Status
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Gene information
General information
TC009058 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7588671..7727214
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009058
    iBeetle screen iB_04584

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009058 RA
      MVAVLRKLV LFFIGILPA VTAQGKFIN HFGGQVGDY 
      QTNRGTVTH VQAIVKGNA QLPCDLLPP ALNDSVVLV 
      VWYKDEHTP IYSYDTRGP YKTTPKHWQ DSNLGQRAF 
      FRTMTDPAT LNIDNINEK DEGEYRCRI DYLRSPTKN 
      LRVKLAVIV PPQKPTIFD ERGKEVPSL AGPYEEGGD 
      MKLTCFVTG GKPEPTIKW WRGEKLIES TETRSGFQN 
      VRSNQLVIK GLRRADQHA AFTCQASNN NISQPVSAT 
      TSIEIYFRP LHVEIMSSN NPYSADRLY EIACQTFGS 
      RPPAKITWY MDNKELLPP KYNYTQENS PDGNVTLSN 
      LSVILTRQD NGRTLTCKA SNDQVQNGV EEATVKLNV 
      FYVPILHLS LGSNLNPDD IEEGDDVYF ECKVNANPE 
      AYKVLWKHN DQVIQHNQK GVIVSRGNL ALQGVTRSQ 
      AGNYSCVAS NVEGDGDSN RVELKIMYK PVCRPEQKR 
      IYGVARHEN AKILCEVES YPPPDSFKW SFNNSAENV 
      DVPQMRYHS GVHHFTSTL TYTPMNELD YGTVMCWAN 
      NLAGRQLEP CIFHVIAAG KPDPPFNCT IMNQTSESL 
      DVECSEGFD GGQPQFFLL EVYDQQTDA LQANVSAKF 
      PLFTVSGLD PGKILKMVV YAANSKGKS ESVMLEGLM 
      LKAAEKQTV LSLGTRDQL EIAPILGIL VAIVTALLL 
      VTVVILGAL KVRAAHREG SRALRPGFL PVKEKVTLP 
      LRSESEDLF EKDDKNPDV IPANKDSDY QLGSAAQTP 
      GLNNSVTAT TEYETSPPQ QNLTPLAEA FMARDRAFA 
      SQHGNEVTY AELSLARPN SLETMKNGG SQYGTLRNR 
      EDSTIYAQI DHGRRLPPP IPGKTSPLV SPVSSLFPS 
      AKQGLYHRD AVTVRTPLM GCQQESCV
    CDS sequence
    • >TC009058 RA
      atggttgcg gttttgcga aaacttgta ctttttttc 
      atcgggatt ttgccagcg gtgacagca caagggaaa 
      ttcatcaat cacttcggc ggccaagtg ggtgattac 
      cagacgaac cgtggcaca gtgacgcac gtccaggcc 
      atcgtcaaa ggcaacgcg cagttgcct tgtgatctc 
      cttcctccg gcgctcaac gacagtgtc gtccttgtc 
      gtgtggtac aaagacgag cacacgcca atttacagt 
      tacgacacg agagggccg tacaaaacg acgccgaag 
      cattggcag gatagcaat ttgggccag cgagctttt 
      ttcaggaca atgacagat cctgccacg ttgaacata 
      gacaacatc aatgaaaaa gacgagggg gagtatcga 
      tgtcgcatc gattattta cgatcgccg acgaagaac 
      ttgagagta aagctggca gttatagtt cctccgcaa 
      aaaccgacc attttcgac gaacgaggt aaagaggtt 
      ccatctctg gcaggtccc tacgaagaa ggaggagac 
      atgaaatta acttgtttt gttactggg ggcaaaccg 
      gagccgacg ataaaatgg tggcgtggt gagaagctt 
      atagaatcg acggagacc cgcagtgga tttcagaac 
      gtgcgctcc aaccaactc gtcatcaaa ggcttgcga 
      agggccgat cagcacgcc gcttttaca tgccaagct 
      tccaacaac aacatcagt caaccggtg tcggccaca 
      acgtccatc gaaatatat tttcggcca ctacatgtg 
      gagataatg agcagcaac aatccctat tctgccgac 
      aggctgtac gaaatagcg tgccaaaca ttcggatca 
      aggcctccg gctaaaatt acgtggtat atggataac 
      aaggagctg cttccgcca aaatataat tacactcaa 
      gagaactcg ccggacggc aacgtgact ctgtccaat 
      ttgtctgtc atcctgacg cggcaggac aacggcagg 
      actctcacg tgcaaggct tcaaatgac caggtgcaa 
      aacggcgtc gaggaagcc accgtcaaa ctcaacgtc 
      ttctatgtg ccgatcctg cacctgtct ttgggctcg 
      aacctgaat ccggacgac atcgaagaa ggagatgat 
      gtttacttt gaatgcaaa gtgaatgct aaccctgag 
      gcatataaa gtactctgg aagcacaac gatcaagtt 
      atacaacat aatcaaaag ggcgttatt gtaagtaga 
      gggaacctg gctttacaa ggagttaca aggagccaa 
      gctggaaat tattcatgc gtagcctcc aacgtcgaa 
      ggggatggt gatagtaac agggtggaa ctcaaaatc 
      atgtacaag cccgtatgc cgtcccgaa caaaagcgc 
      atttacggc gtggcccgc cacgagaat gccaaaatc 
      ctgtgcgag gtggagtct tatccgccc cccgacagt 
      ttcaaatgg tctttcaat aactccgcg gagaacgtg 
      gacgtgccc caaatgcgc taccattcg ggcgtgcac 
      cacttcacg tccactctg acatacacc cccatgaac 
      gagctggac tacggcact gtcatgtgc tgggccaac 
      aacttggcc gggaggcag ctggagccg tgcatcttt 
      cacgtcatc gccgccggc aaaccggac cctcccttc 
      aactgcacc atcatgaac cagaccagc gaatcgctg 
      gacgtcgag tgctcggag gggttcgat gggggccaa 
      ccgcagttt tttctgctg gaagtttac gaccagcag 
      accgacgcg ctgcaggcc aatgtgtcc gctaagttc 
      cctttgttt accgtcagc ggactcgac ccgggaaag 
      atactcaaa atggtggtc tacgccgcc aactccaag 
      ggaaagagc gagtccgtc atgctggag ggcctgatg 
      ctcaaagcc gccgaaaag caaacagtt ttgtcgctg 
      ggaacgcgg gatcagctg gaaatcgcg ccaattctg 
      ggaatatta gtggccatt gtgaccgca ctcctcctg 
      gtgacggtc gtaattctg ggggctctc aaagtgcga 
      gctgcgcat cgcgagggc tctcgagct cttagacct 
      ggcttcctg cccgtcaag gagaaagtc acattgccg 
      ttacggtcc gaatccgag gatttgttt gagaaagac 
      gataaaaat ccagatgtt attccggct aataaagac 
      tccgattat caactgggg tcagccgct caaactccc 
      ggtttgaat aattcagtg acagctaca actgagtac 
      gaaacatca ccccctcag cagaatctc acgcccctc 
      gcagaagcc ttcatggct agagatcga gctttcgcc 
      tcccaacat ggcaacgag gtgacttat gcagagctg 
      tctctggca cgtccgaac tctctggag acgatgaaa 
      aacggcggt agccaatac ggcactctt cgtaatcgc 
      gaagattcg acgatatac gcccagata gaccacggc 
      agacgactg ccgccccca attcccggc aaaacttcg 
      cccctggtg tctcccgtg tcttctctc tttccttcc 
      gccaaacag gggctctac caccgggac gccgtgacg 
      gttcgaacg cctctcatg ggctgccag caggaaagt 
      tgtgtctaa
    mRNA sequence
    • >TC009058 RA
      atcaactta ttccggaaa aattccggt gagtctgga 
      cccgcccac ctccagtga catatcatc catgtgtac 
      ggagtttac tccaggatt tgcataaat tccgtgttc 
      ctggattgt tatttacat atctgacaa tgttccggc 
      gcacggcaa ccggatttt aattgcgga taagtttcc 
      cggagcgct ccagagacg ttcgctccg gaacacgat 
      aaactcgcc gaaacttga gtgattaat cttatcgat 
      tgtgggtgg ttttctgag gttttttta attcagttt 
      gggaagtgg ttaatgagt ggttcacag atcaggtgg 
      ctgtaagca aaatggttg cggttttgc gaaaacttg 
      tactttttt tcatcggga ttttgccag cggtgacag 
      cacaaggga aattcatca atcacttcg gcggccaag 
      tgggtgatt accagacga accgtggca cagtgacgc 
      acgtccagg ccatcgtca aaggcaacg cgcagttgc 
      cttgtgatc tccttcctc cggcgctca acgacagtg 
      tcgtccttg tcgtgtggt acaaagacg agcacacgc 
      caatttaca gttacgaca cgagagggc cgtacaaaa 
      cgacgccga agcattggc aggatagca atttgggcc 
      agcgagctt ttttcagga caatgacag atcctgcca 
      cgttgaaca tagacaaca tcaatgaaa aagacgagg 
      gggagtatc gatgtcgca tcgattatt tacgatcgc 
      cgacgaaga acttgagag taaagctgg cagttatag 
      ttcctccgc aaaaaccga ccattttcg acgaacgag 
      gtaaagagg ttccatctc tggcaggtc cctacgaag 
      aaggaggag acatgaaat taacttgtt ttgttactg 
      ggggcaaac cggagccga cgataaaat ggtggcgtg 
      gtgagaagc ttatagaat cgacggaga cccgcagtg 
      gatttcaga acgtgcgct ccaaccaac tcgtcatca 
      aaggcttgc gaagggccg atcagcacg ccgctttta 
      catgccaag cttccaaca acaacatca gtcaaccgg 
      tgtcggcca caacgtcca tcgaaatat attttcggc 
      cactacatg tggagataa tgagcagca acaatccct 
      attctgccg acaggctgt acgaaatag cgtgccaaa 
      cattcggat caaggcctc cggctaaaa ttacgtggt 
      atatggata acaaggagc tgcttccgc caaaatata 
      attacactc aagagaact cgccggacg gcaacgtga 
      ctctgtcca atttgtctg tcatcctga cgcggcagg 
      acaacggca ggactctca cgtgcaagg cttcaaatg 
      accaggtgc aaaacggcg tcgaggaag ccaccgtca 
      aactcaacg tcttctatg tgccgatcc tgcacctgt 
      ctttgggct cgaacctga atccggacg acatcgaag 
      aaggagatg atgtttact ttgaatgca aagtgaatg 
      ctaaccctg aggcatata aagtactct ggaagcaca 
      acgatcaag ttatacaac ataatcaaa agggcgtta 
      ttgtaagta gagggaacc tggctttac aaggagtta 
      caaggagcc aagctggaa attattcat gcgtagcct 
      ccaacgtcg aaggggatg gtgatagta acagggtgg 
      aactcaaaa tcatgtaca agcccgtat gccgtcccg 
      aacaaaagc gcatttacg gcgtggccc gccacgaga 
      atgccaaaa tcctgtgcg aggtggagt cttatccgc 
      cccccgaca gtttcaaat ggtctttca ataactccg 
      cggagaacg tggacgtgc cccaaatgc gctaccatt 
      cgggcgtgc accacttca cgtccactc tgacataca 
      cccccatga acgagctgg actacggca ctgtcatgt 
      gctgggcca acaacttgg ccgggaggc agctggagc 
      cgtgcatct ttcacgtca tcgccgccg gcaaaccgg 
      accctccct tcaactgca ccatcatga accagacca 
      gcgaatcgc tggacgtcg agtgctcgg aggggttcg 
      atgggggcc aaccgcagt tttttctgc tggaagttt 
      acgaccagc agaccgacg cgctgcagg ccaatgtgt 
      ccgctaagt tccctttgt ttaccgtca gcggactcg 
      acccgggaa agatactca aaatggtgg tctacgccg 
      ccaactcca agggaaaga gcgagtccg tcatgctgg 
      agggcctga tgctcaaag ccgccgaaa agcaaacag 
      ttttgtcgc tgggaacgc gggatcagc tggaaatcg 
      cgccaattc tgggaatat tagtggcca ttgtgaccg 
      cactcctcc tggtgacgg tcgtaattc tgggggctc 
      tcaaagtgc gagctgcgc atcgcgagg gctctcgag 
      ctcttagac ctggcttcc tgcccgtca aggagaaag 
      tcacattgc cgttacggt ccgaatccg aggatttgt 
      ttgagaaag acgataaaa atccagatg ttattccgg 
      ctaataaag actccgatt atcaactgg ggtcagccg 
      ctcaaactc ccggtttga ataattcag tgacagcta 
      caactgagt acgaaacat caccccctc agcagaatc 
      tcacgcccc tcgcagaag ccttcatgg ctagagatc 
      gagctttcg cctcccaac atggcaacg aggtgactt 
      atgcagagc tgtctctgg cacgtccga actctctgg 
      agacgatga aaaacggcg gtagccaat acggcactc 
      ttcgtaatc gcgaagatt cgacgatat acgcccaga 
      tagaccacg gcagacgac tgccgcccc caattcccg 
      gcaaaactt cgcccctgg tgtctcccg tgtcttctc 
      tctttcctt ccgccaaac aggggctct accaccggg 
      acgccgtga cggttcgaa cgcctctca tgggctgcc 
      agcaggaaa gttgtgtct aaaccgagt gtaaatagt 
      gcgtaatcg acgaatatg acatatctg atgtacaca 
      tcatatcgg gtctacgtg tctttctaa tcacatcat 
      gtctgtgtg tatatcaat tgtatatcg taacttatg 
      gaggggttc gcggccata tcatccctt tgtaatctt 
      catttctag tcatgtacg gattaccaa acttttcta 
      aactagtga atgtatttt gtacagaga cgatttttt 
      atagactga tttcgaaac ggaggcaat gagtgagcg 
      agtggaaag tctgtaggc acaaatttg tattataat 
      gtacataca caattttaa aacattgtt atcttaatg 
      aatgtgata tcatgcgct tcggtgccg gagctgctg 
      tggtataat aatgaaatt atataaatt atatacgat 
      tcgtttaat gtttcaagt ttactatgt ctctataag 
      acgatctgt aacttatgt aaacatggt aaggatatc 
      aaaataaag tgtttattg ctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY