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Gene information
General information
TC008926 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11981516..11988157
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008926
    iBeetle screen iB_04564

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs sometimes fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partially duplicated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen partially duplicated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008926 RA
      MSQSQEEMR PRAVYSIDQ ILGVNSSST SAKSIEGES 
      DSKVDSVSD SEMVEESIE DLNDTRPRK IRRSRTTFT 
      TYQLHQLER AFEKTQYPD VFTREELAM RLDLSEARV 
      QVWFQNRRA KWRKREKAL GREAVPFMH PGDQPGLPE 
      FSLHGHMAM PPVGPADSF WPSLPFPPM FSPAIGLPW 
      PPKTPMNVP TFHAFLSQY VLAGGVPQL NLLNSGIPD 
      ERSRSTSPE TPSPSQLSP SALEALRLR TQEILVPSS 
      SPQKLHAKS
    CDS sequence
    • >TC008926 RA
      atgtcgcaa agtcaagaa gaaatgcgt ccgagggcc 
      gtctacagc atcgaccag attttggga gttaacagc 
      tcgtccacc tctgcgaag agcatcgaa ggcgagtcg 
      gattccaaa gtcgattcg gtcagtgat agcgaaatg 
      gttgaggaa agcatcgaa gatttgaac gacaccaga 
      ccgagaaaa atccgcaga tcccgaacg actttcacc 
      acctatcag ctgcaccaa ctggagcga gctttcgaa 
      aaaacccaa taccccgac gttttcaca agagaggaa 
      ttggctatg cggcttgat ttgagcgaa gcgcgagtc 
      caggtatgg ttccaaaac cgtcgcgcc aagtggaga 
      aagcgtgaa aaggcctta ggccgggaa gccgtcccg 
      ttcatgcac cctggggac cagcccggt ctgcccgaa 
      ttctcgctg catgggcac atggcgatg cccccggtg 
      ggccccgcc gactccttc tggcccagt ttgcctttc 
      ccgcccatg ttcagtccg gccataggc ctgccttgg 
      ccgcccaag acgcccatg aacgtgccg acgttccac 
      gctttcctg tcgcaatac gtgctcgca ggcggcgtc 
      ccccaactg aatctccta aatagtggg attccggac 
      gaacggtcc cggagtacg agtccggaa actcccagt 
      ccgtcgcag ttgtcgccc tcagcgctg gaagctttg 
      aggttacgg acacaagaa attttagtg ccgagttct 
      tcgccgcag aaactgcac gcgaagtcg taa
    mRNA sequence
    • >TC008926 RA
      tcagtcgag aagcaagtt ttacgccga tcgtttcaa 
      tacaaaacg tgcttatac ctcttcagt gttctgttg 
      acatgtcgc aaagtcaag aagaaatgc gtccgaggg 
      ccgtctaca gcatcgacc agattttgg gagttaaca 
      gctcgtcca cctctgcga agagcatcg aaggcgagt 
      cggattcca aagtcgatt cggtcagtg atagcgaaa 
      tggttgagg aaagcatcg aagatttga acgacacca 
      gaccgagaa aaatccgca gatcccgaa cgactttca 
      ccacctatc agctgcacc aactggagc gagctttcg 
      aaaaaaccc aataccccg acgttttca caagagagg 
      aattggcta tgcggcttg atttgagcg aagcgcgag 
      tccaggtat ggttccaaa accgtcgcg ccaagtgga 
      gaaagcgtg aaaaggcct taggccggg aagccgtcc 
      cgttcatgc accctgggg accagcccg gtctgcccg 
      aattctcgc tgcatgggc acatggcga tgcccccgg 
      tgggccccg ccgactcct tctggccca gtttgcctt 
      tcccgccca tgttcagtc cggccatag gcctgcctt 
      ggccgccca agacgccca tgaacgtgc cgacgttcc 
      acgctttcc tgtcgcaat acgtgctcg caggcggcg 
      tcccccaac tgaatctcc taaatagtg ggattccgg 
      acgaacggt cccggagta cgagtccgg aaactccca 
      gtccgtcgc agttgtcgc cctcagcgc tggaagctt 
      tgaggttac ggacacaag aaattttag tgccgagtt 
      cttcgccgc agaaactgc acgcgaagt cgtaaatag 
      ataatatct agtggacat gtagttgta ttgttaggg 
      atgtaggag attattggc gcttgatat gtgggggaa 
      atagactga atagctgta ccaagtttt cgatttagt 
      tgttatttt tttttcatt cttatgata gggagaatt 
      atttttgta ttaaattta tttacaaca ctagttgca 
      ctagttagt ttagcgcca attttgtaa ataattttg 
      tacataaag tgcgtattt atttattgt agataaaaa 
      atcaagagg tttgtttaa aacataata gcattacag 
      ataattatt ttgtggggg aaattaaca ataagttgt 
      acctaatac ctagttacg atcgatatt aaacgaaac 
      aataattaa aacggcagg tgttttact tgtcttggt 
      tatcaaaag tttattatc aataagtaa taatctact 
      taattatgg cgtgtttat tggttcatc taccttgaa 
      tgcttgtaa aataaccat tattacatt gatgtttac 
      atagaaatg attgataat attataaaa ttgtaataa 
      taacatttt aacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY