Status
Please wait ...
Gene information
General information
TC033642
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009117
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4816447..4822024
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033642
    iBeetle screen iB_04595

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033642 RA
      MSDMRLLDN KVSTTWCQR QLVKVNTTF NAFLLNNPP 
      TLSSQLKPL TMVRHLKLE DDTKEYWGF YLLQGSSVT 
      VSTCVRWPG ASLIVIRGH KHLHECAYI GDNSSEELD 
      ELMKAIQEK SYVPPKPKP KHNTTRTNE PEMMKRHRP 
      DVKFHHPVH KNSSESHTI KKAVDEADI TDPKMLQPI 
      LDSLQVKTK KKHKPTPEE NHMHVHRNS TIAKDETKN 
      IKIEAPHFG KTQSQEALD DIVQRLQGL GNKTNSILD 
      RLNEKFKNE GGNETNEQK FDVKGRHYS KPAVSFDEN 
      IDRARRRRQ ITISNAMKS NFTEDDEER NLAMEEGFV 
      PDGIADHRG TVNETTLND YSNSEFWSS FSSSEEALL 
      NCAGLILNL PLTPHHKCV PELTEEQAE ETYLANTIT 
      YQVPVNGYY FFVFNSENE VQPNYIRVQ FHLNKAVYN 
      VSNAVSTCL NSSAACAMD LKFFSSEKL VMELPVKEN 
      GNLWNEEFV VVSECEPRT AIYAICVIA VPVLVILFA 
      FS
    CDS sequence
    • >TC033642 RA
      atgtcagac atgaggctt ttggataac aaggtttca 
      acaacttgg tgtcaaaga caattagta aaagtcaac 
      acaactttc aatgcattc ctgttaaac aatccaccc 
      actttatca tcccaatta aaaccattg acaatggtt 
      cgccattta aagctagaa gatgatacg aaggaatac 
      tgggggttc tacctcttg caaggctct tccgtgaca 
      gtctcgacc tgtgtaaga tggcctggc gcctcttta 
      attgtgatc agaggccac aaacactta cacgaatgt 
      gcctacatt ggcgacaac tcctcggaa gaactcgac 
      gaattgatg aaagcaatt caggaaaaa agttacgtc 
      ccgcctaaa cccaagccc aaacataac accactcgc 
      actaacgag cccgaaatg atgaaacgg cacaggcca 
      gacgtgaaa tttcaccac cctgttcac aaaaacagc 
      agcgaaagc cacaccatt aaaaaagca gtggacgaa 
      gccgacatc actgatccc aaaatgctg caaccgatt 
      ttagattct cttcaagtg aaaacgaag aagaaacat 
      aaacctaca cctgaggag aaccacatg cacgtccac 
      agaaattcc acaattgcc aaagacgag accaaaaat 
      atcaaaata gaggcacca catttcggg aaaactcag 
      tcccaagag gcgctcgat gatatcgtt cagaggttg 
      caagggctg ggtaacaaa accaattcg attttggat 
      cggcttaat gagaagttt aaaaatgaa ggtggtaac 
      gaaacgaat gagcaaaag tttgatgtg aaagggagg 
      cattattca aaacctgcc gttagtttt gacgaaaat 
      atcgacaga gcgagaaga cgcaggcaa attacgatt 
      tcgaacgcc atgaagtcg aattttacg gaagatgat 
      gaggagcgg aatttagca atggaagaa ggttttgtc 
      cccgatggg atagccgac cacagaggc acagttaat 
      gaaacaact ctgaacgac tacagtaac agcgaattt 
      tggtcatct ttttcaagc tctgaggag gctctttta 
      aattgtgct ggtttaatt ctcaatctt ccactcacg 
      ccacatcac aagtgtgtc ccagaattg acggaagag 
      caagccgaa gaaacgtat cttgcaaat accatcact 
      taccaagtt cctgtgaat ggttactac tttttcgtc 
      ttcaacagt gaaaatgag gtacaaccg aactacatc 
      cgcgtccaa ttccaccta aataaggcc gtttataac 
      gtttcaaac gccgtatcg acttgcttg aacagttcc 
      gccgcttgt gcgatggac ctgaagttt ttctcctcg 
      gaaaaactg gtgatggaa ctaccagtt aaagaaaat 
      gggaatctt tggaatgaa gaattcgtt gtggtatct 
      gagtgtgaa ccacgaacc gctatttat gcaatatgt 
      gtgattgct gtacctgtt cttgtgata ctttttgca 
      ttttcgtga
    mRNA sequence
    • >TC033642 RA
      tccaactaa aattagaat tagttctcg actaaaagc 
      ggggagaat caccattga gccacagcg cgcgtttca 
      cgcaagata aagaaatcc aatgataaa agtttacag 
      aaaattctc acagataag tgttggtat tataaatat 
      gagtataca cctgtctgc gaggacttc ctaaacgtg 
      tgaatatta tgttattgt tgtttggtc gcggagaga 
      tatgttgga gtctggaac tttagttga agagtgtcc 
      tttaaaaaa tcacagtca gtgcaaaaa gcgtccaac 
      gaccctaaa aggcccatt ccgtttagt cagactatg 
      tgtattggg catttttct cccaagttt actgatagc 
      gcttcctct ttatatgag ataccatgt ctacggtca 
      ccaacttta tcctttggc aatgtcaga catgaggct 
      tttggataa caaggtttc aacaacttg gtgtcaaag 
      acaattagt aaaagtcaa cacaacttt caatgcatt 
      cctgttaaa caatccacc cactttatc atcccaatt 
      aaaaccatt gacaatggt tcgccattt aaagctaga 
      agatgatac gaaggaata ctgggggtt ctacctctt 
      gcaaggctc ttccgtgac agtctcgac ctgtgtaag 
      atggcctgg cgcctcttt aattgtgat cagaggcca 
      caaacactt acacgaatg tgcctacat tggcgacaa 
      ctcctcgga agaactcga cgaattgat gaaagcaat 
      tcaggaaaa aagttacgt cccgcctaa acccaagcc 
      caaacataa caccactcg cactaacga gcccgaaat 
      gatgaaacg gcacaggcc agacgtgaa atttcacca 
      ccctgttca caaaaacag cagcgaaag ccacaccat 
      taaaaaagc agtggacga agccgacat cactgatcc 
      caaaatgct gcaaccgat tttagattc tcttcaagt 
      gaaaacgaa gaagaaaca taaacctac acctgagga 
      gaaccacat gcacgtcca cagaaattc cacaattgc 
      caaagacga gaccaaaaa tatcaaaat agaggcacc 
      acatttcgg gaaaactca gtcccaaga ggcgctcga 
      tgatatcgt tcagaggtt gcaagggct gggtaacaa 
      aaccaattc gattttgga tcggcttaa tgagaagtt 
      taaaaatga aggtggtaa cgaaacgaa tgagcaaaa 
      gtttgatgt gaaagggag gcattattc aaaacctgc 
      cgttagttt tgacgaaaa tatcgacag agcgagaag 
      acgcaggca aattacgat ttcgaacgc catgaagtc 
      gaattttac ggaagatga tgaggagcg gaatttagc 
      aatggaaga aggttttgt ccccgatgg gatagccga 
      ccacagagg cacagttaa tgaaacaac tctgaacga 
      ctacagtaa cagcgaatt ttggtcatc tttttcaag 
      ctctgagga ggctctttt aaattgtgc tggtttaat 
      tctcaatct tccactcac gccacatca caagtgtgt 
      cccagaatt gacggaaga gcaagccga agaaacgta 
      tcttgcaaa taccatcac ttaccaagt tcctgtgaa 
      tggttacta ctttttcgt cttcaacag tgaaaatga 
      ggtacaacc gaactacat ccgcgtcca attccacct 
      aaataaggc cgtttataa cgtttcaaa cgccgtatc 
      gacttgctt gaacagttc cgccgcttg tgcgatgga 
      cctgaagtt tttctcctc ggaaaaact ggtgatgga 
      actaccagt taaagaaaa tgggaatct ttggaatga 
      agaattcgt tgtggtatc tgagtgtga accacgaac 
      cgctattta tgcaatatg tgtgattgc tgtacctgt 
      tcttgtgat actttttgc attttcgtg atgaataaa 
      agattttta gattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY