Status
Please wait ...
Gene information
General information
TC008915 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11820753..11825836
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008915
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04562

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008915 RA
      MSFEYYSKW WAGTRNALE QLRAKDEIF VKATDKKLR 
      DRNLASHLI GGLYAKYSM MVQDLGTIL DQMAQPQKR 
      IIVRKLMDT ATVRLHELS EELRNVDIS EYHYIDGAL 
      IELKLVPQD VEILDPMLY YLRHYNMEE LWTKIKNGE 
      KIYTPPPPP PPPPQETEG EEGGEGETS KPKEIAEQT 
      PEEKAETEK KAKRRPKKF VPEVKELTE EELQEIKRK 
      EQITSAVIL IQNHERGRQ ARLYFDEKW KVNEYRRKI 
      REGFKPEPV DVETREKAS LHIQRYWRG HCARQLVKR 
      KELERRYLI KMIEPSWKS NEEFEKLER NLQKRRDIR 
      DQRIKEYIE ANLNERTRV FRVIGPRLM EDIGDEIRE 
      WFYQWYTQV KNFDKYPPE EKGGTVLVV RGETLTPKE 
      WIDEYNRKK REKVKNKGR DKEKLKAEK EKQKKLLAD 
      LKKKEKEKA KKEKLRKKK KKLKPWDFE LEYEPTVAK 
      EPYENAIEE YHRIFDERK DEDNPDEKH YMDIIHEEK 
      CYEMQLELR KVVDEMMRL ELEMLEDAL MKDRAKRKG 
      KKYKKKKKK KKKAKKRKK KGKKDPTGD RTVEDLFQE 
      LVDNGIIHP YKHHYLKEY KGDFSYNNW ERRNWGFDP 
      PATLGDVRQ AVKINCIAP LSTEKMIKP RSALLIGPR 
      QSGKHLLAN AVFTESKSV LFDLSPEVL AGKYPGKKG 
      MKMLLHLVN KMARILQPS VIYFDAAEK IFYKKVPRG 
      EKHLDPKRI GKKLQKGIV KTIKPEDRV MVFGISAQP 
      WLGKPGKMK KVFERIILI PRPDYGSIY QYWRELLMP 
      YHGIDRNID VSCLAKLTV NYPLPVLKQ VAEKVLTPR 
      RIIQLSYNP LRQSELYEE LITVDPITD KEYKKFVKF 
      YKKIPLMKE RGRLNKYLE KKREQESKA KEKQQQKAA 
      AKK
    CDS sequence
    • >TC008915 RA
      atgtctttc gaatattat tcgaaatgg tgggccggc 
      accagaaac gctctggag cagctgagg gccaaagac 
      gaaattttt gttaaagca actgataag aagctaaga 
      gaccggaac ttggccagt catttgatc ggagggttg 
      tatgcgaag tacagcatg atggtccag gatttgggc 
      acaattttg gaccaaatg gcccaaccc caaaaacgc 
      ataattgtg cgaaaacta atggacact gctacggtc 
      cgtcttcac gaactgagt gaagagcta agaaatgtt 
      gacatttcg gagtaccac tacatagac ggtgcctta 
      attgaactg aaactggtg ccccaagat gttgaaatt 
      ttagacccc atgttgtac tatctcaga cattataac 
      atggaagaa ctttggacc aagataaaa aatggcgaa 
      aaaatttac acaccccct cccccgcca ccaccccca 
      ccacaagaa acagagggt gaagaaggg ggcgaagga 
      gaaacatca aaacctaag gaaattgct gaacaaact 
      cctgaagag aaagccgaa acagaaaaa aaggctaag 
      agacgaccc aaaaaattt gtccctgaa gtaaaggaa 
      ctaacagag gaagagtta caagaaata aaacgtaaa 
      gaacaaatt acatccgcg gttatctta atacaaaat 
      catgaacgt ggcagacaa gctcgccta tactttgac 
      gaaaagtgg aaagtaaac gaatatcgg agaaaaata 
      agagaaggt tttaaacct gaaccagtt gatgtggaa 
      acgcgcgaa aaagcatcc cttcatatt cagagatac 
      tggcgagga cattgcgcc agacagctc gtgaaaaga 
      aaagaactg gaaagaaga tatttgatc aaaatgatc 
      gagccgagt tggaaatca aacgaagag ttcgaaaaa 
      ctagaaagg aatttacaa aaacggcgc gatatcagg 
      gaccaaaga attaaagaa tacattgaa gctaattta 
      aacgagaga acaagggtg ttcagagtt attggtcct 
      cgcttaatg gaagatata ggcgatgaa atcagagaa 
      tggttctat cagtggtac acccaagtc aaaaatttc 
      gacaagtac ccaccggaa gaaaaaggc ggaacggtt 
      ttagtagtc cgtggagaa acgctaacc ccgaaagaa 
      tggattgat gaatacaac aggaaaaaa agagaaaaa 
      gtcaaaaac aaagggcga gataaggaa aaattgaaa 
      gctgagaaa gagaagcag aagaaattg ttggctgat 
      ttgaagaaa aaagaaaaa gagaaagcc aaaaaagag 
      aaactgagg aagaagaag aaaaagctg aaaccttgg 
      gattttgaa ctcgaatat gaaccgaca gtcgccaaa 
      gaaccgtac gaaaatgcc atcgaggag taccaccga 
      atttttgac gaaagaaaa gatgaagat aatcccgat 
      gagaagcac tacatggat ataattcac gaagaaaag 
      tgctacgaa atgcaactg gagttgagg aaagttgtg 
      gacgagatg atgaggctt gagctcgaa atgttggaa 
      gacgcctta atgaaagac cgcgccaaa cgcaaaggc 
      aagaagtac aaaaagaaa aagaagaag aaaaagaaa 
      gccaaaaag aggaaaaag aagggcaaa aaggacccc 
      accggtgac aggaccgtg gaagatcta ttccaagaa 
      ttggtcgat aatgggata atacaccct tacaaacac 
      cactatctt aaagaatac aaaggggat tttagttac 
      aataactgg gagcgtaga aattggggt tttgatcct 
      ccggcaacc cttggtgac gtccggcaa gccgtcaaa 
      attaactgt attgcaccc ctaagtacc gaaaaaatg 
      attaaacca cggtcggct ctgttaatt gggccaaga 
      caaagtgga aaacacctt ctggcaaat gcggttttt 
      acggagagt aaaagtgtg ctttttgat ttgtcgccg 
      gaagttctc gccgggaaa tatccggga aaaaaaggg 
      atgaagatg ttgctgcat ttggtgaat aaaatggct 
      cggatttta caaccctca gttatttat tttgacgca 
      gctgagaaa attttctac aagaaagtg ccaagagga 
      gagaagcac ttggatccg aaacggatc ggtaaaaag 
      cttcagaaa ggtatcgtc aagaccatt aaacccgaa 
      gatcgggtc atggttttc gggataagt gcccaacct 
      tggttagga aagcccgga aaaatgaag aaagttttc 
      gaaagaatt attttgatt cctcgtcct gattacggg 
      agtatttat cagtattgg agggagttg ttgatgcct 
      tatcacggc atcgaccga aatattgac gtttcttgt 
      ttggccaag ctcacggtg aattacccc ttgcccgtg 
      ctgaagcaa gtggctgaa aaagtgttg actcctcgc 
      aggataatc cagcttagt tacaatcct ttgaggcag 
      tccgagctg tacgaggag ctaataacc gtggatcca 
      attacggat aaagagtac aagaaattc gtaaagttt 
      tataagaaa attcctttg atgaaggaa cggggaagg 
      ctgaacaag tacttggag aagaagcga gaacaagag 
      tccaaagct aaggaaaag cagcagcag aaagcggcg 
      gctaagaaa tga
    mRNA sequence
    • >TC008915 RA
      ttcagtgat tttttatta ttttttggc aatctattt 
      gacggcaat atttaggca acgtaatcg ctttttcgg 
      ccccaagga aaatgtaaa atttaagta aaatgtctt 
      tcgaatatt attcgaaat ggtgggccg gcaccagaa 
      acgctctgg agcagctga gggccaaag acgaaattt 
      ttgttaaag caactgata agaagctaa gagaccgga 
      acttggcca gtcatttga tcggagggt tgtatgcga 
      agtacagca tgatggtcc aggatttgg gcacaattt 
      tggaccaaa tggcccaac cccaaaaac gcataattg 
      tgcgaaaac taatggaca ctgctacgg tccgtcttc 
      acgaactga gtgaagagc taagaaatg ttgacattt 
      cggagtacc actacatag acggtgcct taattgaac 
      tgaaactgg tgccccaag atgttgaaa ttttagacc 
      ccatgttgt actatctca gacattata acatggaag 
      aactttgga ccaagataa aaaatggcg aaaaaattt 
      acacacccc ctcccccgc caccacccc caccacaag 
      aaacagagg gtgaagaag ggggcgaag gagaaacat 
      caaaaccta aggaaattg ctgaacaaa ctcctgaag 
      agaaagccg aaacagaaa aaaaggcta agagacgac 
      ccaaaaaat ttgtccctg aagtaaagg aactaacag 
      aggaagagt tacaagaaa taaaacgta aagaacaaa 
      ttacatccg cggttatct taatacaaa atcatgaac 
      gtggcagac aagctcgcc tatactttg acgaaaagt 
      ggaaagtaa acgaatatc ggagaaaaa taagagaag 
      gttttaaac ctgaaccag ttgatgtgg aaacgcgcg 
      aaaaagcat cccttcata ttcagagat actggcgag 
      gacattgcg ccagacagc tcgtgaaaa gaaaagaac 
      tggaaagaa gatatttga tcaaaatga tcgagccga 
      gttggaaat caaacgaag agttcgaaa aactagaaa 
      ggaatttac aaaaacggc gcgatatca gggaccaaa 
      gaattaaag aatacattg aagctaatt taaacgaga 
      gaacaaggg tgttcagag ttattggtc ctcgcttaa 
      tggaagata taggcgatg aaatcagag aatggttct 
      atcagtggt acacccaag tcaaaaatt tcgacaagt 
      acccaccgg aagaaaaag gcggaacgg ttttagtag 
      tccgtggag aaacgctaa ccccgaaag aatggattg 
      atgaataca acaggaaaa aaagagaaa aagtcaaaa 
      acaaagggc gagataagg aaaaattga aagctgaga 
      aagagaagc agaagaaat tgttggctg atttgaaga 
      aaaaagaaa aagagaaag ccaaaaaag agaaactga 
      ggaagaaga agaaaaagc tgaaacctt gggattttg 
      aactcgaat atgaaccga cagtcgcca aagaaccgt 
      acgaaaatg ccatcgagg agtaccacc gaatttttg 
      acgaaagaa aagatgaag ataatcccg atgagaagc 
      actacatgg atataattc acgaagaaa agtgctacg 
      aaatgcaac tggagttga ggaaagttg tggacgaga 
      tgatgaggc ttgagctcg aaatgttgg aagacgcct 
      taatgaaag accgcgcca aacgcaaag gcaagaagt 
      acaaaaaga aaaagaaga agaaaaaga aagccaaaa 
      agaggaaaa agaagggca aaaaggacc ccaccggtg 
      acaggaccg tggaagatc tattccaag aattggtcg 
      ataatggga taatacacc cttacaaac accactatc 
      ttaaagaat acaaagggg attttagtt acaataact 
      gggagcgta gaaattggg gttttgatc ctccggcaa 
      cccttggtg acgtccggc aagccgtca aaattaact 
      gtattgcac ccctaagta ccgaaaaaa tgattaaac 
      cacggtcgg ctctgttaa ttgggccaa gacaaagtg 
      gaaaacacc ttctggcaa atgcggttt ttacggaga 
      gtaaaagtg tgctttttg atttgtcgc cggaagttc 
      tcgccggga aatatccgg gaaaaaaag ggatgaaga 
      tgttgctgc atttggtga ataaaatgg ctcggattt 
      tacaaccct cagttattt attttgacg cagctgaga 
      aaattttct acaagaaag tgccaagag gagagaagc 
      acttggatc cgaaacgga tcggtaaaa agcttcaga 
      aaggtatcg tcaagacca ttaaacccg aagatcggg 
      tcatggttt tcgggataa gtgcccaac cttggttag 
      gaaagcccg gaaaaatga agaaagttt tcgaaagaa 
      ttattttga ttcctcgtc ctgattacg ggagtattt 
      atcagtatt ggagggagt tgttgatgc cttatcacg 
      gcatcgacc gaaatattg acgtttctt gtttggcca 
      agctcacgg tgaattacc ccttgcccg tgctgaagc 
      aagtggctg aaaaagtgt tgactcctc gcaggataa 
      tccagctta gttacaatc ctttgaggc agtccgagc 
      tgtacgagg agctaataa ccgtggatc caattacgg 
      ataaagagt acaagaaat tcgtaaagt tttataaga 
      aaattcctt tgatgaagg aacggggaa ggctgaaca 
      agtacttgg agaagaagc gagaacaag agtccaaag 
      ctaaggaaa agcagcagc agaaagcgg cggctaaga 
      aatgaccgt gtttgcgtc ttattgtat ctcgttttg 
      ttttgtatt atagtgtta ttgatcgag tgagggctt 
      tgtaatgag cactaagaa attccgaac aaggga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY