Status
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Gene information
General information
TC031198
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009094,TC009093
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6566633..6570760
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031198
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031198 RA
      MLSVSILFY FLLTVGAQR ISGNCQTPD NEPGLCLVA 
      QSCKQMLDI LRKLPRPFP PHIRAKLEA YKCVIKGKK 
      NTICCPTNP VNYNQFITN GNSAEDDVM LPDVSNHKN 
      VKFLPKNCG HLDTVDKIV NGNKTGLFE FPWMALLSY 
      QTDRGPSFL CGGTIINEN YILTAAHCV TNIKPKLIG 
      VRVGEHDIR TNTDCEEFE GEEVCAPPV QDLSIEKVI 
      FHKQYDIVT HANDIALVR VSPINLSLE NSRPVCLPL 
      DKARNFNFT NKNVVVTGW GHTEKGVPS PELLKVEVP 
      IVSFEECRN KFEKIVQLT KKQICAGGK SKSDSCSGD 
      SGGPLHVFS LLFGEPRFV QQGIVSFGP KDCGNVPFP 
      VTVSVRDKN RVQVKCPEK HNCVPLSQC EIREADTFI 
      YPCHSGLNM ICCPHGSHN VNTTLLENR KTSLFPVTC 
      GLVMVSDKV SGGKVADLG QFPWMALLG YRQKGLNYT 
      QFLCAGSII TDHYILTAA HCINLDRRL ELVLVRLGE 
      HDLLADKDC FTINNYTTC APPHVDFTI QEVTVHKQY 
      NTRTIQNDI ALIKVRRQI RFTEYIKPI CLPFERHLE 
      LKDLAKQKL TISGWGKTN AANLGGSTT LQYTSVSVW 
      NHTACKKSV PPEVQPIQS TQICANGPA KEDACKGDS 
      GGPLFLLHQ V
    CDS sequence
    • >TC031198 RA
      atgctaagc gtttcaatt ttgttctat ttccttcta 
      actgtggga gcacaaaga attagtggc aattgccaa 
      accccggat aacgaaccg ggcttatgt ttggtggcc 
      cagtcttgc aaacaaatg ttggacatt ttgcgaaaa 
      ctccctcgt ccttttcca ccccacatc cgtgcaaaa 
      ctagaagcg tataagtgc gtcattaag ggcaagaaa 
      aatacaatt tgttgtcca acaaatcct gttaattac 
      aaccagttt ataacgaat ggtaactct gccgaagat 
      gatgttatg ttgcctgat gttagcaat cacaaaaac 
      gttaaattt ttacctaaa aactgcggt catttggac 
      actgttgac aaaatcgtc aacggtaac aaaactggg 
      cttttcgaa tttccttgg atggctcta ctctcgtat 
      caaacggac cgtgggcct tcgttccta tgtgggggt 
      accataatc aacgaaaac tatatttta acggcagct 
      cattgtgtt acaaacatt aagccaaaa ctaattgga 
      gtacgagtt ggggagcac gacattcgt accaacacc 
      gactgtgaa gagtttgaa ggtgaagaa gtttgtgct 
      cctcctgtt caggattta tcaattgag aaagttatc 
      tttcacaaa caatacgac atcgtcacg catgctaat 
      gacattgct ctcgttaga gtgtcccca ataaacctg 
      tctttagag aatagtaga ccggtttgt ttacctttg 
      gacaaagcc cgaaatttc aactttaca aacaaaaac 
      gtggtagtg actggttgg ggacacacc gaaaaaggg 
      gtccccagc cctgagtta ctgaaagtt gaagttcca 
      atagtatcc tttgaagaa tgtcgaaat aaattcgaa 
      aaaattgtt caacttacc aaaaagcaa atttgcgcc 
      gggggcaag tcaaaaagt gattcatgc agcggcgac 
      agtggaggg ccccttcat gttttctca ttattattc 
      ggagaacca agatttgtt cagcaaggg atagtgtct 
      tttggccct aaagactgt ggcaatgtt ccgtttccg 
      gttactgta tccgtgcga gacaaaaac agagtccag 
      gtgaaatgt cccgaaaaa cacaactgt gtccccctt 
      tcacaatgc gagatacga gaggccgat accttcatc 
      tacccttgc cacagcggt ctcaacatg atatgttgt 
      cctcatggc tcacacaat gttaatact acacttctg 
      gaaaaccgg aaaacatct ctatttccg gttacttgt 
      ggccttgtt atggtctca gataaggtc agtggtggc 
      aaagtagca gatttgggc cagttccct tggatggcc 
      ttgctaggc tatcgacaa aaaggactc aactatacc 
      caatttttg tgcgccggt agcattatc actgaccat 
      tacatcttg acagccgct cattgcata aatctggac 
      cgacgatta gaattagtg ctggttcga ttaggagag 
      cacgatctt ttggccgac aaagattgt ttcacgatt 
      aataactac acgacttgt gctccacct catgtagat 
      ttcaccata caagaagta acagttcac aaacaatac 
      aacactcga actattcaa aacgatatt gccttgatc 
      aaagtgaga agacagatt aggttcaca gaatacatc 
      aaaccgatt tgcttacct ttcgagaga cacttggaa 
      ctcaaagat ttagccaaa caaaaactc acgataagt 
      ggttggggt aagaccaat gctgccaat ttgggcggc 
      tctacaacc ctacaatac acttctgtg tcggtttgg 
      aaccacaca gcgtgcaag aagagcgtt cctcctgag 
      gtacaaccc atacaaagc acccaaatt tgtgccaat 
      ggaccggcg aaagaagat gcttgcaag ggggacagt 
      ggaggacct ctatttctt ttgcatcaa gtctga
    mRNA sequence
    • >TC031198 RA
      tactttggt ggtttaaga tcggctgta ttgcaatgc 
      taagcgttt caattttgt tctatttcc ttctaactg 
      tgggagcac aaagaatta gtggcaatt gccaaaccc 
      cggataacg aaccgggct tatgtttgg tggcccagt 
      cttgcaaac aaatgttgg acattttgc gaaaactcc 
      ctcgtcctt ttccacccc acatccgtg caaaactag 
      aagcgtata agtgcgtca ttaagggca agaaaaata 
      caatttgtt gtccaacaa atcctgtta attacaacc 
      agtttataa cgaatggta actctgccg aagatgatg 
      ttatgttgc ctgatgtta gcaatcaca aaaacgtta 
      aatttttac ctaaaaact gcggtcatt tggacactg 
      ttgacaaaa tcgtcaacg gtaacaaaa ctgggcttt 
      tcgaatttc cttggatgg ctctactct cgtatcaaa 
      cggaccgtg ggccttcgt tcctatgtg ggggtacca 
      taatcaacg aaaactata ttttaacgg cagctcatt 
      gtgttacaa acattaagc caaaactaa ttggagtac 
      gagttgggg agcacgaca ttcgtacca acaccgact 
      gtgaagagt ttgaaggtg aagaagttt gtgctcctc 
      ctgttcagg atttatcaa ttgagaaag ttatctttc 
      acaaacaat acgacatcg tcacgcatg ctaatgaca 
      ttgctctcg ttagagtgt ccccaataa acctgtctt 
      tagagaata gtagaccgg tttgtttac ctttggaca 
      aagcccgaa atttcaact ttacaaaca aaaacgtgg 
      tagtgactg gttggggac acaccgaaa aaggggtcc 
      ccagccctg agttactga aagttgaag ttccaatag 
      tatcctttg aagaatgtc gaaataaat tcgaaaaaa 
      ttgttcaac ttaccaaaa agcaaattt gcgccgggg 
      gcaagtcaa aaagtgatt catgcagcg gcgacagtg 
      gagggcccc ttcatgttt tctcattat tattcggag 
      aaccaagat ttgttcagc aagggatag tgtcttttg 
      gccctaaag actgtggca atgttccgt ttccggtta 
      ctgtatccg tgcgagaca aaaacagag tccaggtga 
      aatgtcccg aaaaacaca actgtgtcc ccctttcac 
      aatgcgaga tacgagagg ccgatacct tcatctacc 
      cttgccaca gcggtctca acatgatat gttgtcctc 
      atggctcac acaatgtta atactacac ttctggaaa 
      accggaaaa catctctat ttccggtta cttgtggcc 
      ttgttatgg tctcagata aggtcagtg gtggcaaag 
      tagcagatt tgggccagt tcccttgga tggccttgc 
      taggctatc gacaaaaag gactcaact atacccaat 
      ttttgtgcg ccggtagca ttatcactg accattaca 
      tcttgacag ccgctcatt gcataaatc tggaccgac 
      gattagaat tagtgctgg ttcgattag gagagcacg 
      atcttttgg ccgacaaag attgtttca cgattaata 
      actacacga cttgtgctc cacctcatg tagatttca 
      ccatacaag aagtaacag ttcacaaac aatacaaca 
      ctcgaacta ttcaaaacg atattgcct tgatcaaag 
      tgagaagac agattaggt tcacagaat acatcaaac 
      cgatttgct tacctttcg agagacact tggaactca 
      aagatttag ccaaacaaa aactcacga taagtggtt 
      ggggtaaga ccaatgctg ccaatttgg gcggctcta 
      caaccctac aatacactt ctgtgtcgg tttggaacc 
      acacagcgt gcaagaaga gcgttcctc ctgaggtac 
      aacccatac aaagcaccc aaatttgtg ccaatggac 
      cggcgaaag aagatgctt gcaaggggg acagtggag 
      gacctctat ttcttttgc atcaagtct gacttgtgg 
      ggacccaaa cctaccaac tgtgtacac gagagtaga 
      caagtactt gcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY