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Gene information
General information
TC009151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3745728..3785955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009151
    iBeetle screen iB_04599

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009151 RA
      MSGTNQAIP EGVNPENNH VGGGPLINT NRIRNNNNQ 
NPLFNVRDR LFHTLFFKV ALVYARTFP KPVRRFFEF 
IILIKAIAA FFVLVYIHT AFSKTPTTC LQHVKDTWP 
RDGILRVEI VRNVGQDYN IEQSYAREE KLKQEKVDD 
ISNVLGLLA RDGFVNIEP SAVEETEPL SQNYINESK 
NESKKPNVL FNVSQISST IWDGFQRLT ELPSISSVA 
FPQETQSTE VGQNEQSTT KKESSKSDW SSDEYIVEY 
SLEYGFLRL SPMTRARLK IPVQIVTLD PGKDECFGD 
AFSRLILDE FLGYDDLLM ASIKTLAEQ EDNKGYLRN 
VVTGEHYRF VSMWMARTS YFAAFFIMI VFTVSVSML 
LRYSHHQIF VFIVDLLQM LEFNVTVSF PAAPLLTVI 
LALVGMEAI MSEFFNDTT TAFYIILIV WMADQYDAI 
CCHTAITKR HWLRFFYLY HFSFYAYHY RFNGQYSSL 
ALVTSWLFI QHSMLYFFH HYELPVILQ QAHFQQFII 
RNSQQQQQQ QQQREPLAA AAPQNTITV RLTELFSAV 
RPSATTQTS TSQVTTSTT TTATVGTSA QPNTSSQTS 
QTTPAAVES AAAQTPPGA ETAVAAIQT SEGHRAPTG 
GPDHH
    CDS sequence
    • >TC009151 RA
      atgtctgga acgaatcag gctatcccc gaaggggtc 
aaccccgag aacaaccac gtcggaggc ggccccctt 
atcaacaca aataggatt aggaataat aacaatcag 
aacccgctt tttaacgtg cgcgaccgc ttatttcat 
actttattc ttcaaagtg gcgctagtt tacgctagg 
acctttcca aagcctgtt agacgattt ttcgaattt 
atcatttta atcaaggcg atagccgcg ttttttgta 
ctcgtgtat atacatacc gcgttttcc aaaaccccg 
accacttgc ttgcaacac gtgaaagac acgtggccc 
cgcgatggc attctgcgg gtggagatt gtgcgaaat 
gttggccaa gactacaac attgagcag agctacgcc 
agagaggag aaactgaag caggagaag gtcgacgat 
atttcgaac gttttgggg cttttggcc agggacggc 
tttgttaat atcgagccc tcggccgtt gaagagact 
gaacctttg agccaaaat tatattaat gagagtaaa 
aacgagtca aagaaaccc aatgttctc ttcaacgtg 
tcgcagatt tcctcgacc atttgggac gggtttcag 
cgcctcact gaattaccc tccatttcg tcggttgct 
ttcccgcaa gaaacgcaa tcgacggaa gtcggccaa 
aacgagcaa agcaccacg aagaaggaa tcgagcaaa 
tcagactgg tcgagcgac gaatacatc gttgagtat 
tcgctggaa tacggcttt ttgcgtttg tcgccgatg 
acaagggca cgactgaaa attccggtg cagattgtg 
acgctggat ccgggcaag gatgagtgt tttggcgat 
gctttcagt cgactgatt ttagacgag tttttgggt 
tatgatgat ttgctcatg gcttcgatt aaaactttg 
gccgaacaa gaggacaat aaaggatat ctcagaaat 
gtcgtaact ggggaacat taccggttt gtgagtatg 
tggatggcg aggacgtcg tattttgct gcctttttc 
ataatgatc gtctttacg gtgtccgtg tccatgctg 
ctgcgctat tcgcatcac cagatattc gtgttcatc 
gttgatttg ttgcaaatg ctggagttc aacgtgact 
gtaagtttt cccgcggct cctctactg accgtcatc 
ctggcttta gttggaatg gaggcgatt atgtccgaa 
tttttcaac gacacgacg accgcattt tacatcatt 
ttgatcgtc tggatggcc gaccaatac gacgccatc 
tgttgtcac acggccatt acgaaacgc cactggtta 
aggttcttc tatttgtac cacttttcc ttctacgcc 
taccactat cgattcaac ggacagtat agcagtctt 
gcattggtc acttcgtgg cttttcata cagcattca 
atgctctac tttttccac cactacgag ctgccggtg 
atcctgcag caggcccac ttccagcag ttcataatc 
cgcaacagc cagcagcag cagcagcag cagcagcag 
cgcgagcct ctggcggcg gccgccccg cagaacacc 
atcacagtc cggctcacc gagctgttc tccgccgtg 
cggccgtcg gccaccacc cagacgtcc acgagccag 
gtgacgacg tccacgacc accaccgcc acggtgggc 
acgtcggcg cagcccaac acctcgagc cagaccagc 
cagacgacg ccggcggcg gtggagtcg gcggcggca 
cagacgccg ccgggggcg gagacggcc gtggcggcc 
atccagacc agcgagggg cacagggcg cccacaggg 
gggcccgac caccactga
    mRNA sequence
    • >TC009151 RA
      tgttggttc aaatctgtg ctttttatg aataaatat 
caaaatata aataaataa aacgcaggt ggaccttta 
tacagtcgc aaaaatccc accgttatg tctttggac 
gcctttaaa cgcccacca aacctctaa cctccgtgc 
aaaacaaga atgatgcaa attggctga gccatgtct 
ggaacgaat caggctatc cccgaaggg gtcaacccc 
gagaacaac cacgtcgga ggcggcccc cttatcaac 
acaaatagg attaggaat aataacaat cagaacccg 
ctttttaac gtgcgcgac cgcttattt catacttta 
ttcttcaaa gtggcgcta gtttacgct aggaccttt 
ccaaagcct gttagacga tttttcgaa tttatcatt 
ttaatcaag gcgatagcc gcgtttttt gtactcgtg 
tatatacat accgcgttt tccaaaacc ccgaccact 
tgcttgcaa cacgtgaaa gacacgtgg ccccgcgat 
ggcattctg cgggtggag attgtgcga aatgttggc 
caagactac aacattgag cagagctac gccagagag 
gagaaactg aagcaggag aaggtcgac gatatttcg 
aacgttttg gggcttttg gccagggac ggctttgtt 
aatatcgag ccctcggcc gttgaagag actgaacct 
ttgagccaa aattatatt aatgagagt aaaaacgag 
tcaaagaaa cccaatgtt ctcttcaac gtgtcgcag 
atttcctcg accatttgg gacgggttt cagcgcctc 
actgaatta ccctccatt tcgtcggtt gctttcccg 
caagaaacg caatcgacg gaagtcggc caaaacgag 
caaagcacc acgaagaag gaatcgagc aaatcagac 
tggtcgagc gacgaatac atcgttgag tattcgctg 
gaatacggc tttttgcgt ttgtcgccg atgacaagg 
gcacgactg aaaattccg gtgcagatt gtgacgctg 
gatccgggc aaggatgag tgttttggc gatgctttc 
agtcgactg attttagac gagtttttg ggttatgat 
gatttgctc atggcttcg attaaaact ttggccgaa 
caagaggac aataaagga tatctcaga aatgtcgta 
actggggaa cattaccgg tttgtgagt atgtggatg 
gcgaggacg tcgtatttt gctgccttt ttcataatg 
atcgtcttt acggtgtcc gtgtccatg ctgctgcgc 
tattcgcat caccagata ttcgtgttc atcgttgat 
ttgttgcaa atgctggag ttcaacgtg actgtaagt 
tttcccgcg gctcctcta ctgaccgtc atcctggct 
ttagttgga atggaggcg attatgtcc gaatttttc 
aacgacacg acgaccgca ttttacatc attttgatc 
gtctggatg gccgaccaa tacgacgcc atctgttgt 
cacacggcc attacgaaa cgccactgg ttaaggttc 
ttctatttg taccacttt tccttctac gcctaccac 
tatcgattc aacggacag tatagcagt cttgcattg 
gtcacttcg tggcttttc atacagcat tcaatgctc 
tactttttc caccactac gagctgccg gtgatcctg 
cagcaggcc cacttccag cagttcata atccgcaac 
agccagcag cagcagcag cagcagcag cagcgcgag 
cctctggcg gcggccgcc ccgcagaac accatcaca 
gtccggctc accgagctg ttctccgcc gtgcggccg 
tcggccacc acccagacg tccacgagc caggtgacg 
acgtccacg accaccacc gccacggtg ggcacgtcg 
gcgcagccc aacacctcg agccagacc agccagacg 
acgccggcg gcggtggag tcggcggcg gcacagacg 
ccgccgggg gcggagacg gccgtggcg gccatccag 
accagcgag gggcacagg gcgcccaca ggggggccc 
gaccaccac tgagacatg tacatactt agtcggttt 
ctgctttaa gagtgtata taattttgt cggtggatg 
taggatgtg tacttgatt gaaattact aaataaagt 
ttcgagtc
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