Status
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Gene information
General information
TC009275 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2007836..2049843
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009275
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04611

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection



    Adult morphology
    elytra surface irregular - Number of animals showing the phenotype: 9


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009275 RA
      MEGSPDCAN DFLEIRDGL HGYNQLIEK KFCGKEFPP 
      VIESSDRYL WIHFSSDEN IEYKGFRAV YEFLPRPTG 
      WLVPDIPIC HLKKLLMEE GNITKSDIP AEIVEFRNK 
      YKVATDCIW EVRVKDYQR IQLQFKEFD LDKPNECDK 
      NFVQVFSTN TDLPSEEKK FCGSIADTV FSKKNVMFV 
      RFFAVFNST EESHFEANF TAYRDIDKQ SGDKKQDKC 
      TSEEFSCED ATCISKELR CNGRYNCRF RWDEDNCPG 
      AQTLTLNDD HIIVIMVIF SLILAGMCF TFVFNCVKK 
      LIRDHQTIQ EYIRQSREQ QLNELDKQE PTEKKLSKS 
      VSRSRSNSS PSMNSGHFD VANAATTPC YVPGGELLP 
      ILIRNERSM SPPNGDAYH TNIYTVDNE PLPEMCDSA 
      CQTRESLFT TQGYSSGNS TPNHSVHTN SPPAPFSTF 
      GYKKDAKFK AEAKIEVAA KKSSKFEDK RRPYSVQTT 
      KSAPDVIVT H
    • >TC009275 RB
      MEGSPDCAN DFLEIRDGL HGYNQLIEK KFCGKEFPP 
      VIESSDRYL WIHFSSDEN IEYKGFRAV YEFLPRPTG 
      WLVPDIPIC HLKKLLMEE GNITKSDIP AEIVEFRNK 
      YKVATDCIW EVRVKDYQR IQLQFKEFD LDKPNECDK 
      NFVQVFSTN TDLPSEEKK FCGSIADTV FSKKNVMFV 
      RFFAVFNST EESHFEANF TAYRDIDKQ SGDKKQDKC 
      TSEEFSCED ATCISKELR CNGRYNCRF RWDEDNCPG 
      AQTLTLNDD HIIVIMVIF SLILAGMCF TFVFNCVKK 
      LIRDHQTIQ EYIRQSREQ QLNELDKQE PTEKKLSKS 
      VSRSRSNSS PSMNSGHFD VANAATTPC YVPGGELLP 
      ILIRNERSM SPPNGDAYH TNIYTAMAV IALNSLCCP 
      LSTASRPGD THGELTAKL QEMPQSDAS AEPDIPRTD 
      PCYRFILGD RDKHEFYSP GFEDKPNYT NNTNCVDNE 
      PLPEMCDSA CQTRESLFT TQGYSSGNS TPNHSVHTN 
      SPPAPFSTF GYKKDAKFK AEAKIEVAA KKSSKFEDK 
      RRPYSVQTT KSAPDVIVT H
    CDS sequence
    • >TC009275 RA
      atggaggga agtcccgac tgtgcgaac gacttcctg 
      gagatcagg gacggactc cacggctac aatcagctg 
      atcgagaaa aagttctgc gggaaggag tttccgccg 
      gttattgag tcgtccgat cgctacttg tggattcac 
      ttcagttcg gacgaaaat atcgagtac aagggcttt 
      cgggcggtt tacgaattc ctgccaagg cccacggga 
      tggcttgtg cccgacatc cccatctgc cacctgaag 
      aagcttcta atggaagag ggcaacata acgaagagc 
      gacattccg gccgagatc gtcgagttc cgcaacaaa 
      tacaaggtg gccaccgac tgcatctgg gaagttcgt 
      gtcaaggac taccaaagg attcagctc cagttcaag 
      gagttcgac ttggacaag ccgaacgaa tgcgacaag 
      aacttcgtg caggtgttt tcaaccaac accgatctg 
      cccagtgag gagaaaaag ttctgcggt tccatcgct 
      gacacggtt ttttccaag aagaacgtc atgtttgtg 
      cgcttcttt gccgtgttc aactcgact gaagagtcg 
      cattttgag gcgaatttc acggcttat agggacatc 
      gacaaacag agcggcgat aagaagcag gacaagtgc 
      actagcgag gagttcagc tgtgaggat gctacttgt 
      atctcgaag gagctgcgc tgtaacggc agatacaac 
      tgcaggttt cgctgggat gaggacaac tgtccaggc 
      gctcagacg ctgacgctg aacgacgac cacatcatc 
      gtcatcatg gttattttt tcgctaatt ttggcaggc 
      atgtgcttc acttttgtt ttcaactgt gtcaagaaa 
      ctcataagg gaccaccaa actattcag gagtacatc 
      cgccaaagc agggagcag cagctcaac gagttggac 
      aaacaagag ccgacggag aagaagctg tccaaatcg 
      gtttccagg tcgcgttcc aacagctcg ccctcgatg 
      aattcgggg cactttgac gtagccaac gccgccacc 
      accccctgc tacgttccc gggggcgaa ctgttgccc 
      atactcatc cgcaacgag cgcagcatg agtccgccc 
      aacggggat gcttaccac accaacata tacacggtg 
      gacaacgag ccgctgccg gagatgtgc gacagcgcg 
      tgccagacc cgcgagagc ctcttcaca acgcagggc 
      tacagctcc ggcaacagc acgcccaac cacagcgtc 
      cacaccaac tcgccccca gcccccttc agcaccttc 
      ggctacaag aaggacgcc aagttcaag gccgaggcc 
      aagatcgag gtggcggcg aagaagtcg agcaagttc 
      gaggacaaa cgccgcccc tacagcgtg caaacgacc 
      aagtctgcc cccgacgtc atcgtcacg cactga
    • >TC009275 RB
      atggaggga agtcccgac tgtgcgaac gacttcctg 
      gagatcagg gacggactc cacggctac aatcagctg 
      atcgagaaa aagttctgc gggaaggag tttccgccg 
      gttattgag tcgtccgat cgctacttg tggattcac 
      ttcagttcg gacgaaaat atcgagtac aagggcttt 
      cgggcggtt tacgaattc ctgccaagg cccacggga 
      tggcttgtg cccgacatc cccatctgc cacctgaag 
      aagcttcta atggaagag ggcaacata acgaagagc 
      gacattccg gccgagatc gtcgagttc cgcaacaaa 
      tacaaggtg gccaccgac tgcatctgg gaagttcgt 
      gtcaaggac taccaaagg attcagctc cagttcaag 
      gagttcgac ttggacaag ccgaacgaa tgcgacaag 
      aacttcgtg caggtgttt tcaaccaac accgatctg 
      cccagtgag gagaaaaag ttctgcggt tccatcgct 
      gacacggtt ttttccaag aagaacgtc atgtttgtg 
      cgcttcttt gccgtgttc aactcgact gaagagtcg 
      cattttgag gcgaatttc acggcttat agggacatc 
      gacaaacag agcggcgat aagaagcag gacaagtgc 
      actagcgag gagttcagc tgtgaggat gctacttgt 
      atctcgaag gagctgcgc tgtaacggc agatacaac 
      tgcaggttt cgctgggat gaggacaac tgtccaggc 
      gctcagacg ctgacgctg aacgacgac cacatcatc 
      gtcatcatg gttattttt tcgctaatt ttggcaggc 
      atgtgcttc acttttgtt ttcaactgt gtcaagaaa 
      ctcataagg gaccaccaa actattcag gagtacatc 
      cgccaaagc agggagcag cagctcaac gagttggac 
      aaacaagag ccgacggag aagaagctg tccaaatcg 
      gtttccagg tcgcgttcc aacagctcg ccctcgatg 
      aattcgggg cactttgac gtagccaac gccgccacc 
      accccctgc tacgttccc gggggcgaa ctgttgccc 
      atactcatc cgcaacgag cgcagcatg agtccgccc 
      aacggggat gcttaccac accaacata tacacggca 
      atggcggtt atagcgctc aactcactt tgctgccct 
      ttatccacg gcttccaga ccaggtgac acccacgga 
      gagctgact gcgaagtta caggagatg ccgcaatcg 
      gacgcttcc gccgaacca gatataccc aggacggat 
      ccctgttat cggtttatt ctgggggac agggacaaa 
      cacgagttt tatagtccg ggattcgag gataagccc 
      aactatacc aacaacacc aactgcgtg gacaacgag 
      ccgctgccg gagatgtgc gacagcgcg tgccagacc 
      cgcgagagc ctcttcaca acgcagggc tacagctcc 
      ggcaacagc acgcccaac cacagcgtc cacaccaac 
      tcgccccca gcccccttc agcaccttc ggctacaag 
      aaggacgcc aagttcaag gccgaggcc aagatcgag 
      gtggcggcg aagaagtcg agcaagttc gaggacaaa 
      cgccgcccc tacagcgtg caaacgacc aagtctgcc 
      cccgacgtc atcgtcacg cactga
    mRNA sequence
    • >TC009275 RA
      agcaagagt taagatgca aatttcctc ctgcggcac 
      actttatca ggctggact ttcgggact acttcgaaa 
      tggagggaa gtcccgact gtgcgaacg acttcctgg 
      agatcaggg acggactcc acggctaca atcagctga 
      tcgagaaaa agttctgcg ggaaggagt ttccgccgg 
      ttattgagt cgtccgatc gctacttgt ggattcact 
      tcagttcgg acgaaaata tcgagtaca agggctttc 
      gggcggttt acgaattcc tgccaaggc ccacgggat 
      ggcttgtgc ccgacatcc ccatctgcc acctgaaga 
      agcttctaa tggaagagg gcaacataa cgaagagcg 
      acattccgg ccgagatcg tcgagttcc gcaacaaat 
      acaaggtgg ccaccgact gcatctggg aagttcgtg 
      tcaaggact accaaagga ttcagctcc agttcaagg 
      agttcgact tggacaagc cgaacgaat gcgacaaga 
      acttcgtgc aggtgtttt caaccaaca ccgatctgc 
      ccagtgagg agaaaaagt tctgcggtt ccatcgctg 
      acacggttt tttccaaga agaacgtca tgtttgtgc 
      gcttctttg ccgtgttca actcgactg aagagtcgc 
      attttgagg cgaatttca cggcttata gggacatcg 
      acaaacaga gcggcgata agaagcagg acaagtgca 
      ctagcgagg agttcagct gtgaggatg ctacttgta 
      tctcgaagg agctgcgct gtaacggca gatacaact 
      gcaggtttc gctgggatg aggacaact gtccaggcg 
      ctcagacgc tgacgctga acgacgacc acatcatcg 
      tcatcatgg ttatttttt cgctaattt tggcaggca 
      tgtgcttca cttttgttt tcaactgtg tcaagaaac 
      tcataaggg accaccaaa ctattcagg agtacatcc 
      gccaaagca gggagcagc agctcaacg agttggaca 
      aacaagagc cgacggaga agaagctgt ccaaatcgg 
      tttccaggt cgcgttcca acagctcgc cctcgatga 
      attcggggc actttgacg tagccaacg ccgccacca 
      ccccctgct acgttcccg ggggcgaac tgttgccca 
      tactcatcc gcaacgagc gcagcatga gtccgccca 
      acggggatg cttaccaca ccaacatat acacggtgg 
      acaacgagc cgctgccgg agatgtgcg acagcgcgt 
      gccagaccc gcgagagcc tcttcacaa cgcagggct 
      acagctccg gcaacagca cgcccaacc acagcgtcc 
      acaccaact cgcccccag cccccttca gcaccttcg 
      gctacaaga aggacgcca agttcaagg ccgaggcca 
      agatcgagg tggcggcga agaagtcga gcaagttcg 
      aggacaaac gccgcccct acagcgtgc aaacgacca 
      agtctgccc ccgacgtca tcgtcacgc actgacagt 
      tgtccaccg ccgagtggg agccgtaca agcggcgcc 
      acccccggc atttcctga ggacgaccg aaaaacgcc 
      tccacagag accgaaacg tcgacacgc agacttacg 
      acgccgagt tcgggacgt gatatccgt gggtgcggg 
      gtttccccg agtgcgact tttctcgac gactcttgt 
      gatttcgtt cacgtggac cagctacgt ttttcactt 
      tcaacgcaa aatgtgtct gtgtcttcc acttccgtt 
      cctttcctc caactttaa attcgttta caggttttg 
      gaaaacact tgattttgt ccaaatctc gacaccgtt 
      tacttgcct ttcctgtta atgtttata actgagttt 
      tgagaaatt ttattgata gactaaata aattgatat 
      gtcttta
    • >TC009275 RB
      agcaagagt taagatgca aatttcctc ctgcggcac 
      actttatca ggctggact ttcgggact acttcgaaa 
      tggagggaa gtcccgact gtgcgaacg acttcctgg 
      agatcaggg acggactcc acggctaca atcagctga 
      tcgagaaaa agttctgcg ggaaggagt ttccgccgg 
      ttattgagt cgtccgatc gctacttgt ggattcact 
      tcagttcgg acgaaaata tcgagtaca agggctttc 
      gggcggttt acgaattcc tgccaaggc ccacgggat 
      ggcttgtgc ccgacatcc ccatctgcc acctgaaga 
      agcttctaa tggaagagg gcaacataa cgaagagcg 
      acattccgg ccgagatcg tcgagttcc gcaacaaat 
      acaaggtgg ccaccgact gcatctggg aagttcgtg 
      tcaaggact accaaagga ttcagctcc agttcaagg 
      agttcgact tggacaagc cgaacgaat gcgacaaga 
      acttcgtgc aggtgtttt caaccaaca ccgatctgc 
      ccagtgagg agaaaaagt tctgcggtt ccatcgctg 
      acacggttt tttccaaga agaacgtca tgtttgtgc 
      gcttctttg ccgtgttca actcgactg aagagtcgc 
      attttgagg cgaatttca cggcttata gggacatcg 
      acaaacaga gcggcgata agaagcagg acaagtgca 
      ctagcgagg agttcagct gtgaggatg ctacttgta 
      tctcgaagg agctgcgct gtaacggca gatacaact 
      gcaggtttc gctgggatg aggacaact gtccaggcg 
      ctcagacgc tgacgctga acgacgacc acatcatcg 
      tcatcatgg ttatttttt cgctaattt tggcaggca 
      tgtgcttca cttttgttt tcaactgtg tcaagaaac 
      tcataaggg accaccaaa ctattcagg agtacatcc 
      gccaaagca gggagcagc agctcaacg agttggaca 
      aacaagagc cgacggaga agaagctgt ccaaatcgg 
      tttccaggt cgcgttcca acagctcgc cctcgatga 
      attcggggc actttgacg tagccaacg ccgccacca 
      ccccctgct acgttcccg ggggcgaac tgttgccca 
      tactcatcc gcaacgagc gcagcatga gtccgccca 
      acggggatg cttaccaca ccaacatat acacggcaa 
      tggcggtta tagcgctca actcacttt gctgccctt 
      tatccacgg cttccagac caggtgaca cccacggag 
      agctgactg cgaagttac aggagatgc cgcaatcgg 
      acgcttccg ccgaaccag atataccca ggacggatc 
      cctgttatc ggtttattc tgggggaca gggacaaac 
      acgagtttt atagtccgg gattcgagg ataagccca 
      actatacca acaacacca actgcgtgg acaacgagc 
      cgctgccgg agatgtgcg acagcgcgt gccagaccc 
      gcgagagcc tcttcacaa cgcagggct acagctccg 
      gcaacagca cgcccaacc acagcgtcc acaccaact 
      cgcccccag cccccttca gcaccttcg gctacaaga 
      aggacgcca agttcaagg ccgaggcca agatcgagg 
      tggcggcga agaagtcga gcaagttcg aggacaaac 
      gccgcccct acagcgtgc aaacgacca agtctgccc 
      ccgacgtca tcgtcacgc actgacagt tgtccaccg 
      ccgagtggg agccgtaca agcggcgcc acccccggc 
      atttcctga ggacgaccg aaaaacgcc tccacagag 
      accgaaacg tcgacacgc agacttacg acgccgagt 
      tcgggacgt gatatccgt gggtgcggg gtttccccg 
      agtgcgact tttctcgac gactcttgt gatttcgtt 
      cacgtggac cagctacgt ttttcactt tcaacgcaa 
      aatgtgtct gtgtcttcc acttccgtt cctttcctc 
      caactttaa attcgttta caggttttg gaaaacact 
      tgattttgt ccaaatctc gacaccgtt tacttgcct 
      ttcctgtta atgtttata actgagttt tgagaaatt 
      ttattgata gactaaata aattgatat gtcttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY