| General information | |||
| TC033687
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 7428188..7541434 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033687 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04585 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva segment posterior randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva segment posterior randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
Fertility
egg production: number no/almost no eggs -
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC033687 RA
MALVVQKVY FMDHVWKVI WMCVVCYQG IGAKFVEEP ASATYSVEA VQGGMAKLP CDIIPSMPG DKMHIVIWF KEGENRKTP IYSFDSRDK LPEQGKHWH DESVLGARA HFRYQDKPA ILTLDSVRD SDGGIYHCR VDFKQTPTR NIKVNLTII IPPDQLSVL DETGVHISN YILGPYNEG SSVNITCVA TGGRPPPRV TWWQENALL DDSFEYLKD RRVRNILHL EKLERKHLH TVFTCQASN NNLVAPISS SVTLDINLR PLRVKLLGE NKALSADNT YELSCEVVG SRPQPTITW WKGSVQMKN TRETTSPDL NTTTSVLTF TPTVEDGGK YLSCRGQQP YISDSGLED GWKLDIYHV PLVTLELGS TLNGSTIKE GVDVYFECN IKSNPWVYK VSWRHNGKQ LYNNAQANT IVSNQSLVL QSITRARSG HYTCVGHNQ EGDGESNSV QLDVKFIPT CRPAQPKVF GVARHETAR ILCEVEANP TDVQFIWKF NNSADTVDI PQNQIYSER TRSTAAYKP MTEGDYGTL LCWGRNEIG LQKEPCVFY INPAGKPDA LSNCTILNQ TAESLHVEC TEGFDGGLQ QEFIMEVYD TQTRKLVSN VTSKNPIFT VGGLESGLG FDIGLYASN KKGRSDVSH LHAFTLKSA EKHTVKISK PLPPENCTL LHHSRFSLR IRCQVAEPS RDNTYFLQV FDADSRVLI GTATALSPQ DIVFDNMPR RYDDLLIFV RTKRKAIMS DANIIYVPK NTIRTGAST PALLQITPL LGALIGVVA ALILVAVII VVVIRLRGG GERDDKDYD DGGLSSSGR RCVAGNGDK ASTEPLNKD LNDSVDSLE EKNPDIIPQ NNAEDDYQD EERAFERLN NAPLRVYSR MQSPNNQTK NGGYDGYKT SDEITYAEL SLTNQQMPH VIYNQQAIP MSVIRRQEP TVYAQIDMK RIPQCLQPL SPPHPSSFQ TLHHPHLPA YIPRPLREE QLMHENAIN AETPLMSPR DSSACLQPL LDVSSTRTP TMTSTQPRT VTATRF
- >TC033687 RB
MALVVQKVY FMDHVWKVI WMCVVCYQG IGAKFVEEP ASATYSVEA VQGGMAKLP CDIIPSMPG DKMHIVIWF KEGENRKTP IYSFDSRDK LPEQGKHWH DESVLGARA HFRYQDKPA ILTLDSVRD SDGGIYHCR VDFKQTPTR NIKVNLTII IPPDQLSVL DETGVHISN YILGPYNEG SSVNITCVA TGGRPPPRV TWWQENALL DDSFEYLKD RRVRNILHL EKLERKHLH TVFTCQASN NNLVAPISS SVTLDINLR PLRVKLLGE NKALSADNT YELSCEVVG SRPQPTITW WKGSVQMKN TRETTSPDL NTTTSVLTF TPTVEDGGK YLSCRGQQP YISDSGLED GWKLDIYHV PLVTLELGS TLNGSTIKE GVDVYFECN IKSNPWVYK VSWRHNGKQ LYNNAQANT IVSNQSLVL QSITRARSG HYTCVGHNQ EGDGESNSV QLDVKFIPT CRPAQPKVF GVARHETAR ILCEVEANP TDVQFIWKF NNSADTVDI PQNQIYSER TRSTAAYKP MTEGDYGTL LCWGRNEIG LQKEPCVFY INPAGKPDA LSNCTILNQ TAESLHVEC TEGFDGGLQ QEFIMEVYD TQTRKLVSN VTSKNPIFT VGGLESGLG FDIGLYASN KKGRSDVSH LHAFTLKSA EKHTVKISK PLPPENCTL LHHSRFSLR IRCQVAEPS RDNTYFLQV FDADSRVLI GTATALSPQ DIVFDNMPR RYDDLLIFV RTKRKAIMS DANIIYVPK NTIRTGAST PALLQITPL LGALIGVVA ALILVAVII VVVIRLRGG GERDDKDYD DGGLSSSGR RCVAGNGDK ASTEPLNKD LNDSVDSLE EKNPDIIPQ NNAEDDYQD EERAFERLN NAPLRVYSR MQSPNNQTK NGGYDGYKT MPHVIYNQQ AIPMSVIRR QEPTVYAQI DMKRIPQCL QPLSPPHPS SFQTLHHPH LPAYIPRPL REEQLMHEN AINAETPLM SPRDSSACL QPLLDVSST RTPTMTSTQ PRTVTATRF
- >TC033687 RC
MALVVQKVY FMDHVWKVI WMCVVCYQG IGAKFVEEP ASATYSVEA VQGGMAKLP CDIIPSMPG DKMHIVIWF KEGENRKTP IYSFDSRDK LPEQGKHWH DESVLGARA HFRYQDKPA ILTLDSVRD SDGGIYHCR VDFKQTPTR NIKVNLTII IPPDQLSVL DETGVHISN YILGPYNEG SSVNITCVA TGGRPPPRV TWWQENALL DDSFEYLKD RRVRNILHL EKLERKHLH TVFTCQASN NNLVAPISS SVTLDINLR PLRVKLLGE NKALSADNT YELSCEVVG SRPQPTITW WKGSVQMKN TRETTSPDL NTTTSVLTF TPTVEDGGK YLSCRGQQP YISDSGLED GWKLDIYHV PLVTLELGS TLNGSTIKE GVDVYFECN IKSNPWVYK VSWRHNGKQ LYNNAQANT IVSNQSLVL QSITRARSG HYTCVGHNQ EGDGESNSV QLDVKFIPT CRPAQPKVF GVARHETAR ILCEVEANP TDVQFIWKF NNSADTVDI PQNQIYSER TRSTAAYKP MTEGDYGTL LCWGRNEIG LQKEPCVFY INPAGKPDA LSNCTILNQ TAESLHVEC TEGFDGGLQ QEFIMEVYD TQTRKLVSN VTSKNPIFT VGGLESGLG FDIGLYASN KKGRSDVSH LHAFTLKSA EKHTASTPA LLQITPLLG ALIGVVAAL ILVAVIIVV VIRLRGGGE RDDKDYDDG GLSSSGRRC VAGNGDKAS TEPLNKDLN DSVDSLEEK NPDIIPQNN AEDDYQDEE RAFERLNNA PLRVYSRMQ SPNNQTKNG GYDGYKTMP HVIYNQQAI PMSVIRRQE PTVYAQIDM KRIPQCLQP LSPPHPSSF QTLHHPHLP AYIPRPLRE EQLMHENAI NAETPLMSP RDSSACLQP LLDVSSTRT PTMTSTQPR TVTATRF
- >TC033687 RA
atggctttg gtggttcaa aaagtttac tttatggac catgtgtgg aaagtgatt tggatgtgc gtggtttgc taccagggg atcggagct aaattcgtc gaagaaccc gcaagcgcc acatattcc gttgaggca gtccagggt ggcatggcg aaattacca tgcgatatt atcccatcc atgcctggt gacaagatg catatagtc atatggttt aaggagggt gagaatcgt aaaaccccc atatactcg ttcgactcc cgggataaa ctgccggag caaggaaag cactggcat gacgaaagt gtcttggga gctcgagct cacttccgt tatcaagac aaaccagct atacttaca ctggacagt gtccgagac agcgatggg ggaatctat cactgccga gtcgacttt aaacaaact cctacaagg aacattaaa gtcaacttg accattatc attccacct gatcaactg agtgtactc gacgagacg ggcgttcat atctccaac tacattcta ggaccgtat aatgagggg tcttcagtc aatattaca tgtgttgcc actggaggt cgtcccccg ccgcgggtg acatggtgg caagagaat gctctcctc gacgactct ttcgagtac ttaaaggat cgtcgagtc agaaatatc ctccacctg gaaaagctt gaaaggaag catcttcac accgtattt acttgccaa gcatccaac aacaatcta gtggccccc atttctagt tcagttact ctcgatata aatctgcga cctttaagg gtgaagctc ctcggcgaa aataaggca ttatccgct gataacact tacgaactt agttgtgaa gttgtgggt tcgaggcct cagcccact ataacttgg tggaaagga agcgtgcaa atgaaaaat accagagaa accacaagc ccggacctc aacaccact accagcgtc ttgacgttc acgccaact gtcgaagac ggaggcaaa tatctgtct tgtcgaggt caacagccg tatatctca gacagcggt ttggaagac ggatggaag ctcgatatt taccacgtt cctctcgtt acccttgag ctcggcagt acgctgaat gggagcact attaaagag ggagtcgat gtttatttc gagtgtaac atcaaatcc aatccctgg gtgtacaaa gtcagctgg aggcataac ggtaaacaa ctgtacaat aacgcgcaa gcaaataca atcgtcagc aatcagagc ttggtactc caatcgatt acgagggcc agatctgga cactacacc tgtgttggg cataatcaa gagggtgat ggtgaaagc aattcggtc caacttgac gtcaaattt attccgacc tgtcgaccc gcacaaccg aaggtcttt ggggtggca aggcacgaa acggcaagg attctatgc gaagtcgag gccaatcca acggacgtg cagtttatt tggaaattt aataactcg gccgacaca gttgacata ccacagaac cagatttac agcgagagg acgaggagc acagcggcc tacaagccc atgaccgag ggggactac gggaccttg ttgtgctgg ggcaggaac gaaataggc ttgcagaag gagccctgc gttttctac ataaacccg gcaggcaaa cctgacgct ctctccaac tgtacgatc ctcaatcag acagcagaa tcgttgcac gtggaatgt acggaaggc ttcgacggc ggtctccag caggaattc attatggaa gtgtacgac acgcagaca cgcaaattg gtgagcaac gtcacatca aaaaatccc attttcacc gtcggcgga ttggaatcc ggcttgggc ttcgacatc ggcctttac gcctctaac aagaagggc cgcagcgac gtgtcgcat ctccacgca ttcaccctc aaatcggcc gagaagcac acagttaag attagcaag ccgctgcca ccagaaaat tgcacgttg cttcaccac tctcgattc tctctcagg attaggtgt caagtggcg gaaccgagc agggacaat acgtacttc ctgcaagtg tttgacgcc gatagccgg gttttaatc gggacggcg acggcgcta agcccgcaa gatatcgtt tttgacaat atgccgaga cggtacgat gatctgttg atttttgtt aggaccaag cgaaaggcg atcatgagc gatgcgaat attatttac gtgcctaag aacacgatt agaactggt gcgtcgacc ccggcttta ctgcaaata acacccctg ctcggagct ttaatcgga gtagtggca gctttgatc ctcgttgct gtgataata gtggtggta atacgtctg cgaggtggc ggcgagagg gacgacaag gactacgac gacggcggt ttgtcatcg tcgggacga cgctgcgtt gccggaaac ggcgacaaa gccagcacg gaaccatta aataaagat ttgaacgat agtgtcgat agtttagaa gagaaaaac ccagatatt ataccgcag aataacgcc gaagacgac tatcaagat gaggaacga gcgttcgag agacttaac aacgctccc ttgagggta tacagcaga atgcaaagt cccaataat cagaccaag aatggggga tacgacggg tataaaacg agcgatgaa ataacatat gctgaattg tctctcaca aatcaacag atgccccac gtcatctac aaccagcag gccatcccc atgtcggtg atccgccgg caggagccg accgtgtac gcgcaaatc gacatgaaa cgcattccg caatgtctg cagcctctg agtccgccg catccctcg tctttccag actttgcac catccgcat cttcccgcc tacattcca cggcctctc cgggaggag cagctgatg cacgagaac gccatcaat gctgagacg cctttgatg agtccgcga gactcgagc gcctgcttg cagcccctt ctggacgtc agcagcacg cggacgccg acgatgacc tcgacgcag ccgaggaca gtgacggcg actcgcttt tga
- >TC033687 RB
atggctttg gtggttcaa aaagtttac tttatggac catgtgtgg aaagtgatt tggatgtgc gtggtttgc taccagggg atcggagct aaattcgtc gaagaaccc gcaagcgcc acatattcc gttgaggca gtccagggt ggcatggcg aaattacca tgcgatatt atcccatcc atgcctggt gacaagatg catatagtc atatggttt aaggagggt gagaatcgt aaaaccccc atatactcg ttcgactcc cgggataaa ctgccggag caaggaaag cactggcat gacgaaagt gtcttggga gctcgagct cacttccgt tatcaagac aaaccagct atacttaca ctggacagt gtccgagac agcgatggg ggaatctat cactgccga gtcgacttt aaacaaact cctacaagg aacattaaa gtcaacttg accattatc attccacct gatcaactg agtgtactc gacgagacg ggcgttcat atctccaac tacattcta ggaccgtat aatgagggg tcttcagtc aatattaca tgtgttgcc actggaggt cgtcccccg ccgcgggtg acatggtgg caagagaat gctctcctc gacgactct ttcgagtac ttaaaggat cgtcgagtc agaaatatc ctccacctg gaaaagctt gaaaggaag catcttcac accgtattt acttgccaa gcatccaac aacaatcta gtggccccc atttctagt tcagttact ctcgatata aatctgcga cctttaagg gtgaagctc ctcggcgaa aataaggca ttatccgct gataacact tacgaactt agttgtgaa gttgtgggt tcgaggcct cagcccact ataacttgg tggaaagga agcgtgcaa atgaaaaat accagagaa accacaagc ccggacctc aacaccact accagcgtc ttgacgttc acgccaact gtcgaagac ggaggcaaa tatctgtct tgtcgaggt caacagccg tatatctca gacagcggt ttggaagac ggatggaag ctcgatatt taccacgtt cctctcgtt acccttgag ctcggcagt acgctgaat gggagcact attaaagag ggagtcgat gtttatttc gagtgtaac atcaaatcc aatccctgg gtgtacaaa gtcagctgg aggcataac ggtaaacaa ctgtacaat aacgcgcaa gcaaataca atcgtcagc aatcagagc ttggtactc caatcgatt acgagggcc agatctgga cactacacc tgtgttggg cataatcaa gagggtgat ggtgaaagc aattcggtc caacttgac gtcaaattt attccgacc tgtcgaccc gcacaaccg aaggtcttt ggggtggca aggcacgaa acggcaagg attctatgc gaagtcgag gccaatcca acggacgtg cagtttatt tggaaattt aataactcg gccgacaca gttgacata ccacagaac cagatttac agcgagagg acgaggagc acagcggcc tacaagccc atgaccgag ggggactac gggaccttg ttgtgctgg ggcaggaac gaaataggc ttgcagaag gagccctgc gttttctac ataaacccg gcaggcaaa cctgacgct ctctccaac tgtacgatc ctcaatcag acagcagaa tcgttgcac gtggaatgt acggaaggc ttcgacggc ggtctccag caggaattc attatggaa gtgtacgac acgcagaca cgcaaattg gtgagcaac gtcacatca aaaaatccc attttcacc gtcggcgga ttggaatcc ggcttgggc ttcgacatc ggcctttac gcctctaac aagaagggc cgcagcgac gtgtcgcat ctccacgca ttcaccctc aaatcggcc gagaagcac acagttaag attagcaag ccgctgcca ccagaaaat tgcacgttg cttcaccac tctcgattc tctctcagg attaggtgt caagtggcg gaaccgagc agggacaat acgtacttc ctgcaagtg tttgacgcc gatagccgg gttttaatc gggacggcg acggcgcta agcccgcaa gatatcgtt tttgacaat atgccgaga cggtacgat gatctgttg atttttgtt aggaccaag cgaaaggcg atcatgagc gatgcgaat attatttac gtgcctaag aacacgatt agaactggt gcgtcgacc ccggcttta ctgcaaata acacccctg ctcggagct ttaatcgga gtagtggca gctttgatc ctcgttgct gtgataata gtggtggta atacgtctg cgaggtggc ggcgagagg gacgacaag gactacgac gacggcggt ttgtcatcg tcgggacga cgctgcgtt gccggaaac ggcgacaaa gccagcacg gaaccatta aataaagat ttgaacgat agtgtcgat agtttagaa gagaaaaac ccagatatt ataccgcag aataacgcc gaagacgac tatcaagat gaggaacga gcgttcgag agacttaac aacgctccc ttgagggta tacagcaga atgcaaagt cccaataat cagaccaag aatggggga tacgacggg tataaaacg atgccccac gtcatctac aaccagcag gccatcccc atgtcggtg atccgccgg caggagccg accgtgtac gcgcaaatc gacatgaaa cgcattccg caatgtctg cagcctctg agtccgccg catccctcg tctttccag actttgcac catccgcat cttcccgcc tacattcca cggcctctc cgggaggag cagctgatg cacgagaac gccatcaat gctgagacg cctttgatg agtccgcga gactcgagc gcctgcttg cagcccctt ctggacgtc agcagcacg cggacgccg acgatgacc tcgacgcag ccgaggaca gtgacggcg actcgcttt tga
- >TC033687 RC
atggctttg gtggttcaa aaagtttac tttatggac catgtgtgg aaagtgatt tggatgtgc gtggtttgc taccagggg atcggagct aaattcgtc gaagaaccc gcaagcgcc acatattcc gttgaggca gtccagggt ggcatggcg aaattacca tgcgatatt atcccatcc atgcctggt gacaagatg catatagtc atatggttt aaggagggt gagaatcgt aaaaccccc atatactcg ttcgactcc cgggataaa ctgccggag caaggaaag cactggcat gacgaaagt gtcttggga gctcgagct cacttccgt tatcaagac aaaccagct atacttaca ctggacagt gtccgagac agcgatggg ggaatctat cactgccga gtcgacttt aaacaaact cctacaagg aacattaaa gtcaacttg accattatc attccacct gatcaactg agtgtactc gacgagacg ggcgttcat atctccaac tacattcta ggaccgtat aatgagggg tcttcagtc aatattaca tgtgttgcc actggaggt cgtcccccg ccgcgggtg acatggtgg caagagaat gctctcctc gacgactct ttcgagtac ttaaaggat cgtcgagtc agaaatatc ctccacctg gaaaagctt gaaaggaag catcttcac accgtattt acttgccaa gcatccaac aacaatcta gtggccccc atttctagt tcagttact ctcgatata aatctgcga cctttaagg gtgaagctc ctcggcgaa aataaggca ttatccgct gataacact tacgaactt agttgtgaa gttgtgggt tcgaggcct cagcccact ataacttgg tggaaagga agcgtgcaa atgaaaaat accagagaa accacaagc ccggacctc aacaccact accagcgtc ttgacgttc acgccaact gtcgaagac ggaggcaaa tatctgtct tgtcgaggt caacagccg tatatctca gacagcggt ttggaagac ggatggaag ctcgatatt taccacgtt cctctcgtt acccttgag ctcggcagt acgctgaat gggagcact attaaagag ggagtcgat gtttatttc gagtgtaac atcaaatcc aatccctgg gtgtacaaa gtcagctgg aggcataac ggtaaacaa ctgtacaat aacgcgcaa gcaaataca atcgtcagc aatcagagc ttggtactc caatcgatt acgagggcc agatctgga cactacacc tgtgttggg cataatcaa gagggtgat ggtgaaagc aattcggtc caacttgac gtcaaattt attccgacc tgtcgaccc gcacaaccg aaggtcttt ggggtggca aggcacgaa acggcaagg attctatgc gaagtcgag gccaatcca acggacgtg cagtttatt tggaaattt aataactcg gccgacaca gttgacata ccacagaac cagatttac agcgagagg acgaggagc acagcggcc tacaagccc atgaccgag ggggactac gggaccttg ttgtgctgg ggcaggaac gaaataggc ttgcagaag gagccctgc gttttctac ataaacccg gcaggcaaa cctgacgct ctctccaac tgtacgatc ctcaatcag acagcagaa tcgttgcac gtggaatgt acggaaggc ttcgacggc ggtctccag caggaattc attatggaa gtgtacgac acgcagaca cgcaaattg gtgagcaac gtcacatca aaaaatccc attttcacc gtcggcgga ttggaatcc ggcttgggc ttcgacatc ggcctttac gcctctaac aagaagggc cgcagcgac gtgtcgcat ctccacgca ttcaccctc aaatcggcc gagaagcac acagcgtcg accccggct ttactgcaa ataacaccc ctgctcgga gctttaatc ggagtagtg gcagctttg atcctcgtt gctgtgata atagtggtg gtaatacgt ctgcgaggt ggcggcgag agggacgac aaggactac gacgacggc ggtttgtca tcgtcggga cgacgctgc gttgccgga aacggcgac aaagccagc acggaacca ttaaataaa gatttgaac gatagtgtc gatagttta gaagagaaa aacccagat attataccg cagaataac gccgaagac gactatcaa gatgaggaa cgagcgttc gagagactt aacaacgct cccttgagg gtatacagc agaatgcaa agtcccaat aatcagacc aagaatggg ggatacgac gggtataaa acgatgccc cacgtcatc tacaaccag caggccatc cccatgtcg gtgatccgc cggcaggag ccgaccgtg tacgcgcaa atcgacatg aaacgcatt ccgcaatgt ctgcagcct ctgagtccg ccgcatccc tcgtctttc cagactttg caccatccg catcttccc gcctacatt ccacggcct ctccgggag gagcagctg atgcacgag aacgccatc aatgctgag acgcctttg atgagtccg cgagactcg agcgcctgc ttgcagccc cttctggac gtcagcagc acgcggacg ccgacgatg acctcgacg cagccgagg acagtgacg gcgactcgc ttttga
- >TC033687 RA
agcgccagc agtggcgat tcgtccgct gtgttggta gcattggag gaaatccgt ttcaaagag ccgcgatct gccggtttc gtggtttga aatacacaa acagagaca gcgccgggg gtgtgaatg gtacaggtg cgagtgact atggctttg gtggttcaa aaagtttac tttatggac catgtgtgg aaagtgatt tggatgtgc gtggtttgc taccagggg atcggagct aaattcgtc gaagaaccc gcaagcgcc acatattcc gttgaggca gtccagggt ggcatggcg aaattacca tgcgatatt atcccatcc atgcctggt gacaagatg catatagtc atatggttt aaggagggt gagaatcgt aaaaccccc atatactcg ttcgactcc cgggataaa ctgccggag caaggaaag cactggcat gacgaaagt gtcttggga gctcgagct cacttccgt tatcaagac aaaccagct atacttaca ctggacagt gtccgagac agcgatggg ggaatctat cactgccga gtcgacttt aaacaaact cctacaagg aacattaaa gtcaacttg accattatc attccacct gatcaactg agtgtactc gacgagacg ggcgttcat atctccaac tacattcta ggaccgtat aatgagggg tcttcagtc aatattaca tgtgttgcc actggaggt cgtcccccg ccgcgggtg acatggtgg caagagaat gctctcctc gacgactct ttcgagtac ttaaaggat cgtcgagtc agaaatatc ctccacctg gaaaagctt gaaaggaag catcttcac accgtattt acttgccaa gcatccaac aacaatcta gtggccccc atttctagt tcagttact ctcgatata aatctgcga cctttaagg gtgaagctc ctcggcgaa aataaggca ttatccgct gataacact tacgaactt agttgtgaa gttgtgggt tcgaggcct cagcccact ataacttgg tggaaagga agcgtgcaa atgaaaaat accagagaa accacaagc ccggacctc aacaccact accagcgtc ttgacgttc acgccaact gtcgaagac ggaggcaaa tatctgtct tgtcgaggt caacagccg tatatctca gacagcggt ttggaagac ggatggaag ctcgatatt taccacgtt cctctcgtt acccttgag ctcggcagt acgctgaat gggagcact attaaagag ggagtcgat gtttatttc gagtgtaac atcaaatcc aatccctgg gtgtacaaa gtcagctgg aggcataac ggtaaacaa ctgtacaat aacgcgcaa gcaaataca atcgtcagc aatcagagc ttggtactc caatcgatt acgagggcc agatctgga cactacacc tgtgttggg cataatcaa gagggtgat ggtgaaagc aattcggtc caacttgac gtcaaattt attccgacc tgtcgaccc gcacaaccg aaggtcttt ggggtggca aggcacgaa acggcaagg attctatgc gaagtcgag gccaatcca acggacgtg cagtttatt tggaaattt aataactcg gccgacaca gttgacata ccacagaac cagatttac agcgagagg acgaggagc acagcggcc tacaagccc atgaccgag ggggactac gggaccttg ttgtgctgg ggcaggaac gaaataggc ttgcagaag gagccctgc gttttctac ataaacccg gcaggcaaa cctgacgct ctctccaac tgtacgatc ctcaatcag acagcagaa tcgttgcac gtggaatgt acggaaggc ttcgacggc ggtctccag caggaattc attatggaa gtgtacgac acgcagaca cgcaaattg gtgagcaac gtcacatca aaaaatccc attttcacc gtcggcgga ttggaatcc ggcttgggc ttcgacatc ggcctttac gcctctaac aagaagggc cgcagcgac gtgtcgcat ctccacgca ttcaccctc aaatcggcc gagaagcac acagttaag attagcaag ccgctgcca ccagaaaat tgcacgttg cttcaccac tctcgattc tctctcagg attaggtgt caagtggcg gaaccgagc agggacaat acgtacttc ctgcaagtg tttgacgcc gatagccgg gttttaatc gggacggcg acggcgcta agcccgcaa gatatcgtt tttgacaat atgccgaga cggtacgat gatctgttg atttttgtt aggaccaag cgaaaggcg atcatgagc gatgcgaat attatttac gtgcctaag aacacgatt agaactggt gcgtcgacc ccggcttta ctgcaaata acacccctg ctcggagct ttaatcgga gtagtggca gctttgatc ctcgttgct gtgataata gtggtggta atacgtctg cgaggtggc ggcgagagg gacgacaag gactacgac gacggcggt ttgtcatcg tcgggacga cgctgcgtt gccggaaac ggcgacaaa gccagcacg gaaccatta aataaagat ttgaacgat agtgtcgat agtttagaa gagaaaaac ccagatatt ataccgcag aataacgcc gaagacgac tatcaagat gaggaacga gcgttcgag agacttaac aacgctccc ttgagggta tacagcaga atgcaaagt cccaataat cagaccaag aatggggga tacgacggg tataaaacg agcgatgaa ataacatat gctgaattg tctctcaca aatcaacag atgccccac gtcatctac aaccagcag gccatcccc atgtcggtg atccgccgg caggagccg accgtgtac gcgcaaatc gacatgaaa cgcattccg caatgtctg cagcctctg agtccgccg catccctcg tctttccag actttgcac catccgcat cttcccgcc tacattcca cggcctctc cgggaggag cagctgatg cacgagaac gccatcaat gctgagacg cctttgatg agtccgcga gactcgagc gcctgcttg cagcccctt ctggacgtc agcagcacg cggacgccg acgatgacc tcgacgcag ccgaggaca gtgacggcg actcgcttt tgacctaat taatgacga ttcgggaga cttgaccta cttgtaaat caaagacta aagtcgtga gtgttacct tcactgtga gtgtgtgtg ttggaaatt ttataaacg aactttcgg aacatgctt atgaaagac ttaccggtt cgagtctac attcatttt tctattaga agaatattt tgatatttt tatagtaac ttagtaatg ttaagtaaa ggaaatgtt attattttc gaattttat tttaggttt attttgttt acaaactta gataagaaa tgcaaagac aattattgt aaagcgatg acatctgaa tccgaattt ttcgatatt attcgcctg tacataact tttatagct gtgaaaaca aatcaatta tttaaggaa aatgtatta ttataaata ttttatcta tcttgactt tttattcaa ttctagaac tgttgaaaa attcccaat atttgttgt atacaagag aattattgt accatattg tacgtacgt tgtgatttc ttttagaag aaatgtttt acgttttgt gatattttt atttagtgt attttaata ctgccataa taaatgtaa ctattttag aacctaagt atttctttg taatcaaga aatattgga tacaccgtc caatcgact taattatac ttaaaaaga gaaaacttc aacgaagtg tttgttgca ttttatact aattaaaaa agttaactg atcttttag cttagtaaa gcattaaat gtcgatttg tttcagtac aatacagtt ttatgtaaa tatgtatat aattgctca aattcgatt gttcagttt tcataaaat aaatgttga aaaatggcc aaaacatct ttctgtaaa tttaaaatc attttatta cctcatctt gtattaata tattgacaa taacaaaac aactttttt tgcttgttt tttaacttt ttggttgta ttaatctac tggacatgt aaaaatgaa atcaaagtg gccttgtcg aatttaaat acattcatt taaacggt
- >TC033687 RB
agcgccagc agtggcgat tcgtccgct gtgttggta gcattggag gaaatccgt ttcaaagag ccgcgatct gccggtttc gtggtttga aatacacaa acagagaca gcgccgggg gtgtgaatg gtacaggtg cgagtgact atggctttg gtggttcaa aaagtttac tttatggac catgtgtgg aaagtgatt tggatgtgc gtggtttgc taccagggg atcggagct aaattcgtc gaagaaccc gcaagcgcc acatattcc gttgaggca gtccagggt ggcatggcg aaattacca tgcgatatt atcccatcc atgcctggt gacaagatg catatagtc atatggttt aaggagggt gagaatcgt aaaaccccc atatactcg ttcgactcc cgggataaa ctgccggag caaggaaag cactggcat gacgaaagt gtcttggga gctcgagct cacttccgt tatcaagac aaaccagct atacttaca ctggacagt gtccgagac agcgatggg ggaatctat cactgccga gtcgacttt aaacaaact cctacaagg aacattaaa gtcaacttg accattatc attccacct gatcaactg agtgtactc gacgagacg ggcgttcat atctccaac tacattcta ggaccgtat aatgagggg tcttcagtc aatattaca tgtgttgcc actggaggt cgtcccccg ccgcgggtg acatggtgg caagagaat gctctcctc gacgactct ttcgagtac ttaaaggat cgtcgagtc agaaatatc ctccacctg gaaaagctt gaaaggaag catcttcac accgtattt acttgccaa gcatccaac aacaatcta gtggccccc atttctagt tcagttact ctcgatata aatctgcga cctttaagg gtgaagctc ctcggcgaa aataaggca ttatccgct gataacact tacgaactt agttgtgaa gttgtgggt tcgaggcct cagcccact ataacttgg tggaaagga agcgtgcaa atgaaaaat accagagaa accacaagc ccggacctc aacaccact accagcgtc ttgacgttc acgccaact gtcgaagac ggaggcaaa tatctgtct tgtcgaggt caacagccg tatatctca gacagcggt ttggaagac ggatggaag ctcgatatt taccacgtt cctctcgtt acccttgag ctcggcagt acgctgaat gggagcact attaaagag ggagtcgat gtttatttc gagtgtaac atcaaatcc aatccctgg gtgtacaaa gtcagctgg aggcataac ggtaaacaa ctgtacaat aacgcgcaa gcaaataca atcgtcagc aatcagagc ttggtactc caatcgatt acgagggcc agatctgga cactacacc tgtgttggg cataatcaa gagggtgat ggtgaaagc aattcggtc caacttgac gtcaaattt attccgacc tgtcgaccc gcacaaccg aaggtcttt ggggtggca aggcacgaa acggcaagg attctatgc gaagtcgag gccaatcca acggacgtg cagtttatt tggaaattt aataactcg gccgacaca gttgacata ccacagaac cagatttac agcgagagg acgaggagc acagcggcc tacaagccc atgaccgag ggggactac gggaccttg ttgtgctgg ggcaggaac gaaataggc ttgcagaag gagccctgc gttttctac ataaacccg gcaggcaaa cctgacgct ctctccaac tgtacgatc ctcaatcag acagcagaa tcgttgcac gtggaatgt acggaaggc ttcgacggc ggtctccag caggaattc attatggaa gtgtacgac acgcagaca cgcaaattg gtgagcaac gtcacatca aaaaatccc attttcacc gtcggcgga ttggaatcc ggcttgggc ttcgacatc ggcctttac gcctctaac aagaagggc cgcagcgac gtgtcgcat ctccacgca ttcaccctc aaatcggcc gagaagcac acagttaag attagcaag ccgctgcca ccagaaaat tgcacgttg cttcaccac tctcgattc tctctcagg attaggtgt caagtggcg gaaccgagc agggacaat acgtacttc ctgcaagtg tttgacgcc gatagccgg gttttaatc gggacggcg acggcgcta agcccgcaa gatatcgtt tttgacaat atgccgaga cggtacgat gatctgttg atttttgtt aggaccaag cgaaaggcg atcatgagc gatgcgaat attatttac gtgcctaag aacacgatt agaactggt gcgtcgacc ccggcttta ctgcaaata acacccctg ctcggagct ttaatcgga gtagtggca gctttgatc ctcgttgct gtgataata gtggtggta atacgtctg cgaggtggc ggcgagagg gacgacaag gactacgac gacggcggt ttgtcatcg tcgggacga cgctgcgtt gccggaaac ggcgacaaa gccagcacg gaaccatta aataaagat ttgaacgat agtgtcgat agtttagaa gagaaaaac ccagatatt ataccgcag aataacgcc gaagacgac tatcaagat gaggaacga gcgttcgag agacttaac aacgctccc ttgagggta tacagcaga atgcaaagt cccaataat cagaccaag aatggggga tacgacggg tataaaacg atgccccac gtcatctac aaccagcag gccatcccc atgtcggtg atccgccgg caggagccg accgtgtac gcgcaaatc gacatgaaa cgcattccg caatgtctg cagcctctg agtccgccg catccctcg tctttccag actttgcac catccgcat cttcccgcc tacattcca cggcctctc cgggaggag cagctgatg cacgagaac gccatcaat gctgagacg cctttgatg agtccgcga gactcgagc gcctgcttg cagcccctt ctggacgtc agcagcacg cggacgccg acgatgacc tcgacgcag ccgaggaca gtgacggcg actcgcttt tgacctaat taatgacga ttcgggaga cttgaccta cttgtaaat caaagacta aagtcgtga gtgttacct tcactgtga gtgtgtgtg ttggaaatt ttataaacg aactttcgg aacatgctt atgaaagac ttaccggtt cgagtctac attcatttt tctattaga agaatattt tgatatttt tatagtaac ttagtaatg ttaagtaaa ggaaatgtt attattttc gaattttat tttaggttt attttgttt acaaactta gataagaaa tgcaaagac aattattgt aaagcgatg acatctgaa tccgaattt ttcgatatt attcgcctg tacataact tttatagct gtgaaaaca aatcaatta tttaaggaa aatgtatta ttataaata ttttatcta tcttgactt tttattcaa ttctagaac tgttgaaaa attcccaat atttgttgt atacaagag aattattgt accatattg tacgtacgt tgtgatttc ttttagaag aaatgtttt acgttttgt gatattttt atttagtgt attttaata ctgccataa taaatgtaa ctattttag aacctaagt atttctttg taatcaaga aatattgga tacaccgtc caatcgact taattatac ttaaaaaga gaaaacttc aacgaagtg tttgttgca ttttatact aattaaaaa agttaactg atcttttag cttagtaaa gcattaaat gtcgatttg tttcagtac aatacagtt ttatgtaaa tatgtatat aattgctca aattcgatt gttcagttt tcataaaat aaatgttga aaaatggcc aaaacatct ttctgtaaa tttaaaatc attttatta cctcatctt gtattaata tattgacaa taacaaaac aactttttt tgcttgttt tttaacttt ttggttgta ttaatctac tggacatgt aaaaatgaa atcaaagtg gccttgtcg aatttaaat acattcatt taaacggt
- >TC033687 RC
agcgccagc agtggcgat tcgtccgct gtgttggta gcattggag gaaatccgt ttcaaagag ccgcgatct gccggtttc gtggtttga aatacacaa acagagaca gcgccgggg gtgtgaatg gtacaggtg cgagtgact atggctttg gtggttcaa aaagtttac tttatggac catgtgtgg aaagtgatt tggatgtgc gtggtttgc taccagggg atcggagct aaattcgtc gaagaaccc gcaagcgcc acatattcc gttgaggca gtccagggt ggcatggcg aaattacca tgcgatatt atcccatcc atgcctggt gacaagatg catatagtc atatggttt aaggagggt gagaatcgt aaaaccccc atatactcg ttcgactcc cgggataaa ctgccggag caaggaaag cactggcat gacgaaagt gtcttggga gctcgagct cacttccgt tatcaagac aaaccagct atacttaca ctggacagt gtccgagac agcgatggg ggaatctat cactgccga gtcgacttt aaacaaact cctacaagg aacattaaa gtcaacttg accattatc attccacct gatcaactg agtgtactc gacgagacg ggcgttcat atctccaac tacattcta ggaccgtat aatgagggg tcttcagtc aatattaca tgtgttgcc actggaggt cgtcccccg ccgcgggtg acatggtgg caagagaat gctctcctc gacgactct ttcgagtac ttaaaggat cgtcgagtc agaaatatc ctccacctg gaaaagctt gaaaggaag catcttcac accgtattt acttgccaa gcatccaac aacaatcta gtggccccc atttctagt tcagttact ctcgatata aatctgcga cctttaagg gtgaagctc ctcggcgaa aataaggca ttatccgct gataacact tacgaactt agttgtgaa gttgtgggt tcgaggcct cagcccact ataacttgg tggaaagga agcgtgcaa atgaaaaat accagagaa accacaagc ccggacctc aacaccact accagcgtc ttgacgttc acgccaact gtcgaagac ggaggcaaa tatctgtct tgtcgaggt caacagccg tatatctca gacagcggt ttggaagac ggatggaag ctcgatatt taccacgtt cctctcgtt acccttgag ctcggcagt acgctgaat gggagcact attaaagag ggagtcgat gtttatttc gagtgtaac atcaaatcc aatccctgg gtgtacaaa gtcagctgg aggcataac ggtaaacaa ctgtacaat aacgcgcaa gcaaataca atcgtcagc aatcagagc ttggtactc caatcgatt acgagggcc agatctgga cactacacc tgtgttggg cataatcaa gagggtgat ggtgaaagc aattcggtc caacttgac gtcaaattt attccgacc tgtcgaccc gcacaaccg aaggtcttt ggggtggca aggcacgaa acggcaagg attctatgc gaagtcgag gccaatcca acggacgtg cagtttatt tggaaattt aataactcg gccgacaca gttgacata ccacagaac cagatttac agcgagagg acgaggagc acagcggcc tacaagccc atgaccgag ggggactac gggaccttg ttgtgctgg ggcaggaac gaaataggc ttgcagaag gagccctgc gttttctac ataaacccg gcaggcaaa cctgacgct ctctccaac tgtacgatc ctcaatcag acagcagaa tcgttgcac gtggaatgt acggaaggc ttcgacggc ggtctccag caggaattc attatggaa gtgtacgac acgcagaca cgcaaattg gtgagcaac gtcacatca aaaaatccc attttcacc gtcggcgga ttggaatcc ggcttgggc ttcgacatc ggcctttac gcctctaac aagaagggc cgcagcgac gtgtcgcat ctccacgca ttcaccctc aaatcggcc gagaagcac acagcgtcg accccggct ttactgcaa ataacaccc ctgctcgga gctttaatc ggagtagtg gcagctttg atcctcgtt gctgtgata atagtggtg gtaatacgt ctgcgaggt ggcggcgag agggacgac aaggactac gacgacggc ggtttgtca tcgtcggga cgacgctgc gttgccgga aacggcgac aaagccagc acggaacca ttaaataaa gatttgaac gatagtgtc gatagttta gaagagaaa aacccagat attataccg cagaataac gccgaagac gactatcaa gatgaggaa cgagcgttc gagagactt aacaacgct cccttgagg gtatacagc agaatgcaa agtcccaat aatcagacc aagaatggg ggatacgac gggtataaa acgatgccc cacgtcatc tacaaccag caggccatc cccatgtcg gtgatccgc cggcaggag ccgaccgtg tacgcgcaa atcgacatg aaacgcatt ccgcaatgt ctgcagcct ctgagtccg ccgcatccc tcgtctttc cagactttg caccatccg catcttccc gcctacatt ccacggcct ctccgggag gagcagctg atgcacgag aacgccatc aatgctgag acgcctttg atgagtccg cgagactcg agcgcctgc ttgcagccc cttctggac gtcagcagc acgcggacg ccgacgatg acctcgacg cagccgagg acagtgacg gcgactcgc ttttgacct aattaatga cgattcggg agacttgac ctacttgta aatcaaaga ctaaagtcg tgagtgtta ccttcactg tgagtgtgt gtgttggaa attttataa acgaacttt cggaacatg cttatgaaa gacttaccg gttcgagtc tacattcat ttttctatt agaagaata ttttgatat ttttatagt aacttagta atgttaagt aaaggaaat gttattatt ttcgaattt tattttagg tttattttg tttacaaac ttagataag aaatgcaaa gacaattat tgtaaagcg atgacatct gaatccgaa tttttcgat attattcgc ctgtacata acttttata gctgtgaaa acaaatcaa ttatttaag gaaaatgta ttattataa atattttat ctatcttga ctttttatt caattctag aactgttga aaaattccc aatatttgt tgtatacaa gagaattat tgtaccata ttgtacgta cgttgtgat ttcttttag aagaaatgt tttacgttt tgtgatatt tttatttag tgtatttta atactgcca taataaatg taactattt tagaaccta agtatttct ttgtaatca agaaatatt ggatacacc gtccaatcg acttaatta tacttaaaa agagaaaac ttcaacgaa gtgtttgtt gcattttat actaattaa aaaagttaa ctgatcttt tagcttagt aaagcatta aatgtcgat ttgtttcag tacaataca gttttatgt aaatatgta tataattgc tcaaattcg attgttcag ttttcataa aataaatgt tgaaaaatg gccaaaaca tctttctgt aaatttaaa atcatttta ttacctcat cttgtatta atatattga caataacaa aacaacttt ttttgcttg ttttttaac tttttggtt gtattaatc tactggaca tgtaaaaat gaaatcaaa gtggccttg tcgaattta aatacattc atttaaacg gt
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