Status
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Gene information
General information
TC033705
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009016
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8658004..8665524
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033705
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033705 RA
      MIKVKVSIA MHPTHAKNL LFRLCVFGT LISLAKTQW 
      TFPDNFKFG VATSAYQIE GGWDADGKG VSTWDRLTH 
      NTPGMIQDG SNGDIACDS YHKWERDVE MVKETGVDY 
      YRFSLSWTR IFPQGYINL VNQPGVDYY NNLINKLIE 
      NGIEPVITL YHWDLPQMF SPLGSWASP VMVDLFGNY 
      ARKAFQLFG DRVKTWITF NEPKIVCQD FHDFLGNVT 
      SPYPKGIIE YLCTHNLLK AHAEAYHIY DKEFRPTQK 
      GRISITLNF EWAEPASDN IEDIEAAEQ RRQFEFGLY 
      ANPIINFDY PKIVKDRIA ERSRKEGYP KSRLPEFTL 
      AEKLKLRGT YDYLGLNHY STWYVKAVE DQPIGTPSY 
      PADMGTERY QDPTWEGSG ADWNKVVPW GLRHILQWI 
      KKTYRNPIV LITECGYPD RTGTVEDEP RIDFFRKYL 
      NATLEAIYE DGANVQAFM AWSLMDNFE WQQGYQLST 
      AKWKFPDDF KFGVATSAY QTEGGWNAD GRGTSIWDV 
      AAHNRSFLI RDRSNGDIA CDSYNKWEE DVLMVKNLG 
      VHYYRFSLS WTRILPDGY SSKVNPEGV KYYNKIIDK 
      LIENQIEPV VTLYHWDLP QAFSALGGW TNPIVIPHF 
      ANFARKAFR LFGDRVKIW ITINEPRLM CDFFKGLVG 
      DITPDYPLG ISEYLCAHN VLKAHAAAY HIYDKEFRP 
      IQHGKISIT LDMVWSEPA NASDPEDVR AAEQNVQFD 
      FGLYANPIF NYDYPKVVI ERVANRSKI ERIPKSRLP 
      KFTMAEKLK ISGTYDFLG INHYITMYT KATKEPPVT 
      KPSFDVDSG GKRFFDPKW EGCAADWIK VVPWGYRNS 
      LKWIKKNYR NPEIIITEN GYADGGDVI NDDGRINYY 
      REYLNATLQ AIIEDGVNV KSYIAWSLM DNYEWGFGY 
      TFSTAIEWK FPADFKFGV ATAAYQIEG GWNADGKGT 
      SIWDTLGHN RSILVKDRS NGDIACDSY HKWEEDVQM 
      VKNLGVDTY RFSVAWTRI LPEGYATRV NQPGVDYYN 
      KLINKLIEN GIEPVVTLY HWDLPQVFS PLGGWTNPV 
      VVPHFANYA RKAFELFGD RVKTWITIN EPRLICNEY 
      KGLVGDITE DFPLGVSEY LCAKNVLKA HAEAYHIYD 
      KEFRKTQKG KISITLDMT WSEPATNNP EDVKAAEQN 
      RQFDFGIYT NPIFNYDYP KIVIDRVAN RSQMENFPQ 
      SRLPQLTTA EKLKIRGTY DFLGINHYI TLYSKAANE 
      PPFSKPSFK ADAGGERFR DENWKGGAA NWIKLVPWG 
      LRNLLKWIK VNYRNPEII ITENGTADD GSDFLEDDE 
      RIEYYREYL NATLQAIIQ DGVNVKGYM AWSLMDNYE 
      WTNGYTLRF GLYHVNFTD SNRTRTPKK SVKFFKSLT 
      STKTIPFES DKMILKGFT ILLVSLLIS LTRTAQWKF 
      PEDFKFGVA TSAYQIEGG WNEDGKSIS VWDRITHQT 
      PAFIVDQTS GDVACDSYH QWERDVEML EELGVHYYR 
      FSLAWTRIL SGAYTSKVN PAGVKYYNN LINKLIENG 
      IEPVVTLYH WDMPQKIST LGGLTNDAW VKFYTHYAR 
      TAFELFGDR VKIWITFNE PNIICHYFN RVLGSVQPA 
      YPNGVIEYL CSYNLLKAH AEAYHIYDR EFRHKQGGK 
      VGITLNFEW AEPATDKPE DVEAAERRR QFDFGIFAN 
      PIINFDYPK VVIDRVAKR SELEEFPES RLQKFTFAE 
      KLNVKGTYD FLGLNHYTT WLVSNGEEA PLDQSGFYI 
      DTKVNRVQD DSWNTTGAD DNRVVPWGL RKALNWIKT 
      TYGNPDILI TECGYSDKV GNLDDDQRV NFYRDYLNA 
      TLQAILEDG VNVKAFMAW SLMDNFEWN QGYLVKFGL 
      YHVDFSDAK RPRTMKDSA KYYQKIIAT RRLDQVV
    CDS sequence
    • >TC033705 RA
      atgatcaaa gttaaagtt tccatcgcc atgcacccc 
      acacatgcg aaaaatctc cttttccga ctttgcgtt 
      ttcggcact ttaatcagt ctagctaaa actcaatgg 
      accttccct gataatttc aaatttggc gtcgccacg 
      tccgcgtac cagatcgaa ggtggttgg gatgccgat 
      ggcaaaggt gtgagcaca tgggacaga ctcacccat 
      aatacaccc ggaatgata caagatgga tccaacgga 
      gatatcgcg tgcgactct taccataaa tgggagcga 
      gatgtggaa atggtgaag gagacggga gtggactat 
      taccggttt tcgctatct tggacgaga attttccct 
      cagggatac atcaatctg gttaaccag cccggagtc 
      gattattac aataatctg attaataaa ttaatcgag 
      aatgggatc gagccggtc attactttg taccactgg 
      gacttgccg caaatgttt agccctttg ggatcatgg 
      gcgagtcct gtgatggtc gacctgttc gggaattat 
      gccagaaaa gccttccaa ctctttgga gaccgggtc 
      aagacttgg atcaccttc aacgaaccg aaaattgtc 
      tgccaggac tttcacgac tttttggga aatgtcaca 
      agcccatat cccaaaggg atcatcgag tacttgtgc 
      acccataac ttgctcaaa gcccatgcc gaagcgtac 
      catatttac gacaaagag tttcgacca acccagaag 
      ggaagaatc tccataact ctcaatttt gaatgggcc 
      gagcctgcc tctgacaat attgaagat atcgaagca 
      gcagagcag agacgacaa tttgaattt ggtctttat 
      gccaaccca attataaat tttgattat cccaaaatt 
      gttaaggat cgaattgct gaacgaagt aggaaggaa 
      ggttatccc aaatcgcga ctgcccgaa tttactttg 
      gccgaaaaa ctcaaactt agaggaact tatgattac 
      ttgggattg aatcattat agtacttgg tacgtcaaa 
      gcagtcgaa gatcaaccc ataggcact cccagttat 
      ccagctgat atgggtacg gagcggtac caagaccca 
      acttgggaa ggatctggc gctgactgg aataaagtt 
      gtcccatgg ggtttgcgg cacatttta cagtggatt 
      aaaaaaacc tacaggaac cctattgtt ttaattacg 
      gaatgcgga tatcctgac agaactggt accgtagag 
      gatgagcca agaatagac tttttcaga aaatatcta 
      aatgccact cttgaggca atttacgaa gacggtgct 
      aatgttcaa gcattcatg gcttggagt ttaatggat 
      aatttcgaa tggcaacaa ggctatcag ttatcaaca 
      gccaaatgg aaattcccc gacgatttt aaattcgga 
      gttgcgact tcggcctac cagacagaa ggtggctgg 
      aatgccgac ggcagaggt accagcata tgggacgtg 
      gccgcccat aatcgctcg tttctgatc cgcgaccgc 
      agtaacggc gacatagcc tgtgattcg tataataaa 
      tgggaggag gacgtcctc atggtcaaa aatctggga 
      gtgcactat taccgattc tccttgtcc tggacgaga 
      atcctgcct gatgggtac tcctccaaa gttaaccca 
      gagggagtg aaatattac aacaaaatc atcgataaa 
      ctaatcgaa aatcagata gagccggtc gtcactttg 
      taccactgg gacttgcca caagctttc agtgctttg 
      ggtggatgg actaatcct attgtgatc ccacacttt 
      gcaaacttt gcccgaaaa gccttccgt ttattcggg 
      gatagagtc aaaatctgg attacgata aacgaacca 
      cgactaatg tgcgatttt ttcaaagga ttagttggt 
      gacataacc cctgattat cctttagga atctcggaa 
      tatttgtgt gcccacaat gtcctaaag gcacatgcg 
      gccgcttat catatttac gataaagaa ttcagacct 
      atccagcac ggaaaaatc tcaatcact ttggacatg 
      gtgtggtca gaaccggca aatgccagt gaccctgaa 
      gacgtacga gctgcagaa caaaacgtg cagttcgat 
      tttggtctt tacgctaac ccgattttt aactacgat 
      tacccaaaa gttgttatt gaacgtgtg gcaaatcga 
      agtaaaata gaaaggatc cctaaatca agactaccg 
      aaattcaca atggcggaa aaattaaaa attagtggg 
      acttatgat tttttagga ataaatcat tatatcaca 
      atgtacact aaagcgacc aaagaacca ccggtgact 
      aaaccatcg ttcgatgta gattcagga ggaaaaagg 
      ttttttgat cccaagtgg gaaggttgt gctgctgat 
      tggattaaa gtagtaccg tggggctac agaaacagt 
      ttaaaatgg attaaaaag aattaccga aacccggaa 
      attattata acagaaaat ggttatgct gacggtggc 
      gacgtcatt aatgacgat gggagaatt aattactat 
      cgagaatac ttgaatgcc actctacaa gcaattata 
      gaagatggt gtcaatgtc aagagttac attgcttgg 
      agtttgatg gacaattat gagtggggt tttggttat 
      actttttcc accgctata gaatggaaa ttccctgct 
      gatttcaaa ttcggagtc gccacggcg gcctaccag 
      atcgaaggt ggctggaat gccgatggt aaaggtacc 
      agcatatgg gacactctg gggcataac cgctccatc 
      ctcgtaaaa gaccgcagc aacggtgac atagcttgc 
      gattcatac cacaaatgg gaggaggac gtccaaatg 
      gtcaaaaat ctgggcgtt gacacttat cggttttct 
      gtagcttgg actagaatt ctaccagaa ggttacgcc 
      acaagggtg aaccagcca ggagtcgac tattacaac 
      aaactgatt aataagttg atcgaaaat gggattgaa 
      ccggtcgtt actttgtac cattgggac ttgcctcaa 
      gttttcagc cctttgggg ggatggacc aatccagtg 
      gttgtccca cattttgcg aactatgcc agaaaggca 
      tttgaattg tttggggat agggtcaag acgtggatt 
      acgataaat gagccgaga ttaatttgc aacgaatac 
      aaagggcta gttggagac ataacagag gattttcct 
      ttaggggta tctgagtat ttgtgtgcc aagaacgta 
      ctaaaagca cacgctgaa gcttaccac atctacgat 
      aaagaattc agaaaaaca caaaaaggc aaaatctcg 
      ataactcta gacatgacg tggtcagaa cccgcaaca 
      aataatcct gaagatgtc aaagcagcc gaacaaaac 
      cgtcagttt gattttggt atttacact aacccgatt 
      tttaattat gattatcca aaaattgtc attgaccgt 
      gtggcaaac cgaagtcaa atggaaaat tttccacaa 
      tcgagactc ccgcaactt accaccgct gaaaaatta 
      aaaattcgt ggtacttac gacttcttg ggcataaac 
      cattacatt accttgtat tctaaagcc gctaacgaa 
      ccacccttt tccaaacca tcgttcaaa gctgacgct 
      ggaggtgaa aggtttcgg gacgaaaat tggaaaggt 
      ggcgcggca aattggatt aaacttgtc ccttggggt 
      ttacgaaac cttttaaaa tggattaaa gtaaactac 
      cgtaacccg gaaatcata ataacggaa aatggcacc 
      gctgacgat ggttcagat tttttagaa gatgatgaa 
      agaatcgaa tattatcga gaatatctc aatgcgact 
      ctacaagca attattcag gatggagtc aatgttaaa 
      ggctacatg gcgtggagt ttgatggac aactacgag 
      tggacaaat ggatacacg ctgagattt ggcctctac 
      catgtgaac ttcaccgat tccaaccgc acccgaaca 
      ccgaaaaaa tccgtcaag tttttcaaa agcctcaca 
      agtaccaaa accatacca tttgagtct gacaaaatg 
      atccttaaa ggtttcaca attttgctt gtatctctc 
      ctaatcagt ttgacccga actgcccaa tggaagttc 
      cctgaggac tttaagttc ggagttgca acttcggcc 
      tatcagatc gagggtgga tggaacgaa gatgggaaa 
      agtataagt gtctgggac cgaatcacc catcaaact 
      ccggctttt atcgtcgat cagacttcc ggggacgtt 
      gcctgcgat tcgtaccac caatgggaa cgcgatgtc 
      gaaatgttg gaggagttg ggggtccac tactaccgc 
      ttttccctt gcttggact cgcatcctg tccggggct 
      tacaccagc aaggtaaac ccagccgga gtcaaatat 
      tacaacaat ttgattaac aaactcatc gagaatggt 
      attgaaccc gtggtcact ctgtaccac tgggacatg 
      ccccagaaa attagcacc ttgggtggg ttaacaaac 
      gacgcgtgg gtcaagttc tacacacat tacgcacga 
      acggccttt gagttgttc ggagatcga gtcaaaatc 
      tggatcact ttcaacgaa ccgaacata atctgccat 
      tactttaat cgtgttctg ggcagtgtt cagccagct 
      tatcccaac ggagtcatc gaatatttg tgcagctac 
      aatctactt aaagcccac gctgaggct taccatatc 
      tacgaccgg gaatttcga cacaaacaa ggcggaaaa 
      gtcggtatt actcttaat tttgaatgg gcagaacct 
      gccactgac aaaccggaa gatgttgaa gcggctgaa 
      agaagacga cagtttgat tttgggatt tttgctaat 
      ccgataatt aacttcgat tatccaaaa gttgtgatt 
      gaccgagtt gccaaacga agcgagttg gaagagttt 
      ccggagtca agattacaa aaattcact tttgccgaa 
      aaattaaac gtgaaaggg acttatgac ttcctgggg 
      ttgaaccat tacaccact tggttggtt tcaaacgga 
      gaagaagcg cctttagac caatcaggt ttttatatc 
      gataccaaa gtaaacagg gtacaagat gatagttgg 
      aatacgaca ggagctgac gacaatcgg gtggtgcca 
      tggggtttg cgcaaagcc ttaaactgg ataaaaaca 
      acctatgga aaccctgac attcttatc acggagtgt 
      ggttattcc gataaagtc ggtaattta gatgacgac 
      caacgtgtt aatttttac agggattat ctaaatgca 
      actcttcaa gcaattttg gaagatgga gtgaatgtg 
      aaagccttc atggcttgg agtttgatg gataatttc 
      gaatggaat caaggatat ttagtgaaa ttcggattg 
      taccatgtt gacttcagc gatgccaaa aggccaagg 
      acaatgaag gattcagca aagtattat caaaaaatt 
      attgccacg agacgatta gaccaagtt gtgtaa
    mRNA sequence
    • >TC033705 RA
      ctatcctca tgatcaaag ttaaagttt ccatcgcca 
      tgcacccca cacatgcga aaaatctcc ttttccgac 
      tttgcgttt tcggcactt taatcagtc tagctaaaa 
      ctcaatgga ccttccctg ataatttca aatttggcg 
      tcgccacgt ccgcgtacc agatcgaag gtggttggg 
      atgccgatg gcaaaggtg tgagcacat gggacagac 
      tcacccata atacacccg gaatgatac aagatggat 
      ccaacggag atatcgcgt gcgactctt accataaat 
      gggagcgag atgtggaaa tggtgaagg agacgggag 
      tggactatt accggtttt cgctatctt ggacgagaa 
      ttttccctc agggataca tcaatctgg ttaaccagc 
      ccggagtcg attattaca ataatctga ttaataaat 
      taatcgaga atgggatcg agccggtca ttactttgt 
      accactggg acttgccgc aaatgttta gccctttgg 
      gatcatggg cgagtcctg tgatggtcg acctgttcg 
      ggaattatg ccagaaaag ccttccaac tctttggag 
      accgggtca agacttgga tcaccttca acgaaccga 
      aaattgtct gccaggact ttcacgact ttttgggaa 
      atgtcacaa gcccatatc ccaaaggga tcatcgagt 
      acttgtgca cccataact tgctcaaag cccatgccg 
      aagcgtacc atatttacg acaaagagt ttcgaccaa 
      cccagaagg gaagaatct ccataactc tcaattttg 
      aatgggccg agcctgcct ctgacaata ttgaagata 
      tcgaagcag cagagcaga gacgacaat ttgaatttg 
      gtctttatg ccaacccaa ttataaatt ttgattatc 
      ccaaaattg ttaaggatc gaattgctg aacgaagta 
      ggaaggaag gttatccca aatcgcgac tgcccgaat 
      ttactttgg ccgaaaaac tcaaactta gaggaactt 
      atgattact tgggattga atcattata gtacttggt 
      acgtcaaag cagtcgaag atcaaccca taggcactc 
      ccagttatc cagctgata tgggtacgg agcggtacc 
      aagacccaa cttgggaag gatctggcg ctgactgga 
      ataaagttg tcccatggg gtttgcggc acattttac 
      agtggatta aaaaaacct acaggaacc ctattgttt 
      taattacgg aatgcggat atcctgaca gaactggta 
      ccgtagagg atgagccaa gaatagact ttttcagaa 
      aatatctaa atgccactc ttgaggcaa tttacgaag 
      acggtgcta atgttcaag cattcatgg cttggagtt 
      taatggata atttcgaat ggcaacaag gctatcagt 
      tatcaacag ccaaatgga aattccccg acgatttta 
      aattcggag ttgcgactt cggcctacc agacagaag 
      gtggctgga atgccgacg gcagaggta ccagcatat 
      gggacgtgg ccgcccata atcgctcgt ttctgatcc 
      gcgaccgca gtaacggcg acatagcct gtgattcgt 
      ataataaat gggaggagg acgtcctca tggtcaaaa 
      atctgggag tgcactatt accgattct ccttgtcct 
      ggacgagaa tcctgcctg atgggtact cctccaaag 
      ttaacccag agggagtga aatattaca acaaaatca 
      tcgataaac taatcgaaa atcagatag agccggtcg 
      tcactttgt accactggg acttgccac aagctttca 
      gtgctttgg gtggatgga ctaatccta ttgtgatcc 
      cacactttg caaactttg cccgaaaag ccttccgtt 
      tattcgggg atagagtca aaatctgga ttacgataa 
      acgaaccac gactaatgt gcgattttt tcaaaggat 
      tagttggtg acataaccc ctgattatc ctttaggaa 
      tctcggaat atttgtgtg cccacaatg tcctaaagg 
      cacatgcgg ccgcttatc atatttacg ataaagaat 
      tcagaccta tccagcacg gaaaaatct caatcactt 
      tggacatgg tgtggtcag aaccggcaa atgccagtg 
      accctgaag acgtacgag ctgcagaac aaaacgtgc 
      agttcgatt ttggtcttt acgctaacc cgattttta 
      actacgatt acccaaaag ttgttattg aacgtgtgg 
      caaatcgaa gtaaaatag aaaggatcc ctaaatcaa 
      gactaccga aattcacaa tggcggaaa aattaaaaa 
      ttagtggga cttatgatt ttttaggaa taaatcatt 
      atatcacaa tgtacacta aagcgacca aagaaccac 
      cggtgacta aaccatcgt tcgatgtag attcaggag 
      gaaaaaggt tttttgatc ccaagtggg aaggttgtg 
      ctgctgatt ggattaaag tagtaccgt ggggctaca 
      gaaacagtt taaaatgga ttaaaaaga attaccgaa 
      acccggaaa ttattataa cagaaaatg gttatgctg 
      acggtggcg acgtcatta atgacgatg ggagaatta 
      attactatc gagaatact tgaatgcca ctctacaag 
      caattatag aagatggtg tcaatgtca agagttaca 
      ttgcttgga gtttgatgg acaattatg agtggggtt 
      ttggttata ctttttcca ccgctatag aatggaaat 
      tccctgctg atttcaaat tcggagtcg ccacggcgg 
      cctaccaga tcgaaggtg gctggaatg ccgatggta 
      aaggtacca gcatatggg acactctgg ggcataacc 
      gctccatcc tcgtaaaag accgcagca acggtgaca 
      tagcttgcg attcatacc acaaatggg aggaggacg 
      tccaaatgg tcaaaaatc tgggcgttg acacttatc 
      ggttttctg tagcttgga ctagaattc taccagaag 
      gttacgcca caagggtga accagccag gagtcgact 
      attacaaca aactgatta ataagttga tcgaaaatg 
      ggattgaac cggtcgtta ctttgtacc attgggact 
      tgcctcaag ttttcagcc ctttggggg gatggacca 
      atccagtgg ttgtcccac attttgcga actatgcca 
      gaaaggcat ttgaattgt ttggggata gggtcaaga 
      cgtggatta cgataaatg agccgagat taatttgca 
      acgaataca aagggctag ttggagaca taacagagg 
      attttcctt taggggtat ctgagtatt tgtgtgcca 
      agaacgtac taaaagcac acgctgaag cttaccaca 
      tctacgata aagaattca gaaaaacac aaaaaggca 
      aaatctcga taactctag acatgacgt ggtcagaac 
      ccgcaacaa ataatcctg aagatgtca aagcagccg 
      aacaaaacc gtcagtttg attttggta tttacacta 
      acccgattt ttaattatg attatccaa aaattgtca 
      ttgaccgtg tggcaaacc gaagtcaaa tggaaaatt 
      ttccacaat cgagactcc cgcaactta ccaccgctg 
      aaaaattaa aaattcgtg gtacttacg acttcttgg 
      gcataaacc attacatta ccttgtatt ctaaagccg 
      ctaacgaac caccctttt ccaaaccat cgttcaaag 
      ctgacgctg gaggtgaaa ggtttcggg acgaaaatt 
      ggaaaggtg gcgcggcaa attggatta aacttgtcc 
      cttggggtt tacgaaacc ttttaaaat ggattaaag 
      taaactacc gtaacccgg aaatcataa taacggaaa 
      atggcaccg ctgacgatg gttcagatt ttttagaag 
      atgatgaaa gaatcgaat attatcgag aatatctca 
      atgcgactc tacaagcaa ttattcagg atggagtca 
      atgttaaag gctacatgg cgtggagtt tgatggaca 
      actacgagt ggacaaatg gatacacgc tgagatttg 
      gcctctacc atgtgaact tcaccgatt ccaaccgca 
      cccgaacac cgaaaaaat ccgtcaagt ttttcaaaa 
      gcctcacaa gtaccaaaa ccataccat ttgagtctg 
      acaaaatga tccttaaag gtttcacaa ttttgcttg 
      tatctctcc taatcagtt tgacccgaa ctgcccaat 
      ggaagttcc ctgaggact ttaagttcg gagttgcaa 
      cttcggcct atcagatcg agggtggat ggaacgaag 
      atgggaaaa gtataagtg tctgggacc gaatcaccc 
      atcaaactc cggctttta tcgtcgatc agacttccg 
      gggacgttg cctgcgatt cgtaccacc aatgggaac 
      gcgatgtcg aaatgttgg aggagttgg gggtccact 
      actaccgct tttcccttg cttggactc gcatcctgt 
      ccggggctt acaccagca aggtaaacc cagccggag 
      tcaaatatt acaacaatt tgattaaca aactcatcg 
      agaatggta ttgaacccg tggtcactc tgtaccact 
      gggacatgc cccagaaaa ttagcacct tgggtgggt 
      taacaaacg acgcgtggg tcaagttct acacacatt 
      acgcacgaa cggcctttg agttgttcg gagatcgag 
      tcaaaatct ggatcactt tcaacgaac cgaacataa 
      tctgccatt actttaatc gtgttctgg gcagtgttc 
      agccagctt atcccaacg gagtcatcg aatatttgt 
      gcagctaca atctactta aagcccacg ctgaggctt 
      accatatct acgaccggg aatttcgac acaaacaag 
      gcggaaaag tcggtatta ctcttaatt ttgaatggg 
      cagaacctg ccactgaca aaccggaag atgttgaag 
      cggctgaaa gaagacgac agtttgatt ttgggattt 
      ttgctaatc cgataatta acttcgatt atccaaaag 
      ttgtgattg accgagttg ccaaacgaa gcgagttgg 
      aagagtttc cggagtcaa gattacaaa aattcactt 
      ttgccgaaa aattaaacg tgaaaggga cttatgact 
      tcctggggt tgaaccatt acaccactt ggttggttt 
      caaacggag aagaagcgc ctttagacc aatcaggtt 
      tttatatcg ataccaaag taaacaggg tacaagatg 
      atagttgga atacgacag gagctgacg acaatcggg 
      tggtgccat ggggtttgc gcaaagcct taaactgga 
      taaaaacaa cctatggaa accctgaca ttcttatca 
      cggagtgtg gttattccg ataaagtcg gtaatttag 
      atgacgacc aacgtgtta atttttaca gggattatc 
      taaatgcaa ctcttcaag caattttgg aagatggag 
      tgaatgtga aagccttca tggcttgga gtttgatgg 
      ataatttcg aatggaatc aaggatatt tagtgaaat 
      tcggattgt accatgttg acttcagcg atgccaaaa 
      ggccaagga caatgaagg attcagcaa agtattatc 
      aaaaaatta ttgccacga gacgattag accaagttg 
      tgtaaactg tagctctct ttctgacaa taaaattta 
      aacacagca
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