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Gene information
General information
TC009034 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8338215..8340838
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009034
    iBeetle screen iB_04580

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009034 RA
      MALSSRICY LRCVKNAQK RITLPHQKP VIIGRNEET 
      GITDLHVSR NHLECTADL DTSKVLVKT LGKSYSGCN 
      GYALMQNET YTLKHGDRI EVRLGFHEF DIIFESQDE 
      PVVKKPRLD FFAMNSNNS NKNFSNSGK WEEIDGRDL 
      LIFTTDNCE PQSTIAAFD LDGTLIKTK SGARFPKDP 
      NDWVLNINS IPQKLQKLH DKGHKIVIL TNQSGLSND 
      FSKVKGFKG KIEAIIAKL SVPAQVFIA TGKSIYRKP 
      APGMWNVLS QSKNGGLAI DIEKSFYVG DAAGREKNW 
      APKKNKDHS ICDRLFALN VGLKFYTPE EYFLGASSV 
      PHVMPEFDP RKVGSNDYP HFASDKQEV IIMVGTPGS 
      GKSYFCKNF LVAKGYVHV SRDKLGSWQ KCAKMLEDC 
      LQKKQSVVI DNTNPDKES RQRFIDVAK RNQVDCRCF 
      LMTTSHKQA KHNNRFREM TDKSHTPVG DLVLNSYKK 
      GFQEPEMAE GFSEIVKIP FIPKFDKAE HEKLYKMFL 
      LED
    CDS sequence
    • >TC009034 RA
      atggctctt tcttcgcga atttgttac ttaagatgc 
      gtaaaaaac gcccagaaa aggatcact ttgcctcac 
      caaaagcct gtaataatc ggccgaaat gaagaaacg 
      ggaatcacg gacttacac gtatccaga aatcatctc 
      gaatgtacc gcagacttg gacacctca aaagtcctc 
      gtaaaaacc ctcggtaag tcctattcc gggtgcaac 
      ggttatgcc ttaatgcaa aatgaaaca tatactctc 
      aaacacggc gatcgaatc gaagtcaga ctcggtttc 
      cacgaattt gatattatt tttgaatct caagacgaa 
      cccgttgtt aaaaaacca agactagat tttttcgca 
      atgaattcc aataactcc aataagaat ttctcaaat 
      tcgggcaaa tgggaggag atcgacggt cgggaccta 
      ctcattttt acgacagac aactgcgag ccccaatca 
      acaattgca gcgttcgat cttgacgga actttgatc 
      aaaaccaaa tcgggggcg cggtttccg aaagacccc 
      aacgattgg gtcttgaac atcaattca atccctcaa 
      aagctgcaa aaactccac gacaagggc cacaaaatt 
      gtcattctt accaaccag tcaggcctt agtaacgat 
      tttagtaaa gtcaaaggc ttcaaggga aaaatcgaa 
      gcaataatc gcgaaatta tcagtcccc gcacaagtt 
      ttcatcgcc actggaaag tcgatttac cgcaaacca 
      gcccccggc atgtggaac gtcctcagc cagtctaaa 
      aatgggggc ttagcgatc gacatcgag aagtctttc 
      tacgtgggg gacgccgcc ggacgcgag aaaaactgg 
      gcccccaag aagaacaaa gaccactcg atttgcgac 
      cgacttttc gccctgaat gtggggctc aagttctac 
      acccctgag gagtatttt ctcggcgct tcgtcggtg 
      ccccatgta atgcccgag tttgacccg aggaaggtt 
      ggcagcaat gactacccc catttcgcc tccgataag 
      caagaagtt atcataatg gtggggacc ccagggtcg 
      gggaaaagc tacttttgt aagaacttt ctcgtggca 
      aaggggtac gtccacgtc agtcgggat aaactgggg 
      tcgtggcag aagtgtgcc aaaatgttg gaggattgt 
      ctccagaag aagcaaagt gtggtgatt gataacacg 
      aatcctgat aaggagtca aggcaaagg ttcatcgat 
      gtggcgaaa cggaatcaa gtcgattgt aggtgtttc 
      cttatgacg acgagtcac aaacaggcc aagcacaat 
      aacaggttt agggaaatg acggacaag agtcacact 
      ccagtgggg gatttggtc ctcaactcg tataaaaag 
      ggctttcag gaaccggaa atggccgag gggttctcg 
      gaaattgtc aaaattccg ttcattccg aaatttgat 
      aaggctgag catgaaaag ttgtataag atgtttttg 
      ttagaggac taa
    mRNA sequence
    • >TC009034 RA
      ccactttga aaatcatta aagttgcct ttttcgact 
      cagaaaacg atagttatc tcacttttt acagtctaa 
      attatttcg taatgtttg tgtaaggta atgcataca 
      atcctacat aacctctca tttgcgtaa aatgtcatg 
      gctctttct tcgcgaatt tgttactta agatgcgta 
      aaaaacgcc cagaaaagg atcactttg cctcaccaa 
      aagcctgta ataatcggc cgaaatgaa gaaacggga 
      atcacggac ttacacgta tccagaaat catctcgaa 
      tgtaccgca gacttggac acctcaaaa gtcctcgta 
      aaaaccctc ggtaagtcc tattccggg tgcaacggt 
      tatgcctta atgcaaaat gaaacatat actctcaaa 
      cacggcgat cgaatcgaa gtcagactc ggtttccac 
      gaatttgat attattttt gaatctcaa gacgaaccc 
      gttgttaaa aaaccaaga ctagatttt ttcgcaatg 
      aattccaat aactccaat aagaatttc tcaaattcg 
      ggcaaatgg gaggagatc gacggtcgg gacctactc 
      atttttacg acagacaac tgcgagccc caatcaaca 
      attgcagcg ttcgatctt gacggaact ttgatcaaa 
      accaaatcg ggggcgcgg tttccgaaa gaccccaac 
      gattgggtc ttgaacatc aattcaatc cctcaaaag 
      ctgcaaaaa ctccacgac aagggccac aaaattgtc 
      attcttacc aaccagtca ggccttagt aacgatttt 
      agtaaagtc aaaggcttc aagggaaaa atcgaagca 
      ataatcgcg aaattatca gtccccgca caagttttc 
      atcgccact ggaaagtcg atttaccgc aaaccagcc 
      cccggcatg tggaacgtc ctcagccag tctaaaaat 
      gggggctta gcgatcgac atcgagaag tctttctac 
      gtgggggac gccgccgga cgcgagaaa aactgggcc 
      cccaagaag aacaaagac cactcgatt tgcgaccga 
      cttttcgcc ctgaatgtg gggctcaag ttctacacc 
      cctgaggag tattttctc ggcgcttcg tcggtgccc 
      catgtaatg cccgagttt gacccgagg aaggttggc 
      agcaatgac tacccccat ttcgcctcc gataagcaa 
      gaagttatc ataatggtg gggacccca gggtcgggg 
      aaaagctac ttttgtaag aactttctc gtggcaaag 
      gggtacgtc cacgtcagt cgggataaa ctggggtcg 
      tggcagaag tgtgccaaa atgttggag gattgtctc 
      cagaagaag caaagtgtg gtgattgat aacacgaat 
      cctgataag gagtcaagg caaaggttc atcgatgtg 
      gcgaaacgg aatcaagtc gattgtagg tgtttcctt 
      atgacgacg agtcacaaa caggccaag cacaataac 
      aggtttagg gaaatgacg gacaagagt cacactcca 
      gtgggggat ttggtcctc aactcgtat aaaaagggc 
      tttcaggaa ccggaaatg gccgagggg ttctcggaa 
      attgtcaaa attccgttc attccgaaa tttgataag 
      gctgagcat gaaaagttg tataagatg tttttgtta 
      gaggactaa taacacaat tttgtgtca catgtttaa 
      aagaaatgc tcaagtcta atctcagtt tttcattat 
      acctgatgc cacagctga tacctttct cttcaagac 
      agtatacag ggtgattca aaagtctga gaccatctc 
      ttaggaagt gataaaggg catcaaact gagttaaaa 
      gcttttatg tcaaaaatt gtttgagcc ttttttcgc 
      gagatactt atagctctt caaagttgg atttttaat 
      ttgagttct tggtacttt agatcattt ttttaaatt 
      tatttagca tatacgaca tcatacaac atctgttaa 
      aacaccaat tagctgttc cgaacattt aataaactc 
      gtaaattag ccaataaat caaatttga catcattgg 
      aaacaaaag aagaacaaa taaaaacat aatattaaa 
      aattaaaaa ataaattgc ttgggaaaa aatctgagt 
      taattttta acgtttact aaacgtccc ttattacca 
      taggacaat cggttcgac acttttgaa tcgccccgt 
      ataaaagaa aatatatca tcgacgttt ttcgaaaaa 
      aaattaaga caacattaa attgataaa atagcagtt 
      ttgtaaatt gaacggcaa aaaacaaaa aatgttttt 
      agtaaaaca agaacagct tgcaaatgt tttgtttta 
      gtaacaatt tagacatta attgtgaga aaaagacat 
      cgaacattt taagaaaga ttttcttca ctcgaaata 
      cctaaacat aactctcaa aattcatgt ggtatttaa 
      taatgtatt acaatttta ttaatatgt gaattcacg 
      tttaggaaa atttaacgt cctggtttc taagtctgt 
      agatgccga ttaaaaaaa cttcgagag tataaacta 
      ataaactaa tcactgtgt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY