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Gene information
General information
TC033639
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009123
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4719810..4724807
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033639
    iBeetle screen iB_04596

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 3

    wings distal bent - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033639 RA
      MQFPEKQLK TNHAPSTTA EKDVAALKA ELEIELKNK 
      KQCTYKPYE CTQLTLDGF NYCLKHILQ DKAAPYRQC 
      SYIYASNGK RCQLAAPKI EKKDCGYCN DHAVKVTLE 
      KNRQNSRLQ PPQTAEVLI YSLGHYLKK PRNRATSGS 
      GEEAERALL EEAEAKSTK CLDPFRDID ANALYNSTC 
      NNVLDFCSE SDSDVEAST FASVWHDAQ NDSSDNESV 
      DSDQEDVLK HANVYTAEE ISSITREKL IKLQSLYIE 
      QYRHLQHVF KECKRKYVY NLRREKETC CNIYNQIRE 
      NPKEQRLYK KLKALNKYH KPHGVEAIL HKREHDSRA 
      KITDGMFSK APGYSRCLF TEGGVKCGE RTLPLARHC 
      RKHILEDQN QVLFRPCGK KRADVECKT PVEAIFEDS 
      CCNLHMDIP PIKSYAQLR KNSESDFED SFESPENML 
      MEAVSESDN VKTEFIDYG PNLEEMKTL PSVLFEESS 
      MDASIKNED TENEKPDED VQIDVDDEP SPEGKLEID 
      IPEEDTNDI RIEDKMEED SSQDKPAPE PPVTDN
    • >TC033639 RB
      MADQPRMQF PEKQLKTNH APSTTAEKD VAALKAELE 
      IELKNKKQC TYKPYECTQ LTLDGFNYC LKHILQDKA 
      APYRQCSYI YASNGKRCQ LAAPKIEKK DCGYCNDHA 
      VKVTLEKNR QNSRLQPPQ TAEVLIYSL GHYLKKPRN 
      RATSGSGEE AERALLEEA EAKSTKCLD PFRDIDANA 
      LYNSTCNNV LDFCSESDS DVEASTFAS VWHDAQNDS 
      SDNESVDSD QEDVLKHAN VYTAEEISS ITREKLIKL 
      QSLYIEQYR HLQHVFKEC KRKYVYNLR REKETCCNI 
      YNQIRENPK EQRLYKKLK ALNKYHKPH GVEAILHKR 
      EHDSRAKIT DGMFSKAPG YSRCLFTEG GVKCGERTL 
      PLARHCRKH ILEDQNQVL FRPCGKKRA DVECKTPVE 
      AIFEDSCCN LHMDIPPIK SYAQLRKNS ESDFEDSFE 
      SPENMLMEA VSESDNVKT EFIDYGPNL EEMKTLPSV 
      LFEESSMDA SIKNEDTEN EKPDEDVQI DVDDEPSPE 
      GKLEIDIPE EDTNDIRIE DKMEEDSSQ DKPAPEPPV 
      TDN
    CDS sequence
    • >TC033639 RA
      atgcagttt ccggaaaaa caactcaaa accaaccat 
      gccccttca acgactgca gaaaaagac gtcgccgcc 
      ctcaaagcc gagctagaa atcgagctc aagaacaaa 
      aagcaatgc acatacaaa ccgtacgaa tgcacccaa 
      ctcaccctc gacgggttc aattactgc ctcaaacac 
      atcctccaa gacaaggca gccccctac agacagtgc 
      tcgtacatt tacgccagt aacgggaaa cgctgccag 
      ctggcagcc cccaaaatc gaaaagaaa gactgcggc 
      tactgtaac gatcacgca gttaaagtc acacttgag 
      aaaaacagg cagaactcg aggcttcag ccgccacaa 
      accgcagaa gtcctgata tattcgttg ggacattac 
      ttgaagaag cccagaaat cgggcaact tctggtagt 
      ggagaggag gcagagagg gcccttctg gaggaggct 
      gaagccaag agcaccaag tgcttggac ccgttccgt 
      gatattgac gccaatgcc ttgtataac tccacttgt 
      aacaacgtt ttggacttc tgtagtgag tctgatagc 
      gatgttgag gcttcaacg ttcgcgtca gtgtggcat 
      gatgcacag aatgacagt tccgataac gaaagtgtt 
      gattcagac caggaggac gttttaaag cacgcgaat 
      gtgtacaca gctgaagaa atcagttca attacgaga 
      gaaaagtta atcaagctt cagtcgtta tacattgag 
      caatatcgt catttgcag catgtcttt aaggaatgc 
      aaaagaaaa tatgtttat aatttaagg agggaaaag 
      gaaacttgc tgcaatatt tataaccag atccgtgaa 
      aatcccaaa gaacagcgg ctttacaaa aaactcaaa 
      gcgctaaat aagtaccac aaaccgcac ggagttgaa 
      gcgatactt cataaaaga gagcatgat tcacgcgct 
      aagattaca gatggcatg ttctccaaa gcgccaggt 
      tactcgcgg tgtttgttt acagaaggt ggggtcaag 
      tgtggggag agaactctg cctctggct agacattgc 
      aggaaacat attttagaa gatcaaaat caagtatta 
      ttcaggccg tgcggtaag aagagagcg gatgtagaa 
      tgtaaaaca ccagttgag gcgattttt gaggacagt 
      tgctgcaac ttgcatatg gacatacct ccaattaaa 
      tcatatgcg caattaagg aaaaattct gaatccgac 
      tttgaggat tcctttgaa tctcccgaa aacatgttg 
      atggaagcg gtgtcagag tccgacaac gtgaagaca 
      gaatttata gactacggc cccaacttg gaagaaatg 
      aagaccttg ccgtctgtt ttatttgaa gagtccagc 
      atggacgct agtattaaa aacgaagac acggagaac 
      gaaaaaccg gatgaagat gtacaaatc gacgtagac 
      gacgaacct agtcccgaa ggaaaacta gaaattgat 
      atacctgaa gaagatacc aatgatatt agaatcgaa 
      gataagatg gaagaagac agttcgcag gataaaccc 
      gccccggag cccccagtt actgataat tag
    • >TC033639 RB
      atggccgac caaccgcgg atgcagttt ccggaaaaa 
      caactcaaa accaaccat gccccttca acgactgca 
      gaaaaagac gtcgccgcc ctcaaagcc gagctagaa 
      atcgagctc aagaacaaa aagcaatgc acatacaaa 
      ccgtacgaa tgcacccaa ctcaccctc gacgggttc 
      aattactgc ctcaaacac atcctccaa gacaaggca 
      gccccctac agacagtgc tcgtacatt tacgccagt 
      aacgggaaa cgctgccag ctggcagcc cccaaaatc 
      gaaaagaaa gactgcggc tactgtaac gatcacgca 
      gttaaagtc acacttgag aaaaacagg cagaactcg 
      aggcttcag ccgccacaa accgcagaa gtcctgata 
      tattcgttg ggacattac ttgaagaag cccagaaat 
      cgggcaact tctggtagt ggagaggag gcagagagg 
      gcccttctg gaggaggct gaagccaag agcaccaag 
      tgcttggac ccgttccgt gatattgac gccaatgcc 
      ttgtataac tccacttgt aacaacgtt ttggacttc 
      tgtagtgag tctgatagc gatgttgag gcttcaacg 
      ttcgcgtca gtgtggcat gatgcacag aatgacagt 
      tccgataac gaaagtgtt gattcagac caggaggac 
      gttttaaag cacgcgaat gtgtacaca gctgaagaa 
      atcagttca attacgaga gaaaagtta atcaagctt 
      cagtcgtta tacattgag caatatcgt catttgcag 
      catgtcttt aaggaatgc aaaagaaaa tatgtttat 
      aatttaagg agggaaaag gaaacttgc tgcaatatt 
      tataaccag atccgtgaa aatcccaaa gaacagcgg 
      ctttacaaa aaactcaaa gcgctaaat aagtaccac 
      aaaccgcac ggagttgaa gcgatactt cataaaaga 
      gagcatgat tcacgcgct aagattaca gatggcatg 
      ttctccaaa gcgccaggt tactcgcgg tgtttgttt 
      acagaaggt ggggtcaag tgtggggag agaactctg 
      cctctggct agacattgc aggaaacat attttagaa 
      gatcaaaat caagtatta ttcaggccg tgcggtaag 
      aagagagcg gatgtagaa tgtaaaaca ccagttgag 
      gcgattttt gaggacagt tgctgcaac ttgcatatg 
      gacatacct ccaattaaa tcatatgcg caattaagg 
      aaaaattct gaatccgac tttgaggat tcctttgaa 
      tctcccgaa aacatgttg atggaagcg gtgtcagag 
      tccgacaac gtgaagaca gaatttata gactacggc 
      cccaacttg gaagaaatg aagaccttg ccgtctgtt 
      ttatttgaa gagtccagc atggacgct agtattaaa 
      aacgaagac acggagaac gaaaaaccg gatgaagat 
      gtacaaatc gacgtagac gacgaacct agtcccgaa 
      ggaaaacta gaaattgat atacctgaa gaagatacc 
      aatgatatt agaatcgaa gataagatg gaagaagac 
      agttcgcag gataaaccc gccccggag cccccagtt 
      actgataat tag
    mRNA sequence
    • >TC033639 RA
      ttttttact cttgtgaaa gattacaat tattttaca 
      tcatacaaa tcgcaaatg aaattgttg cataagaat 
      gacaaaact actacaaaa attgaaaaa ttgcttcaa 
      agagaaaat aaaaaaatg aataaaacc gctgaccta 
      caacaaacg tagagaaga gttaaataa taaattttc 
      tggaaaaga atacgttga aataatcct gaaaagcat 
      aaaaataac ggtgcgttt aacacacgt gtcgtgtga 
      acgccgctt tatcgcatt tgcatgaaa cgtcaaata 
      tgcgcagta gagggggct atctacgtc acttacgaa 
      acatggcgc aatagctcc acaatgacc taaaacaag 
      ggtcgtggg gcgccaggg ggctgaaat tcgcgctat 
      ctacgtgtt aaatgcaag catgcggca aggatacac 
      tttgagcct ctgtcccat gtcctggat gaaattggc 
      agtctgctg cctgtagga cagctcctt gtagctatc 
      atatccgca tttcctcga gggcgtagc gtccaattg 
      ctctttcgt gaagtacca tctatcgct gttattagc 
      catttactg cactcgaca cgactcgcg ataacagca 
      aggcaagtg tcgctacgg ctcgaaatc aggctattc 
      caggtcatt tgctggtct tattggggc aaatggggg 
      cgcattgtc gcgaaattt agtcacatt aactacact 
      tttgtccag aaaatcggc ttcaagggt ttccgttgc 
      gtcactacg atgtgggtt ttcctagaa gtggcactg 
      gtgcaaatc gggtcggct tttcacggg caaatctcc 
      ggttgcaag tctcgaatt gatcacgaa ttattactt 
      aaagtttac taaacgctt ttggaaagt ttttcgcgc 
      gaaagaggt agttcttta acaaattaa aatatacat 
      acacaaagt tgttggaat ttgatgtta tttcactca 
      gagatggcc gaccaaccg cggatgcag tttccggaa 
      aaacaactc aaaaccaac catgcccct tcaacgact 
      gcagaaaaa gacgtcgcc gccctcaaa gccgagcta 
      gaaatcgag ctcaagaac aaaaagcaa tgcacatac 
      aaaccgtac gaatgcacc caactcacc ctcgacggg 
      ttcaattac tgcctcaaa cacatcctc caagacaag 
      gcagccccc tacagacag tgctcgtac atttacgcc 
      agtaacggg aaacgctgc cagctggca gcccccaaa 
      atcgaaaag aaagactgc ggctactgt aacgatcac 
      gcagttaaa gtcacactt gagaaaaac aggcagaac 
      tcgaggctt cagccgcca caaaccgca gaagtcctg 
      atatattcg ttgggacat tacttgaag aagcccaga 
      aatcgggca acttctggt agtggagag gaggcagag 
      agggccctt ctggaggag gctgaagcc aagagcacc 
      aagtgcttg gacccgttc cgtgatatt gacgccaat 
      gccttgtat aactccact tgtaacaac gttttggac 
      ttctgtagt gagtctgat agcgatgtt gaggcttca 
      acgttcgcg tcagtgtgg catgatgca cagaatgac 
      agttccgat aacgaaagt gttgattca gaccaggag 
      gacgtttta aagcacgcg aatgtgtac acagctgaa 
      gaaatcagt tcaattacg agagaaaag ttaatcaag 
      cttcagtcg ttatacatt gagcaatat cgtcatttg 
      cagcatgtc tttaaggaa tgcaaaaga aaatatgtt 
      tataattta aggagggaa aaggaaact tgctgcaat 
      atttataac cagatccgt gaaaatccc aaagaacag 
      cggctttac aaaaaactc aaagcgcta aataagtac 
      cacaaaccg cacggagtt gaagcgata cttcataaa 
      agagagcat gattcacgc gctaagatt acagatggc 
      atgttctcc aaagcgcca ggttactcg cggtgtttg 
      tttacagaa ggtggggtc aagtgtggg gagagaact 
      ctgcctctg gctagacat tgcaggaaa catatttta 
      gaagatcaa aatcaagta ttattcagg ccgtgcggt 
      aagaagaga gcggatgta gaatgtaaa acaccagtt 
      gaggcgatt tttgaggac agttgctgc aacttgcat 
      atggacata cctccaatt aaatcatat gcgcaatta 
      aggaaaaat tctgaatcc gactttgag gattccttt 
      gaatctccc gaaaacatg ttgatggaa gcggtgtca 
      gagtccgac aacgtgaag acagaattt atagactac 
      ggccccaac ttggaagaa atgaagacc ttgccgtct 
      gttttattt gaagagtcc agcatggac gctagtatt 
      aaaaacgaa gacacggag aacgaaaaa ccggatgaa 
      gatgtacaa atcgacgta gacgacgaa cctagtccc 
      gaaggaaaa ctagaaatt gatatacct gaagaagat 
      accaatgat attagaatc gaagataag atggaagaa 
      gacagttcg caggataaa cccgccccg gagccccca 
      gttactgat aattagttt taagttcca agtattatt 
      ttttgttac acgttttgt attcgcaat aaagccatt 
      tgaattaa
    • >TC033639 RB
      ctttttggt gttgaacag ttgatcaat gcttatctc 
      aaaccccat tacaattat tttacatca tacaaatcg 
      caaatgaaa ttgttgcat aagaatgac aaaactact 
      acaaaaatt gaaaaattg cttcaaaga gaaaataaa 
      aaaatgaat aaaaccgct gacctacaa caaacgtag 
      agaagagtt aaataataa attttctgg aaaagaata 
      cgttgaaat aatcctgaa aagcataaa aataacggt 
      gcgtttaac acacgtgtc gtgtgaacg ccgctttat 
      cgcatttgc atgaaacgt caaatatgc gcagtagag 
      ggggctatc tacgtcact tacgaaaca tggcgcaat 
      agctccaca atgacctaa aacaagggt cgtggggcg 
      ccagggggc tgaaattcg cgctatcta cgtgttaaa 
      tgcaagcat gcggcaagg atacacttt gagcctctg 
      tcccatgtc ctggatgaa attggcagt ctgctgcct 
      gtaggacag ctccttgta gctatcata tccgcattt 
      cctcgaggg cgtagcgtc caattgctc tttcgtgaa 
      gtaccatct atcgctgtt attagccat ttactgcac 
      tcgacacga ctcgcgata acagcaagg caagtgtcg 
      ctacggctc gaaatcagg ctattccag gtcatttgc 
      tggtcttat tggggcaaa tgggggcgc attgtcgcg 
      aaatttagt cacattaac tacactttt gtccagaaa 
      atcggcttc aagggtttc cgttgcgtc actacgatg 
      tgggttttc ctagaagtg gcactggtg caaatcggg 
      tcggctttt cacgggcaa atctccggt tgcaagtct 
      cgaattgat cacgaatta ttacttaaa gtttactaa 
      acgcttttg gaaagtttt tcgcgcgaa agaggtagt 
      tctttaaca aattaaaat atacataca caaagttgt 
      tggaatttg atgttattt cactcagag atggccgac 
      caaccgcgg atgcagttt ccggaaaaa caactcaaa 
      accaaccat gccccttca acgactgca gaaaaagac 
      gtcgccgcc ctcaaagcc gagctagaa atcgagctc 
      aagaacaaa aagcaatgc acatacaaa ccgtacgaa 
      tgcacccaa ctcaccctc gacgggttc aattactgc 
      ctcaaacac atcctccaa gacaaggca gccccctac 
      agacagtgc tcgtacatt tacgccagt aacgggaaa 
      cgctgccag ctggcagcc cccaaaatc gaaaagaaa 
      gactgcggc tactgtaac gatcacgca gttaaagtc 
      acacttgag aaaaacagg cagaactcg aggcttcag 
      ccgccacaa accgcagaa gtcctgata tattcgttg 
      ggacattac ttgaagaag cccagaaat cgggcaact 
      tctggtagt ggagaggag gcagagagg gcccttctg 
      gaggaggct gaagccaag agcaccaag tgcttggac 
      ccgttccgt gatattgac gccaatgcc ttgtataac 
      tccacttgt aacaacgtt ttggacttc tgtagtgag 
      tctgatagc gatgttgag gcttcaacg ttcgcgtca 
      gtgtggcat gatgcacag aatgacagt tccgataac 
      gaaagtgtt gattcagac caggaggac gttttaaag 
      cacgcgaat gtgtacaca gctgaagaa atcagttca 
      attacgaga gaaaagtta atcaagctt cagtcgtta 
      tacattgag caatatcgt catttgcag catgtcttt 
      aaggaatgc aaaagaaaa tatgtttat aatttaagg 
      agggaaaag gaaacttgc tgcaatatt tataaccag 
      atccgtgaa aatcccaaa gaacagcgg ctttacaaa 
      aaactcaaa gcgctaaat aagtaccac aaaccgcac 
      ggagttgaa gcgatactt cataaaaga gagcatgat 
      tcacgcgct aagattaca gatggcatg ttctccaaa 
      gcgccaggt tactcgcgg tgtttgttt acagaaggt 
      ggggtcaag tgtggggag agaactctg cctctggct 
      agacattgc aggaaacat attttagaa gatcaaaat 
      caagtatta ttcaggccg tgcggtaag aagagagcg 
      gatgtagaa tgtaaaaca ccagttgag gcgattttt 
      gaggacagt tgctgcaac ttgcatatg gacatacct 
      ccaattaaa tcatatgcg caattaagg aaaaattct 
      gaatccgac tttgaggat tcctttgaa tctcccgaa 
      aacatgttg atggaagcg gtgtcagag tccgacaac 
      gtgaagaca gaatttata gactacggc cccaacttg 
      gaagaaatg aagaccttg ccgtctgtt ttatttgaa 
      gagtccagc atggacgct agtattaaa aacgaagac 
      acggagaac gaaaaaccg gatgaagat gtacaaatc 
      gacgtagac gacgaacct agtcccgaa ggaaaacta 
      gaaattgat atacctgaa gaagatacc aatgatatt 
      agaatcgaa gataagatg gaagaagac agttcgcag 
      gataaaccc gccccggag cccccagtt actgataat 
      tagttttaa gttccaagt attattttt tgttacacg 
      ttttgtatt cgcaataaa gccatttga attaa
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