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Gene information
General information
TC009024 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8575204..8587123
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009024
    iBeetle screen iB_04577

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg number increased - Number of animals showing the phenotype: 3

    Comment: lateral oviduct always empty
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head segment randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 9

    wings blistered - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009024 RA
      MSSSNRTTS KGGKGSKTD NKTHKSDKS DTGKGSDKQ 
      QASRDNTHA KSEDPFIKD KVKKLMEMS QRSEEEVCL 
      ALHDCDYDE NAAMNMLLE NMGQGEWET RVKKKKNRQ 
      ASASKQEKT EESAGGDDW NDVPSAGGG GGAGNGNEK 
      DKPRNKSGP PRLHGRHND SRGWRGREK QENERNLEE 
      GGGGGRDFH RDRRGRPGG STRGPGGGR GRGGGRAGG 
      RYPPRGSRS NAYNKPIDT WDNSNTWDN STVAATNHT 
      ENWDDFPGT DEWTTEEYT GSLADTKVF TPSVAPPID 
      AINEASSNI GLSMDNAVI NTGQQLGQN LQAGAGQLP 
      SQSPVQIGG TLNAAQTQY LNQLTQQNS ENLKQYGSA 
      HTYHAQNSS QVYNTGANT TANQTYASN QQYVSSPSG 
      YVNSSYQTG NNNYNAAEQ QATQQPVRT KTQRARVPP 
      PSKIPASAV EMPGDLNSS IGYLDVQFG AMDFMSDTN 
      SFDSVNDNK YGSSNPSGL DNTAVTSTG NMDLNTNQN 
      STLDAYSPK SNTQSSISS ALTQSLTES IPQTSEHLT 
      NAYNTAPRS STTGSTATP SVTPSGLDI GKQSESHSY 
      SQQSTYNSY QQKTNTYNA STYSGTQTS NSYVSNQAN 
      SNYANSQAA PYNTPYQNA YSSAASYQS NTNAGAFPS 
      ISQANSYPS NNQSYQQNS SQSVYGANT GLSNSSNLG 
      NTTTSQYNS YSSASNHKL SKDSYDSTS NAVSNTQTT 
      TSSNVTSTT ALSLSQSTV SVTKSTTTL GVAPVMSTP 
      YIMGQVPYF QPPVYSYEE VQMLQQRMP HMTTPYYDM 
      SYQTPTTLA AVRDGALGN VGYSMSSDG RFTRGDNNA 
      SPVPSTLSQ QTTSLTQGH QAQPILAGT GHPYFFTAL 
      NTFPNYQFG MYTQLPAAT NAHGSSNST QYPKPATYG 
      SGYGSYDTL SQTQDYNKT GYVGNNQAQ KGGGVNATS 
      TGSSGNDLS MYGKSHAAL GKVNSYDKQ GFHSGTPPP 
      FAGTLHGQN AGLAPSGTG YGPQVYIPT MTPHQQQLS 
      TQLMHQPLH QMDVRHQGR RVDSGNNSG NRSQSSNPA 
      KGGAKQPYQ TSYWNQA
    CDS sequence
    • >TC009024 RA
      atgagttcg agcaatcga accacctct aaaggtggg 
      aaagggtca aagactgac aataaaaca cacaaatca 
      gataaatcc gacacaggg aaaggctca gacaagcag 
      caagcaagc agagataac acacatgca aaatcagaa 
      gatcctttc attaaggac aaagtgaag aagctgatg 
      gagatgtct cagaggtct gaagaggag gtttgtctt 
      gctctacat gattgcgac tatgatgaa aacgcggcc 
      atgaacatg cttttggaa aatatgggg caaggtgag 
      tgggagact agagttaag aagaaaaag aaccgccaa 
      gcgtccgcc agcaagcag gaaaagaca gaggagtct 
      gcgggcggc gacgattgg aacgacgtc ccatctgcc 
      ggcgggggc ggcggtgcc ggcaacggc aacgagaag 
      gacaaaccg cggaacaag agcggtccg cctcgtctc 
      cacggccga cataatgac agccgcggg tggcgtggc 
      cgagagaaa caagaaaac gagcgtaac ctcgaagag 
      ggcggcggt ggcggtcgt gatttccac cgcgatcgt 
      cgcggcaga cctggggga tcgacgcgt ggtcctgga 
      ggcggtcga ggaaggggc ggcggacgc gctggaggc 
      agatatccg ccgagggga agtcggagc aacgcctat 
      aacaaaccc atcgacacc tgggataac tccaacacg 
      tgggataac agcacggtg gccgcaacg aaccacacg 
      gaaaattgg gatgatttt cccggcacc gacgagtgg 
      acgacggag gagtacacc ggcagcctg gctgatacg 
      aaagtgttc acgccgagc gtagcgcca cctatcgat 
      gcgatcaac gaagcgtcg tcgaacatc ggtttgtcg 
      atggacaac gcggtcatt aataccggc caacaactc 
      ggtcaaaat cttcaagcc ggcgccggt cagttgcct 
      tcgcagagt cccgtccaa attggaggg acgttgaat 
      gccgcacag acgcagtac ctcaatcaa ctcactcag 
      caaaacagc gaaaactta aagcagtac ggcagtgct 
      catacttat cacgctcaa aattcgagt caggtatac 
      aatacggga gcgaacacg acagcgaat cagacgtac 
      gcgagcaat caacagtac gtcagttcg ccgagcggt 
      tacgtgaac agttcgtat cagacggga aataataac 
      tataatgcg gcagaacag caggcgacg caacagccg 
      gtgaggacg aagacgcag agggctagg gtgccacca 
      ccgtccaag attcctgcg agtgcggtc gagatgccg 
      ggagatctg aacagtagc atcggctac ttggacgtc 
      cagtttggt gcgatggac ttcatgtcg gacactaat 
      tcgtttgat agcgttaac gataacaaa tacggttct 
      tccaaccct tccggtttg gataatacg gccgtgacg 
      tcgactggt aacatggat ctcaataca aatcaaaat 
      tccactctc gacgcatat tcacccaaa tctaataca 
      caaagcagt attagctcg gcgttaacg cagagcctg 
      acggaaagc attccacag acaagtgaa catttaaca 
      aacgcttac aatacggct ccgagaagt tcgacaacg 
      ggcagcacg gcaacgcca tctgtcaca ccctcgggc 
      cttgacatc gggaaacaa agcgaaagt cactcgtac 
      tcgcagcag tcgacgtat aattcctac cagcagaaa 
      accaacacg tataacgcg tcgacgtat agcggcacg 
      cagacttcc aattcgtac gtttcgaat caagctaac 
      agtaattac gcgaacagt caagcggct ccctacaac 
      actccttat cagaatgct tattcgtcg gcggcttct 
      tatcagtcg aacacgaac gctggtgct ttcccatcg 
      ataagtcag gcaaattcg tatccctcg aataatcag 
      tcttatcaa cagaattcc agtcagtcc gtttatggt 
      gctaatacc ggtttgagc aacagctca aatttgggt 
      aacacaacg acgagtcag tacaacagc tacagttcg 
      gcaagcaac cacaaattg agcaaggac agttacgac 
      agcacttcg aacgctgtc agcaacact caaacgacg 
      acgtcgagc aatgtaaca tcgacgacg gcgctgtcg 
      ttgtctcag agcaccgtt tcggttacg aaatcaacg 
      acaacgtta ggtgtggcg ccggtgatg agtacgccg 
      tatatcatg ggacaagta ccgtatttc caaccgccc 
      gtttactcg tatgaagaa gtacaaatg ttgcaacag 
      aggatgcca catatgacg actccctac tatgacatg 
      agttaccag actccgacg acgttggcc gctgttaga 
      gatggcgct ttgggcaac gtaggttat tcgatgtct 
      tcagacggg aggtttacg cgaggtgac aacaacgct 
      tcgccggta ccttcgact ctctcgcag cagaccact 
      tccttgacg cagggacat caagcgcag ccgattctc 
      gctgggacc ggacatccc tattttttc accgccttg 
      aataccttc cccaattac cagttcggc atgtacacg 
      caacttccg gctgcgacg aacgcgcat ggctcgagc 
      aacagcacc cagtatccg aaaccggcg acgtacggt 
      tccggctac ggcagttat gacacgttg agtcaaacg 
      caagactac aacaagacc ggttacgtc gggaacaac 
      caggcacag aaagggggc ggtgttaac gcgacttct 
      accggttcg agtggaaat gatttgtca atgtacggg 
      aaatcgcac gcagctcta ggcaaagtt aattcgtac 
      gacaaacag ggattccat tccgggacg ccaccgccg 
      tttgcaggg actttacac ggtcagaat gcaggcttg 
      gctcccagt ggaactggt tacggacca caagtctat 
      ataccgaca atgacaccg catcagcag caactatca 
      actcagttg atgcatcag ccgttacat cagatggat 
      gtcaggcac caaggccga cgcgtggat tctggaaac 
      aatagtgga aaccggtca caatcctcc aatccggcc 
      aaagggggc gcaaaacag ccgtaccaa acctcttac 
      tggaaccaa gcctaa
    mRNA sequence
    • >TC009024 RA
      atccgccat tttcgtgat cacgtgacc agttactag 
      tgcgttgtg gttttactg aattataca gatagtttg 
      taaatagta cactgtaca tactgtaca aatcatgag 
      ttcgagcaa tcgaaccac ctctaaagg tgggaaagg 
      gtcaaagac tgacaataa aacacacaa atcagataa 
      atccgacac agggaaagg ctcagacaa gcagcaagc 
      aagcagaga taacacaca tgcaaaatc agaagatcc 
      tttcattaa ggacaaagt gaagaagct gatggagat 
      gtctcagag gtctgaaga ggaggtttg tcttgctct 
      acatgattg cgactatga tgaaaacgc ggccatgaa 
      catgctttt ggaaaatat ggggcaagg tgagtggga 
      gactagagt taagaagaa aaagaaccg ccaagcgtc 
      cgccagcaa gcaggaaaa gacagagga gtctgcggg 
      cggcgacga ttggaacga cgtcccatc tgccggcgg 
      gggcggcgg tgccggcaa cggcaacga gaaggacaa 
      accgcggaa caagagcgg tccgcctcg tctccacgg 
      ccgacataa tgacagccg cgggtggcg tggccgaga 
      gaaacaaga aaacgagcg taacctcga agagggcgg 
      cggtggcgg tcgtgattt ccaccgcga tcgtcgcgg 
      cagacctgg gggatcgac gcgtggtcc tggaggcgg 
      tcgaggaag gggcggcgg acgcgctgg aggcagata 
      tccgccgag gggaagtcg gagcaacgc ctataacaa 
      acccatcga cacctggga taactccaa cacgtggga 
      taacagcac ggtggccgc aacgaacca cacggaaaa 
      ttgggatga ttttcccgg caccgacga gtggacgac 
      ggaggagta caccggcag cctggctga tacgaaagt 
      gttcacgcc gagcgtagc gccacctat cgatgcgat 
      caacgaagc gtcgtcgaa catcggttt gtcgatgga 
      caacgcggt cattaatac cggccaaca actcggtca 
      aaatcttca agccggcgc cggtcagtt gccttcgca 
      gagtcccgt ccaaattgg agggacgtt gaatgccgc 
      acagacgca gtacctcaa tcaactcac tcagcaaaa 
      cagcgaaaa cttaaagca gtacggcag tgctcatac 
      ttatcacgc tcaaaattc gagtcaggt atacaatac 
      gggagcgaa cacgacagc gaatcagac gtacgcgag 
      caatcaaca gtacgtcag ttcgccgag cggttacgt 
      gaacagttc gtatcagac gggaaataa taactataa 
      tgcggcaga acagcaggc gacgcaaca gccggtgag 
      gacgaagac gcagagggc tagggtgcc accaccgtc 
      caagattcc tgcgagtgc ggtcgagat gccgggaga 
      tctgaacag tagcatcgg ctacttgga cgtccagtt 
      tggtgcgat ggacttcat gtcggacac taattcgtt 
      tgatagcgt taacgataa caaatacgg ttcttccaa 
      cccttccgg tttggataa tacggccgt gacgtcgac 
      tggtaacat ggatctcaa tacaaatca aaattccac 
      tctcgacgc atattcacc caaatctaa tacacaaag 
      cagtattag ctcggcgtt aacgcagag cctgacgga 
      aagcattcc acagacaag tgaacattt aacaaacgc 
      ttacaatac ggctccgag aagttcgac aacgggcag 
      cacggcaac gccatctgt cacaccctc gggccttga 
      catcgggaa acaaagcga aagtcactc gtactcgca 
      gcagtcgac gtataattc ctaccagca gaaaaccaa 
      cacgtataa cgcgtcgac gtatagcgg cacgcagac 
      ttccaattc gtacgtttc gaatcaagc taacagtaa 
      ttacgcgaa cagtcaagc ggctcccta caacactcc 
      ttatcagaa tgcttattc gtcggcggc ttcttatca 
      gtcgaacac gaacgctgg tgctttccc atcgataag 
      tcaggcaaa ttcgtatcc ctcgaataa tcagtctta 
      tcaacagaa ttccagtca gtccgttta tggtgctaa 
      taccggttt gagcaacag ctcaaattt gggtaacac 
      aacgacgag tcagtacaa cagctacag ttcggcaag 
      caaccacaa attgagcaa ggacagtta cgacagcac 
      ttcgaacgc tgtcagcaa cactcaaac gacgacgtc 
      gagcaatgt aacatcgac gacggcgct gtcgttgtc 
      tcagagcac cgtttcggt tacgaaatc aacgacaac 
      gttaggtgt ggcgccggt gatgagtac gccgtatat 
      catgggaca agtaccgta tttccaacc gcccgttta 
      ctcgtatga agaagtaca aatgttgca acagaggat 
      gccacatat gacgactcc ctactatga catgagtta 
      ccagactcc gacgacgtt ggccgctgt tagagatgg 
      cgctttggg caacgtagg ttattcgat gtcttcaga 
      cgggaggtt tacgcgagg tgacaacaa cgcttcgcc 
      ggtaccttc gactctctc gcagcagac cacttcctt 
      gacgcaggg acatcaagc gcagccgat tctcgctgg 
      gaccggaca tccctattt tttcaccgc cttgaatac 
      cttccccaa ttaccagtt cggcatgta cacgcaact 
      tccggctgc gacgaacgc gcatggctc gagcaacag 
      cacccagta tccgaaacc ggcgacgta cggttccgg 
      ctacggcag ttatgacac gttgagtca aacgcaaga 
      ctacaacaa gaccggtta cgtcgggaa caaccaggc 
      acagaaagg gggcggtgt taacgcgac ttctaccgg 
      ttcgagtgg aaatgattt gtcaatgta cgggaaatc 
      gcacgcagc tctaggcaa agttaattc gtacgacaa 
      acagggatt ccattccgg gacgccacc gccgtttgc 
      agggacttt acacggtca gaatgcagg cttggctcc 
      cagtggaac tggttacgg accacaagt ctatatacc 
      gacaatgac accgcatca gcagcaact atcaactca 
      gttgatgca tcagccgtt acatcagat ggatgtcag 
      gcaccaagg ccgacgcgt ggattctgg aaacaatag 
      tggaaaccg gtcacaatc ctccaatcc ggccaaagg 
      gggcgcaaa acagccgta ccaaacctc ttactggaa 
      ccaagccta agacggatt cggacactc attgctttg 
      ccccgcccg aaatcgttt ccaatagtt ataattagc 
      ttttaattg tattttata gagcgtatc agcgtcata 
      aattattac cctgtattt attcagtct tcggtttat 
      cggaggcgt ctttatgat ttttatgtg aaataaatg 
      acttgtgcg agatggttc tttttaaca ttatacatc 
      cacagattt gagtttatt ttggtgtgc gtagcgtgc 
      aatttaagt gcaattttt tgtattgat acgactgtg 
      gatgagttt ttcttgttt tggccagtg gttgtcgca 
      aaaaataaa cgcttatgt actttaaac tttgtaatt 
      aatttaatc tcaagtctg tattatatt ttttataat 
      ggattagtt ttgattttt ttatgttaa gtaatcgtg 
      ttttgtttc atttttcga ggcgagttg gtgagcagt 
      gacgctgaa agaatttcg aagtcaatt tttcaagtt 
      tctcggttt ttataaatg gacatggat taaaaaatg 
      taaatacag ctttagagt tacaaagga attttcgta 
      ctgatcaca catgctctg tacctagtt ctgcctgtg 
      cattgtgca gtagtttag aaaaattct tctagataa 
      tttgacgta tgatgcgca gtcgtttcg actgactcg 
      gcttgggtc tctccaatc ttcttgaaa cttgtaaga 
      cggtacgtt aaggatcgc tttgtttca aacaagaga 
      ctagtgagg cgcctgagt tttaaccta caatgttta 
      ggtagattt attaactaa attatcttg tgtatctat 
      atgctactt gtataaaat aatcactta taattatgt 
      agacactgt atatgtgta gttttgttt tttgtattt 
      gctaattac gatatggaa aatacacat ggataaacc 
      ttgacacac ctactgtgt atcgtttat tattatatt 
      ttgtattta cattttgta gattcataa aagaggtat 
      gaatgttca aaaagaata aaacttaat aaaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY