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Gene information
General information
TC009151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3745728..3785955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009151
    iBeetle screen iB_04599

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009151 RA
      MSGTNQAIP EGVNPENNH VGGGPLINT NRIRNNNNQ 
      NPLFNVRDR LFHTLFFKV ALVYARTFP KPVRRFFEF 
      IILIKAIAA FFVLVYIHT AFSKTPTTC LQHVKDTWP 
      RDGILRVEI VRNVGQDYN IEQSYAREE KLKQEKVDD 
      ISNVLGLLA RDGFVNIEP SAVEETEPL SQNYINESK 
      NESKKPNVL FNVSQISST IWDGFQRLT ELPSISSVA 
      FPQETQSTE VGQNEQSTT KKESSKSDW SSDEYIVEY 
      SLEYGFLRL SPMTRARLK IPVQIVTLD PGKDECFGD 
      AFSRLILDE FLGYDDLLM ASIKTLAEQ EDNKGYLRN 
      VVTGEHYRF VSMWMARTS YFAAFFIMI VFTVSVSML 
      LRYSHHQIF VFIVDLLQM LEFNVTVSF PAAPLLTVI 
      LALVGMEAI MSEFFNDTT TAFYIILIV WMADQYDAI 
      CCHTAITKR HWLRFFYLY HFSFYAYHY RFNGQYSSL 
      ALVTSWLFI QHSMLYFFH HYELPVILQ QAHFQQFII 
      RNSQQQQQQ QQQREPLAA AAPQNTITV RLTELFSAV 
      RPSATTQTS TSQVTTSTT TTATVGTSA QPNTSSQTS 
      QTTPAAVES AAAQTPPGA ETAVAAIQT SEGHRAPTG 
      GPDHH
    CDS sequence
    • >TC009151 RA
      atgtctgga acgaatcag gctatcccc gaaggggtc 
      aaccccgag aacaaccac gtcggaggc ggccccctt 
      atcaacaca aataggatt aggaataat aacaatcag 
      aacccgctt tttaacgtg cgcgaccgc ttatttcat 
      actttattc ttcaaagtg gcgctagtt tacgctagg 
      acctttcca aagcctgtt agacgattt ttcgaattt 
      atcatttta atcaaggcg atagccgcg ttttttgta 
      ctcgtgtat atacatacc gcgttttcc aaaaccccg 
      accacttgc ttgcaacac gtgaaagac acgtggccc 
      cgcgatggc attctgcgg gtggagatt gtgcgaaat 
      gttggccaa gactacaac attgagcag agctacgcc 
      agagaggag aaactgaag caggagaag gtcgacgat 
      atttcgaac gttttgggg cttttggcc agggacggc 
      tttgttaat atcgagccc tcggccgtt gaagagact 
      gaacctttg agccaaaat tatattaat gagagtaaa 
      aacgagtca aagaaaccc aatgttctc ttcaacgtg 
      tcgcagatt tcctcgacc atttgggac gggtttcag 
      cgcctcact gaattaccc tccatttcg tcggttgct 
      ttcccgcaa gaaacgcaa tcgacggaa gtcggccaa 
      aacgagcaa agcaccacg aagaaggaa tcgagcaaa 
      tcagactgg tcgagcgac gaatacatc gttgagtat 
      tcgctggaa tacggcttt ttgcgtttg tcgccgatg 
      acaagggca cgactgaaa attccggtg cagattgtg 
      acgctggat ccgggcaag gatgagtgt tttggcgat 
      gctttcagt cgactgatt ttagacgag tttttgggt 
      tatgatgat ttgctcatg gcttcgatt aaaactttg 
      gccgaacaa gaggacaat aaaggatat ctcagaaat 
      gtcgtaact ggggaacat taccggttt gtgagtatg 
      tggatggcg aggacgtcg tattttgct gcctttttc 
      ataatgatc gtctttacg gtgtccgtg tccatgctg 
      ctgcgctat tcgcatcac cagatattc gtgttcatc 
      gttgatttg ttgcaaatg ctggagttc aacgtgact 
      gtaagtttt cccgcggct cctctactg accgtcatc 
      ctggcttta gttggaatg gaggcgatt atgtccgaa 
      tttttcaac gacacgacg accgcattt tacatcatt 
      ttgatcgtc tggatggcc gaccaatac gacgccatc 
      tgttgtcac acggccatt acgaaacgc cactggtta 
      aggttcttc tatttgtac cacttttcc ttctacgcc 
      taccactat cgattcaac ggacagtat agcagtctt 
      gcattggtc acttcgtgg cttttcata cagcattca 
      atgctctac tttttccac cactacgag ctgccggtg 
      atcctgcag caggcccac ttccagcag ttcataatc 
      cgcaacagc cagcagcag cagcagcag cagcagcag 
      cgcgagcct ctggcggcg gccgccccg cagaacacc 
      atcacagtc cggctcacc gagctgttc tccgccgtg 
      cggccgtcg gccaccacc cagacgtcc acgagccag 
      gtgacgacg tccacgacc accaccgcc acggtgggc 
      acgtcggcg cagcccaac acctcgagc cagaccagc 
      cagacgacg ccggcggcg gtggagtcg gcggcggca 
      cagacgccg ccgggggcg gagacggcc gtggcggcc 
      atccagacc agcgagggg cacagggcg cccacaggg 
      gggcccgac caccactga
    mRNA sequence
    • >TC009151 RA
      tgttggttc aaatctgtg ctttttatg aataaatat 
      caaaatata aataaataa aacgcaggt ggaccttta 
      tacagtcgc aaaaatccc accgttatg tctttggac 
      gcctttaaa cgcccacca aacctctaa cctccgtgc 
      aaaacaaga atgatgcaa attggctga gccatgtct 
      ggaacgaat caggctatc cccgaaggg gtcaacccc 
      gagaacaac cacgtcgga ggcggcccc cttatcaac 
      acaaatagg attaggaat aataacaat cagaacccg 
      ctttttaac gtgcgcgac cgcttattt catacttta 
      ttcttcaaa gtggcgcta gtttacgct aggaccttt 
      ccaaagcct gttagacga tttttcgaa tttatcatt 
      ttaatcaag gcgatagcc gcgtttttt gtactcgtg 
      tatatacat accgcgttt tccaaaacc ccgaccact 
      tgcttgcaa cacgtgaaa gacacgtgg ccccgcgat 
      ggcattctg cgggtggag attgtgcga aatgttggc 
      caagactac aacattgag cagagctac gccagagag 
      gagaaactg aagcaggag aaggtcgac gatatttcg 
      aacgttttg gggcttttg gccagggac ggctttgtt 
      aatatcgag ccctcggcc gttgaagag actgaacct 
      ttgagccaa aattatatt aatgagagt aaaaacgag 
      tcaaagaaa cccaatgtt ctcttcaac gtgtcgcag 
      atttcctcg accatttgg gacgggttt cagcgcctc 
      actgaatta ccctccatt tcgtcggtt gctttcccg 
      caagaaacg caatcgacg gaagtcggc caaaacgag 
      caaagcacc acgaagaag gaatcgagc aaatcagac 
      tggtcgagc gacgaatac atcgttgag tattcgctg 
      gaatacggc tttttgcgt ttgtcgccg atgacaagg 
      gcacgactg aaaattccg gtgcagatt gtgacgctg 
      gatccgggc aaggatgag tgttttggc gatgctttc 
      agtcgactg attttagac gagtttttg ggttatgat 
      gatttgctc atggcttcg attaaaact ttggccgaa 
      caagaggac aataaagga tatctcaga aatgtcgta 
      actggggaa cattaccgg tttgtgagt atgtggatg 
      gcgaggacg tcgtatttt gctgccttt ttcataatg 
      atcgtcttt acggtgtcc gtgtccatg ctgctgcgc 
      tattcgcat caccagata ttcgtgttc atcgttgat 
      ttgttgcaa atgctggag ttcaacgtg actgtaagt 
      tttcccgcg gctcctcta ctgaccgtc atcctggct 
      ttagttgga atggaggcg attatgtcc gaatttttc 
      aacgacacg acgaccgca ttttacatc attttgatc 
      gtctggatg gccgaccaa tacgacgcc atctgttgt 
      cacacggcc attacgaaa cgccactgg ttaaggttc 
      ttctatttg taccacttt tccttctac gcctaccac 
      tatcgattc aacggacag tatagcagt cttgcattg 
      gtcacttcg tggcttttc atacagcat tcaatgctc 
      tactttttc caccactac gagctgccg gtgatcctg 
      cagcaggcc cacttccag cagttcata atccgcaac 
      agccagcag cagcagcag cagcagcag cagcgcgag 
      cctctggcg gcggccgcc ccgcagaac accatcaca 
      gtccggctc accgagctg ttctccgcc gtgcggccg 
      tcggccacc acccagacg tccacgagc caggtgacg 
      acgtccacg accaccacc gccacggtg ggcacgtcg 
      gcgcagccc aacacctcg agccagacc agccagacg 
      acgccggcg gcggtggag tcggcggcg gcacagacg 
      ccgccgggg gcggagacg gccgtggcg gccatccag 
      accagcgag gggcacagg gcgcccaca ggggggccc 
      gaccaccac tgagacatg tacatactt agtcggttt 
      ctgctttaa gagtgtata taattttgt cggtggatg 
      taggatgtg tacttgatt gaaattact aaataaagt 
      ttcgagtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY