Status
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Gene information
General information
TC031185
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009194,TC009193
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3251970..3257954
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031185
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04604

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized -

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 4

    Adult morphology
    elytra shortened - Number of animals showing the phenotype: 4


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031185 RA
      MSTTAFVDR IDPFAKRSL KKKTKKSQG SSRYRNSQD 
      VELQPLPLL KDVPASEQE ELFIRKLRQ CCVPFDFMD 
      PVADLKGKE VKRCTLNEL VDYITGGRG VLTEPVYPE 
      IIKMISANL FRTLPPSEN PDFDPEEDD PTLEASWPH 
      LQLVYEFFL RFLESSDFQ PAIGKRVID QKFVLQLLE 
      LFDSEDPRE RDFLKTVLH RIYGKFLGL RAFIRKQIN 
      NIFLRFVYE TEHFNGVGE LLEILGSII NGFALPLKA 
      EHKQFLVKV LIPLHKVKC LSLYHAQLA YCVVQFLEK 
      DPTLTEPVV RGLLKYWPK TCSQKEVMF LGEIEEVLD 
      VIEPSQFTR IQEPLFRQI AKCVSSPHF QVAERALYF 
      WNNEYIMSL MEENNHVIM PIMFPALYR ISKEHWNQT 
      IVALVYNVL KTFMEMNSK LFDELTVSY KAERQKEKK 
      REKERDELW KKLAELELN HNKNLPAQN NSPPSKK
    CDS sequence
    • >TC031185 RA
      atgtcgacc acggcgttc gtcgatcgg atcgatccc 
      ttcgccaag cgctcgctc aagaagaag acgaaaaag 
      tcgcaaggc agctccagg tacaggaac tcgcaggac 
      gtggagctg cagccgctg ccgctgctc aaagacgtc 
      ccggcatcc gaacaggag gagttgttt atacgcaaa 
      cttagacaa tgttgtgtg ccattcgat tttatggat 
      ccggtggcg gatctcaaa ggaaaagaa gtgaaacgt 
      tgtactttg aatgaacta gtggattat ataacgggc 
      ggcagggga gttttgacc gaacccgtc tatccagaa 
      attattaaa atgatttcg gcgaatctt tttcgaaca 
      ctgcctccc agcgaaaat cccgatttc gatccggaa 
      gaggacgac cccacgctg gaagcaagt tggccgcat 
      ctccaacta gtttacgaa ttctttttg cgatttttg 
      gaaagttcg gattttcag ccggccata ggaaaacgt 
      gtgattgat cagaagttt gttctacaa ttattagaa 
      ttattcgat tcggaagat ccgagagaa agggatttt 
      ctaaagacg gtgctgcac cgcatttat ggaaagttc 
      ctcgggcta agggccttt attcgcaaa caaataaac 
      aacatattc cttaggttc gtgtatgag actgaacat 
      tttaacggc gtcggtgaa ctgttggag attttgggt 
      tcgatcata aacggcttt gcgttacca ttgaaagcc 
      gaacacaaa cagttcctg gtgaaggtg ctcattcct 
      ttgcataag gtcaagtgc ctgtcgttg tatcacgcg 
      cagctcgcc tattgcgtg gttcaattt ttggagaag 
      gatccgacg ttgacggaa ccagtggtt cgaggtttg 
      ctcaaatac tggccgaaa acgtgtagt caaaaagag 
      gtgatgttt cttggtgaa atcgaagaa gtattggat 
      gttattgaa ccgtcacaa tttacacga atccaggaa 
      ccgttgttt agacaaatc gccaaatgc gtgtctagt 
      ccacatttt caagtggcc gagcgtgct ttgtatttc 
      tggaacaac gagtacatc atgtcgctg atggaagaa 
      aacaatcac gtaataatg ccgattatg tttcccgca 
      ttgtatcga atttcgaaa gagcattgg aaccaaact 
      atagtcgca cttgtttat aatgtgctc aagactttt 
      atggaaatg aattcgaag ttgtttgat gaactcacg 
      gttagttat aaagcggaa aggcaaaaa gagaaaaaa 
      cgtgagaag gaacgagac gaattatgg aaaaagtta 
      gccgaactc gaacttaat cataataaa aatttaccg 
      gcacaaaat aatagtcct cccagcaaa aagtga
    mRNA sequence
    • >TC031185 RA
      ttggttgtg aaaaccagt tgtgttttg gtgcgcgca 
      gtacccccc atctatttt cagccctag gtacttacg 
      gcatcggtt cgtgcaaaa atcgcgtaa aaacccgtc 
      aatagcgct taaaccccc ggtgtttgg tggcccctt 
      tgtgcaaaa cctagtctt ttgggcggc gttcgcgat 
      gttggtgtg ttcgcaccg cgcatggat cggctgaac 
      gcgagtatt ttgtgtcaa agcaaccag ctgatggcg 
      ggtgaaaat actggactc gcttggcag ttgttcgat 
      tttccgcgt ctttccgcc gattcctgc taactgttt 
      gtggttgtg gtgcgcgag gcggcccca gatcggcga 
      ctcggcttg cgttccctc gaccgggct ttgggcgtg 
      caccttgtc attggagga tgcgttcga agggggctg 
      gtgccgttt gacgtagac gtccaggtg aaatgtcgc 
      gcgcgtaaa ccgagccag atcgagtag ccagcatgt 
      cgaccacgg cgttcgtcg atcggatcg atcccttcg 
      ccaagcgct cgctcaaga agaagacga aaaagtcgc 
      aaggcagct ccaggtaca ggaactcgc aggacgtgg 
      agctgcagc cgctgccgc tgctcaaag acgtcccgg 
      catccgaac aggaggagt tgtttatac gcaaactta 
      gacaatgtt gtgtgccat tcgatttta tggatccgg 
      tggcggatc tcaaaggaa aagaagtga aacgttgta 
      ctttgaatg aactagtgg attatataa cgggcggca 
      ggggagttt tgaccgaac ccgtctatc cagaaatta 
      ttaaaatga tttcggcga atctttttc gaacactgc 
      ctcccagcg aaaatcccg atttcgatc cggaagagg 
      acgacccca cgctggaag caagttggc cgcatctcc 
      aactagttt acgaattct ttttgcgat ttttggaaa 
      gttcggatt ttcagccgg ccataggaa aacgtgtga 
      ttgatcaga agtttgttc tacaattat tagaattat 
      tcgattcgg aagatccga gagaaaggg attttctaa 
      agacggtgc tgcaccgca tttatggaa agttcctcg 
      ggctaaggg cctttattc gcaaacaaa taaacaaca 
      tattcctta ggttcgtgt atgagactg aacatttta 
      acggcgtcg gtgaactgt tggagattt tgggttcga 
      tcataaacg gctttgcgt taccattga aagccgaac 
      acaaacagt tcctggtga aggtgctca ttcctttgc 
      ataaggtca agtgcctgt cgttgtatc acgcgcagc 
      tcgcctatt gcgtggttc aatttttgg agaaggatc 
      cgacgttga cggaaccag tggttcgag gtttgctca 
      aatactggc cgaaaacgt gtagtcaaa aagaggtga 
      tgtttcttg gtgaaatcg aagaagtat tggatgtta 
      ttgaaccgt cacaattta cacgaatcc aggaaccgt 
      tgtttagac aaatcgcca aatgcgtgt ctagtccac 
      attttcaag tggccgagc gtgctttgt atttctgga 
      acaacgagt acatcatgt cgctgatgg aagaaaaca 
      atcacgtaa taatgccga ttatgtttc ccgcattgt 
      atcgaattt cgaaagagc attggaacc aaactatag 
      tcgcacttg tttataatg tgctcaaga cttttatgg 
      aaatgaatt cgaagttgt ttgatgaac tcacggtta 
      gttataaag cggaaaggc aaaaagaga aaaaacgtg 
      agaaggaac gagacgaat tatggaaaa agttagccg 
      aactcgaac ttaatcata ataaaaatt taccggcac 
      aaaataata gtcctccca gcaaaaagt gatacaaca 
      gcctaaaat tccccgccc attcatagc atggtaagt 
      gcagacaat ggagcatgc ttccaacac c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY