Status
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Gene information
General information
TC008920 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11908666..11911428
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008920
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04563

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008920 RA
      MSFGTEEYY KRFIELQEK LRKSEEERL KLEMKFNEM 
      VQVTREEEQ MHYRKLRAQ YKRFLEEDR RRQDRNERI 
      LRSLERIES RVAMLVAKT ERFKLLRQQ YQNFLDRIY 
      RTKQAKIED VKPKEVEKP KTDDILQSY IQNLSSKKL 
      REMLRKEED ETFDSGHPF EGDYRPNYA NSIADDIMS 
      SIYSKPSKQ HPREFNNNN IWGAKMRAK SSTEDGSDI 
      DEIPYNSGV SEAVKNFVR MGKRDSLVE QPTKIDHSL 
      PRIEKEAEE TDQNIKIEE KLNTVDLIE PQNNFESLR 
      NKRDVSKVE STLQPEQQV QQQIDEVDS KVSTQVQPE 
      QTKEGIEEK QQVQVAVDE SGQQNVERY TDQVQHYNL 
      EQKHVDQGQ FEQQSTEQT QFIQQHVDQ GEFEQQQYT 
      DQGQFEQQH YANNSNPKT EQQYEKIEE PILPTQQYD 
      DQAQFVQQF NKTEQPVQE QHFESEQPI PSQQFDDQG 
      QYDENGQLI QEQQYDGEP QIPTQQYDD QGQFVQQYD 
      TSEQQLYDQ SGQQYDENG QPVQQITEQ GQFVQQYDE 
      SGQHIQQYD EQYGQQYDQ HGQYLQQYD ETGQPIQQY 
      DESGQPLQQ YDESGQPIQ QYDGTGQSI QQYDEQPVQ 
      QYDESGQPI QQYDENGQP IQQYDETGQ AIQQYGENG 
      QPIQQYDET GQYGQHYDE NGQPTQQFD DNGQPIQQY 
      DENAQYYGD QSNVQYDEN GQMIQHYDQ PYDGVVEHQ 
      NEVEGDDQS VPAPSEPKK SNILELLDT DTESMKQES 
      KVSHDSDFD FSNE
    CDS sequence
    • >TC008920 RA
      atgtcgttt gggaccgag gagtactac aagcgattc 
      atcgaattg caggaaaag ctccgcaag agcgaggag 
      gaacgcctc aaacttgaa atgaaattt aacgaaatg 
      gttcaagtt acacgagag gaggaacaa atgcattat 
      cggaaattg cgggctcaa tacaagcgg ttcttggag 
      gaggacagg cggagacag gaccgaaat gagcgcatt 
      ttacgcagt ttggagagg attgaaagt cgggttgcg 
      atgctagtg gcgaagact gaacgattt aaactgttg 
      agacagcag taccaaaat tttttggac cggatttat 
      cgcaccaaa caagcaaaa attgaagat gtcaagccc 
      aaagaagtc gagaaaccc aaaactgac gatattttg 
      cagagttac atacaaaat ttgtcgtcg aagaaattg 
      agggaaatg ttgaggaag gaggaagat gagactttc 
      gattcgggt caccccttt gagggcgat tatagaccg 
      aattatgcg aattccatc gccgatgat atcatgagt 
      tcgatttat agcaaaccc agtaaacag caccctcga 
      gagtttaat aataataat atttggggc gcgaaaatg 
      cgggccaag agtagtact gaggacggt tccgacatt 
      gacgaaatt ccgtacaac agtggtgtt agtgaagcc 
      gtgaaaaat ttcgttcga atggggaaa agggacagt 
      ttggttgaa caaccaacg aaaattgat cattccttg 
      ccgcgaatt gagaaggag gctgaagaa actgatcaa 
      aacataaaa attgaagaa aaactgaac accgtggat 
      ttgattgaa ccacaaaat aattttgaa agtctaaga 
      aataaaaga gatgtttcg aaagttgaa agtacactt 
      caacccgaa caacaagta caacaacaa attgatgaa 
      gttgatagc aaagtttca acacaagtt caacctgaa 
      caaacaaag gaaggtatt gaagagaaa caacaagta 
      caggtcgct gtggatgaa agtgggcag caaaacgtt 
      gaacgctac actgatcaa gttcaacat tataatctt 
      gaacaaaaa catgtggat caaggacaa tttgaacaa 
      cagtccact gaacaaact cagtttatc caacaacat 
      gtggatcaa ggcgaattt gaacaacaa caatacact 
      gaccaaggt caatttgaa cagcagcac tatgccaat 
      aattctaat ccgaaaact gaacaacag tatgaaaaa 
      attgaagaa ccaattctt ccaacgcaa caatatgat 
      gatcaagcc cagtttgta caacagttt aataaaact 
      gagcaacct gttcaagaa caacatttt gaaagtgag 
      cagccaatc ccgtcacaa cagtttgac gatcaaggc 
      caatacgat gaaaatgga caactgatt caagaacaa 
      caatatgat ggtgaacca caaatacca acgcaacaa 
      tatgacgat caaggtcaa tttgtgcaa caatatgat 
      actagtgaa cagcagttg tacgatcag agtggacaa 
      cagtatgat gaaaacgga caacctgtg caacagatt 
      actgaacaa ggtcaattt gtgcaacag tacgacgaa 
      agtggacaa catattcaa cagtacgat gaacaatac 
      ggacaacag tacgatcaa cacggccag tacctacaa 
      caatatgac gaaaccgga caacctatt caacaatat 
      gatgaaagt gggcaacct cttcaacaa tacgacgaa 
      agtggacaa cctattcaa cagtatgac ggaactggg 
      cagtctatt caacagtat gatgaacaa cctgttcaa 
      caatacgac gaaagtgga caacctatt caacagtac 
      gacgaaaat ggacaacct attcaacag tacgacgaa 
      actggacag gctattcaa cagtatggt gaaaatgga 
      caacctatt caacagtat gacgaaact ggacaatat 
      gggcaacat tatgacgaa aacggacaa ccgactcaa 
      cagtttgat gataatgga caacctatt caacaatat 
      gacgaaaat gcgcaatac tatggcgat caaagtaat 
      gtgcaatat gatgaaaac ggtcaaatg attcagcat 
      tatgaccaa ccgtatgat ggagtggtt gaacatcag 
      aatgaagtc gaaggggac gatcagagt gttcctgca 
      ccgtcagaa ccaaagaaa agtaacatt ttggaactg 
      ttagatact gacactgaa agtatgaag caggagtcg 
      aaagtttca cacgatagt gattttgat ttcagtaat 
      gaataa
    mRNA sequence
    • >TC008920 RA
      gtcgaactc cacccctag tggctttgt ttcgcccaa 
      ttgtcttgt gattgcgat aacgagtga ttaattgaa 
      atcggttca cacgcgaag ctttgatta aaacccgca 
      attcgattg attcggtga aataaaatt gttgcattc 
      gcctgtcgt gcgccaagt aattacgtc ccacatgca 
      taatgtggt gcgttgctg gctagacca ctgcaaaac 
      tagcttcaa attcgcgta attgtcaaa aaaaaaaac 
      tgcgaaatg tcgtttggg accgaggag tactacaag 
      cgattcatc gaattgcag gaaaagctc cgcaagagc 
      gaggaggaa cgcctcaaa cttgaaatg aaatttaac 
      gaaatggtt caagttaca cgagaggag gaacaaatg 
      cattatcgg aaattgcgg gctcaatac aagcggttc 
      ttggaggag gacaggcgg agacaggac cgaaatgag 
      cgcatttta cgcagtttg gagaggatt gaaagtcgg 
      gttgcgatg ctagtggcg aagactgaa cgatttaaa 
      ctgttgaga cagcagtac caaaatttt ttggaccgg 
      atttatcgc accaaacaa gcaaaaatt gaagatgtc 
      aagcccaaa gaagtcgag aaacccaaa actgacgat 
      attttgcag agttacata caaaatttg tcgtcgaag 
      aaattgagg gaaatgttg aggaaggag gaagatgag 
      actttcgat tcgggtcac ccctttgag ggcgattat 
      agaccgaat tatgcgaat tccatcgcc gatgatatc 
      atgagttcg atttatagc aaacccagt aaacagcac 
      cctcgagag tttaataat aataatatt tggggcgcg 
      aaaatgcgg gccaagagt agtactgag gacggttcc 
      gacattgac gaaattccg tacaacagt ggtgttagt 
      gaagccgtg aaaaatttc gttcgaatg gggaaaagg 
      gacagtttg gttgaacaa ccaacgaaa attgatcat 
      tccttgccg cgaattgag aaggaggct gaagaaact 
      gatcaaaac ataaaaatt gaagaaaaa ctgaacacc 
      gtggatttg attgaacca caaaataat tttgaaagt 
      ctaagaaat aaaagagat gtttcgaaa gttgaaagt 
      acacttcaa cccgaacaa caagtacaa caacaaatt 
      gatgaagtt gatagcaaa gtttcaaca caagttcaa 
      cctgaacaa acaaaggaa ggtattgaa gagaaacaa 
      caagtacag gtcgctgtg gatgaaagt gggcagcaa 
      aacgttgaa cgctacact gatcaagtt caacattat 
      aatcttgaa caaaaacat gtggatcaa ggacaattt 
      gaacaacag tccactgaa caaactcag tttatccaa 
      caacatgtg gatcaaggc gaatttgaa caacaacaa 
      tacactgac caaggtcaa tttgaacag cagcactat 
      gccaataat tctaatccg aaaactgaa caacagtat 
      gaaaaaatt gaagaacca attcttcca acgcaacaa 
      tatgatgat caagcccag tttgtacaa cagtttaat 
      aaaactgag caacctgtt caagaacaa cattttgaa 
      agtgagcag ccaatcccg tcacaacag tttgacgat 
      caaggccaa tacgatgaa aatggacaa ctgattcaa 
      gaacaacaa tatgatggt gaaccacaa ataccaacg 
      caacaatat gacgatcaa ggtcaattt gtgcaacaa 
      tatgatact agtgaacag cagttgtac gatcagagt 
      ggacaacag tatgatgaa aacggacaa cctgtgcaa 
      cagattact gaacaaggt caatttgtg caacagtac 
      gacgaaagt ggacaacat attcaacag tacgatgaa 
      caatacgga caacagtac gatcaacac ggccagtac 
      ctacaacaa tatgacgaa accggacaa cctattcaa 
      caatatgat gaaagtggg caacctctt caacaatac 
      gacgaaagt ggacaacct attcaacag tatgacgga 
      actgggcag tctattcaa cagtatgat gaacaacct 
      gttcaacaa tacgacgaa agtggacaa cctattcaa 
      cagtacgac gaaaatgga caacctatt caacagtac 
      gacgaaact ggacaggct attcaacag tatggtgaa 
      aatggacaa cctattcaa cagtatgac gaaactgga 
      caatatggg caacattat gacgaaaac ggacaaccg 
      actcaacag tttgatgat aatggacaa cctattcaa 
      caatatgac gaaaatgcg caatactat ggcgatcaa 
      agtaatgtg caatatgat gaaaacggt caaatgatt 
      cagcattat gaccaaccg tatgatgga gtggttgaa 
      catcagaat gaagtcgaa ggggacgat cagagtgtt 
      cctgcaccg tcagaacca aagaaaagt aacattttg 
      gaactgtta gatactgac actgaaagt atgaagcag 
      gagtcgaaa gtttcacac gatagtgat tttgatttc 
      agtaatgaa taagtttgg tagtttagc catagaaat 
      gtaatgaat gacacactt gatttaggt ctgtgattc 
      agttttttt aatacaaca ctaataaaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY