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Gene information
General information
TC009300 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1764066..1765501
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009300
      iBeetle screen iB_04615

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      Fertility
      egg production: number no/almost no eggs -
      tropharium shape irregular - Number of animals showing the phenotype: 4

      tropharium size decreased - Number of animals showing the phenotype: 4
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009300 RA
        MSQTLRCAL RSLCTPLKR DVLKLAPPL SSVLNTRFL 
        YAKATLGLD GFAEQSKQI HQQMENIAG KFREKMTTY 
        SSEDSQNMI FTEDLKNMV YMAESDADV ELVVKMTKK 
        FNSQNKELR FGNFVFGPV IMRMFYTHN KADLALECF 
        KAPEFDGIF DQLITYQIL LDLLYENGK YQEILDTFE 
        VIKGRQLEG TKYPRNSVV LVMAACYKM NSKESVEYA 
        LKFWNELRG AGHFPMRRA TTFCAGLAL NQGMPEAAL 
        EIISNVRNP NYVTIRNIK ARALSEIGR LDDVITILK 
        SVISDDRPG EVAHTFNRD VLEAIKAKV LEADNPEIS 
        AEFNRLEKL FEKEGHITN ETLDQHLCS EIQKPPVMN 
        NRQGNRFNR FQQSDRRPS PGFKRPSYR PGLKDLI
      CDS sequence
      • >TC009300 RA
        atgtctcaa actttacgg tgcgctttg cgcagcttg 
        tgcaccccc ttgaagcgc gacgtttta aagttggca 
        cccccattg tcgtctgtt ttgaacacg aggtttttg 
        tatgcgaaa gcaacgctg ggtctcgat gggtttgcc 
        gaacagagc aaacaaatc caccaacag atggaaaat 
        atagctggg aagtttcgc gagaaaatg accacgtac 
        tcgtcggaa gactcccaa aacatgatt tttacggag 
        gacttgaag aatatggtt tatatggct gagagtgac 
        gccgatgtt gagttggtg gtcaaaatg acgaaaaag 
        tttaattcg caaaataag gagttgagg tttgggaat 
        tttgtcttt ggaccggtc ataatgcgc atgttttat 
        actcataat aaggccgat ttggcgctt gagtgtttc 
        aaggcgccg gagttcgac ggtattttt gaccagctg 
        atcacgtac caaatcctt ttggacttg ctgtatgaa 
        aatggaaag tatcaagag attttggac acgtttgaa 
        gtaatcaaa ggccgacag ttagaaggg actaaatat 
        ccccgaaat tcagtggtt ttggtaatg gcagcgtgt 
        tacaaaatg aactcgaag gagagtgtg gagtatgcc 
        ttaaaattc tggaacgag ttgcgggga gcggggcat 
        ttccccatg cgtcgggcg accacattt tgcgccggt 
        ttggccctc aatcagggc atgcccgaa gccgcctta 
        gaaataatc tcaaacgtt cgaaacccc aattatgtc 
        acaatccgg aatattaaa gcccgtgcc ttgagcgaa 
        atcggccga ttagacgac gttatcaca attttaaaa 
        tctgtaatt tctgacgat aggcctggc gaagttgca 
        cacacgttc aatcgggat gtgttagag gcgatcaaa 
        gccaaggtt ttggaggct gataatccc gaaatatcg 
        gcagagttc aaccggttg gaaaaactg ttcgagaag 
        gagggacac atcactaat gaaactctg gaccaacac 
        ttgtgttcc gaaatccag aaaccgcca gttatgaat 
        aaccggcaa ggaaatcgg tttaaccgt ttccagcag 
        tctgatcgg aggccgtcg ccagggttc aagagaccc 
        tcatacagg cccggcttg aaggattta atttag
      mRNA sequence
      • >TC009300 RA
        aaggcaaaa aatgtctca aactttacg gtgcgcttt 
        gcgcagctt gtgcacccc cttgaagcg cgacgtttt 
        aaagttggc acccccatt gtcgtctgt tttgaacac 
        gaggttttt gtatgcgaa agcaacgct gggtctcga 
        tgggtttgc cgaacagag caaacaaat ccaccaaca 
        gatggaaaa tatagctgg gaagtttcg cgagaaaat 
        gaccacgta ctcgtcgga agactccca aaacatgat 
        ttttacgga ggacttgaa gaatatggt ttatatggc 
        tgagagtga cgccgatgt tgagttggt ggtcaaaat 
        gacgaaaaa gtttaattc gcaaaataa ggagttgag 
        gtttgggaa ttttgtctt tggaccggt cataatgcg 
        catgtttta tactcataa taaggccga tttggcgct 
        tgagtgttt caaggcgcc ggagttcga cggtatttt 
        tgaccagct gatcacgta ccaaatcct tttggactt 
        gctgtatga aaatggaaa gtatcaaga gattttgga 
        cacgtttga agtaatcaa aggccgaca gttagaagg 
        gactaaata tccccgaaa ttcagtggt tttggtaat 
        ggcagcgtg ttacaaaat gaactcgaa ggagagtgt 
        ggagtatgc cttaaaatt ctggaacga gttgcgggg 
        agcggggca tttccccat gcgtcgggc gaccacatt 
        ttgcgccgg tttggccct caatcaggg catgcccga 
        agccgcctt agaaataat ctcaaacgt tcgaaaccc 
        caattatgt cacaatccg gaatattaa agcccgtgc 
        cttgagcga aatcggccg attagacga cgttatcac 
        aattttaaa atctgtaat ttctgacga taggcctgg 
        cgaagttgc acacacgtt caatcggga tgtgttaga 
        ggcgatcaa agccaaggt tttggaggc tgataatcc 
        cgaaatatc ggcagagtt caaccggtt ggaaaaact 
        gttcgagaa ggagggaca catcactaa tgaaactct 
        ggaccaaca cttgtgttc cgaaatcca gaaaccgcc 
        agttatgaa taaccggca aggaaatcg gtttaaccg 
        tttccagca gtctgatcg gaggccgtc gccagggtt 
        caagagacc ctcatacag gcccggctt gaaggattt 
        aatttagtt attttttgt acatcttta gttgaataa 
        aaaatctca ctagac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY