Status
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Gene information
General information
TC008959 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12709493..12716100
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008959
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04568

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008959 RA
      MMETQQWPA KFQQSGAGT DTTQDSLNN GEDGNVAKE 
      ENGDGVHES DKRAENDSP TISEKARQL ISLILEQMK 
      ELSNAEKYL LYLKLFQIS NVVDPLRQP LNPLGSRSE 
      INRTISWIK THLEEDAAL SLPKQEVYE EYTVYCTQN 
      QIKSLSQAD FGKVMKQVY PKVRARRLG TRGNSRYCY 
      SGLRRCIKL KPPKLPDLG DKPLSTEAP CTQSTLTAA 
      AWLIVKEWS EFHFGVQFP SLQALARFL VVSHSVGAG 
      TDAANQLTS ATESQLKGE VCGGKTGTK HREMQLQLQ 
      KKIQQKNEV KERKRKIQS PKSDPKPTV KKSKGSVSS 
      IRLEGSPTL TGAGESSTS TSTGSTSTS PTQGKSICD 
      KSLDFTQLP ALPDFKSFQ KPVCEQQQP QQQQQNGVG 
      TSTKVAIPR LASGKAQQQ QRSPQSSPK KQVKNAKYK 
      AIQPKPQPC DNASYNPQT TADIRSQGV LHNVDRCKE 
      RKSSDDDCD APVFPLPRE RLESISNVD KDAMDEYLG 
      TNNSQHEEE LSKYFSNNI ETTDQDNST KLSQLRQLL 
      EQNGISDNK NFLIDSGPT VALPHPMLD PLAVPNVNI 
      YPIPAQLST LPTNQSSAR RRVSFETPL HEDTVPPSP 
      NTRRKNFSF TPISPGPQS PTGRQSKCS STNVSPFVS 
      PRNTPIPRT KNTTHQNAG IGVRKKIKR ESDLSVEIP 
      DAKNYMAMS APVSPMLYN NKKSVLEKL LHSNSKVAY 
      TPDYVNPSV KHDVSEVDL LESENVETF PDFTAYRSQ 
      SVPLNQMIK SNFTEDAHF LPTLPNQGA DFNDFDPIS 
      ESVTVNRII DALDEHPCE NPGTMEMYN VEMPQNSQC 
      LPSFNLIVD NNIDNFGEN MAFAPGSRH LARSQSIDV 
      NCFDMKSAN LNPSRSVPS TPLPFNSSK PAPVKNFAN 
      QSSRSYPST PSLVAESAF NYAVNGDCL LNGQPIRNG 
      TARNQADEL TYFMNLNET NEDNNIDRH LTAEEEFSI 
      GRSLPGNEF GIAESENAL MDEDGVLIG EQTFNGNIN 
      N
    CDS sequence
    • >TC008959 RA
      atgatggaa actcaacaa tggcccgca aaattccaa 
      caatctggg gccgggacc gacaccaca caagatagt 
      ttaaacaat ggagaggac ggcaacgtc gcgaaagaa 
      gaaaacggc gatggggtg cacgagagc gacaagcgg 
      gccgaaaac gacagcccc acaatcagt gagaaagcc 
      agacagtta atatcgttg atcttagag cagatgaaa 
      gaattgtca aacgccgaa aaatatctc ttgtatttg 
      aaactgttt caaatcagc aatgtagtg gaccctttg 
      cggcaaccc ttgaaccca ctgggcagt cgttccgaa 
      atcaacagg accatatca tggattaag actcactta 
      gaagaagac gcagcgctt tctctcccc aagcaggag 
      gtctacgag gaatataca gtatattgc acacaaaat 
      caaatcaag tctctttcc caagctgat tttggcaag 
      gtcatgaag caagtgtat ccaaaagtg agggctagg 
      cgcttgggt acccgcgga aattccaga tattgctat 
      tcaggcttg agaagatgt atcaagtta aagcctcca 
      aaattgcca gatcttggc gataagcct ttaagcacg 
      gaagcccct tgtactcag tcgacttta acagcggcg 
      gcttggtta attgtgaaa gaatggtct gaatttcat 
      tttggagtt cagtttcca tcattgcaa gccttggcc 
      cggtttctt gttgtttct cattcagtt ggtgcagga 
      actgacgct gccaaccag ttgacttca gctacagaa 
      tcacagttg aaaggggag gtgtgtggt gggaaaact 
      ggaaccaaa cacagagaa atgcagctg cagttgcag 
      aagaagatc caacagaag aacgaagtc aaagaaaga 
      aaaagaaaa atacagtct cccaaatcc gatccgaag 
      cccactgtg aagaaatca aagggatca gtgtcgtcg 
      atccgactc gaaggttcc cccacattg acgggtgcg 
      ggtgaaagc agcaccagt acaagcacc ggcagcacc 
      agcacgagt cccactcag ggcaaatct atctgtgat 
      aaatcgtta gatttcact caactacca gcgttacct 
      gactttaaa agtttccaa aagcccgtc tgtgaacaa 
      caacaacca caacagcaa caacaaaac ggtgtcggg 
      acctcgacg aaagtagcg ataccgcgt ctagcctcc 
      ggtaaagct caacaacag caacgatcg ccccaatcg 
      tcccccaag aagcaagtc aagaatgca aagtacaaa 
      gccatccaa cccaaaccg caaccatgt gataacgca 
      tcgtacaac ccgcagacc actgcggat atcagatct 
      caaggtgta ctacacaac gtagatagg tgtaaggaa 
      agaaaatca agtgatgat gattgtgat gctcccgtt 
      tttccgctg ccgagggag aggctcgaa agcatcagc 
      aatgtcgac aaggacgcc atggacgaa tatctaggt 
      acaaacaac agccagcac gaagaagag ctgtcgaaa 
      tacttcagc aacaacatc gagacaacg gaccaagac 
      aactccacc aaactctcc caactccgt caactcctc 
      gaacagaac ggcatatcc gacaacaaa aatttcctc 
      atcgatagc ggccccacc gtcgccttg ccccacccc 
      atgctcgac cctctggcc gtccccaac gtcaacatc 
      tatccaatc ccggcccag ctctccaca ttaccgacc 
      aaccagagc agcgctcgc cgtcgcgtc agcttcgaa 
      acgcccctc cacgaggac accgtccct cccagccca 
      aatactcgc cgcaaaaac ttcagcttc acccccata 
      tcaccgggc ccccaatcg cccaccggg cgccaatcc 
      aagtgttcc agcacgaac gtaagtcct tttgtgagt 
      ccccggaac acaccaata ccccgtact aaaaacacg 
      acccaccaa aacgccgga atcggcgtc cggaaaaaa 
      atcaaacgc gaatccgat ctgagcgtc gaaatcccg 
      gacgcgaaa aactacatg gccatgtcg gcccccgtc 
      agccccatg ctatacaac aacaaaaaa tcggtcctc 
      gaaaaactg ctacattcg aacagcaaa gtcgcctac 
      accccggat tatgtcaat ccttccgta aaacacgac 
      gtgagcgaa gtcgattta ctcgaaagc gaaaacgtg 
      gagactttc ccggacttc acagcctat aggtcgcag 
      tcggtccct ttgaaccag atgatcaag tcgaatttc 
      accgaagac gcccacttc ctgccgact ctgcccaat 
      cagggcgcc gatttcaac gacttcgac ccgatttcc 
      gaatcggtg accgttaac cggatcatt gacgccctc 
      gacgagcat ccgtgcgag aatccgggc actatggag 
      atgtacaac gtggaaatg cctcaaaac agccaatgc 
      ctgcccagc tttaacctc attgtcgac aacaacatc 
      gacaacttc ggcgagaat atggccttt gcgcccgga 
      tcgcgtcac ctggccagg tcgcaaagc atcgatgtc 
      aattgtttc gatatgaaa tcggccaat ctcaaccct 
      tccagatct gtcccaagc acgcccctc ccatttaac 
      agtagcaag cccgccccc gtgaaaaac ttcgccaat 
      cagagctcg cgctcgtac ccgtcgacg ccgtcgttg 
      gtggccgag tcggcgttt aactacgcc gttaacgga 
      gactgtctg ttgaacgga cagccgata cggaacgga 
      acggcgcgg aatcaggcg gatgagttg acgtatttt 
      atgaatttg aacgaaacg aacgaggac aataatatc 
      gataggcat ttaacggca gaggaggag tttagtata 
      gggaggagt ttgccaggt aatgagttc ggtattgcc 
      gaaagtgag aatgctttg atggacgag gacggtgtc 
      ttgattggt gagcagact tttaacggg aatattaat 
      aattga
    mRNA sequence
    • >TC008959 RA
      atggataaa aaacgttta gaagaacga aaggattgt 
      cgtccattg gagaccagc tgatttgag ttgacaata 
      aaatgttcc ggtcttgtc agcccgtat tggctggta 
      aagtgtcga aaaattgtg cggttcgtg ctgaaacca 
      actgaaata ggccaaaaa attgctggc gatgatgga 
      aactcaaca atggcccgc aaaattcca acaatctgg 
      ggccgggac cgacaccac acaagatag tttaaacaa 
      tggagagga cggcaacgt cgcgaaaga agaaaacgg 
      cgatggggt gcacgagag cgacaagcg ggccgaaaa 
      cgacagccc cacaatcag tgagaaagc cagacagtt 
      aatatcgtt gatcttaga gcagatgaa agaattgtc 
      aaacgccga aaaatatct cttgtattt gaaactgtt 
      tcaaatcag caatgtagt ggacccttt gcggcaacc 
      cttgaaccc actgggcag tcgttccga aatcaacag 
      gaccatatc atggattaa gactcactt agaagaaga 
      cgcagcgct ttctctccc caagcagga ggtctacga 
      ggaatatac agtatattg cacacaaaa tcaaatcaa 
      gtctctttc ccaagctga ttttggcaa ggtcatgaa 
      gcaagtgta tccaaaagt gagggctag gcgcttggg 
      tacccgcgg aaattccag atattgcta ttcaggctt 
      gagaagatg tatcaagtt aaagcctcc aaaattgcc 
      agatcttgg cgataagcc tttaagcac ggaagcccc 
      ttgtactca gtcgacttt aacagcggc ggcttggtt 
      aattgtgaa agaatggtc tgaatttca ttttggagt 
      tcagtttcc atcattgca agccttggc ccggtttct 
      tgttgtttc tcattcagt tggtgcagg aactgacgc 
      tgccaacca gttgacttc agctacaga atcacagtt 
      gaaagggga ggtgtgtgg tgggaaaac tggaaccaa 
      acacagaga aatgcagct gcagttgca gaagaagat 
      ccaacagaa gaacgaagt caaagaaag aaaaagaaa 
      aatacagtc tcccaaatc cgatccgaa gcccactgt 
      gaagaaatc aaagggatc agtgtcgtc gatccgact 
      cgaaggttc ccccacatt gacgggtgc gggtgaaag 
      cagcaccag tacaagcac cggcagcac cagcacgag 
      tcccactca gggcaaatc tatctgtga taaatcgtt 
      agatttcac tcaactacc agcgttacc tgactttaa 
      aagtttcca aaagcccgt ctgtgaaca acaacaacc 
      acaacagca acaacaaaa cggtgtcgg gacctcgac 
      gaaagtagc gataccgcg tctagcctc cggtaaagc 
      tcaacaaca gcaacgatc gccccaatc gtcccccaa 
      gaagcaagt caagaatgc aaagtacaa agccatcca 
      acccaaacc gcaaccatg tgataacgc atcgtacaa 
      cccgcagac cactgcgga tatcagatc tcaaggtgt 
      actacacaa cgtagatag gtgtaagga aagaaaatc 
      aagtgatga tgattgtga tgctcccgt ttttccgct 
      gccgaggga gaggctcga aagcatcag caatgtcga 
      caaggacgc catggacga atatctagg tacaaacaa 
      cagccagca cgaagaaga gctgtcgaa atacttcag 
      caacaacat cgagacaac ggaccaaga caactccac 
      caaactctc ccaactccg tcaactcct cgaacagaa 
      cggcatatc cgacaacaa aaatttcct catcgatag 
      cggccccac cgtcgcctt gccccaccc catgctcga 
      ccctctggc cgtccccaa cgtcaacat ctatccaat 
      cccggccca gctctccac attaccgac caaccagag 
      cagcgctcg ccgtcgcgt cagcttcga aacgcccct 
      ccacgagga caccgtccc tcccagccc aaatactcg 
      ccgcaaaaa cttcagctt cacccccat atcaccggg 
      cccccaatc gcccaccgg gcgccaatc caagtgttc 
      cagcacgaa cgtaagtcc ttttgtgag tccccggaa 
      cacaccaat accccgtac taaaaacac gacccacca 
      aaacgccgg aatcggcgt ccggaaaaa aatcaaacg 
      cgaatccga tctgagcgt cgaaatccc ggacgcgaa 
      aaactacat ggccatgtc ggcccccgt cagccccat 
      gctatacaa caacaaaaa atcggtcct cgaaaaact 
      gctacattc gaacagcaa agtcgccta caccccgga 
      ttatgtcaa tccttccgt aaaacacga cgtgagcga 
      agtcgattt actcgaaag cgaaaacgt ggagacttt 
      cccggactt cacagccta taggtcgca gtcggtccc 
      tttgaacca gatgatcaa gtcgaattt caccgaaga 
      cgcccactt cctgccgac tctgcccaa tcagggcgc 
      cgatttcaa cgacttcga cccgatttc cgaatcggt 
      gaccgttaa ccggatcat tgacgccct cgacgagca 
      tccgtgcga gaatccggg cactatgga gatgtacaa 
      cgtggaaat gcctcaaaa cagccaatg cctgcccag 
      ctttaacct cattgtcga caacaacat cgacaactt 
      cggcgagaa tatggcctt tgcgcccgg atcgcgtca 
      cctggccag gtcgcaaag catcgatgt caattgttt 
      cgatatgaa atcggccaa tctcaaccc ttccagatc 
      tgtcccaag cacgcccct cccatttaa cagtagcaa 
      gcccgcccc cgtgaaaaa cttcgccaa tcagagctc 
      gcgctcgta cccgtcgac gccgtcgtt ggtggccga 
      gtcggcgtt taactacgc cgttaacgg agactgtct 
      gttgaacgg acagccgat acggaacgg aacggcgcg 
      gaatcaggc ggatgagtt gacgtattt tatgaattt 
      gaacgaaac gaacgagga caataatat cgataggca 
      tttaacggc agaggagga gtttagtat agggaggag 
      tttgccagg taatgagtt cggtattgc cgaaagtga 
      gaatgcttt gatggacga ggacggtgt cttgattgg 
      tgagcagac ttttaacgg gaatattaa taattgagg 
      aatcgtact aatgccatt tctgcggtt gcaatccgt 
      gattatgtt tattgaccc caaattttt attggtgct 
      atgataatt ggtttatca ctttgtttg tggcggtca 
      gattgtggg aaattggtc ggaacaaac gtgatcatg 
      gcgtgttat cgatatctt aatctaaag tttttaatc 
      gaaaactca tttttttgc gatgtactg aatcaaaat 
      tttatcttt ctttgagat gtattacga tatgaagta 
      atgttaatg aaatatttt tggattttc aaggtgata 
      gtttttcaa gtagtgtgc tatctcgac atcattttt 
      aacataccg ggttttacg atgtcattt tttgttttg 
      taagaaata ataattgtt attcccgtc aaattacaa 
      ataaagtag atgaaggga ctacgtaac atttttttg 
      aaaaaaatt ttaactact ttaattatg tttaattca 
      aatgtgttt aacgcattt gtatttttc tgtaaaatt 
      tcaagctag ttgttgcta aaggggcta tgtacaagc 
      tattataaa atttagttg tggatgaag gggttgtgt 
      ccaattaat ttgtattgc ataataatt ttttttttg 
      ttggtaaca ttttaagcc ttctttttt atataagaa 
      ttctaaatt ataaacact actttaaaa cacgttttc 
      ggtagcaac acttctgcg aaaacgctt aaagttctc 
      gggtctcca ggtatgcaa ctcgcatac gctcgttgc 
      ataacgaca gccctcgaa ctttaactt tagaccctt 
      catccatta acccttgca aaaattaaa aaaaataaa 
      ccttttgat aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY