General information | |||
TC008959 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 12709493..12716100 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC008959 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04568 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC008959 RA
MMETQQWPA KFQQSGAGT DTTQDSLNN GEDGNVAKE ENGDGVHES DKRAENDSP TISEKARQL ISLILEQMK ELSNAEKYL LYLKLFQIS NVVDPLRQP LNPLGSRSE INRTISWIK THLEEDAAL SLPKQEVYE EYTVYCTQN QIKSLSQAD FGKVMKQVY PKVRARRLG TRGNSRYCY SGLRRCIKL KPPKLPDLG DKPLSTEAP CTQSTLTAA AWLIVKEWS EFHFGVQFP SLQALARFL VVSHSVGAG TDAANQLTS ATESQLKGE VCGGKTGTK HREMQLQLQ KKIQQKNEV KERKRKIQS PKSDPKPTV KKSKGSVSS IRLEGSPTL TGAGESSTS TSTGSTSTS PTQGKSICD KSLDFTQLP ALPDFKSFQ KPVCEQQQP QQQQQNGVG TSTKVAIPR LASGKAQQQ QRSPQSSPK KQVKNAKYK AIQPKPQPC DNASYNPQT TADIRSQGV LHNVDRCKE RKSSDDDCD APVFPLPRE RLESISNVD KDAMDEYLG TNNSQHEEE LSKYFSNNI ETTDQDNST KLSQLRQLL EQNGISDNK NFLIDSGPT VALPHPMLD PLAVPNVNI YPIPAQLST LPTNQSSAR RRVSFETPL HEDTVPPSP NTRRKNFSF TPISPGPQS PTGRQSKCS STNVSPFVS PRNTPIPRT KNTTHQNAG IGVRKKIKR ESDLSVEIP DAKNYMAMS APVSPMLYN NKKSVLEKL LHSNSKVAY TPDYVNPSV KHDVSEVDL LESENVETF PDFTAYRSQ SVPLNQMIK SNFTEDAHF LPTLPNQGA DFNDFDPIS ESVTVNRII DALDEHPCE NPGTMEMYN VEMPQNSQC LPSFNLIVD NNIDNFGEN MAFAPGSRH LARSQSIDV NCFDMKSAN LNPSRSVPS TPLPFNSSK PAPVKNFAN QSSRSYPST PSLVAESAF NYAVNGDCL LNGQPIRNG TARNQADEL TYFMNLNET NEDNNIDRH LTAEEEFSI GRSLPGNEF GIAESENAL MDEDGVLIG EQTFNGNIN N
- >TC008959 RA
atgatggaa actcaacaa tggcccgca aaattccaa caatctggg gccgggacc gacaccaca caagatagt ttaaacaat ggagaggac ggcaacgtc gcgaaagaa gaaaacggc gatggggtg cacgagagc gacaagcgg gccgaaaac gacagcccc acaatcagt gagaaagcc agacagtta atatcgttg atcttagag cagatgaaa gaattgtca aacgccgaa aaatatctc ttgtatttg aaactgttt caaatcagc aatgtagtg gaccctttg cggcaaccc ttgaaccca ctgggcagt cgttccgaa atcaacagg accatatca tggattaag actcactta gaagaagac gcagcgctt tctctcccc aagcaggag gtctacgag gaatataca gtatattgc acacaaaat caaatcaag tctctttcc caagctgat tttggcaag gtcatgaag caagtgtat ccaaaagtg agggctagg cgcttgggt acccgcgga aattccaga tattgctat tcaggcttg agaagatgt atcaagtta aagcctcca aaattgcca gatcttggc gataagcct ttaagcacg gaagcccct tgtactcag tcgacttta acagcggcg gcttggtta attgtgaaa gaatggtct gaatttcat tttggagtt cagtttcca tcattgcaa gccttggcc cggtttctt gttgtttct cattcagtt ggtgcagga actgacgct gccaaccag ttgacttca gctacagaa tcacagttg aaaggggag gtgtgtggt gggaaaact ggaaccaaa cacagagaa atgcagctg cagttgcag aagaagatc caacagaag aacgaagtc aaagaaaga aaaagaaaa atacagtct cccaaatcc gatccgaag cccactgtg aagaaatca aagggatca gtgtcgtcg atccgactc gaaggttcc cccacattg acgggtgcg ggtgaaagc agcaccagt acaagcacc ggcagcacc agcacgagt cccactcag ggcaaatct atctgtgat aaatcgtta gatttcact caactacca gcgttacct gactttaaa agtttccaa aagcccgtc tgtgaacaa caacaacca caacagcaa caacaaaac ggtgtcggg acctcgacg aaagtagcg ataccgcgt ctagcctcc ggtaaagct caacaacag caacgatcg ccccaatcg tcccccaag aagcaagtc aagaatgca aagtacaaa gccatccaa cccaaaccg caaccatgt gataacgca tcgtacaac ccgcagacc actgcggat atcagatct caaggtgta ctacacaac gtagatagg tgtaaggaa agaaaatca agtgatgat gattgtgat gctcccgtt tttccgctg ccgagggag aggctcgaa agcatcagc aatgtcgac aaggacgcc atggacgaa tatctaggt acaaacaac agccagcac gaagaagag ctgtcgaaa tacttcagc aacaacatc gagacaacg gaccaagac aactccacc aaactctcc caactccgt caactcctc gaacagaac ggcatatcc gacaacaaa aatttcctc atcgatagc ggccccacc gtcgccttg ccccacccc atgctcgac cctctggcc gtccccaac gtcaacatc tatccaatc ccggcccag ctctccaca ttaccgacc aaccagagc agcgctcgc cgtcgcgtc agcttcgaa acgcccctc cacgaggac accgtccct cccagccca aatactcgc cgcaaaaac ttcagcttc acccccata tcaccgggc ccccaatcg cccaccggg cgccaatcc aagtgttcc agcacgaac gtaagtcct tttgtgagt ccccggaac acaccaata ccccgtact aaaaacacg acccaccaa aacgccgga atcggcgtc cggaaaaaa atcaaacgc gaatccgat ctgagcgtc gaaatcccg gacgcgaaa aactacatg gccatgtcg gcccccgtc agccccatg ctatacaac aacaaaaaa tcggtcctc gaaaaactg ctacattcg aacagcaaa gtcgcctac accccggat tatgtcaat ccttccgta aaacacgac gtgagcgaa gtcgattta ctcgaaagc gaaaacgtg gagactttc ccggacttc acagcctat aggtcgcag tcggtccct ttgaaccag atgatcaag tcgaatttc accgaagac gcccacttc ctgccgact ctgcccaat cagggcgcc gatttcaac gacttcgac ccgatttcc gaatcggtg accgttaac cggatcatt gacgccctc gacgagcat ccgtgcgag aatccgggc actatggag atgtacaac gtggaaatg cctcaaaac agccaatgc ctgcccagc tttaacctc attgtcgac aacaacatc gacaacttc ggcgagaat atggccttt gcgcccgga tcgcgtcac ctggccagg tcgcaaagc atcgatgtc aattgtttc gatatgaaa tcggccaat ctcaaccct tccagatct gtcccaagc acgcccctc ccatttaac agtagcaag cccgccccc gtgaaaaac ttcgccaat cagagctcg cgctcgtac ccgtcgacg ccgtcgttg gtggccgag tcggcgttt aactacgcc gttaacgga gactgtctg ttgaacgga cagccgata cggaacgga acggcgcgg aatcaggcg gatgagttg acgtatttt atgaatttg aacgaaacg aacgaggac aataatatc gataggcat ttaacggca gaggaggag tttagtata gggaggagt ttgccaggt aatgagttc ggtattgcc gaaagtgag aatgctttg atggacgag gacggtgtc ttgattggt gagcagact tttaacggg aatattaat aattga
- >TC008959 RA
atggataaa aaacgttta gaagaacga aaggattgt cgtccattg gagaccagc tgatttgag ttgacaata aaatgttcc ggtcttgtc agcccgtat tggctggta aagtgtcga aaaattgtg cggttcgtg ctgaaacca actgaaata ggccaaaaa attgctggc gatgatgga aactcaaca atggcccgc aaaattcca acaatctgg ggccgggac cgacaccac acaagatag tttaaacaa tggagagga cggcaacgt cgcgaaaga agaaaacgg cgatggggt gcacgagag cgacaagcg ggccgaaaa cgacagccc cacaatcag tgagaaagc cagacagtt aatatcgtt gatcttaga gcagatgaa agaattgtc aaacgccga aaaatatct cttgtattt gaaactgtt tcaaatcag caatgtagt ggacccttt gcggcaacc cttgaaccc actgggcag tcgttccga aatcaacag gaccatatc atggattaa gactcactt agaagaaga cgcagcgct ttctctccc caagcagga ggtctacga ggaatatac agtatattg cacacaaaa tcaaatcaa gtctctttc ccaagctga ttttggcaa ggtcatgaa gcaagtgta tccaaaagt gagggctag gcgcttggg tacccgcgg aaattccag atattgcta ttcaggctt gagaagatg tatcaagtt aaagcctcc aaaattgcc agatcttgg cgataagcc tttaagcac ggaagcccc ttgtactca gtcgacttt aacagcggc ggcttggtt aattgtgaa agaatggtc tgaatttca ttttggagt tcagtttcc atcattgca agccttggc ccggtttct tgttgtttc tcattcagt tggtgcagg aactgacgc tgccaacca gttgacttc agctacaga atcacagtt gaaagggga ggtgtgtgg tgggaaaac tggaaccaa acacagaga aatgcagct gcagttgca gaagaagat ccaacagaa gaacgaagt caaagaaag aaaaagaaa aatacagtc tcccaaatc cgatccgaa gcccactgt gaagaaatc aaagggatc agtgtcgtc gatccgact cgaaggttc ccccacatt gacgggtgc gggtgaaag cagcaccag tacaagcac cggcagcac cagcacgag tcccactca gggcaaatc tatctgtga taaatcgtt agatttcac tcaactacc agcgttacc tgactttaa aagtttcca aaagcccgt ctgtgaaca acaacaacc acaacagca acaacaaaa cggtgtcgg gacctcgac gaaagtagc gataccgcg tctagcctc cggtaaagc tcaacaaca gcaacgatc gccccaatc gtcccccaa gaagcaagt caagaatgc aaagtacaa agccatcca acccaaacc gcaaccatg tgataacgc atcgtacaa cccgcagac cactgcgga tatcagatc tcaaggtgt actacacaa cgtagatag gtgtaagga aagaaaatc aagtgatga tgattgtga tgctcccgt ttttccgct gccgaggga gaggctcga aagcatcag caatgtcga caaggacgc catggacga atatctagg tacaaacaa cagccagca cgaagaaga gctgtcgaa atacttcag caacaacat cgagacaac ggaccaaga caactccac caaactctc ccaactccg tcaactcct cgaacagaa cggcatatc cgacaacaa aaatttcct catcgatag cggccccac cgtcgcctt gccccaccc catgctcga ccctctggc cgtccccaa cgtcaacat ctatccaat cccggccca gctctccac attaccgac caaccagag cagcgctcg ccgtcgcgt cagcttcga aacgcccct ccacgagga caccgtccc tcccagccc aaatactcg ccgcaaaaa cttcagctt cacccccat atcaccggg cccccaatc gcccaccgg gcgccaatc caagtgttc cagcacgaa cgtaagtcc ttttgtgag tccccggaa cacaccaat accccgtac taaaaacac gacccacca aaacgccgg aatcggcgt ccggaaaaa aatcaaacg cgaatccga tctgagcgt cgaaatccc ggacgcgaa aaactacat ggccatgtc ggcccccgt cagccccat gctatacaa caacaaaaa atcggtcct cgaaaaact gctacattc gaacagcaa agtcgccta caccccgga ttatgtcaa tccttccgt aaaacacga cgtgagcga agtcgattt actcgaaag cgaaaacgt ggagacttt cccggactt cacagccta taggtcgca gtcggtccc tttgaacca gatgatcaa gtcgaattt caccgaaga cgcccactt cctgccgac tctgcccaa tcagggcgc cgatttcaa cgacttcga cccgatttc cgaatcggt gaccgttaa ccggatcat tgacgccct cgacgagca tccgtgcga gaatccggg cactatgga gatgtacaa cgtggaaat gcctcaaaa cagccaatg cctgcccag ctttaacct cattgtcga caacaacat cgacaactt cggcgagaa tatggcctt tgcgcccgg atcgcgtca cctggccag gtcgcaaag catcgatgt caattgttt cgatatgaa atcggccaa tctcaaccc ttccagatc tgtcccaag cacgcccct cccatttaa cagtagcaa gcccgcccc cgtgaaaaa cttcgccaa tcagagctc gcgctcgta cccgtcgac gccgtcgtt ggtggccga gtcggcgtt taactacgc cgttaacgg agactgtct gttgaacgg acagccgat acggaacgg aacggcgcg gaatcaggc ggatgagtt gacgtattt tatgaattt gaacgaaac gaacgagga caataatat cgataggca tttaacggc agaggagga gtttagtat agggaggag tttgccagg taatgagtt cggtattgc cgaaagtga gaatgcttt gatggacga ggacggtgt cttgattgg tgagcagac ttttaacgg gaatattaa taattgagg aatcgtact aatgccatt tctgcggtt gcaatccgt gattatgtt tattgaccc caaattttt attggtgct atgataatt ggtttatca ctttgtttg tggcggtca gattgtggg aaattggtc ggaacaaac gtgatcatg gcgtgttat cgatatctt aatctaaag tttttaatc gaaaactca tttttttgc gatgtactg aatcaaaat tttatcttt ctttgagat gtattacga tatgaagta atgttaatg aaatatttt tggattttc aaggtgata gtttttcaa gtagtgtgc tatctcgac atcattttt aacataccg ggttttacg atgtcattt tttgttttg taagaaata ataattgtt attcccgtc aaattacaa ataaagtag atgaaggga ctacgtaac atttttttg aaaaaaatt ttaactact ttaattatg tttaattca aatgtgttt aacgcattt gtatttttc tgtaaaatt tcaagctag ttgttgcta aaggggcta tgtacaagc tattataaa atttagttg tggatgaag gggttgtgt ccaattaat ttgtattgc ataataatt ttttttttg ttggtaaca ttttaagcc ttctttttt atataagaa ttctaaatt ataaacact actttaaaa cacgttttc ggtagcaac acttctgcg aaaacgctt aaagttctc gggtctcca ggtatgcaa ctcgcatac gctcgttgc ataacgaca gccctcgaa ctttaactt tagaccctt catccatta acccttgca aaaattaaa aaaaataaa ccttttgat aaaac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
