Status
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Gene information
General information
TC009222 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2964361..2966658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009222
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04605

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009222 RA
      MRHVRLPGQ NIKKLTFRS QIGDILKEL NKTEGPQND 
      DSKPSCSFD VAQKRRYNI RARKKMSYI ENLSVKKTT 
      YKCKVCSFT ERTKKVMLK HLQTHVGAP LSCFKCKKT 
      FNGSVAYKW HLGHYCTPV FYDKWKYKC SLCPREFVS 
      KRTLASHLQ GHKRNNCAY CDKVLTRRV HLIRHLLEV 
      HSIKLERDT YKCDHCEKR YIKKCSLYY HLKQHLPDK 
      YICLECGKV SETVEENEE HKKQHDLQK HFKCAKCGD 
      RFSRRQQYL FHLKRHNQY KCVTCDEFY ASRTKVIKH 
      KQQGHEVEG LNPRFICPH CPVAFHRET RFHIHMRKH 
      SEDNRSINC KYCKKTFVS PYAYYKHCI TINHEKFSP 
      EINDIACEK CGKHFAKRY DLRQHLYRV HNTSKQFVS 
      CQYCNYKTV HKCNMDRHV ALHFRKSNQ YTCEYCGKN 
      FDSIANLND HITYNHIQT RQFKCDKCD KSFKRNSEL 
      ARHQDSHSD ERPHVCTLC GKTYKRLNH LKRHEKQAH 
      HIVKETRRI KNVSEEQKK SESNCENLP ELNYWIDSE 
      DLYRVYNDV SLTYGMCEG LEGADLAQN ENNSC
    CDS sequence
    • >TC009222 RA
      atgcgccac gtgcgcctc cctggccaa aacatcaaa 
      aaactaaca tttcgttcc cagattggt gatattttg 
      aaggaattg aacaagaca gaggggcca caaaatgac 
      gattcgaag ccgagctgt tcgttcgat gttgcacaa 
      aaacgtcga tacaatatc agggctcgt aaaaaaatg 
      tcatacatt gagaatctc tccgttaaa aaaactacc 
      tacaagtgt aaagtgtgc agttttact gaaaggacg 
      aaaaaagtg atgttgaaa catctgcag acacacgtc 
      ggggccccc ttgtcttgt ttcaagtgc aaaaaaacg 
      ttcaatggc agtgtggcg tacaagtgg catttgggg 
      cactactgc acccccgtg ttttacgac aagtggaaa 
      tataagtgt agtttatgc cctcgagaa tttgtttca 
      aaaagaaca ttagccagt catttacaa ggccacaaa 
      agaaataat tgtgcctac tgtgataaa gtgttaact 
      cgaagagtg cacttaatc cgacacttg ttagaagtt 
      cactcgatt aaactagaa agagatacg tacaagtgt 
      gatcattgt gaaaaaagg tatataaaa aagtgttca 
      ttatattat cacttgaaa caacacctt ccagacaaa 
      tatatatgt ctggagtgt ggcaaagtt agcgaaaca 
      gttgaagaa aatgaagaa cacaaaaaa caacacgat 
      ttacaaaaa cacttcaag tgtgcaaaa tgtggcgat 
      cgcttctcc cgacgtcaa caatattta tttcattta 
      aaaagacat aatcaatac aaatgcgtg acttgtgac 
      gaattttat gcctcaaga accaaagta atcaagcac 
      aagcaacaa gggcatgaa gttgaaggg ctaaacccc 
      cgttttata tgccctcac tgtccggtg gcatttcac 
      cgagaaacc cgctttcac atccacatg cgtaaacac 
      tcagaggat aatcgcagt atcaactgc aaatattgc 
      aagaaaact tttgtttcg ccctacgcc tactacaag 
      cattgtatt acgataaat cacgagaag ttcagtccg 
      gaaattaac gacatagcg tgcgaaaag tgtggcaaa 
      catttcgcc aaacgctac gatttgaga caacacttg 
      tatcgagtg cataatacg tcgaaacaa ttcgtttcg 
      tgtcaatat tgcaactac aaaactgtt cataagtgt 
      aatatggac cgacacgtg gcgttacat tttcgtaaa 
      agcaatcaa tacacgtgc gaatattgt gggaaaaat 
      ttcgacagt atcgccaac ttgaacgac cacattacc 
      tacaatcac atacagact aggcagttc aagtgcgat 
      aagtgtgat aagtcgttt aaaaggaat tccgaattg 
      gcgcggcac caagacagc cattcggat gaaaggcca 
      catgtttgc acgttgtgt ggcaagacg tataagagg 
      ctaaatcat ttaaaaagg catgaaaag caggcacat 
      catattgtt aaagaaacc agaaggatt aaaaatgtg 
      tcagaagag caaaaaaaa tccgaatcg aattgtgaa 
      aatttgccc gaattgaat tattggatc gatagtgag 
      gacctatac agggtgtat aatgatgtg tcgttaact 
      tatggcatg tgtgaaggg ttggagggc gcagacttg 
      gcgcaaaat gaaaacaac tcgtgttaa
    mRNA sequence
    • >TC009222 RA
      tcatttttg acgtctcgt ctacacttt ttttatcat 
      cattattat ttaatcaaa ttatttaga attttttgt 
      acgaagtga gtaaacaat gcgccacgt gcgcctccc 
      tggccaaaa catcaaaaa actaacatt tcgttccca 
      gattggtga tattttgaa ggaattgaa caagacaga 
      ggggccaca aaatgacga ttcgaagcc gagctgttc 
      gttcgatgt tgcacaaaa acgtcgata caatatcag 
      ggctcgtaa aaaaatgtc atacattga gaatctctc 
      cgttaaaaa aactaccta caagtgtaa agtgtgcag 
      ttttactga aaggacgaa aaaagtgat gttgaaaca 
      tctgcagac acacgtcgg ggccccctt gtcttgttt 
      caagtgcaa aaaaacgtt caatggcag tgtggcgta 
      caagtggca tttggggca ctactgcac ccccgtgtt 
      ttacgacaa gtggaaata taagtgtag tttatgccc 
      tcgagaatt tgtttcaaa aagaacatt agccagtca 
      tttacaagg ccacaaaag aaataattg tgcctactg 
      tgataaagt gttaactcg aagagtgca cttaatccg 
      acacttgtt agaagttca ctcgattaa actagaaag 
      agatacgta caagtgtga tcattgtga aaaaaggta 
      tataaaaaa gtgttcatt atattatca cttgaaaca 
      acaccttcc agacaaata tatatgtct ggagtgtgg 
      caaagttag cgaaacagt tgaagaaaa tgaagaaca 
      caaaaaaca acacgattt acaaaaaca cttcaagtg 
      tgcaaaatg tggcgatcg cttctcccg acgtcaaca 
      atatttatt tcatttaaa aagacataa tcaatacaa 
      atgcgtgac ttgtgacga attttatgc ctcaagaac 
      caaagtaat caagcacaa gcaacaagg gcatgaagt 
      tgaagggct aaacccccg ttttatatg ccctcactg 
      tccggtggc atttcaccg agaaacccg ctttcacat 
      ccacatgcg taaacactc agaggataa tcgcagtat 
      caactgcaa atattgcaa gaaaacttt tgtttcgcc 
      ctacgccta ctacaagca ttgtattac gataaatca 
      cgagaagtt cagtccgga aattaacga catagcgtg 
      cgaaaagtg tggcaaaca tttcgccaa acgctacga 
      tttgagaca acacttgta tcgagtgca taatacgtc 
      gaaacaatt cgtttcgtg tcaatattg caactacaa 
      aactgttca taagtgtaa tatggaccg acacgtggc 
      gttacattt tcgtaaaag caatcaata cacgtgcga 
      atattgtgg gaaaaattt cgacagtat cgccaactt 
      gaacgacca cattaccta caatcacat acagactag 
      gcagttcaa gtgcgataa gtgtgataa gtcgtttaa 
      aaggaattc cgaattggc gcggcacca agacagcca 
      ttcggatga aaggccaca tgtttgcac gttgtgtgg 
      caagacgta taagaggct aaatcattt aaaaaggca 
      tgaaaagca ggcacatca tattgttaa agaaaccag 
      aaggattaa aaatgtgtc agaagagca aaaaaaatc 
      cgaatcgaa ttgtgaaaa tttgcccga attgaatta 
      ttggatcga tagtgagga cctatacag ggtgtataa 
      tgatgtgtc gttaactta tggcatgtg tgaagggtt 
      ggagggcgc agacttggc gcaaaatga aaacaactc 
      gtgttaaaa atcaaagta tttattatt tgtacaagg 
      tggctcaga aataaggac ataatcaac cgtttattt 
      cgtagcatt ggaactaac tagattatt tctgagaca 
      tcttaatta atatttatt tttaagtaa tgttactaa 
      aaaatgaat aaataaaga tttgaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY