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Gene information
General information
TC009018 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8641147..8650364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009018
    iBeetle screen iB_04575

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009018 RA
      MKLRFPILV TIFFIGLDT SNSTSKNKF PDYFKFGAA 
      TASYQVEGA WNEDGKGEN IWDHITHTN PTYVENEDN 
      GDVACDSYH KYKEDVAML KDLGVDYYR FSISWSRIL 
      PYGVTGSPV NKLGIEYYR NLTQELLDN GIEPMVTLF 
      HWDTPEPLQ ELGGWPNPE MEEHYAYYA RIVFEQLGD 
      LVKIWMTFN EPKQTCLEG YGKGVKAPA FTTTGMADY 
      KCTHTLIKS HAKAYHIYD EEFKAKQGG RVGMVIDTV 
      WFEPASGSE KDAEAAERA IQFTYGWYG NPIILGNYP 
      QVMIDRIDE RSKQQGFAQ SRLPKFSEE EVDYIKGTY 
      DFVALNMYT ANYAEWKEE TDISDVGYD SDLNVHLFM 
      DDSWEESAS SWLRVVPWG LRKTVNWLS KTYNNPEIF 
      ITENGVSDR GGIDDPQRI NFYREYLSN LVDAIVEDG 
      VNVTRYTAW SLMDNFEWG SGYSEKFGL YSVDFSSPE 
      RTRTKKASA DYYSQVIST RCLVDSCEN NLCADNRKF 
      PSDFKFGVA TASYQVEGA WNADGKGEN IWDHLTHSQ 
      PHLVKDNST GDIACDAYH NSKEDLALL EDLGVDFYH 
      FSLSWARIL PTGYTDGPV NEAGVKYYA NILSELEKR 
      KIEAVITLF HWDMPQKLQ DDFGGLLND TFIDVFASY 
      AQLAFRLFG SRVKYWITF NEPFIMCQH GFENARKAP 
      AITKAPGID LYTCGHVVL KAHAKTYRI YDKLYRKTQ 
      KGRIAIALD TDWFEPASA DPKDLEAAE RFLQFQFGW 
      FAHPLVFGN YPQVMIDRI EERSKKEGF KTSRLPEFT 
      KKEIDEIKG TFDFIGLNH YTSTLAKWR EDIAIGKPE 
      SSKDLSVSV SKDSSWEGS ASSWLKVVP WGLRKIAKW 
      IKDTYKNPE IMIAENGYS DPGGILNDS RRINYYREY 
      LSNVLKAIY DDGVNITAY TAWSFMDNF EWLEGYTQK 
      FGLYSVNFS DPERPRTPK SSGEIRLIG GLIQGKLHS 
      HCTQETHTF FMSHQTPGR KTGTKNRTE IVHGALQVA 
      VEWAAEDTS SA
    CDS sequence
    • >TC009018 RA
      atgaaactg agattcccc attcttgtc acgattttc 
      ttcataggg ttggacaca tcgaactcc acttcgaag 
      aacaaattc ccagactat tttaaattc ggagccgcc 
      acagcgtcg taccaagtc gaaggagct tggaacgaa 
      gacggcaaa ggcgaaaac atctgggac cacatcact 
      cacacaaac cccacatat gtcgaaaac gaagacaat 
      ggggacgtc gcttgcgac tcgtaccac aaatacaaa 
      gaggacgtc gcgatgctt aaagactta ggcgtcgac 
      tattaccga ttttccatt tcttggtcc cgaattcta 
      ccatatgga gtcaccggt tcgcctgta aataaactg 
      ggaattgag tattacaga aatttaacc caagaactg 
      ctcgataat gggatcgaa cccatggtc acactgttc 
      cattgggac actccggaa cctttgcag gagttgggc 
      gggtggcct aacccagaa atggaggaa cattacgca 
      tactatgcc aggattgtg ttcgagcaa ttgggggat 
      ttagtcaag atttggatg actttcaat gaaccgaaa 
      cagacgtgt cttgaagga tacggcaaa ggggttaaa 
      gcgccagct tttacgacc acaggaatg gctgattat 
      aaatgcaca cacactttg attaaatcg cacgcgaag 
      gcttatcac atctatgat gaggagttt aaggccaaa 
      cagggagga agagttggg atggttatt gatacggtg 
      tggtttgag ccggccagt ggaagtgaa aaggatgca 
      gaagcggcc gaaagagcc attcagttc acgtatggt 
      tggtacggc aaccccatc atcctggga aattaccct 
      caagtcatg atcgaccga attgatgaa aggagtaaa 
      cagcaaggt tttgcccaa tcaagactt ccaaagttt 
      tcagaggaa gaagtggat tatattaaa ggaacctat 
      gatttcgtt gcactcaat atgtacact gcaaattat 
      gccgaatgg aaggaagaa acagatatt tccgatgtc 
      ggttacgac agcgatttg aatgtccat ctgtttatg 
      gatgatagt tgggaagaa tcagcttca tcatggcta 
      agggttgtg ccttggggt ttaagaaaa acagtaaat 
      tggcttagt aaaacctac aataatcct gaaatcttt 
      atcactgaa aatggcgtt tccgaccgt ggcggaatc 
      gatgatcca caaagaata aatttttat agagaatat 
      ttgagtaat ctcgttgat gcgattgtg gaagatggg 
      gtaaatgtc acaagatat acagcttgg agtttgatg 
      gataacttc gaatgggga agtggatat tcggaaaag 
      tttggtttg tacagtgtt gattttagc agccctgag 
      agaacaaga acaaagaaa gcttcagct gattattat 
      tcgcaagtt ataagcact aggtgtttg gtagactca 
      tgcgaaaat aatttgtgc gctgataac cgtaaattt 
      ccttccgac ttcaaattt ggtgtcgcc acagcctcg 
      taccaggtt gaaggcgct tggaacgct gatggcaaa 
      ggcgagaac atctgggac cacctaaca cactcccaa 
      ccacactta gtcaaagac aactccacg ggagatatc 
      gcatgcgac gcctaccac aactccaag gaagacctt 
      gccctactt gaagacctt ggtgtcgac ttttaccac 
      ttttcccta tcctgggcc agaatcctc cccacggga 
      tacaccgac ggcccagtc aacgaagca ggagtcaaa 
      tactacgca aacatcctc tcagagtta gaaaaacgc 
      aaaattgaa gcagtcatc acgctgttc cattgggat 
      atgccccaa aagctccaa gacgatttc ggaggactc 
      ctcaatgat actttcatt gacgttttt gcaagttat 
      gcacaactg gcgttcagg cttttcggg agtcgggtc 
      aagtattgg ataacgttt aacgagccg tttattatg 
      tgccaacac gggtttgaa aatgctcgt aaagctccg 
      gcaattact aaagcgcca ggcattgat ttgtacact 
      tgtgggcat gtggttttg aaagctcac gccaaaacg 
      taccggatt tatgataaa ttgtaccga aaaacacaa 
      aagggcaga attgcaatt gccttagat acggactgg 
      tttgagcct gcaagtgcc gatccgaaa gacttggaa 
      gcggccgag aggtttctt caattccag tttggttgg 
      tttgcccac cctcttgtc tttgggaat tatccccaa 
      gtgatgatt gatcgaatt gaagaacga agcaaaaag 
      gaaggtttc aaaacgtcg cgtttgcca gaatttaca 
      aaaaaggaa attgacgaa attaaagga accttcgat 
      tttatcggt ttgaatcat tacacctcc accttagct 
      aaatggaga gaagatata gcaattggt aagcccgag 
      tcatcgaaa gatctttca gtcagtgta tccaaagat 
      tcaagctgg gagggttca gcgtcaagt tggttgaaa 
      gttgttcca tgggggctt cggaaaatt gcaaaatgg 
      attaaagac acctacaag aatcccgaa ataatgatc 
      gcggagaat ggatattct gaccctggt ggtattctt 
      aatgattct cgtcgaatc aactactac cgagaatac 
      ttgagtaat gttttgaaa gcgatttac gacgatggt 
      gtcaacatt accgcatac acagcatgg agctttatg 
      gataatttc gagtggtta gagggctat actcagaag 
      tttggcctc tactctgtc aatttctcc gaccccgag 
      cgtccccgc accccaaaa tcctcgggc gaaatccgt 
      cttatcgga gggttaatc caagggaaa ttgcactca 
      cactgcaca caagaaacg cacacattt tttatgtca 
      caccaaaca ccagggaga aaaaccgga acgaagaac 
      cggaccgaa atcgtacac ggagcgctg caggttgca 
      gttgaatgg gctgctgaa gacacgagt tctgcttaa
    mRNA sequence
    • >TC009018 RA
      ataagtagt caacatgaa actgagatt ccccattct 
      tgtcacgat tttcttcat agggttgga cacatcgaa 
      ctccacttc gaagaacaa attcccaga ctattttaa 
      attcggagc cgccacagc gtcgtacca agtcgaagg 
      agcttggaa cgaagacgg caaaggcga aaacatctg 
      ggaccacat cactcacac aaaccccac atatgtcga 
      aaacgaaga caatgggga cgtcgcttg cgactcgta 
      ccacaaata caaagagga cgtcgcgat gcttaaaga 
      cttaggcgt cgactatta ccgattttc catttcttg 
      gtcccgaat tctaccata tggagtcac cggttcgcc 
      tgtaaataa actgggaat tgagtatta cagaaattt 
      aacccaaga actgctcga taatgggat cgaacccat 
      ggtcacact gttccattg ggacactcc ggaaccttt 
      gcaggagtt gggcgggtg gcctaaccc agaaatgga 
      ggaacatta cgcatacta tgccaggat tgtgttcga 
      gcaattggg ggatttagt caagatttg gatgacttt 
      caatgaacc gaaacagac gtgtcttga aggatacgg 
      caaaggggt taaagcgcc agcttttac gaccacagg 
      aatggctga ttataaatg cacacacac tttgattaa 
      atcgcacgc gaaggctta tcacatcta tgatgagga 
      gtttaaggc caaacaggg aggaagagt tgggatggt 
      tattgatac ggtgtggtt tgagccggc cagtggaag 
      tgaaaagga tgcagaagc ggccgaaag agccattca 
      gttcacgta tggttggta cggcaaccc catcatcct 
      gggaaatta ccctcaagt catgatcga ccgaattga 
      tgaaaggag taaacagca aggttttgc ccaatcaag 
      acttccaaa gttttcaga ggaagaagt ggattatat 
      taaaggaac ctatgattt cgttgcact caatatgta 
      cactgcaaa ttatgccga atggaagga agaaacaga 
      tatttccga tgtcggtta cgacagcga tttgaatgt 
      ccatctgtt tatggatga tagttggga agaatcagc 
      ttcatcatg gctaagggt tgtgccttg gggtttaag 
      aaaaacagt aaattggct tagtaaaac ctacaataa 
      tcctgaaat ctttatcac tgaaaatgg cgtttccga 
      ccgtggcgg aatcgatga tccacaaag aataaattt 
      ttatagaga atatttgag taatctcgt tgatgcgat 
      tgtggaaga tggggtaaa tgtcacaag atatacagc 
      ttggagttt gatggataa cttcgaatg gggaagtgg 
      atattcgga aaagtttgg tttgtacag tgttgattt 
      tagcagccc tgagagaac aagaacaaa gaaagcttc 
      agctgatta ttattcgca agttataag cactaggtg 
      tttggtaga ctcatgcga aaataattt gtgcgctga 
      taaccgtaa atttccttc cgacttcaa atttggtgt 
      cgccacagc ctcgtacca ggttgaagg cgcttggaa 
      cgctgatgg caaaggcga gaacatctg ggaccacct 
      aacacactc ccaaccaca cttagtcaa agacaactc 
      cacgggaga tatcgcatg cgacgccta ccacaactc 
      caaggaaga ccttgccct acttgaaga ccttggtgt 
      cgactttta ccacttttc cctatcctg ggccagaat 
      cctccccac gggatacac cgacggccc agtcaacga 
      agcaggagt caaatacta cgcaaacat cctctcaga 
      gttagaaaa acgcaaaat tgaagcagt catcacgct 
      gttccattg ggatatgcc ccaaaagct ccaagacga 
      tttcggagg actcctcaa tgatacttt cattgacgt 
      ttttgcaag ttatgcaca actggcgtt caggctttt 
      cgggagtcg ggtcaagta ttggataac gtttaacga 
      gccgtttat tatgtgcca acacgggtt tgaaaatgc 
      tcgtaaagc tccggcaat tactaaagc gccaggcat 
      tgatttgta cacttgtgg gcatgtggt tttgaaagc 
      tcacgccaa aacgtaccg gatttatga taaattgta 
      ccgaaaaac acaaaaggg cagaattgc aattgcctt 
      agatacgga ctggtttga gcctgcaag tgccgatcc 
      gaaagactt ggaagcggc cgagaggtt tcttcaatt 
      ccagtttgg ttggtttgc ccaccctct tgtctttgg 
      gaattatcc ccaagtgat gattgatcg aattgaaga 
      acgaagcaa aaaggaagg tttcaaaac gtcgcgttt 
      gccagaatt tacaaaaaa ggaaattga cgaaattaa 
      aggaacctt cgattttat cggtttgaa tcattacac 
      ctccacctt agctaaatg gagagaaga tatagcaat 
      tggtaagcc cgagtcatc gaaagatct ttcagtcag 
      tgtatccaa agattcaag ctgggaggg ttcagcgtc 
      aagttggtt gaaagttgt tccatgggg gcttcggaa 
      aattgcaaa atggattaa agacaccta caagaatcc 
      cgaaataat gatcgcgga gaatggata ttctgaccc 
      tggtggtat tcttaatga ttctcgtcg aatcaacta 
      ctaccgaga atacttgag taatgtttt gaaagcgat 
      ttacgacga tggtgtcaa cattaccgc atacacagc 
      atggagctt tatggataa tttcgagtg gttagaggg 
      ctatactca gaagtttgg cctctactc tgtcaattt 
      ctccgaccc cgagcgtcc ccgcacccc aaaatcctc 
      gggcgaaat ccgtcttat cggagggtt aatccaagg 
      gaaattgca ctcacactg cacacaaga aacgcacac 
      attttttat gtcacacca aacaccagg gagaaaaac 
      cggaacgaa gaaccggac cgaaatcgt acacggagc 
      gctgcaggt tgcagttga atgggctgc tgaagacac 
      gagttctgc ttaaagatc tcgaaggca ggtggctcg 
      aaacgggtg ccgatggac aggtcgccg actgtacgg 
      tgggttgca tacaagttt ttcggtttg gtttttgag 
      aaaaaaccc aaattttgc tcttaatgt actagcttg 
      cacttctgg ataatgaca aaaatgaaa actatttat 
      aaagagagg tggtttttt aaccgcaac aatttgact 
      tgcaaagga tcatggatt tttatgggt tgtggtatt 
      gctgctggc acaatttat tttaatttt tatttaaaa 
      aagacctta tattaatcc aaaatgttt tggttaatt 
      agtacttaa ctattttta ttatttagt cttttgact 
      gctttaatt aactgagca ttcacatta aattattga 
      atgaatc
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