Status
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Gene information
General information
TC009031 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8462210..8464528
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009031
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009031 RA
      MLRNVLICI AALMYIFGC KTTKAASIL RPSKSFLEE 
      VADFDNIVE PENIQSGNN LETRESGDE EYYDEDCPN 
      GKCPRDADT STAKSILNK LGSSFLHKF FARDTSRAE 
      DYMRRQLEQ EQEKEQRQY DIDPKNDQD FMQEQERYE 
      NITNATKQL YNKLEEEQV QLGPLGEEN ICCEEEEPI 
      RRTFVQIPQ KSGIRASNG LLEMHFYEK IPAADSVKI 
      VPHEQPVFI NLYPKHEKN RRMESAKKY DYFALVPQD 
      NKLIQVPAV KFTILPEFK RRKKDIYSP YLAKLKRLH 
      PFRHGSNYR HNAHSFSLV DNKMNDDEL GSAKLYKRF 
      ANLENSNNK YLEEADFLI HNRGISLKS SLDSSSKEK 
      NEEGFNVNQ SPVAAAGAK NEAEGGQSL VNTLEGEQR 
      LVDNDNGLS LVDVEGGGQ GDSLVGSIE GGGNNLVKS 
      EVSGLGGGQ SMVKNEVNG VEEGQGLVK SEVSGLEGG 
      QSMVKNEVS GMGEGQGLV KSEVSGVEQ GQGLVKSEV 
      SGMEGGQGI DDSLVKSEV GDDGEFL
    CDS sequence
    • >TC009031 RA
      atgcttcgt aatgtttta atctgtatc gccgctttg 
      atgtatatt ttcgggtgt aaaaccaca aaggcagca 
      agcatccta agaccgagt aaaagtttc cttgaagaa 
      gtcgccgat tttgataac atagtcgag cctgaaaac 
      atccagtct ggaaacaat cttgaaacg agggaaagc 
      ggggacgag gaatactac gatgaagac tgtccgaac 
      ggaaaatgt ccccgcgat gccgacaca agcacagct 
      aaatcaatt ttaaacaaa ctgggatcc tcgtttctt 
      cacaaattt tttgcaaga gacaccagt cgcgccgag 
      gattatatg cgcagacaa ctagagcag gagcaagaa 
      aaagaacag aggcagtac gacattgat ccgaagaat 
      gatcaagat tttatgcaa gaacaggag cgatacgaa 
      aacataacg aacgctaca aaacagctc tacaacaaa 
      cttgaagaa gagcaagtg cagctgggg ccactgggg 
      gaagaaaat atttgttgt gaggaagaa gagccgatt 
      agaagaacc ttcgtacaa atcccccaa aaaagtggg 
      atacgtgcc tctaatggt ttattagaa atgcatttt 
      tacgaaaaa ataccggct gccgatagt gttaaaata 
      gtaccgcac gaacaacca gtctttatt aatttatac 
      ccaaaacat gagaaaaat cgtcggatg gaatctgcc 
      aaaaaatac gattatttt gctctcgtg ccccaagat 
      aacaagtta atacaagtt cctgccgtc aagtttact 
      atacttcca gaattcaag cgtcgaaaa aaagacatt 
      tattcgcct tatttagca aaattgaaa agactgcac 
      ccatttaga cacggctcc aactaccga cataacgca 
      cactctttc tcactagtt gataataaa atgaatgat 
      gacgagctt ggatctgca aaattatac aaaagattc 
      gccaatttg gaaaattca aataataaa tatttggaa 
      gaagccgat ttcctgata cataatcgt ggcatttca 
      ttgaagtct tctctggac agttctagt aaagaaaag 
      aatgaagaa ggatttaat gttaatcaa agtccagtt 
      gctgcagca ggagccaaa aatgaggcg gagggaggg 
      caaagtttg gtcaatacg ttggagggg gagcaacgt 
      ttagtggac aatgacaat gggctgagt ttggtagat 
      gtggagggg ggaggtcag ggcgacagc ctggttggt 
      tcaattgaa ggtgggggg aataatttg gtcaagagc 
      gaggttagc ggtttgggg ggagggcaa agtatggtc 
      aaaaatgag gttaacggt gtggaggag ggacaaggt 
      ttggtcaag agcgaggtt agcggtttg gagggaggg 
      caaagtatg gtcaaaaat gaggttagc ggtatgggg 
      gaggggcaa ggtttggtc aagagtgag gttagcggt 
      gtggagcag gggcaaggt ttggttaaa agtgaggtt 
      agtggaatg gaaggaggg caaggcatc gacgatagc 
      ttagtcaaa agtgaggtt ggggacgat ggggaattt 
      ttataa
    mRNA sequence
    • >TC009031 RA
      gagggagaa tgttagtgc ctacagctt aggcgagca 
      ttaccatcc atggatcat tcaagacgc tctatccca 
      atttcatta cgcaggaag ctgctttac ttcccaaac 
      atattaatt tgctcgtat atataagag aaaacgcaa 
      tttgaaaat aaacgcaaa tatttttgt caagctgtg 
      agcaaaatt tgaaactgt ctgattttg acaattcgc 
      gtcactttg actgtcttt tacgttagt ttttcctaa 
      tttataatg tccattttc tataatttc tacactctc 
      agatgcttc gtaatgttt taatctgta tcgccgctt 
      tgatgtata ttttcgggt gtaaaacca caaaggcag 
      caagcatcc taagaccga gtaaaagtt tccttgaag 
      aagtcgccg attttgata acatagtcg agcctgaaa 
      acatccagt ctggaaaca atcttgaaa cgagggaaa 
      gcggggacg aggaatact acgatgaag actgtccga 
      acggaaaat gtccccgcg atgccgaca caagcacag 
      ctaaatcaa ttttaaaca aactgggat cctcgtttc 
      ttcacaaat tttttgcaa gagacacca gtcgcgccg 
      aggattata tgcgcagac aactagagc aggagcaag 
      aaaaagaac agaggcagt acgacattg atccgaaga 
      atgatcaag attttatgc aagaacagg agcgatacg 
      aaaacataa cgaacgcta caaaacagc tctacaaca 
      aacttgaag aagagcaag tgcagctgg ggccactgg 
      gggaagaaa atatttgtt gtgaggaag aagagccga 
      ttagaagaa ccttcgtac aaatccccc aaaaaagtg 
      ggatacgtg cctctaatg gtttattag aaatgcatt 
      tttacgaaa aaataccgg ctgccgata gtgttaaaa 
      tagtaccgc acgaacaac cagtcttta ttaatttat 
      acccaaaac atgagaaaa atcgtcgga tggaatctg 
      ccaaaaaat acgattatt ttgctctcg tgccccaag 
      ataacaagt taatacaag ttcctgccg tcaagttta 
      ctatacttc cagaattca agcgtcgaa aaaaagaca 
      tttattcgc cttatttag caaaattga aaagactgc 
      acccattta gacacggct ccaactacc gacataacg 
      cacactctt tctcactag ttgataata aaatgaatg 
      atgacgagc ttggatctg caaaattat acaaaagat 
      tcgccaatt tggaaaatt caaataata aatatttgg 
      aagaagccg atttcctga tacataatc gtggcattt 
      cattgaagt cttctctgg acagttcta gtaaagaaa 
      agaatgaag aaggattta atgttaatc aaagtccag 
      ttgctgcag caggagcca aaaatgagg cggagggag 
      ggcaaagtt tggtcaata cgttggagg gggagcaac 
      gtttagtgg acaatgaca atgggctga gtttggtag 
      atgtggagg ggggaggtc agggcgaca gcctggttg 
      gttcaattg aaggtgggg ggaataatt tggtcaaga 
      gcgaggtta gcggtttgg ggggagggc aaagtatgg 
      tcaaaaatg aggttaacg gtgtggagg agggacaag 
      gtttggtca agagcgagg ttagcggtt tggagggag 
      ggcaaagta tggtcaaaa atgaggtta gcggtatgg 
      gggaggggc aaggtttgg tcaagagtg aggttagcg 
      gtgtggagc aggggcaag gtttggtta aaagtgagg 
      ttagtggaa tggaaggag ggcaaggca tcgacgata 
      gcttagtca aaagtgagg ttggggacg atggggaat 
      ttttataaa tataatgtt tataataaa ttttattaa 
      agctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY