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Gene information
General information
TC009020 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8620062..8621544
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009020
    iBeetle screen iB_04576

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009020 RA
      MFVEVQRLG EEEILNRFP LGAHVLGRG PALQCNDKR 
      VSRNHATLE VTPDHVKLT SIHVNPCFL KAAESKTLT 
      ILKKDTSTQ LNDGDSFAF LAEDHWFKI NIVHEDLNE 
      ESADNGAIK SEAVVKRPH ECPEEEVPP EKKIKIEEG 
      EANEEVPPN PEINIGENE EIKMEITSE LDITDSEIK 
      VEPSTSIQD EEISTNVPE NEEQGSEEM KKEDPDGEN 
      PGTSIPKPR RERCWYAAQ CYRKNPVHR QDFTHPGDS 
      DFESDPEDN RPFCTYGAS CYRKNKQHR RDFKHPLQQ 
      APVKKKRSR KKVQRLNVA DSQEMRELD SESEDDPFV 
      VSDDSEEYE GESDDDDTN WEDSQNADE DSENVKRLV 
      KEAKKFVNK
    CDS sequence
    • >TC009020 RA
      atgtttgtg gaagttcaa agactaggc gaagaagaa 
      attttaaat agatttccc ttgggggcc catgtttta 
      gggcgcggt cctgcttta cagtgcaac gacaagcga 
      gtatcacga aatcacgca actttggaa gttacacca 
      gatcacgtt aaacttact tctatacac gttaatcct 
      tgttttctc aaagccgca gaaagcaaa actctaacc 
      attctcaaa aaagataca agcacgcaa ctgaatgat 
      ggtgacagt tttgcgttt cttgctgaa gatcactgg 
      tttaaaatc aacattgtc catgaagat ttaaatgaa 
      gaaagtgct gataatggt gcaattaaa agtgaggcg 
      gtggtcaaa cggccacat gaatgtccg gaagaggaa 
      gtaccacca gaaaaaaag atcaaaatt gaagaaggt 
      gaagctaat gaagaagtt ccaccaaat cctgagatt 
      aatattgga gaaaatgaa gaaataaaa atggaaata 
      acgagtgaa ttagatata actgactca gaaataaaa 
      gtcgaaccg tcaacttcc attcaagat gaagaaatc 
      tcgactaac gttcctgaa aatgaagaa caagggtct 
      gaagaaatg aagaaagaa gatccagat ggtgaaaat 
      cccggcact tcaatcccg aaaccacgg cgtgagcgt 
      tgctggtat gcggcccaa tgctaccga aaaaaccca 
      gttcacaga caggacttc acccatcct ggtgactca 
      gactttgag agtgaccca gaagacaac agacccttt 
      tgcacttac ggcgcctct tgttacaga aaaaataag 
      caacacaga agagacttc aaacatcca ctacaacaa 
      gccccagtg aaaaaaaaa cggtcccga aagaaggtt 
      cagcgttta aatgtggcc gatagtcag gaaatgcgc 
      gagttggat tccgagtct gaggacgat ccatttgtt 
      gttagtgat gatagcgaa gaatatgag ggagagtca 
      gatgatgat gacacaaat tgggaagac tcgcaaaat 
      gcggatgag gacagcgaa aatgttaag agactggtc 
      aaggaagcg aaaaaattt gtaaataaa taa
    mRNA sequence
    • >TC009020 RA
      ttcttttaa aaagaaaaa gtcaaattc cgcgccaat 
      tatgtgacg caaaaactt caagaataa atcttttac 
      atattcgaa ttttgcaat gtttgtgga agttcaaag 
      actaggcga agaagaaat tttaaatag atttccctt 
      gggggccca tgttttagg gcgcggtcc tgctttaca 
      gtgcaacga caagcgagt atcacgaaa tcacgcaac 
      tttggaagt tacaccaga tcacgttaa acttacttc 
      tatacacgt taatccttg ttttctcaa agccgcaga 
      aagcaaaac tctaaccat tctcaaaaa agatacaag 
      cacgcaact gaatgatgg tgacagttt tgcgtttct 
      tgctgaaga tcactggtt taaaatcaa cattgtcca 
      tgaagattt aaatgaaga aagtgctga taatggtgc 
      aattaaaag tgaggcggt ggtcaaacg gccacatga 
      atgtccgga agaggaagt accaccaga aaaaaagat 
      caaaattga agaaggtga agctaatga agaagttcc 
      accaaatcc tgagattaa tattggaga aaatgaaga 
      aataaaaat ggaaataac gagtgaatt agatataac 
      tgactcaga aataaaagt cgaaccgtc aacttccat 
      tcaagatga agaaatctc gactaacgt tcctgaaaa 
      tgaagaaca agggtctga agaaatgaa gaaagaaga 
      tccagatgg tgaaaatcc cggcacttc aatcccgaa 
      accacggcg tgagcgttg ctggtatgc ggcccaatg 
      ctaccgaaa aaacccagt tcacagaca ggacttcac 
      ccatcctgg tgactcaga ctttgagag tgacccaga 
      agacaacag acccttttg cacttacgg cgcctcttg 
      ttacagaaa aaataagca acacagaag agacttcaa 
      acatccact acaacaagc cccagtgaa aaaaaaacg 
      gtcccgaaa gaaggttca gcgtttaaa tgtggccga 
      tagtcagga aatgcgcga gttggattc cgagtctga 
      ggacgatcc atttgttgt tagtgatga tagcgaaga 
      atatgaggg agagtcaga tgatgatga cacaaattg 
      ggaagactc gcaaaatgc ggatgagga cagcgaaaa 
      tgttaagag actggtcaa ggaagcgaa aaaatttgt 
      aaataaata aatagtttc gataaaata tatatattt 
      ttttaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY