| General information | |||
| TC008920 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 11908666..11911428 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008920 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04563 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC008920 RA
MSFGTEEYY KRFIELQEK LRKSEEERL KLEMKFNEM VQVTREEEQ MHYRKLRAQ YKRFLEEDR RRQDRNERI LRSLERIES RVAMLVAKT ERFKLLRQQ YQNFLDRIY RTKQAKIED VKPKEVEKP KTDDILQSY IQNLSSKKL REMLRKEED ETFDSGHPF EGDYRPNYA NSIADDIMS SIYSKPSKQ HPREFNNNN IWGAKMRAK SSTEDGSDI DEIPYNSGV SEAVKNFVR MGKRDSLVE QPTKIDHSL PRIEKEAEE TDQNIKIEE KLNTVDLIE PQNNFESLR NKRDVSKVE STLQPEQQV QQQIDEVDS KVSTQVQPE QTKEGIEEK QQVQVAVDE SGQQNVERY TDQVQHYNL EQKHVDQGQ FEQQSTEQT QFIQQHVDQ GEFEQQQYT DQGQFEQQH YANNSNPKT EQQYEKIEE PILPTQQYD DQAQFVQQF NKTEQPVQE QHFESEQPI PSQQFDDQG QYDENGQLI QEQQYDGEP QIPTQQYDD QGQFVQQYD TSEQQLYDQ SGQQYDENG QPVQQITEQ GQFVQQYDE SGQHIQQYD EQYGQQYDQ HGQYLQQYD ETGQPIQQY DESGQPLQQ YDESGQPIQ QYDGTGQSI QQYDEQPVQ QYDESGQPI QQYDENGQP IQQYDETGQ AIQQYGENG QPIQQYDET GQYGQHYDE NGQPTQQFD DNGQPIQQY DENAQYYGD QSNVQYDEN GQMIQHYDQ PYDGVVEHQ NEVEGDDQS VPAPSEPKK SNILELLDT DTESMKQES KVSHDSDFD FSNE
- >TC008920 RA
atgtcgttt gggaccgag gagtactac aagcgattc atcgaattg caggaaaag ctccgcaag agcgaggag gaacgcctc aaacttgaa atgaaattt aacgaaatg gttcaagtt acacgagag gaggaacaa atgcattat cggaaattg cgggctcaa tacaagcgg ttcttggag gaggacagg cggagacag gaccgaaat gagcgcatt ttacgcagt ttggagagg attgaaagt cgggttgcg atgctagtg gcgaagact gaacgattt aaactgttg agacagcag taccaaaat tttttggac cggatttat cgcaccaaa caagcaaaa attgaagat gtcaagccc aaagaagtc gagaaaccc aaaactgac gatattttg cagagttac atacaaaat ttgtcgtcg aagaaattg agggaaatg ttgaggaag gaggaagat gagactttc gattcgggt caccccttt gagggcgat tatagaccg aattatgcg aattccatc gccgatgat atcatgagt tcgatttat agcaaaccc agtaaacag caccctcga gagtttaat aataataat atttggggc gcgaaaatg cgggccaag agtagtact gaggacggt tccgacatt gacgaaatt ccgtacaac agtggtgtt agtgaagcc gtgaaaaat ttcgttcga atggggaaa agggacagt ttggttgaa caaccaacg aaaattgat cattccttg ccgcgaatt gagaaggag gctgaagaa actgatcaa aacataaaa attgaagaa aaactgaac accgtggat ttgattgaa ccacaaaat aattttgaa agtctaaga aataaaaga gatgtttcg aaagttgaa agtacactt caacccgaa caacaagta caacaacaa attgatgaa gttgatagc aaagtttca acacaagtt caacctgaa caaacaaag gaaggtatt gaagagaaa caacaagta caggtcgct gtggatgaa agtgggcag caaaacgtt gaacgctac actgatcaa gttcaacat tataatctt gaacaaaaa catgtggat caaggacaa tttgaacaa cagtccact gaacaaact cagtttatc caacaacat gtggatcaa ggcgaattt gaacaacaa caatacact gaccaaggt caatttgaa cagcagcac tatgccaat aattctaat ccgaaaact gaacaacag tatgaaaaa attgaagaa ccaattctt ccaacgcaa caatatgat gatcaagcc cagtttgta caacagttt aataaaact gagcaacct gttcaagaa caacatttt gaaagtgag cagccaatc ccgtcacaa cagtttgac gatcaaggc caatacgat gaaaatgga caactgatt caagaacaa caatatgat ggtgaacca caaatacca acgcaacaa tatgacgat caaggtcaa tttgtgcaa caatatgat actagtgaa cagcagttg tacgatcag agtggacaa cagtatgat gaaaacgga caacctgtg caacagatt actgaacaa ggtcaattt gtgcaacag tacgacgaa agtggacaa catattcaa cagtacgat gaacaatac ggacaacag tacgatcaa cacggccag tacctacaa caatatgac gaaaccgga caacctatt caacaatat gatgaaagt gggcaacct cttcaacaa tacgacgaa agtggacaa cctattcaa cagtatgac ggaactggg cagtctatt caacagtat gatgaacaa cctgttcaa caatacgac gaaagtgga caacctatt caacagtac gacgaaaat ggacaacct attcaacag tacgacgaa actggacag gctattcaa cagtatggt gaaaatgga caacctatt caacagtat gacgaaact ggacaatat gggcaacat tatgacgaa aacggacaa ccgactcaa cagtttgat gataatgga caacctatt caacaatat gacgaaaat gcgcaatac tatggcgat caaagtaat gtgcaatat gatgaaaac ggtcaaatg attcagcat tatgaccaa ccgtatgat ggagtggtt gaacatcag aatgaagtc gaaggggac gatcagagt gttcctgca ccgtcagaa ccaaagaaa agtaacatt ttggaactg ttagatact gacactgaa agtatgaag caggagtcg aaagtttca cacgatagt gattttgat ttcagtaat gaataa
- >TC008920 RA
gtcgaactc cacccctag tggctttgt ttcgcccaa ttgtcttgt gattgcgat aacgagtga ttaattgaa atcggttca cacgcgaag ctttgatta aaacccgca attcgattg attcggtga aataaaatt gttgcattc gcctgtcgt gcgccaagt aattacgtc ccacatgca taatgtggt gcgttgctg gctagacca ctgcaaaac tagcttcaa attcgcgta attgtcaaa aaaaaaaac tgcgaaatg tcgtttggg accgaggag tactacaag cgattcatc gaattgcag gaaaagctc cgcaagagc gaggaggaa cgcctcaaa cttgaaatg aaatttaac gaaatggtt caagttaca cgagaggag gaacaaatg cattatcgg aaattgcgg gctcaatac aagcggttc ttggaggag gacaggcgg agacaggac cgaaatgag cgcatttta cgcagtttg gagaggatt gaaagtcgg gttgcgatg ctagtggcg aagactgaa cgatttaaa ctgttgaga cagcagtac caaaatttt ttggaccgg atttatcgc accaaacaa gcaaaaatt gaagatgtc aagcccaaa gaagtcgag aaacccaaa actgacgat attttgcag agttacata caaaatttg tcgtcgaag aaattgagg gaaatgttg aggaaggag gaagatgag actttcgat tcgggtcac ccctttgag ggcgattat agaccgaat tatgcgaat tccatcgcc gatgatatc atgagttcg atttatagc aaacccagt aaacagcac cctcgagag tttaataat aataatatt tggggcgcg aaaatgcgg gccaagagt agtactgag gacggttcc gacattgac gaaattccg tacaacagt ggtgttagt gaagccgtg aaaaatttc gttcgaatg gggaaaagg gacagtttg gttgaacaa ccaacgaaa attgatcat tccttgccg cgaattgag aaggaggct gaagaaact gatcaaaac ataaaaatt gaagaaaaa ctgaacacc gtggatttg attgaacca caaaataat tttgaaagt ctaagaaat aaaagagat gtttcgaaa gttgaaagt acacttcaa cccgaacaa caagtacaa caacaaatt gatgaagtt gatagcaaa gtttcaaca caagttcaa cctgaacaa acaaaggaa ggtattgaa gagaaacaa caagtacag gtcgctgtg gatgaaagt gggcagcaa aacgttgaa cgctacact gatcaagtt caacattat aatcttgaa caaaaacat gtggatcaa ggacaattt gaacaacag tccactgaa caaactcag tttatccaa caacatgtg gatcaaggc gaatttgaa caacaacaa tacactgac caaggtcaa tttgaacag cagcactat gccaataat tctaatccg aaaactgaa caacagtat gaaaaaatt gaagaacca attcttcca acgcaacaa tatgatgat caagcccag tttgtacaa cagtttaat aaaactgag caacctgtt caagaacaa cattttgaa agtgagcag ccaatcccg tcacaacag tttgacgat caaggccaa tacgatgaa aatggacaa ctgattcaa gaacaacaa tatgatggt gaaccacaa ataccaacg caacaatat gacgatcaa ggtcaattt gtgcaacaa tatgatact agtgaacag cagttgtac gatcagagt ggacaacag tatgatgaa aacggacaa cctgtgcaa cagattact gaacaaggt caatttgtg caacagtac gacgaaagt ggacaacat attcaacag tacgatgaa caatacgga caacagtac gatcaacac ggccagtac ctacaacaa tatgacgaa accggacaa cctattcaa caatatgat gaaagtggg caacctctt caacaatac gacgaaagt ggacaacct attcaacag tatgacgga actgggcag tctattcaa cagtatgat gaacaacct gttcaacaa tacgacgaa agtggacaa cctattcaa cagtacgac gaaaatgga caacctatt caacagtac gacgaaact ggacaggct attcaacag tatggtgaa aatggacaa cctattcaa cagtatgac gaaactgga caatatggg caacattat gacgaaaac ggacaaccg actcaacag tttgatgat aatggacaa cctattcaa caatatgac gaaaatgcg caatactat ggcgatcaa agtaatgtg caatatgat gaaaacggt caaatgatt cagcattat gaccaaccg tatgatgga gtggttgaa catcagaat gaagtcgaa ggggacgat cagagtgtt cctgcaccg tcagaacca aagaaaagt aacattttg gaactgtta gatactgac actgaaagt atgaagcag gagtcgaaa gtttcacac gatagtgat tttgatttc agtaatgaa taagtttgg tagtttagc catagaaat gtaatgaat gacacactt gatttaggt ctgtgattc agttttttt aatacaaca ctaataaaa at
