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Gene information
General information
TC009075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7346481..7348779
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009075
    iBeetle screen iB_04587

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009075 RA
      MSTDVPPLK PRRKKLEVP VHKTSDDSK INLETKKTY 
      SANFHSKRY KNRDEKSPR DVDSVSQKV SRRYLSKEN 
      TRLAKENEE IVVKFNELE EMSVRKILK LKEKISTLQ 
      NTNEELSKE NIQLKDYSQ DLLTTLEDL KLQFEISKH 
      CRKCEESKQ TLQKLTEEN TILRNTKKD LEEDLNMLK 
      TVVYRLNVQ MERYQEKLR KLNVKVAQN ATPQHQSLD 
      NIPVVDGHD PNIFELHKD HSHTPISWG KVNSHTLGP 
      LLDAYQDTI TEKDEIIES YEREISEFT GKLKDVIKE 
      NELLHKSLT EDNDCSSKL KIELDNVKH ELKDLRDQN 
      DILIKKCAL KQDKLEEVL KCYEYKVEQ MKRDYSVVH 
      DQYCKSRTE VAALKERNK ALLDTQDEF KNERQSYIP 
      LSVHTSSVN ECKKWYEEL KHQYEQEKV KLKENIEVQ 
      AKHIEDFEK KISQHLKEK EELETTIKH LEKQIKKGE 
      AKYIELEHT LSEVQFAKS ACRKQLNKA MSFAKDLVA 
      EQETLLKAL NQRQQENKA VKKIGCDIA TRMDTLRNQ 
      LKDVQRDAW QELSTVEKR IQDQNEAME AMKQEHSEE 
      IEKLQKIIK EQESNMLLK DSTTLPLSH YLLFRDKHK
    CDS sequence
    • >TC009075 RA
      atgtcgact gatgttccg ccgttgaaa ccacgccga 
      aaaaaatta gaagttcca gtacacaaa acaagcgat 
      gattctaaa attaatctc gaaactaaa aaaacatac 
      agtgccaat tttcattca aaaaggtac aaaaataga 
      gacgagaag tcaccaaga gatgtcgat tccgtgtcg 
      cagaaagtc agtaggaga tatctcagt aaggaaaat 
      acgcgtctt gcgaaagaa aacgaggaa attgttgtt 
      aaatttaac gaattggaa gaaatgagc gtgagaaag 
      atacttaaa cttaaagaa aaaatttca acgcttcaa 
      aacaccaac gaggaatta agcaaagag aacatccaa 
      ctcaaggac tattctcag gaccttttg acaactctt 
      gaagactta aaattgcaa ttcgaaatt agcaaacat 
      tgccgaaaa tgcgaagag tccaaacaa actcttcaa 
      aaattaacc gaagagaat acgattttg cgaaatact 
      aaaaaggat ttagaggaa gatttaaac atgctcaaa 
      actgtggtt taccgactg aacgttcaa atggaacgt 
      tatcaagaa aaattgagg aaactcaac gtcaaagtc 
      gctcaaaac gccactcct cagcaccaa agtcttgac 
      aatattcca gtggtcgat ggtcacgac cctaacatt 
      ttcgagctc cacaaagac cactctcat acacccatc 
      tcttggggg aaagtcaat tcgcacact ttgggccct 
      ctcttggac gcttatcaa gacacgata acagaaaaa 
      gacgaaatt atcgaatct tacgaaaga gaaatttct 
      gaattcacc ggaaaactt aaagacgtt attaaggaa 
      aacgagctc ttgcataaa agtctcact gaagacaac 
      gactgcagc agtaaatta aaaatagag ttagacaac 
      gttaaacac gaattgaaa gatttaaga gatcagaac 
      gatatttta ataaaaaag tgtgctcta aagcaggat 
      aagctggag gaggttttg aagtgttac gagtataaa 
      gttgagcaa atgaagaga gattactcg gttgttcat 
      gaccagtac tgtaaaagt cgaactgaa gttgctgcc 
      ctgaaagaa agaaataaa gcgttgctg gacactcaa 
      gacgagttc aaaaacgaa agacagagt tatattccc 
      ttgtcggtt cacacgtca agtgtgaac gagtgtaaa 
      aagtggtac gaagagttg aaacatcag tacgaacaa 
      gaaaaagtt aaacttaaa gaaaatatc gaagttcaa 
      gctaagcac attgaagat tttgaaaag aagatttcc 
      caacactta aaggagaaa gaggagctt gaaactacg 
      atcaaacat ttggaaaaa cagataaag aagggcgaa 
      gcaaaatac atcgaattg gaacacact ttgagcgag 
      gttcaattt gcgaaaagt gcgtgcagg aagcagctc 
      aataaagcg atgagtttc gcgaaggat ttggtggcg 
      gagcaggaa actctgctg aaagctctc aatcaaagg 
      cagcaggaa aataaagct gtgaaaaag attgggtgt 
      gatattgcc acacggatg gacactttg cgaaatcaa 
      ttgaaggac gttcaaaga gatgcgtgg caggaattg 
      agcactgtt gagaaacgc attcaggat cagaacgag 
      gcgatggag gcgatgaaa caagagcac agtgaagag 
      atcgaaaag ttgcaaaag attattaag gaacaggag 
      agcaatatg ttgctcaag gattctacg acgcttcca 
      ctatctcac tacttactt tttagggac aaacataag 
      tga
    mRNA sequence
    • >TC009075 RA
      ggtgttact tattgattt ttcttcatt gttaagtca 
      atagttggc aaacaaaat aaacgaaaa atggtcaaa 
      taaatgtct tttttcttt aatattttt acaatgtcg 
      actgatgtt ccgccgttg aaaccacgc cgaaaaaaa 
      ttagaagtt ccagtacac aaaacaagc gatgattct 
      aaaattaat ctcgaaact aaaaaaaca tacagtgcc 
      aattttcat tcaaaaagg tacaaaaat agagacgag 
      aagtcacca agagatgtc gattccgtg tcgcagaaa 
      gtcagtagg agatatctc agtaaggaa aatacgcgt 
      cttgcgaaa gaaaacgag gaaattgtt gttaaattt 
      aacgaattg gaagaaatg agcgtgaga aagatactt 
      aaacttaaa gaaaaaatt tcaacgctt caaaacacc 
      aacgaggaa ttaagcaaa gagaacatc caactcaag 
      gactattct caggacctt ttgacaact cttgaagac 
      ttaaaattg caattcgaa attagcaaa cattgccga 
      aaatgcgaa gagtccaaa caaactctt caaaaatta 
      accgaagag aatacgatt ttgcgaaat actaaaaag 
      gatttagag gaagattta aacatgctc aaaactgtg 
      gtttaccga ctgaacgtt caaatggaa cgttatcaa 
      gaaaaattg aggaaactc aacgtcaaa gtcgctcaa 
      aacgccact cctcagcac caaagtctt gacaatatt 
      ccagtggtc gatggtcac gaccctaac attttcgag 
      ctccacaaa gaccactct catacaccc atctcttgg 
      gggaaagtc aattcgcac actttgggc cctctcttg 
      gacgcttat caagacacg ataacagaa aaagacgaa 
      attatcgaa tcttacgaa agagaaatt tctgaattc 
      accggaaaa cttaaagac gttattaag gaaaacgag 
      ctcttgcat aaaagtctc actgaagac aacgactgc 
      agcagtaaa ttaaaaata gagttagac aacgttaaa 
      cacgaattg aaagattta agagatcag aacgatatt 
      ttaataaaa aagtgtgct ctaaagcag gataagctg 
      gaggaggtt ttgaagtgt tacgagtat aaagttgag 
      caaatgaag agagattac tcggttgtt catgaccag 
      tactgtaaa agtcgaact gaagttgct gccctgaaa 
      gaaagaaat aaagcgttg ctggacact caagacgag 
      ttcaaaaac gaaagacag agttatatt cccttgtcg 
      gttcacacg tcaagtgtg aacgagtgt aaaaagtgg 
      tacgaagag ttgaaacat cagtacgaa caagaaaaa 
      gttaaactt aaagaaaat atcgaagtt caagctaag 
      cacattgaa gattttgaa aagaagatt tcccaacac 
      ttaaaggag aaagaggag cttgaaact acgatcaaa 
      catttggaa aaacagata aagaagggc gaagcaaaa 
      tacatcgaa ttggaacac actttgagc gaggttcaa 
      tttgcgaaa agtgcgtgc aggaagcag ctcaataaa 
      gcgatgagt ttcgcgaag gatttggtg gcggagcag 
      gaaactctg ctgaaagct ctcaatcaa aggcagcag 
      gaaaataaa gctgtgaaa aagattggg tgtgatatt 
      gccacacgg atggacact ttgcgaaat caattgaag 
      gacgttcaa agagatgcg tggcaggaa ttgagcact 
      gttgagaaa cgcattcag gatcagaac gaggcgatg 
      gaggcgatg aaacaagag cacagtgaa gagatcgaa 
      aagttgcaa aagattatt aaggaacag gagagcaat 
      atgttgctc aaggattct acgacgctt ccactatct 
      cactactta ctttttagg gacaaacat aagtgatgt 
      attacaaat tttagtaat ttgttgggt ttttggtta 
      ataaattat aaaaatttg g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY