata gaatggata ttatgctaa agttgttgt cattatagt taagtttaa gcaaagaat gttcgattt aataaaaat gtacctagt taaaggcat ttatttcat taccacttt gtccctctt cacattata atttattga taaaattta ctttgaaaa aaaaatgtt ttaagagtt ttacaaatt aacgactaa taatttcaa gtaaaaaca
