Status
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Gene information
General information
TC008998 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8949068..8973155
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008998
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04573

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008998 RA
      MAADSGMDT CPSPEIADS RKRPLDGDT DNGDTKRSH 
      FSPGGDGTY HFKVLVPSV AAGAIIGKG GETIAQLQK 
      ETGARVKMS KSHDFYPGT TERVCLITG SVDAIMVVA 
      DFIADKIRE KPDLTKPLV ETESKLMQD RDKQVKILV 
      PNSTAGMII GKAGNYIKQ IKETSGSYV QISQKAKDV 
      SLQERCITV IGEKEQNRQ ACQMILAKI VEDPQSGTC 
      LNVSYADVN GPVANYNPT GSPFAQQNA GSAFNASTA 
      SLNSGLSSV SQSIMLNGG LNLSLNLNA PGQNVGTVT 
      AQLLDHIKL QLRNTGFSD SHIVEICAA LSVLAKYGI 
      LAMGLGLGS AHTGALPVS SYLGVMDQA SAATANTGA 
      VFGPIGGQV SLAECHLGT TSPTPRSNI ERYDSTFDP 
      FRHQSSPAT APISINNNS FGLGTGQQM AVLSKSPTP 
      AELNGAKDS KNVEIAEVI VGAILGPGG RSLVEIQQL 
      SGANIQISK KGIFAPGTR NRIVTITGS PQSINTAQY 
      LIEQRISEE ELKRARNNG LGTLTMQ
    CDS sequence
    • >TC008998 RA
      atggccgcc gattcggga atggatacg tgcccgtcg 
      ccagagata gccgactcc aggaagagg ccgctggac 
      ggtgacacc gacaacggc gacaccaag cgctcccac 
      ttcagtcca ggcggagat ggaacgtat cacttcaaa 
      gtcctggtg ccgagtgtg gcggctggc gccatcatc 
      ggcaagggc ggcgagacc atcgcccaa ctccagaag 
      gaaacgggg gctcgcgtc aagatgtcc aaatcgcac 
      gacttctac cccggaacg acggaacga gtgtgcctc 
      ataaccggt agcgtcgac gctataatg gtagttgca 
      gactttata gcagataag attcgcgaa aaacccgac 
      ttgaccaaa cctttagta gaaaccgaa agtaaatta 
      atgcaagac agagacaaa caagtgaaa atattagtg 
      ccgaattcg acagcagga atgatcatc ggcaaggcg 
      ggtaactac attaagcaa attaaagag accagcggt 
      tcttacgtg cagatttct cagaaggcc aaggatgtt 
      agtttgcaa gaaagatgt ataactgtt ataggtgaa 
      aaagaacaa aacagacaa gcgtgtcag atgatatta 
      gcaaaaatc gtcgaggac ccccagagc ggaacgtgt 
      ctgaatgta tcgtacgcg gacgtgaac ggtccagtg 
      gccaattat aaccccacg ggctcgccc ttcgcccaa 
      cagaacgct ggaagcgcg ttcaacgcg tccacggcg 
      agtctgaac agcggactc agttcagtg tcccagagt 
      atcatgctc aacggagga ctcaatctc agcctaaac 
      ctcaacgct ccgggacag aacgtcggc accgtcacg 
      gctcagctg ctggaccac atcaaactg caactgaga 
      aacaccggt ttttccgac tcccatatc gtcgagatt 
      tgcgccgct ctgtccgtg ttggcaaag tacggcatc 
      ctggccatg ggtctgggg ctggggagc gcccacacg 
      ggggcgttg cccgtttcg agttaccta ggtgtgatg 
      gaccaagcc agcgctgcc acagccaac accggagcc 
      gtcttcggg cctattggg ggccaggtc agtttagca 
      gaatgccat ttgggtacc acgtcccca actccacga 
      tccaatata gaaaggtat gattcaaca ttcgatcca 
      ttcagacat caaagttca cccgcaaca gcacccatc 
      tcaatcaac aataacagc tttggtcta ggtacaggt 
      caacaaatg gctgtgcta agtaaaagt cccactcct 
      gcggaatta aatggtgct aaagactct aaaaatgtc 
      gaaattgca gaagtaatt gttggtgct attttaggt 
      cctgggggc cgttcgtta gttgaaatc cagcagctg 
      agcggggcc aacattcag atatcgaag aagggtata 
      ttcgcaccg ggaactcgc aatagaata gtgactata 
      acgggtagt ccgcaatcg ataaacacg gcccaatat 
      ctgatcgaa caacgcatc tccgaagag gaactgaaa 
      agggcacgg aacaacggc ctcggtact ctaaccatg 
      cagtga
    mRNA sequence
    • >TC008998 RA
      tgctgttat gataaggtt tttctccca ttacgtaac 
      tgccattat cgcgttgta aaatcggaa taaatcaca 
      gctggccgt aattcgtca aaagtgtca tttagtcaa 
      cgtaaaatt tttgtttgt gtcatttgg tgcttccca 
      ttaaatcca tttcgacat tttttcggt aaattcagg 
      gacacatct tcgcttaac ggaaaattc accagtgtc 
      ggcggagtc tgcgaaatt agtcgtgtt gtatgtgtt 
      ctgttggtt cagtcagtt gcagtccgg tgtcctgtc 
      gtaatcgtc gtatcctgt ccgccgtct ccgaatccg 
      aaaataggc cgacctggt gcgcgcatc ttcaccata 
      gctccttcg ttcccaagg ccaagtggg gggacgcta 
      tctaatcac cgccccgtg acatgtcgc tgattcagt 
      ctgcgacga gcccgtgag cagtgtgga ctgtcgttc 
      ctctccgag agctctccc tgcagctgg tgcgcgagt 
      gttgtcagt gcgcagtgt cgtaacata gaaatcaca 
      cgtaaagcg gtccgctct gcgctgatg gacgcctga 
      acgttggca aaatggccg ccgattcgg gaatggata 
      cgtgcccgt cgccagaga tagccgact ccaggaaga 
      ggccgctgg acggtgaca ccgacaacg gcgacacca 
      agcgctccc acttcagtc caggcggag atggaacgt 
      atcacttca aagtcctgg tgccgagtg tggcggctg 
      gcgccatca tcggcaagg gcggcgaga ccatcgccc 
      aactccaga aggaaacgg gggctcgcg tcaagatgt 
      ccaaatcgc acgacttct accccggaa cgacggaac 
      gagtgtgcc tcataaccg gtagcgtcg acgctataa 
      tggtagttg cagacttta tagcagata agattcgcg 
      aaaaacccg acttgacca aacctttag tagaaaccg 
      aaagtaaat taatgcaag acagagaca aacaagtga 
      aaatattag tgccgaatt cgacagcag gaatgatca 
      tcggcaagg cgggtaact acattaagc aaattaaag 
      agaccagcg gttcttacg tgcagattt ctcagaagg 
      ccaaggatg ttagtttgc aagaaagat gtataactg 
      ttataggtg aaaaagaac aaaacagac aagcgtgtc 
      agatgatat tagcaaaaa tcgtcgagg acccccaga 
      gcggaacgt gtctgaatg tatcgtacg cggacgtga 
      acggtccag tggccaatt ataacccca cgggctcgc 
      ccttcgccc aacagaacg ctggaagcg cgttcaacg 
      cgtccacgg cgagtctga acagcggac tcagttcag 
      tgtcccaga gtatcatgc tcaacggag gactcaatc 
      tcagcctaa acctcaacg ctccgggac agaacgtcg 
      gcaccgtca cggctcagc tgctggacc acatcaaac 
      tgcaactga gaaacaccg gtttttccg actcccata 
      tcgtcgaga tttgcgccg ctctgtccg tgttggcaa 
      agtacggca tcctggcca tgggtctgg ggctgggga 
      gcgcccaca cgggggcgt tgcccgttt cgagttacc 
      taggtgtga tggaccaag ccagcgctg ccacagcca 
      acaccggag ccgtcttcg ggcctattg ggggccagg 
      tcagtttag cagaatgcc atttgggta ccacgtccc 
      caactccac gatccaata tagaaaggt atgattcaa 
      cattcgatc cattcagac atcaaagtt cacccgcaa 
      cagcaccca tctcaatca acaataaca gctttggtc 
      taggtacag gtcaacaaa tggctgtgc taagtaaaa 
      gtcccactc ctgcggaat taaatggtg ctaaagact 
      ctaaaaatg tcgaaattg cagaagtaa ttgttggtg 
      ctattttag gtcctgggg gccgttcgt tagttgaaa 
      tccagcagc tgagcgggg ccaacattc agatatcga 
      agaagggta tattcgcac cgggaactc gcaatagaa 
      tagtgacta taacgggta gtccgcaat cgataaaca 
      cggcccaat atctgatcg aacaacgca tctccgaag 
      aggaactga aaagggcac ggaacaacg gcctcggta 
      ctctaacca tgcagtgat ggcgtcaaa ccgttttaa 
      attcaccca gattgttca ggtacacgc aacagagat 
      tgttaatgt tttagaaga gaaaactaa aaatagtaa 
      aaatggtaa caaattttt atttacgat ttaaatgtg 
      taataggga gacatttta aatagtttg ttgttgaat 
      atttttaac gaattgaaa gttatttat gatgatgac 
      tcaaggtaa attgcgcaa ggctgttta tatacaatg 
      tgaactttt taaaagatg tatacctat ttacataat 
      tgtttgtac tgtaccgcg gggtagtaa tggaaattt 
      tttatagtc aattccttg aaaatgtga tgtgatttg 
      ttattattt atttttatt tgttttgtt tttattatc 
      taaatatta gacattgta agggcgagt cttgcctgg 
      ccatgtcgt ttcttcccc agcaaggct cgcctcgac 
      cgccagtaa attttcgtt atgttctgt ttgatgtat 
      tcgagagtt taatgatta cacgaaaca aatttgagg 
      taaatgatc tgatttacg agatttaga atagtgcta 
      cattctaat tgagaaaaa attgtgatt cttcattca 
      ttcacatca ttatcaaca ctctgatgt ttgtaccca 
      tatgctttg tttttgttt tattttctt tttcttttt 
      gtacatata tacataatt ttccttttg atagttgtt 
      gcggagcgg tgcgcgtcg aggcgtgcg aggtagttt 
      aattagaag agatgtgtg catattatg tgattggta 
      atagcagtc attcgagca attcctaaa tttcgtacc 
      tgctgaaga acctcatcc atcaatatg acaagcgac 
      atttggctg gccaaagag catattgaa tttatttag 
      atgaaaata tttaaaatc cgttaccaa tcatgagat 
      tttattttt ggggtttcg gccgaagag gcatgacat 
      tgtaaatag tcatagcgt tagtcatct atactcacc 
      caactctgc tgttattcc ttctataga tctatatat 
      tattattaa aaagaatat atatgaaga taaatgtaa 
      ctaaaaatt atatattgt tgttatcgc atgttgtta 
      atattgtta atgtttgga cactcgctg ctgaatatt 
      taacaaagc cgttgacta atcgactta catgcgtta 
      acttggaaa tattttatt tttaagttt aatttagcg 
      gagatctat gcaggtcat agacgtgtt tcaccgaat 
      gacgtggta agctgtggc attccaatt tagcgaaag 
      aaaacgtac catacagag tttatatag ttttgttac 
      gaatgtttt attttttta tttttcgat tgttgacgg 
      aatagttta aatattaag atgccgtta acacagagc 
      atagtttca ttgtctaaa tgtatgtga tcgccgtag 
      tttcataat gttgagtca aagatatta atattctat 
      aaacgattg ttcaagatt tgtgcgctt gtaaatcaa 
      agatttaaa aataatatt tggtctata tttcatgtt 
      aaaatcttt agatattta agaattgtt tcgttttta 
      gttgttgct tctccacgt tttaacata tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY