Status
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Gene information
General information
TC008984 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9279438..9281022
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008984
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04571

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008984 RA
      MDQESDKIL KHSTDVMQL FADKKIVKI CAPMVRYSK 
      LQFRNLVKS YNCDLCYTP MILADSFCK SGKARANEF 
      TTNMNDTPL ITQFAANKV YDFVGAAYM VSPFCDGVD 
      LNCGCPQRW AKLQGLGCI MLEKPEVIY DLVKQCRNV 
      IPKPFTVSV KLRLQKDLR RSVEICRQL EHCGVSFLT 
      VHGRTPDQL TGEVNKEAL NLIVGSVNV PVVANGGVK 
      SLEDCLELQ EKTNCKGVM VANGLLTNP TLFTESSVT 
      TTDCIQRWL NICYNTTLD YRDINRLHT IPEKPKNLT 
      FQCFHHHLV FMLEKILTR SEKRIFNNL QTFEDVLSY 
      IKNKFNLAP QLYDTDDFN NVKLLEVDY GKRGEYYFE 
      HKPKDENVE YDLFYDHES HGKYFKSKV EDDCNWSDI 
      FLENG
    CDS sequence
    • >TC008984 RA
      atggatcaa gaatcggat aaaatactg aaacattca 
      acagacgtg atgcagctt tttgcagac aagaaaata 
      gtaaaaatt tgcgccccc atggtgcgc tacagcaaa 
      ttacagttc cgaaatttg gtcaaatcc tacaattgc 
      gacctttgt tacacccct atgatcctt gcagattcg 
      ttctgcaag agtggcaaa gctagagcc aatgaattt 
      acaactaac atgaatgat acaccttta attacccaa 
      tttgcggcc aataaagtt tatgatttt gtgggagca 
      gcttacatg gtgtcccct ttttgtgac ggagttgac 
      ttaaactgt gggtgcccc caaagatgg gccaagctg 
      caaggttta ggttgtata atgttagaa aagcctgaa 
      gtaatttac gatttggtc aaacagtgt aggaatgtc 
      ataccaaag ccctttact gttagtgtt aaattgagg 
      ttgcaaaag gatttaaga agaagtgta gaaatttgt 
      agacaattg gaacattgt ggggttagt tttttaaca 
      gttcatgga cgtacgcct gaccaatta acaggggaa 
      gtgaataaa gaagctttg aatttaatt gtggggagt 
      gtaaatgtt ccagttgtt gctaacgga ggcgttaaa 
      tctttggag gactgtctg gagttgcaa gagaaaaca 
      aactgtaaa ggagttatg gtagccaat gggttactt 
      acaaacccc acattattc actgaatct tccgtcacc 
      actaccgat tgtattcaa aggtggctc aatatttgc 
      tacaacact acacttgat tacagagac attaacaga 
      cttcacaca attcccgaa aaacccaaa aatttaaca 
      ttccaatgt tttcatcac catttggtt tttatgcta 
      gaaaaaatt ctaacaaga tcagaaaag cgtattttt 
      aataatctt caaacattt gaagacgtt ttaagttat 
      attaaaaat aagtttaat ttggcgcca caattgtat 
      gatactgat gattttaat aacgtaaag ttacttgag 
      gtagactat ggtaaacga ggcgagtat tattttgaa 
      cacaaaccc aaggatgaa aatgtagaa tatgattta 
      ttttatgat catgaaagt cacggaaag tattttaaa 
      agtaaagtg gaagatgac tgtaattgg tctgatata 
      tttcttgaa aatggatga
    mRNA sequence
    • >TC008984 RA
      acctcaaaa aataaacca agtgattaa ttattatat 
      cgtaaaata ttaacgtta attgatttt tttaattct 
      caaaaattc atcgtttgt gctaaaaat atgcccctt 
      tgttaaact ggtctcact ttggtagtc accagtata 
      acctcactt caagaaatg gatcaagaa tcggataaa 
      atactgaaa cattcaaca gacgtgatg cagcttttt 
      gcagacaag aaaatagta aaaatttgc gcccccatg 
      gtgcgctac agcaaatta cagttccga aatttggtc 
      aaatcctac aattgcgac ctttgttac acccctatg 
      atccttgca gattcgttc tgcaagagt ggcaaagct 
      agagccaat gaatttaca actaacatg aatgataca 
      cctttaatt acccaattt gcggccaat aaagtttat 
      gattttgtg ggagcagct tacatggtg tcccctttt 
      tgtgacgga gttgactta aactgtggg tgcccccaa 
      agatgggcc aagctgcaa ggtttaggt tgtataatg 
      ttagaaaag cctgaagta atttacgat ttggtcaaa 
      cagtgtagg aatgtcata ccaaagccc tttactgtt 
      agtgttaaa ttgaggttg caaaaggat ttaagaaga 
      agtgtagaa atttgtaga caattggaa cattgtggg 
      gttagtttt ttaacagtt catggacgt acgcctgac 
      caattaaca ggggaagtg aataaagaa gctttgaat 
      ttaattgtg gggagtgta aatgttcca gttgttgct 
      aacggaggc gttaaatct ttggaggac tgtctggag 
      ttgcaagag aaaacaaac tgtaaagga gttatggta 
      gccaatggg ttacttaca aaccccaca ttattcact 
      gaatcttcc gtcaccact accgattgt attcaaagg 
      tggctcaat atttgctac aacactaca cttgattac 
      agagacatt aacagactt cacacaatt cccgaaaaa 
      cccaaaaat ttaacattc caatgtttt catcaccat 
      ttggttttt atgctagaa aaaattcta acaagatca 
      gaaaagcgt atttttaat aatcttcaa acatttgaa 
      gacgtttta agttatatt aaaaataag tttaatttg 
      gcgccacaa ttgtatgat actgatgat tttaataac 
      gtaaagtta cttgaggta gactatggt aaacgaggc 
      gagtattat tttgaacac aaacccaag gatgaaaat 
      gtagaatat gatttattt tatgatcat gaaagtcac 
      ggaaagtat tttaaaagt aaagtggaa gatgactgt 
      aattggtct gatatattt cttgaaaat ggatgagga 
      aataaatga tctaatcta ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY