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Gene information
General information
TC008920 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11908666..11911428
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008920
    iBeetle screen iB_04563

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008920 RA
      MSFGTEEYY KRFIELQEK LRKSEEERL KLEMKFNEM 
VQVTREEEQ MHYRKLRAQ YKRFLEEDR RRQDRNERI 
LRSLERIES RVAMLVAKT ERFKLLRQQ YQNFLDRIY 
RTKQAKIED VKPKEVEKP KTDDILQSY IQNLSSKKL 
REMLRKEED ETFDSGHPF EGDYRPNYA NSIADDIMS 
SIYSKPSKQ HPREFNNNN IWGAKMRAK SSTEDGSDI 
DEIPYNSGV SEAVKNFVR MGKRDSLVE QPTKIDHSL 
PRIEKEAEE TDQNIKIEE KLNTVDLIE PQNNFESLR 
NKRDVSKVE STLQPEQQV QQQIDEVDS KVSTQVQPE 
QTKEGIEEK QQVQVAVDE SGQQNVERY TDQVQHYNL 
EQKHVDQGQ FEQQSTEQT QFIQQHVDQ GEFEQQQYT 
DQGQFEQQH YANNSNPKT EQQYEKIEE PILPTQQYD 
DQAQFVQQF NKTEQPVQE QHFESEQPI PSQQFDDQG 
QYDENGQLI QEQQYDGEP QIPTQQYDD QGQFVQQYD 
TSEQQLYDQ SGQQYDENG QPVQQITEQ GQFVQQYDE 
SGQHIQQYD EQYGQQYDQ HGQYLQQYD ETGQPIQQY 
DESGQPLQQ YDESGQPIQ QYDGTGQSI QQYDEQPVQ 
QYDESGQPI QQYDENGQP IQQYDETGQ AIQQYGENG 
QPIQQYDET GQYGQHYDE NGQPTQQFD DNGQPIQQY 
DENAQYYGD QSNVQYDEN GQMIQHYDQ PYDGVVEHQ 
NEVEGDDQS VPAPSEPKK SNILELLDT DTESMKQES 
KVSHDSDFD FSNE
    CDS sequence
    • >TC008920 RA
      atgtcgttt gggaccgag gagtactac aagcgattc 
atcgaattg caggaaaag ctccgcaag agcgaggag 
gaacgcctc aaacttgaa atgaaattt aacgaaatg 
gttcaagtt acacgagag gaggaacaa atgcattat 
cggaaattg cgggctcaa tacaagcgg ttcttggag 
gaggacagg cggagacag gaccgaaat gagcgcatt 
ttacgcagt ttggagagg attgaaagt cgggttgcg 
atgctagtg gcgaagact gaacgattt aaactgttg 
agacagcag taccaaaat tttttggac cggatttat 
cgcaccaaa caagcaaaa attgaagat gtcaagccc 
aaagaagtc gagaaaccc aaaactgac gatattttg 
cagagttac atacaaaat ttgtcgtcg aagaaattg 
agggaaatg ttgaggaag gaggaagat gagactttc 
gattcgggt caccccttt gagggcgat tatagaccg 
aattatgcg aattccatc gccgatgat atcatgagt 
tcgatttat agcaaaccc agtaaacag caccctcga 
gagtttaat aataataat atttggggc gcgaaaatg 
cgggccaag agtagtact gaggacggt tccgacatt 
gacgaaatt ccgtacaac agtggtgtt agtgaagcc 
gtgaaaaat ttcgttcga atggggaaa agggacagt 
ttggttgaa caaccaacg aaaattgat cattccttg 
ccgcgaatt gagaaggag gctgaagaa actgatcaa 
aacataaaa attgaagaa aaactgaac accgtggat 
ttgattgaa ccacaaaat aattttgaa agtctaaga 
aataaaaga gatgtttcg aaagttgaa agtacactt 
caacccgaa caacaagta caacaacaa attgatgaa 
gttgatagc aaagtttca acacaagtt caacctgaa 
caaacaaag gaaggtatt gaagagaaa caacaagta 
caggtcgct gtggatgaa agtgggcag caaaacgtt 
gaacgctac actgatcaa gttcaacat tataatctt 
gaacaaaaa catgtggat caaggacaa tttgaacaa 
cagtccact gaacaaact cagtttatc caacaacat 
gtggatcaa ggcgaattt gaacaacaa caatacact 
gaccaaggt caatttgaa cagcagcac tatgccaat 
aattctaat ccgaaaact gaacaacag tatgaaaaa 
attgaagaa ccaattctt ccaacgcaa caatatgat 
gatcaagcc cagtttgta caacagttt aataaaact 
gagcaacct gttcaagaa caacatttt gaaagtgag 
cagccaatc ccgtcacaa cagtttgac gatcaaggc 
caatacgat gaaaatgga caactgatt caagaacaa 
caatatgat ggtgaacca caaatacca acgcaacaa 
tatgacgat caaggtcaa tttgtgcaa caatatgat 
actagtgaa cagcagttg tacgatcag agtggacaa 
cagtatgat gaaaacgga caacctgtg caacagatt 
actgaacaa ggtcaattt gtgcaacag tacgacgaa 
agtggacaa catattcaa cagtacgat gaacaatac 
ggacaacag tacgatcaa cacggccag tacctacaa 
caatatgac gaaaccgga caacctatt caacaatat 
gatgaaagt gggcaacct cttcaacaa tacgacgaa 
agtggacaa cctattcaa cagtatgac ggaactggg 
cagtctatt caacagtat gatgaacaa cctgttcaa 
caatacgac gaaagtgga caacctatt caacagtac 
gacgaaaat ggacaacct attcaacag tacgacgaa 
actggacag gctattcaa cagtatggt gaaaatgga 
caacctatt caacagtat gacgaaact ggacaatat 
gggcaacat tatgacgaa aacggacaa ccgactcaa 
cagtttgat gataatgga caacctatt caacaatat 
gacgaaaat gcgcaatac tatggcgat caaagtaat 
gtgcaatat gatgaaaac ggtcaaatg attcagcat 
tatgaccaa ccgtatgat ggagtggtt gaacatcag 
aatgaagtc gaaggggac gatcagagt gttcctgca 
ccgtcagaa ccaaagaaa agtaacatt ttggaactg 
ttagatact gacactgaa agtatgaag caggagtcg 
aaagtttca cacgatagt gattttgat ttcagtaat 
gaataa
    mRNA sequence
    • >TC008920 RA
      gtcgaactc cacccctag tggctttgt ttcgcccaa 
ttgtcttgt gattgcgat aacgagtga ttaattgaa 
atcggttca cacgcgaag ctttgatta aaacccgca 
attcgattg attcggtga aataaaatt gttgcattc 
gcctgtcgt gcgccaagt aattacgtc ccacatgca 
taatgtggt gcgttgctg gctagacca ctgcaaaac 
tagcttcaa attcgcgta attgtcaaa aaaaaaaac 
tgcgaaatg tcgtttggg accgaggag tactacaag 
cgattcatc gaattgcag gaaaagctc cgcaagagc 
gaggaggaa cgcctcaaa cttgaaatg aaatttaac 
gaaatggtt caagttaca cgagaggag gaacaaatg 
cattatcgg aaattgcgg gctcaatac aagcggttc 
ttggaggag gacaggcgg agacaggac cgaaatgag 
cgcatttta cgcagtttg gagaggatt gaaagtcgg 
gttgcgatg ctagtggcg aagactgaa cgatttaaa 
ctgttgaga cagcagtac caaaatttt ttggaccgg 
atttatcgc accaaacaa gcaaaaatt gaagatgtc 
aagcccaaa gaagtcgag aaacccaaa actgacgat 
attttgcag agttacata caaaatttg tcgtcgaag 
aaattgagg gaaatgttg aggaaggag gaagatgag 
actttcgat tcgggtcac ccctttgag ggcgattat 
agaccgaat tatgcgaat tccatcgcc gatgatatc 
atgagttcg atttatagc aaacccagt aaacagcac 
cctcgagag tttaataat aataatatt tggggcgcg 
aaaatgcgg gccaagagt agtactgag gacggttcc 
gacattgac gaaattccg tacaacagt ggtgttagt 
gaagccgtg aaaaatttc gttcgaatg gggaaaagg 
gacagtttg gttgaacaa ccaacgaaa attgatcat 
tccttgccg cgaattgag aaggaggct gaagaaact 
gatcaaaac ataaaaatt gaagaaaaa ctgaacacc 
gtggatttg attgaacca caaaataat tttgaaagt 
ctaagaaat aaaagagat gtttcgaaa gttgaaagt 
acacttcaa cccgaacaa caagtacaa caacaaatt 
gatgaagtt gatagcaaa gtttcaaca caagttcaa 
cctgaacaa acaaaggaa ggtattgaa gagaaacaa 
caagtacag gtcgctgtg gatgaaagt gggcagcaa 
aacgttgaa cgctacact gatcaagtt caacattat 
aatcttgaa caaaaacat gtggatcaa ggacaattt 
gaacaacag tccactgaa caaactcag tttatccaa 
caacatgtg gatcaaggc gaatttgaa caacaacaa 
tacactgac caaggtcaa tttgaacag cagcactat 
gccaataat tctaatccg aaaactgaa caacagtat 
gaaaaaatt gaagaacca attcttcca acgcaacaa 
tatgatgat caagcccag tttgtacaa cagtttaat 
aaaactgag caacctgtt caagaacaa cattttgaa 
agtgagcag ccaatcccg tcacaacag tttgacgat 
caaggccaa tacgatgaa aatggacaa ctgattcaa 
gaacaacaa tatgatggt gaaccacaa ataccaacg 
caacaatat gacgatcaa ggtcaattt gtgcaacaa 
tatgatact agtgaacag cagttgtac gatcagagt 
ggacaacag tatgatgaa aacggacaa cctgtgcaa 
cagattact gaacaaggt caatttgtg caacagtac 
gacgaaagt ggacaacat attcaacag tacgatgaa 
caatacgga caacagtac gatcaacac ggccagtac 
ctacaacaa tatgacgaa accggacaa cctattcaa 
caatatgat gaaagtggg caacctctt caacaatac 
gacgaaagt ggacaacct attcaacag tatgacgga 
actgggcag tctattcaa cagtatgat gaacaacct 
gttcaacaa tacgacgaa agtggacaa cctattcaa 
cagtacgac gaaaatgga caacctatt caacagtac 
gacgaaact ggacaggct attcaacag tatggtgaa 
aatggacaa cctattcaa cagtatgac gaaactgga 
caatatggg caacattat gacgaaaac ggacaaccg 
actcaacag tttgatgat aatggacaa cctattcaa 
caatatgac gaaaatgcg caatactat ggcgatcaa 
agtaatgtg caatatgat gaaaacggt caaatgatt 
cagcattat gaccaaccg tatgatgga gtggttgaa 
catcagaat gaagtcgaa ggggacgat cagagtgtt 
cctgcaccg tcagaacca aagaaaagt aacattttg 
gaactgtta gatactgac actgaaagt atgaagcag 
gagtcgaaa gtttcacac gatagtgat tttgatttc 
agtaatgaa taagtttgg tagtttagc catagaaat 
gtaatgaat gacacactt gatttaggt ctgtgattc 
agttttttt aatacaaca ctaataaaa at
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