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Gene information
General information
TC009185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3294703..3296804
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009185
    iBeetle screen iB_04603

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (41 dpi)
    Days post injection


    Adult morphology
    hindwing folding not present - Number of animals showing the phenotype: 10

    Comment: adults open and close elytra the hole time (trying to fold hindwings proper)

    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009185 RA
      MNRLGNIRL PPLSAAVNV AGQTFRQMI PERAPGHER 
      VHFAQVNHT THGGTTDEH HEMTNIKQT TNPFLQENF 
      EAEDSFNED LKTRMSSVD FSEGSDFVE GKSDVKISE 
      YQAAWNVTN AIQGMFIVS LPFAVLRGG YWAIVAMVG 
      IAYICCYTG KILVECLYE FDVQTGRQV RVRDSYVSI 
      AKACFGKKY GARIVNIAQ IIELLMTCI LYVVVCGDL 
      MIGTFPDGA IDTRSWMML VGIFLLPLA FLKTLQSVS 
      LLSFWCTMS HILINAIIL GYCLLYIGD WGWGKVKWS 
      LDLENFPIS LGVIVFSYT SQIFLPTLE GNMEDPSKF 
      QWMLDWSHV WAAVFKALF GYLCFLTFQ NDTQQVITN 
      NLPSAGFKG LVNICLVIK ALLSYPLPY YAACELLER 
      AFFRGKPKT PFPTIWALD GELKVWGLA CRVGVVVFT 
      ILMACFIPH FAILMGFIG SFTGTMLSF IWPCYFHLK 
      LKREGMDSR TVAFDYFII SLGMLFGFV GIYDSGRAL 
      INAFQIGLP F
    CDS sequence
    • >TC009185 RA
      atgaaccgt ctgggcaac atccgcttg ccgcctctc 
      tccgccgcc gtcaacgtg gccggacag accttccgc 
      cagatgatc cccgagcgg gcccccgga cacgagcgc 
      gtccacttt gcacaagtc aaccacacc acacatggg 
      ggcaccacc gacgagcac cacgagatg accaacatt 
      aagcagacc accaacccc ttcctccag gagaacttc 
      gaggccgag gacagcttc aacgaggac ctcaagacc 
      cgcatgtcc tcggtcgac ttctccgaa ggctccgac 
      tttgtcgag gggaagtct gatgtgaag atcagcgag 
      tatcaggcc gcctggaac gtcaccaac gccatacag 
      ggcatgttt atcgtgtcg ctgccattt gcggtcctc 
      cgagggggc tactgggcc atcgttgcc atggtgggc 
      atcgcctac atttgctgt tacactggc aaaatcctg 
      gtggaatgt ctctacgaa tttgacgtc caaacagga 
      cgccaagtc cgagtccga gactcttac gtttcaatc 
      gctaaagct tgctttggc aaaaaatac ggcgctcgg 
      attgtaaat atagcccaa attatcgaa ctcctgatg 
      acttgcatc ttgtacgtg gtggtctgt ggcgacctg 
      atgatcggc accttccca gatggggct atagacacc 
      cgatcgtgg atgatgcta gtcgggatt ttcctcctc 
      cctttagcg ttcctcaag accctccag agcgtgagt 
      ctgctctcg ttctggtgc acgatgtcc cacatcctc 
      atcaacgcc atcatcctc ggctactgc ctcctctac 
      atcggcgac tggggctgg ggcaaagtc aagtggtcg 
      ctcgacctg gagaacttc cccataagc ctcggcgtg 
      atcgtcttc tcctacacc tcccagatc ttcctcccg 
      acccttgaa ggcaacatg gaggacccc tccaagttc 
      cagtggatg ctggattgg tcccacgtg tgggccgcc 
      gtcttcaaa gccctcttc ggctacctc tgcttcctc 
      actttccag aacgacacc caacaggtc atcaccaac 
      aacctcccc tcggccggt ttcaagggc ctggtgaac 
      atctgcctg gtcatcaag gccctcctc agctaccct 
      ttgccctac tacgccgcc tgcgagctg ctcgagcgc 
      gcctttttc cggggaaaa cccaaaacg cccttcccc 
      accatctgg gcccttgat ggcgagctg aaggtatgg 
      gggctggcg tgcagggtg ggggttgtg gtttttacc 
      atcctcatg gcctgcttt atcccgcat tttgccatt 
      ttgatgggg tttattggg agttttacg gggacgatg 
      cttagtttc atttggccg tgttatttc catttgaaa 
      ttgaaaagg gaagggatg gattcaagg acggtggct 
      tttgattat tttatcatt tccctcggt atgcttttc 
      gggtttgtc gggatttac gactcgggg agggcccta 
      attaacgcc ttccagatc gggcttccc ttttaa
    mRNA sequence
    • >TC009185 RA
      atgtaccac atctcctcc ggtcaaccg ccgccaaga 
      tggaaatca ccaccttcc aaatcgccc ccaaaatgg 
      tctaacctg cacttttcg cacacttaa agtaaaaaa 
      ttagcgttt gttccatct tggcggtgc tcagtgcca 
      tcacacaat gaaccgtct gggcaacat ccgcttgcc 
      gcctctctc cgccgccgt caacgtggc cggacagac 
      cttccgcca gatgatccc cgagcgggc ccccggaca 
      cgagcgcgt ccactttgc acaagtcaa ccacaccac 
      acatggggg caccaccga cgagcacca cgagatgac 
      caacattaa gcagaccac caacccctt cctccagga 
      gaacttcga ggccgagga cagcttcaa cgaggacct 
      caagacccg catgtcctc ggtcgactt ctccgaagg 
      ctccgactt tgtcgaggg gaagtctga tgtgaagat 
      cagcgagta tcaggccgc ctggaacgt caccaacgc 
      catacaggg catgtttat cgtgtcgct gccatttgc 
      ggtcctccg agggggcta ctgggccat cgttgccat 
      ggtgggcat cgcctacat ttgctgtta cactggcaa 
      aatcctggt ggaatgtct ctacgaatt tgacgtcca 
      aacaggacg ccaagtccg agtccgaga ctcttacgt 
      ttcaatcgc taaagcttg ctttggcaa aaaatacgg 
      cgctcggat tgtaaatat agcccaaat tatcgaact 
      cctgatgac ttgcatctt gtacgtggt ggtctgtgg 
      cgacctgat gatcggcac cttcccaga tggggctat 
      agacacccg atcgtggat gatgctagt cgggatttt 
      cctcctccc tttagcgtt cctcaagac cctccagag 
      cgtgagtct gctctcgtt ctggtgcac gatgtccca 
      catcctcat caacgccat catcctcgg ctactgcct 
      cctctacat cggcgactg gggctgggg caaagtcaa 
      gtggtcgct cgacctgga gaacttccc cataagcct 
      cggcgtgat cgtcttctc ctacacctc ccagatctt 
      cctcccgac ccttgaagg caacatgga ggacccctc 
      caagttcca gtggatgct ggattggtc ccacgtgtg 
      ggccgccgt cttcaaagc cctcttcgg ctacctctg 
      cttcctcac tttccagaa cgacaccca acaggtcat 
      caccaacaa cctcccctc ggccggttt caagggcct 
      ggtgaacat ctgcctggt catcaaggc cctcctcag 
      ctacccttt gccctacta cgccgcctg cgagctgct 
      cgagcgcgc ctttttccg gggaaaacc caaaacgcc 
      cttccccac catctgggc ccttgatgg cgagctgaa 
      ggtatgggg gctggcgtg cagggtggg ggttgtggt 
      ttttaccat cctcatggc ctgctttat cccgcattt 
      tgccatttt gatggggtt tattgggag ttttacggg 
      gacgatgct tagtttcat ttggccgtg ttatttcca 
      tttgaaatt gaaaaggga agggatgga ttcaaggac 
      ggtggcttt tgattattt tatcatttc cctcggtat 
      gcttttcgg gtttgtcgg gatttacga ctcggggag 
      ggccctaat taacgcctt ccagatcgg gcttccctt 
      ttaaaaacc caccttcag taaatacgg acatggtta 
      ccaactgct attgtcaat tcgtacgct tttagtcta 
      gagaggcgc aataaatga ttcaattat tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY