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Gene information
General information
TC009311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 231508..242535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009311
    iBeetle screen iB_04616

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009311 RA
      MKRLRVDET LKEGSVSPA TAALYSVNQ QQHQQQQST 
      TTSSSSTLS YSSASNVKV VSEGMQSGL YPPPPVSYG 
      AMPTATAPH SGKASAAMQ PLSVGGGGG GGGGGGGGG 
      HTQLRTSAG SSPPTAPPI QEMQQQSQQ QSPGSNFQR 
      LKVEDALSY LDLVKYKFG SKPQVYNDF LDIMKEFKS 
      QSIDTPGVI QRVSNLFKG FPDLIVGFN TFLPPGYKI 
      EVQKSDQGY AFQVSVSMP SPTGNMPSD PQQQKMIMR 
      GSGTINMSN SASVLNTLP PCQPLSLTQ HIPTVQAQT 
      AAPAIPPPP VPVHHSTSS YGTGAPTYN NSSMTLAAA 
      QVAVSQALQ GHTDTPQNQ PVQFNHAIN YVNKIKNRF 
      QDQPEKYKR FLDILHIYQ KEQRTMKES SGGGSGGKH 
      LTEQEVYSQ VAKLFENQS DLLAEFGQF LPDATSHIN 
      PAPISDHSV VKKPPTKQP YRENIMDRP SHKPSHHIS 
      GQLKRSPTY TPMMHRDAP PPKKHKMSS CRDVTLAEA 
      GKYGTLNDY AFFDKVRKA VRTQEVYNN FLRCLILFN 
      QEIISKSEL IMVVTPFLG KFPELMRWF REFLGQNEV 
      EPMPYNATR TERPQGETA LEIDLTTAK RLGASYCVI 
      PPSQEGLTC SGRTQLCKE VLNDQWVSF PTWSEDSTF 
      VSSRKTQYE EYMYRCEDE RFELDVVIE TNASTIRVL 
      EGVNKKMSR MNAEDVAKY RLDDCLGGS SPTLHQRAL 
      KRIYGDKAQ DIIDGLKRN PQTAVPVVL RRLKAKEEE 
      WREAQKGFN KIWREQNEK YYLKSLDHQ GINFKQTDV 
      KALRSKSLF NEIETLFDE RHEQNEEGA EPVVGPHLV 
      LPYKDRTIL DDAANLLIH HVKRQTGIQ KGEKRRIKH 
      LLRQFLPEL FFHPKQQLT DDERDDDEK EDGDDSPIS 
      SPTGNNSKG KSESSPGKS PGRLDGEVE VKTDPDCKD 
      EDIKVPLHA QVSDPDEAY TLFMANNNW YIFLRLHAV 
      LCERLAKIY ERAVILAAE EAKSRTGRK ESTAVALRL 
      KPKPQIEVE DYYVAFLDM VKNLLDGNM DANTYEDTL 
      REMFGIHAY IAFTLDKVV SYAVRQLQH CVTERTAIA 
      CTDLFVKEQ KRGATGGSC STAHKRAHL EMAYQRLGE 
      KAVQDENCF KIYIYKKDC RMTIELLDT DSEDPKKVE 
      DTKKWSSYK ECYADITHV DNKPRSPVY LNRNLRLYK 
      KRMGLHNKT SSVVEKKNK QQREENDEQ KKNQDEENK 
      KTNRATTDE ADNKFAMLE ELERKFSEC PLPERNQTE 
      HPGYEVSDE TQCKFNSQD PKIMFTVNK DSYLYKISA 
      FSRARKSHP AITKRLSCK FRAWLKHWA SENVSDVQH 
      KQCTDWLMG RGENVVPNR TRVLTDNDL SRTPYVPYN 
      RYRVERLNE DVTAT
    CDS sequence
    • >TC009311 RA
      atgaaacgg cttcgagtg gatgagacg ctgaaagaa 
      ggaagtgtg tctccagcg acagctgct ctctacagc 
      gttaatcag cagcagcac cagcagcag caatcaacg 
      acgacctcg tcgtcgtca acattgtcc tactcgagt 
      gctagtaac gtaaaagtg gtcagtgaa ggaatgcag 
      tcgggactt tatccgccg cctccggtg agttacggc 
      gcgatgcca acggcgact gcgccccac tccggcaaa 
      gcttccgca gcaatgcaa cctttatcc gtcggtggc 
      ggcggcggc ggcggcggt ggtggtggc ggcggcggc 
      catactcaa ttacgcacc agtgccggt tcatcacca 
      cccaccgcc cctcctatc caagaaatg caacagcaa 
      tctcaacaa caatcacct ggcagcaac ttccaacgt 
      ttaaaagtc gaagacgcc ctctcctat ctcgacctg 
      gtcaaatat aaattcggt tccaagcct caagtgtac 
      aacgacttt ttggacatt atgaaagag tttaagagt 
      caaagtatt gacacccca ggtgttatc caacgcgta 
      tcaaactta ttcaaaggt tttcccgat ctgattgtt 
      ggttttaat acgttttta ccgcccggt tataagatt 
      gaagtacag aaaagtgat cagggatat gcatttcaa 
      gtctctgtg agtatgcca agtccgacg ggaaatatg 
      ccctctgat cctcaacaa cagaagatg attatgagg 
      ggttccgga acgatcaac atgtcgaat tcggcgtcg 
      gtgttgaac acgctacca ccatgtcag ccactatcg 
      ttgacgcag cacatacct acggttcag gctcaaacg 
      gccgcaccg gcaatacca ccaccccca gtgcctgta 
      catcattca acgtcaagt tacggcact ggagcgccg 
      acctacaac aactcgtcg atgacactg gcggcggcg 
      caagtcgcc gtgagtcaa gcgctgcaa ggacacacg 
      gatacgccg cagaatcag ccggtgcaa ttcaatcat 
      gccattaat tacgttaat aagattaag aatcgattc 
      caagaccag ccagaaaaa tacaaacga ttcttggac 
      attttacat atataccaa aaagagcag cgcacaatg 
      aaagaatct tcaggcggt ggttcgggt ggcaaacac 
      ttaaccgaa caagaagtc tacagtcaa gtagcaaaa 
      ctttttgaa aatcagagc gatcttttg gctgaattt 
      ggtcaattc ttacctgat gcaacgagt catattaat 
      ccggcaccg atttcggat cattccgtc gtcaaaaaa 
      ccaccaact aaacaacct tacagagag aatatcatg 
      gatagacct tcacacaag ccgagtcat catatttcg 
      ggacaattg aaacgttca cctacttat actccaatg 
      atgcataga gatgcacca ccgccgaaa aaacataaa 
      atgtcatca tgtcgggat gtgacatta gccgaggcg 
      ggaaaatat gggacatta aacgattat gcctttttt 
      gataaggtg cgaaaagcg gtacgcacc caagaagtc 
      tacaataat ttcctgcgc tgtttgata ctcttcaat 
      caggagata atctcaaaa agtgaatta ataatggtc 
      gtgactcca tttttgggt aaattcccc gaattgatg 
      cgatggttt cgtgaattt cttggtcag aacgaagtt 
      gaaccgatg ccttacaac gccacacga actgaacga 
      ccgcaaggc gaaacagca cttgaaatc gatctgacc 
      actgcgaaa cgtctaggt gcgagttat tgcgtcatt 
      ccaccgtca caggaaggt ctcacttgt tcgggtcga 
      actcagttg tgcaaggaa gttttgaat gaccaatgg 
      gtgtcattt ccaacgtgg tcggaagat agtaccttt 
      gttagttcg cgaaagaca caatatgaa gagtacatg 
      tacaggtgt gaagatgaa aggttcgag ctggacgtt 
      gtgatcgaa accaacgct tcaacaatt cgtgttctc 
      gaaggtgta aacaagaaa atgagtcgt atgaatgca 
      gaagacgtt gccaaatat cgtctggac gactgtctt 
      ggtggttca tctcctaca ttgcatcaa cgcgccttg 
      aaacgaatc tacggtgat aaagcacaa gatataatt 
      gatggcctg aaacgaaat cctcaaact gctgttccg 
      gtcgttttg cgacgactc aaagcgaaa gaagaggaa 
      tggagagaa gctcaaaaa ggttttaat aagatatgg 
      agggaacag aacgaaaaa tactactta aagagtttg 
      gatcatcaa gggatcaat ttcaaacag actgatgtt 
      aaagcgttg cgatcaaag agtttgttt aatgaaatc 
      gagacgttg tttgatgaa agacacgaa cagaatgaa 
      gaaggtgcc gagccagtg gttggacca catttagta 
      ttgccctac aaggatagg acgattttg gatgatgct 
      gcgaatcta ttgattcat catgttaaa agacagacc 
      ggaatacag aaaggggag aagaggaga attaagcat 
      ttattgaga caatttttg ccagagttg tttttccat 
      ccgaagcag cagttgacg gacgacgaa cgtgatgac 
      gatgaaaaa gaggatggt gacgacagc ccgataagc 
      agtcccact gggaacaac tctaaaggt aaaagcgaa 
      tcaagtcca ggaaaatct cccggaaga ttggacgga 
      gaagttgaa gtaaaaaca gatcctgat tgtaaagac 
      gaagacatc aaagttcct ttacatgct caagtttca 
      gatccggat gaagcttat actctattc atggccaat 
      aataattgg tacatattc cttcgcctt catgctgtt 
      ttatgcgaa cgtttggcg aaaatctac gaacgtgct 
      gtaattctg gcagctgaa gaagcaaaa tctcgaaca 
      ggtcgtaaa gaaagtaca gccgttgca cttcgattg 
      aaaccaaag cctcagatt gaagttgaa gattattac 
      gttgcattt ttggatatg gttaaaaat ctcttggat 
      ggtaatatg gacgctaat acttacgaa gatactctt 
      cgagaaatg ttcggtatt catgcttat attgcgttc 
      actttggat aaagtggtt tcgtatgcg gttcgtcaa 
      cttcagcac tgtgtgact gaacgaact gcaattgct 
      tgcactgat ttatttgtc aaggaacaa aaacgtgga 
      gctaccgga ggatcatgt tctacagcg cacaaaaga 
      gctcatcta gagatggcg tatcaaagg ctaggcgaa 
      aaagctgta caagacgag aattgtttc aagatctat 
      atctataag aaggattgt aggatgact attgagttg 
      cttgatacg gattcagag gatccgaaa aaggttgaa 
      gatacgaaa aagtggagt tcgtataaa gagtgttac 
      gcggatata acacatgtg gacaataaa ccgcgttcg 
      ccggtctat ttaaatcgg aatttaagg ttgtataaa 
      aaacgtatg gggcttcat aataaaacg tcatcggtg 
      gtggaaaaa aagaataaa caacaaagg gaagagaac 
      gacgaacaa aagaagaat caagatgaa gagaacaaa 
      aagacaaat agggcaacg accgacgaa gcggacaat 
      aaatttgca atgttggag gagttggag aggaagttt 
      agcgaatgt cctttgcct gaaagaaat caaacggaa 
      catccggga tatgaagtt tcggatgaa acgcaatgt 
      aaatttaat tcacaggat ccgaaaatt atgtttacg 
      gttaacaag gatagttac ttgtataaa ataagtgcc 
      ttttcgaga gcgagaaag agtcatcct gcgataacg 
      aaacgcttg tcatgtaaa tttcgcgcg tggctgaaa 
      cattgggca agtgagaac gttagtgac gtgcaacat 
      aaacagtgt accgattgg ttgatgggt cgcggcgaa 
      aatgtcgta cctaaccga acacgggtg ctaacagac 
      aatgatctt tcacgaact ccttatgta ccatacaac 
      agatacaga gtcgaaagg ctgaacgaa gatgttact 
      gctacgtga
    mRNA sequence
    • >TC009311 RA
      tttggcggg tatttttaa aaatcgata acagcgcca 
      ctgttctct tcacattga actatcaga gcaagcatc 
      aaacaaacc ttttacgga tcagacaat tgattcgag 
      gggcggtcg ctagtatcc gaaactgaa aaaatccac 
      aatatgact aaagtgtaa tttttctgg aattgggag 
      ctcatcctg tggcacgtc gtaatgttt gtgtgtagg 
      ttttgtgca ttttggtga tcaaaaact tgaaaaatt 
      tgaaagtgg gcaaagcga ccaaccacc cgaaattga 
      tggactcgt ccaaacaag ctcggagat ttgaagcag 
      ccagtcagt cgacaatcg gtcatcgtt tcgggtggt 
      tcggaacag aatgtgtca gaattttct tttgatgaa 
      ttctagtgt tttccaatt atttcccag tgaaatcag 
      cgcggatcg gttgcaatt gtacggtgc atccccggc 
      accagtccg cacggaagc gctttccgc gaaagaggc 
      ttttgtaag ccgcctcga tgtaatttg aaatgaaac 
      ggcttcgag tggatgaga cgctgaaag aaggaagtg 
      tgtctccag cgacagctg ctctctaca gcgttaatc 
      agcagcagc accagcagc agcaatcaa cgacgacct 
      cgtcgtcgt caacattgt cctactcga gtgctagta 
      acgtaaaag tggtcagtg aaggaatgc agtcgggac 
      tttatccgc cgcctccgg tgagttacg gcgcgatgc 
      caacggcga ctgcgcccc actccggca aagcttccg 
      cagcaatgc aacctttat ccgtcggtg gcggcggcg 
      gcggcggcg gtggtggtg gcggcggcg gccatactc 
      aattacgca ccagtgccg gttcatcac cacccaccg 
      cccctccta tccaagaaa tgcaacagc aatctcaac 
      aacaatcac ctggcagca acttccaac gtttaaaag 
      tcgaagacg ccctctcct atctcgacc tggtcaaat 
      ataaattcg gttccaagc ctcaagtgt acaacgact 
      ttttggaca ttatgaaag agtttaaga gtcaaagta 
      ttgacaccc caggtgtta tccaacgcg tatcaaact 
      tattcaaag gttttcccg atctgattg ttggtttta 
      atacgtttt taccgcccg gttataaga ttgaagtac 
      agaaaagtg atcagggat atgcatttc aagtctctg 
      tgagtatgc caagtccga cgggaaata tgccctctg 
      atcctcaac aacagaaga tgattatga ggggttccg 
      gaacgatca acatgtcga attcggcgt cggtgttga 
      acacgctac caccatgtc agccactat cgttgacgc 
      agcacatac ctacggttc aggctcaaa cggccgcac 
      cggcaatac caccacccc cagtgcctg tacatcatt 
      caacgtcaa gttacggca ctggagcgc cgacctaca 
      acaactcgt cgatgacac tggcggcgg cgcaagtcg 
      ccgtgagtc aagcgctgc aaggacaca cggatacgc 
      cgcagaatc agccggtgc aattcaatc atgccatta 
      attacgtta ataagatta agaatcgat tccaagacc 
      agccagaaa aatacaaac gattcttgg acattttac 
      atatatacc aaaaagagc agcgcacaa tgaaagaat 
      cttcaggcg gtggttcgg gtggcaaac acttaaccg 
      aacaagaag tctacagtc aagtagcaa aactttttg 
      aaaatcaga gcgatcttt tggctgaat ttggtcaat 
      tcttacctg atgcaacga gtcatatta atccggcac 
      cgatttcgg atcattccg tcgtcaaaa aaccaccaa 
      ctaaacaac cttacagag agaatatca tggatagac 
      cttcacaca agccgagtc atcatattt cgggacaat 
      tgaaacgtt cacctactt atactccaa tgatgcata 
      gagatgcac caccgccga aaaaacata aaatgtcat 
      catgtcggg atgtgacat tagccgagg cgggaaaat 
      atgggacat taaacgatt atgcctttt ttgataagg 
      tgcgaaaag cggtacgca cccaagaag tctacaata 
      atttcctgc gctgtttga tactcttca atcaggaga 
      taatctcaa aaagtgaat taataatgg tcgtgactc 
      catttttgg gtaaattcc ccgaattga tgcgatggt 
      ttcgtgaat ttcttggtc agaacgaag ttgaaccga 
      tgccttaca acgccacac gaactgaac gaccgcaag 
      gcgaaacag cacttgaaa tcgatctga ccactgcga 
      aacgtctag gtgcgagtt attgcgtca ttccaccgt 
      cacaggaag gtctcactt gttcgggtc gaactcagt 
      tgtgcaagg aagttttga atgaccaat gggtgtcat 
      ttccaacgt ggtcggaag atagtacct ttgttagtt 
      cgcgaaaga cacaatatg aagagtaca tgtacaggt 
      gtgaagatg aaaggttcg agctggacg ttgtgatcg 
      aaaccaacg cttcaacaa ttcgtgttc tcgaaggtg 
      taaacaaga aaatgagtc gtatgaatg cagaagacg 
      ttgccaaat atcgtctgg acgactgtc ttggtggtt 
      catctccta cattgcatc aacgcgcct tgaaacgaa 
      tctacggtg ataaagcac aagatataa ttgatggcc 
      tgaaacgaa atcctcaaa ctgctgttc cggtcgttt 
      tgcgacgac tcaaagcga aagaagagg aatggagag 
      aagctcaaa aaggtttta ataagatat ggagggaac 
      agaacgaaa aatactact taaagagtt tggatcatc 
      aagggatca atttcaaac agactgatg ttaaagcgt 
      tgcgatcaa agagtttgt ttaatgaaa tcgagacgt 
      tgtttgatg aaagacacg aacagaatg aagaaggtg 
      ccgagccag tggttggac cacatttag tattgccct 
      acaaggata ggacgattt tggatgatg ctgcgaatc 
      tattgattc atcatgtta aaagacaga ccggaatac 
      agaaagggg agaagagga gaattaagc atttattga 
      gacaatttt tgccagagt tgtttttcc atccgaagc 
      agcagttga cggacgacg aacgtgatg acgatgaaa 
      aagaggatg gtgacgaca gcccgataa gcagtccca 
      ctgggaaca actctaaag gtaaaagcg aatcaagtc 
      caggaaaat ctcccggaa gattggacg gagaagttg 
      aagtaaaaa cagatcctg attgtaaag acgaagaca 
      tcaaagttc ctttacatg ctcaagttt cagatccgg 
      atgaagctt atactctat tcatggcca ataataatt 
      ggtacatat tccttcgcc ttcatgctg ttttatgcg 
      aacgtttgg cgaaaatct acgaacgtg ctgtaattc 
      tggcagctg aagaagcaa aatctcgaa caggtcgta 
      aagaaagta cagccgttg cacttcgat tgaaaccaa 
      agcctcaga ttgaagttg aagattatt acgttgcat 
      ttttggata tggttaaaa atctcttgg atggtaata 
      tggacgcta atacttacg aagatactc ttcgagaaa 
      tgttcggta ttcatgctt atattgcgt tcactttgg 
      ataaagtgg tttcgtatg cggttcgtc aacttcagc 
      actgtgtga ctgaacgaa ctgcaattg cttgcactg 
      atttatttg tcaaggaac aaaaacgtg gagctaccg 
      gaggatcat gttctacag cgcacaaaa gagctcatc 
      tagagatgg cgtatcaaa ggctaggcg aaaaagctg 
      tacaagacg agaattgtt tcaagatct atatctata 
      agaaggatt gtaggatga ctattgagt tgcttgata 
      cggattcag aggatccga aaaaggttg aagatacga 
      aaaagtgga gttcgtata aagagtgtt acgcggata 
      taacacatg tggacaata aaccgcgtt cgccggtct 
      atttaaatc ggaatttaa ggttgtata aaaaacgta 
      tggggcttc ataataaaa cgtcatcgg tggtggaaa 
      aaaagaata aacaacaaa gggaagaga acgacgaac 
      aaaagaaga atcaagatg aagagaaca aaaagacaa 
      atagggcaa cgaccgacg aagcggaca ataaatttg 
      caatgttgg aggagttgg agaggaagt ttagcgaat 
      gtcctttgc ctgaaagaa atcaaacgg aacatccgg 
      gatatgaag tttcggatg aaacgcaat gtaaattta 
      attcacagg atccgaaaa ttatgttta cggttaaca 
      aggatagtt acttgtata aaataagtg ccttttcga 
      gagcgagaa agagtcatc ctgcgataa cgaaacgct 
      tgtcatgta aatttcgcg cgtggctga aacattggg 
      caagtgaga acgttagtg acgtgcaac ataaacagt 
      gtaccgatt ggttgatgg gtcgcggcg aaaatgtcg 
      tacctaacc gaacacggg tgctaacag acaatgatc 
      tttcacgaa ctccttatg taccataca acagataca 
      gagtcgaaa ggctgaacg aagatgtta ctgctacgt 
      gatgttgac gatgaatca atcaataaa ttagcattc 
      atatatttc tcttcctga aattgttgt ttttttttt 
      aattttttg tgcctgtag tgtttttct ttgtttaag 
      tcctcttga gttgaattt cgtggcgcg ttgttttct 
      tttttaagt ttaatgttg tgtttgcgc cccatcgtt 
      tttatgttg tgaaattat ttattgatt tatttataa 
      cgttaaaca tgccctcga agtgatact tttttatgt 
      aagttaaga actaaacgt acttgtttt ttatgcaaa 
      attttcccc cctacaata aaagattat aggtatgtg 
      gtgttttag tttaaaaat ttatcagta gtgccatag 
      tcgttttta aactaaaac attttagcc tgttgcagg 
      tcttttatt atagtacaa aaaataggt tatgttttt 
      gtattgctc ttatacgtt ttttttttt tttttcata 
      aattattga tttcctgtt atggttctt tgttaaccg 
      aatcatttt ttaacacta atgttgaca tatccttgg 
      tgaagtttt gttgtgtaa ttgaattac gactcgcct 
      gattttggt tggcaataa agtgcttcc aaagatttg 
      atatgggca gtcgaaaac ttagcaact aagtcataa 
      aaatatata tttttgtac acagagata tgtcagcat 
      gatccgcat cgccatcca gtttgtata tattaaaaa 
      tgaaaagaa aaataacca aacattttt caatagtat 
      aaaacattc cattttata cgatcactt cttcacttg 
      tccatgtat ttattaaag tttggttat ttttccaac 
      ttctcactt ttaattatt gtaaatttt aagtgtata 
      ggattaaaa gtgggagta atatgtaca caattaaag 
      attttaaaa actaaaatg ctacactgt acataccat 
      ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY