Status
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Gene information
General information
TC009184 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3300076..3323911
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009184
    iBeetle screen iB_04602

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009184 RA
      MNHSNETEL TVEDVLRSF REDNFVVIG LYVPVFVVA 
      LLANVLVIW VVIKDQYMR SVTNYFLVN LSVADLLVA 
      LVCMPNAAY AAYTSTYSF GEYTCKISA YFQCVSVAS 
      SIFTITAMS IDRYLAITK PFGFFNRCF NKRNTVIVI 
      LVLWLLSFV LFLPILLVT GTWKMDDIH LFNKSLDIP 
      AYFCREDWT TYFGSSGSE MRQALGITW FIFMFVVPG 
      MIMLFAYSM MSRTLCSGV PPFDSNDGL SCMQQRNRL 
      MRSRKRVAC ILLLLAFVF AGCWMPYHL VSLLDDLNN 
      GPYKTLKMN LVLLGHANS ALNPIIYCA LSRKFRRSI 
      KDLICIDIH FTRRRQNIQ WGDSSGSGT QLHYLHRIK 
      SVPTVHNQL HFTLGRLQS SQKTTKTCV V
    • >TC009184 RB
      MNHSNETEL TVEDVLRSF REDNFVVIG LYVPVFVVA 
      LLANVLVIW VVIKDQYMR RFLWCYNSI SRREAVTNY 
      FLVNLSVAD LLVALVCMP NAAYAAYTS TYSFGEYTC 
      KISAYFQCV SVASSIFTI TAMSIDRYL AITKPFGFF 
      NRCFNKRNT VIVILVLWL LSFVLFLPI LLVTGTWKM 
      DDIHLFNKS LDIPAYFCR EDWTTYFGS SGSEMRQAL 
      GITWFIFMF VVPGMIMLF AYSMMSRTL CSGVPPFDS 
      NDGLSCMQQ RNRLMRSRK RVACILLLL AFVFAGCWM 
      PYHLVSLLD DLNNGPYKT LKMNLVLLG HANSALNPI 
      IYCALSRKF RRSIKDLIC IDIHFTRRR QNIQWGDSS 
      GSGTQLHYL HRIKSVPTV HNQLHFTLG RLQSSQKTT 
      KTCVV
    CDS sequence
    • >TC009184 RA
      atgaatcac tcaaatgag accgaactg accgtcgaa 
      gatgtgctg cgctcgttc cgggaagat aattttgtt 
      gtgattgga ctttatgtg ccggtgttt gttgtcgcg 
      ctgttggcc aatgtgctc gttatttgg gtcgtcatc 
      aaggaccag tatatgaga agcgtcacc aactacttc 
      ttagtgaac ttatctgtg gccgacctc ttagtggca 
      ctcgtctgt atgcccaat gcggcttac gccgcctac 
      acttccacc tacagcttc ggagagtac acctgcaaa 
      atatcggcg tatttccaa tgcgtctca gtggcgtcg 
      agcatcttc accatcacc gccatgtcc atcgaccgg 
      tacctggcc atcaccaag cccttcggc ttcttcaac 
      cgctgcttc aacaagcgc aacaccgtc atcgtcatc 
      ctcgtcctg tggctcttg tccttcgtc ctcttcctg 
      cccatcctg ctggtgacc ggcacgtgg aaaatggac 
      gacatccac ctcttcaac aagagcctg gacatcccg 
      gcctacttc tgccgcgag gactggacc acctacttc 
      ggctcctcg ggctcggag atgcgccaa gcgctcggc 
      atcacctgg ttcatcttc atgttcgtg gtgccaggg 
      atgataatg ctgttcgcc tactcgatg atgagcagg 
      acgctgtgc tcgggggtt ccgcccttt gacagcaac 
      gatgggctg tcctgcatg caacagaga aacagacta 
      atgaggagc cgcaaacgc gtggcctgc atcctcctg 
      ctgctggcg ttcgtgttc gccggctgc tggatgccc 
      taccacctg gtgagcctc ctcgacgac ctgaacaac 
      ggcccctac aagaccctc aaaatgaac ctagtgctc 
      ctcggccac gccaattcc gccctcaac cccatcatc 
      tactgcgcc ctttcgcgc aaattccgg cgctccatc 
      aaagatctg atttgcatc gatatacat ttcaccaga 
      cgccggcaa aatattcag tggggcgac agttccggc 
      tcgggcaca caactccat tacttacac aggattaaa 
      tcagtgcca actgtgcat aatcagctt catttcacg 
      ctcggacgc ctgcagtca agccagaaa acgaccaag 
      acgtgcgtg gtttga
    • >TC009184 RB
      atgaatcac tcaaatgag accgaactg accgtcgaa 
      gatgtgctg cgctcgttc cgggaagat aattttgtt 
      gtgattgga ctttatgtg ccggtgttt gttgtcgcg 
      ctgttggcc aatgtgctc gttatttgg gtcgtcatc 
      aaggaccag tatatgaga aggtttctg tggtgttac 
      aacagcatt tcgcgacgt gaggccgtc accaactac 
      ttcttagtg aacttatct gtggccgac ctcttagtg 
      gcactcgtc tgtatgccc aatgcggct tacgccgcc 
      tacacttcc acctacagc ttcggagag tacacctgc 
      aaaatatcg gcgtatttc caatgcgtc tcagtggcg 
      tcgagcatc ttcaccatc accgccatg tccatcgac 
      cggtacctg gccatcacc aagcccttc ggcttcttc 
      aaccgctgc ttcaacaag cgcaacacc gtcatcgtc 
      atcctcgtc ctgtggctc ttgtccttc gtcctcttc 
      ctgcccatc ctgctggtg accggcacg tggaaaatg 
      gacgacatc cacctcttc aacaagagc ctggacatc 
      ccggcctac ttctgccgc gaggactgg accacctac 
      ttcggctcc tcgggctcg gagatgcgc caagcgctc 
      ggcatcacc tggttcatc ttcatgttc gtggtgcca 
      gggatgata atgctgttc gcctactcg atgatgagc 
      aggacgctg tgctcgggg gttccgccc tttgacagc 
      aacgatggg ctgtcctgc atgcaacag agaaacaga 
      ctaatgagg agccgcaaa cgcgtggcc tgcatcctc 
      ctgctgctg gcgttcgtg ttcgccggc tgctggatg 
      ccctaccac ctggtgagc ctcctcgac gacctgaac 
      aacggcccc tacaagacc ctcaaaatg aacctagtg 
      ctcctcggc cacgccaat tccgccctc aaccccatc 
      atctactgc gccctttcg cgcaaattc cggcgctcc 
      atcaaagat ctgatttgc atcgatata catttcacc 
      agacgccgg caaaatatt cagtggggc gacagttcc 
      ggctcgggc acacaactc cattactta cacaggatt 
      aaatcagtg ccaactgtg cataatcag cttcatttc 
      acgctcgga cgcctgcag tcaagccag aaaacgacc 
      aagacgtgc gtggtttga
    mRNA sequence
    • >TC009184 RA
      agtcctcga aacgtcttt gacccaagt gttgtgcta 
      gtggagttg cagacgatg ggcacggga acatgggcc 
      aaccatggt ctgcggcgg acttttgga aaaatctaa 
      tcgagtgaa gtgaaaaaa gtcatgaat cactcaaat 
      gagaccgaa ctgaccgtc gaagatgtg ctgcgctcg 
      ttccgggaa gataatttt gttgtgatt ggactttat 
      gtgccggtg tttgttgtc gcgctgttg gccaatgtg 
      ctcgttatt tgggtcgtc atcaaggac cagtatatg 
      agaagcgtc accaactac ttcttagtg aacttatct 
      gtggccgac ctcttagtg gcactcgtc tgtatgccc 
      aatgcggct tacgccgcc tacacttcc acctacagc 
      ttcggagag tacacctgc aaaatatcg gcgtatttc 
      caatgcgtc tcagtggcg tcgagcatc ttcaccatc 
      accgccatg tccatcgac cggtacctg gccatcacc 
      aagcccttc ggcttcttc aaccgctgc ttcaacaag 
      cgcaacacc gtcatcgtc atcctcgtc ctgtggctc 
      ttgtccttc gtcctcttc ctgcccatc ctgctggtg 
      accggcacg tggaaaatg gacgacatc cacctcttc 
      aacaagagc ctggacatc ccggcctac ttctgccgc 
      gaggactgg accacctac ttcggctcc tcgggctcg 
      gagatgcgc caagcgctc ggcatcacc tggttcatc 
      ttcatgttc gtggtgcca gggatgata atgctgttc 
      gcctactcg atgatgagc aggacgctg tgctcgggg 
      gttccgccc tttgacagc aacgatggg ctgtcctgc 
      atgcaacag agaaacaga ctaatgagg agccgcaaa 
      cgcgtggcc tgcatcctc ctgctgctg gcgttcgtg 
      ttcgccggc tgctggatg ccctaccac ctggtgagc 
      ctcctcgac gacctgaac aacggcccc tacaagacc 
      ctcaaaatg aacctagtg ctcctcggc cacgccaat 
      tccgccctc aaccccatc atctactgc gccctttcg 
      cgcaaattc cggcgctcc atcaaagat ctgatttgc 
      atcgatata catttcacc agacgccgg caaaatatt 
      cagtggggc gacagttcc ggctcgggc acacaactc 
      cattactta cacaggatt aaatcagtg ccaactgtg 
      cataatcag cttcatttc acgctcgga cgcctgcag 
      tcaagccag aaaacgacc aagacgtgc gtggtttga 
      acacccaag ccctggttc aatacttgt gtgggacac 
      cctgtacat aagcccgtt ttgtttcag aaaaatcga 
      aaaatttgg gctcaaaca gtcaggttt ttcgattcg 
      tcgaccata tcacgtatt tttataccg gcctagctg 
      tagttttaa gacttttgt gtaaaaatg tttaatgtt 
      atacagggt gagtcattt gtgcggcgt ccgaaaata 
      ccgactttc ttaacaaaa atcactaaa ctatttgtt 
      tttgtcata cggggtgtt ttggataag tgatttaat 
      tttttgcac tagaatttg caataagga agtgggtat 
      cgtccaggg ctgctttat gccccaccc tgtagataa 
      gcaagatac gctttgcat ttccaactc ggcctctgg 
      cctctcatc atcaccatt cttgctact ctctgttcg 
      atgttcatg tctgtgatt ttcttaatg tatttctgt 
      tattactat tacttaata aatatgttt ttttatt
    • >TC009184 RB
      agtcctcga aacgtcttt gacccaagt gttgtgcta 
      gtggagttg cagacgatg ggcacggga acatgggcc 
      aaccatggt ctgcggcgg acttttgga aaaatctaa 
      tcgagtgaa gtgaaaaaa gtcatgaat cactcaaat 
      gagaccgaa ctgaccgtc gaagatgtg ctgcgctcg 
      ttccgggaa gataatttt gttgtgatt ggactttat 
      gtgccggtg tttgttgtc gcgctgttg gccaatgtg 
      ctcgttatt tgggtcgtc atcaaggac cagtatatg 
      agaaggttt ctgtggtgt tacaacagc atttcgcga 
      cgtgaggcc gtcaccaac tacttctta gtgaactta 
      tctgtggcc gacctctta gtggcactc gtctgtatg 
      cccaatgcg gcttacgcc gcctacact tccacctac 
      agcttcgga gagtacacc tgcaaaata tcggcgtat 
      ttccaatgc gtctcagtg gcgtcgagc atcttcacc 
      atcaccgcc atgtccatc gaccggtac ctggccatc 
      accaagccc ttcggcttc ttcaaccgc tgcttcaac 
      aagcgcaac accgtcatc gtcatcctc gtcctgtgg 
      ctcttgtcc ttcgtcctc ttcctgccc atcctgctg 
      gtgaccggc acgtggaaa atggacgac atccacctc 
      ttcaacaag agcctggac atcccggcc tacttctgc 
      cgcgaggac tggaccacc tacttcggc tcctcgggc 
      tcggagatg cgccaagcg ctcggcatc acctggttc 
      atcttcatg ttcgtggtg ccagggatg ataatgctg 
      ttcgcctac tcgatgatg agcaggacg ctgtgctcg 
      ggggttccg ccctttgac agcaacgat gggctgtcc 
      tgcatgcaa cagagaaac agactaatg aggagccgc 
      aaacgcgtg gcctgcatc ctcctgctg ctggcgttc 
      gtgttcgcc ggctgctgg atgccctac cacctggtg 
      agcctcctc gacgacctg aacaacggc ccctacaag 
      accctcaaa atgaaccta gtgctcctc ggccacgcc 
      aattccgcc ctcaacccc atcatctac tgcgccctt 
      tcgcgcaaa ttccggcgc tccatcaaa gatctgatt 
      tgcatcgat atacatttc accagacgc cggcaaaat 
      attcagtgg ggcgacagt tccggctcg ggcacacaa 
      ctccattac ttacacagg attaaatca gtgccaact 
      gtgcataat cagcttcat ttcacgctc ggacgcctg 
      cagtcaagc cagaaaacg accaagacg tgcgtggtt 
      tgaacaccc aagccctgg ttcaatact tgtgtggga 
      caccctgta cataagccc gttttgttt cagaaaaat 
      cgaaaaatt tgggctcaa acagtcagg tttttcgat 
      tcgtcgacc atatcacgt atttttata ccggcctag 
      ctgtagttt taagacttt tgtgtaaaa atgtttaat 
      gttatacag ggtgagtca tttgtgcgg cgtccgaaa 
      ataccgact ttcttaaca aaaatcact aaactattt 
      gtttttgtc atacggggt gttttggat aagtgattt 
      aattttttg cactagaat ttgcaataa ggaagtggg 
      tatcgtcca gggctgctt tatgcccca ccctgtaga 
      taagcaaga tacgctttg catttccaa ctcggcctc 
      tggcctctc atcatcacc attcttgct actctctgt 
      tcgatgttc atgtctgtg attttctta atgtatttc 
      tgttattac tattactta ataaatatg tttttttat 
      t
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