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Gene information
General information
TC008909 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11654462..11668994
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008909
    iBeetle screen iB_04561

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008909 RA
      MDNFQELFS GGETWPTDS RDIRPDNIS STDSATPPP 
      QIDDKDDKK SANSIRAQI EIIPCKVCG DKSSGVHYG 
      VITCEGCKG FFRRSQSSV VNYQCPRNK NCVVDRVNR 
      NRCQYCRLQ KCLRLGMSR DAVKFGRMS KKQREKVED 
      EVRFHRAQL RAQSDSAPD SSVFEQQTP SSSDQLHHS 
      YNGGYTYNS DVSAYTPAY YTNTTQVQA NSMGYDISA 
      DYVDSTTAY DPRPGLDTL NDTGSLMAN VVTTDPSQI 
      SELLSKTIS DAHTRTCLY SLEHIHEMF RKPHDLSRL 
      LYYKNMAHE ELWLECAQK LTTIIQQII EFAKMVPGF 
      MKLSQDDQI VLLKAGSFE LAIIRMSRY LDLSQNCVL 
      YGDTMLPQD AFFTSDTAE MKLVTCVFE VSKSIAELK 
      LTETELALY SACVLLSPD RPGLKGIGE ISRLSQAVV 
      RALRLELDR NHSMPIKGD VTVCDALLT KIPTLREIS 
      MLHMDALGK LKRSAPHLE FPALHKELF SVDS
    CDS sequence
    • >TC008909 RA
      atggacaat tttcaagag ttgttcagt ggtggggaa 
      acttggccg actgactca agggatatt agaccggat 
      aatatttcc tcgacggac tcagcaacc ccaccccct 
      caaatcgac gacaaggat gacaagaaa agcgccaat 
      tccatcaga gcacaaatc gagataatc ccgtgcaaa 
      gtatgtggg gacaagagc agcggtgtt cactatggt 
      gtgattaca tgcgaggga tgtaagggg tttttccga 
      agatcacag agttcagtt gtaaactat cagtgtcct 
      agaaataaa aactgtgtt gtcgacagg gtaaatagg 
      aatcgatgt caatactgc cgactgcag aaatgtctt 
      cggctggga atgagccga gatgctgtc aaattcggc 
      cgaatgtcg aagaagcag cgagagaaa gtagaagac 
      gaagtcaga tttcatagg gcacagtta agggcgcag 
      agcgattct gctcctgat agttcagtg ttcgaacaa 
      caaacaccc tcaagtagt gatcagtta catcatagt 
      tacaatggc gggtatacg tacaacagc gatgtcagc 
      gcgtacact ccagcctat tacacgaat acaacccaa 
      gtccaagcg aattcgatg ggctatgac atctccgca 
      gattacgtg gacagtaca acagcgtat gatccgagg 
      ccgggcctg gacacactg aacgacacg ggaagctta 
      atggccaac gttgtaaca acagatcca agtcagata 
      tcggagtta ttatcgaag acaatatca gacgcacac 
      acacgtact tgtttatat tccctcgaa catattcac 
      gagatgttc aggaaaccc catgatctc tcccgactt 
      ttgtactat aagaacatg gcccatgaa gagttgtgg 
      ctcgaatgc gcccaaaaa ctcacaaca atcatccag 
      caaatcatc gaatttgcg aaaatggta cctggattc 
      atgaaattg tcccaagac gatcagatc gtattattg 
      aaagcaggt agttttgaa ctggcaatt attagaatg 
      tcacgttat ttggatctg tcccaaaac tgtgttctc 
      tacggcgat acgatgctc ccccaagac gccttcttc 
      acgtcggat acggcggaa atgaagctg gtcacgtgc 
      gtcttcgaa gtgtccaaa agcatagca gaactgaaa 
      cttaccgaa acagagttg gctctgtat tcggcctgc 
      gtcttgctc tctccagat cggccgggg ctgaaaggt 
      atcggcgaa atcagtcga cttagccaa gccgtcgtg 
      agagcctta agacttgag ctagacaga aaccacagc 
      atgcctata aaaggggat gttacagtt tgtgatgcc 
      ttgcttaca aaaatacct acgttaagg gagataagt 
      atgcttcac atggatgca ttaggaaaa ctcaaaaga 
      tctgcacca cacctggag tttcccgcc cttcacaag 
      gagttgttt agtgtggac agttga
    mRNA sequence
    • >TC008909 RA
      ggatacggc tacgtcacg gagccggcg ctaagacct 
      acgttttta tttacatcg aatgcgtta gataaggac 
      gaaatgctg gcagtcggt ctgcgctcc aaggcagac 
      tttcttaaa aaatgtcca aaatatgtg ccggtttta 
      cgcaagata tcgacggca aggtccgcc acggcaagt 
      gaactcgcg cagtgtccc agggattta attaaaatc 
      ccacttgag cacagtggc tgttttttc tcagtaaat 
      cattttttg atcctcggt gcagtgagt gacagttac 
      ctgcaataa cggctcgag tgacgtttg tgcttttgt 
      gtggatttt ccgggaaaa tttcggatt cgaaggcag 
      tgttttgca atattgcac gctccgaga tagcgtata 
      aaatagcag aagtgaaac tctccgtcc tgagccgag 
      taattatga ttttggggt tgacgattg gcaccactg 
      tccgcgggg cgacgtctg atccaggga aaaaattcc 
      gcgtgcact taaatttta atgattatt gataataat 
      gttgttgtt ttatcgtaa tcggtcgtt cacattcgc 
      gtaaaaata gacgagtgc ttatgcgca accgacttt 
      gtcttgaag gcgattttt ccgttggtg gtcattttt 
      cggaattct cccgtatta acgatcgca tctctcgct 
      caaccatat tcggcggtg aacaaccta gagttcaat 
      ggcttgccg cgcctccaa acacaaacc gtcgtactt 
      tatgccggt ttgagccaa acaaaccgt attaatggg 
      ccgcccttt cgaatttcc ctcattccg gcactgcag 
      catccccga gctggcagc cctggcgtg atttttcga 
      aatttccga cgccgtgcg cctcgaggt caacgaccc 
      cgcgcgcag ccagatttt actttgttg aatttacca 
      atgacccct tgtacggcg ccttatcac actcctact 
      gagtaaaga ctcatgtat gagccatcc caaattgat 
      tagcaccga cacaggttg ggcgaagag aatggagcc 
      gaaagttgg tgcttcggc ggctcattc tgatgagta 
      aaaacgtga tcgatctcc ctcgttcgt gtttttctc 
      agtttcagg tgtctgacg atggaaatc gacgtcgtc 
      aatatcgac cttaaacta gtccatcga tcttgcgtg 
      aaataaaag tggttcaaa gtttgtgcg agtggcgaa 
      ttgtggctg cggccatgc gacgcactt ctggatttg 
      ccattaaaa tacgaggtg ccagtgatc gaacgttag 
      tgtttttgt ttttgtaca atggacaat tttcaagag 
      ttgttcagt ggtggggaa acttggccg actgactca 
      agggatatt agaccggat aatatttcc tcgacggac 
      tcagcaacc ccaccccct caaatcgac gacaaggat 
      gacaagaaa agcgccaat tccatcaga gcacaaatc 
      gagataatc ccgtgcaaa gtatgtggg gacaagagc 
      agcggtgtt cactatggt gtgattaca tgcgaggga 
      tgtaagggg tttttccga agatcacag agttcagtt 
      gtaaactat cagtgtcct agaaataaa aactgtgtt 
      gtcgacagg gtaaatagg aatcgatgt caatactgc 
      cgactgcag aaatgtctt cggctggga atgagccga 
      gatgctgtc aaattcggc cgaatgtcg aagaagcag 
      cgagagaaa gtagaagac gaagtcaga tttcatagg 
      gcacagtta agggcgcag agcgattct gctcctgat 
      agttcagtg ttcgaacaa caaacaccc tcaagtagt 
      gatcagtta catcatagt tacaatggc gggtatacg 
      tacaacagc gatgtcagc gcgtacact ccagcctat 
      tacacgaat acaacccaa gtccaagcg aattcgatg 
      ggctatgac atctccgca gattacgtg gacagtaca 
      acagcgtat gatccgagg ccgggcctg gacacactg 
      aacgacacg ggaagctta atggccaac gttgtaaca 
      acagatcca agtcagata tcggagtta ttatcgaag 
      acaatatca gacgcacac acacgtact tgtttatat 
      tccctcgaa catattcac gagatgttc aggaaaccc 
      catgatctc tcccgactt ttgtactat aagaacatg 
      gcccatgaa gagttgtgg ctcgaatgc gcccaaaaa 
      ctcacaaca atcatccag caaatcatc gaatttgcg 
      aaaatggta cctggattc atgaaattg tcccaagac 
      gatcagatc gtattattg aaagcaggt agttttgaa 
      ctggcaatt attagaatg tcacgttat ttggatctg 
      tcccaaaac tgtgttctc tacggcgat acgatgctc 
      ccccaagac gccttcttc acgtcggat acggcggaa 
      atgaagctg gtcacgtgc gtcttcgaa gtgtccaaa 
      agcatagca gaactgaaa cttaccgaa acagagttg 
      gctctgtat tcggcctgc gtcttgctc tctccagat 
      cggccgggg ctgaaaggt atcggcgaa atcagtcga 
      cttagccaa gccgtcgtg agagcctta agacttgag 
      ctagacaga aaccacagc atgcctata aaaggggat 
      gttacagtt tgtgatgcc ttgcttaca aaaatacct 
      acgttaagg gagataagt atgcttcac atggatgca 
      ttaggaaaa ctcaaaaga tctgcacca cacctggag 
      tttcccgcc cttcacaag gagttgttt agtgtggac 
      agttgaaaa acttcagca gaatcttct gatccgtag 
      caacatagc tactgtaag tatttctca attactgga 
      aagaaacca caaaacgtt acgtccaaa taacaaaat 
      tttacggat ttgtttgtt gcagattcc ttcatactg 
      aatgtatcg gccttaaat acataaata taaagtata 
      atttcgtta taatgagat aaaatttat tgttgtgac 
      atagaaata aagaaccgc agatctcta agtattatt 
      taaaaaact gcaaaagct ctatgaagc cgggccctg 
      atttcaaag tacaactta ttgtttgtt gtttgttat 
      tatatctac gcagtgtgt tattatatg gttctgaat 
      cctaggtgc tgaataaaa tcgattggt atatttttg 
      cggtttttt aaatgaaat atttacgta tcgtaatct 
      gccacttaa cgtaacacg ctaattaca cgtaagatt 
      aaagttata cactattga tatataata tatgaccaa 
      caagttata agtcagtga aactcttgt ttagtttta 
      cgtatttaa agaaaaaat catacttag actgcgttt 
      atattataa taatacgta agtatactg aaatcactt 
      tagttggta gtgttgaaa tagataaca tacaataca 
      ttataaata tagacaaga ctgtatgct tacactgta 
      gagttttta actggtttt taacttgtt gttttttag 
      ttaagtttt acttggtga cacatggtt ttataaaaa 
      tatatataa aagagatgt atatgtata aagtatatg 
      aaatagtat atagagtat ttatgaaat agaactata 
      tctgattct tgttttacg gattttgtt ttattatgg 
      attcgaaca taatttttt ttccataat aaaatgaaa 
      attttgcga tcgtgataa cagactttt ttcagcttt 
      tacaattta tagatttgt tagatttgc aaggaaatt 
      acacgaaca cggagaaca aacttggat cgcagcagc 
      tacttgcct ctcttcatt tttctcggt attaatgtc 
      aaaagttaa gtatgttta tgtaatttc ccgtcgaaa 
      atgccagaa tgaatagtt ctaattttt aagttagtt 
      ttaacgtgc ttgtacatg ttaaccatt gttaaatct 
      cattttaac ttagtactt tcaacccag tgttataaa 
      gctatttat gtaattaga atctttgta ggtatgaaa 
      caaacctaa catcacatt ttttttaaa caaaaactc 
      aaaggtctt gtattatag aaaattttt gtattttaa 
      taataattg tagtttctt ccgttttga caatggtta 
      acatccatg agaggctat tggttattt ttgtattta 
      acgaaccat gtaatttta gaaataact attttttgg 
      agattacta taaagacat ggctgtgtt acatacatg 
      ataacagtt ttacgaagt gtaatttgc cttttttgt 
      gttaacttt ttgttaaat aaagaaatt acaatcttc 
      cacaatatt ttattttaa atataatat ttttgcaaa 
      catatcact aattccgcg ctatgttgt atttctcgt 
      tgacaattt atttaacgt aaagttaac aaatttgcg 
      agttgcaag tgcgtaaaa atagccact tttgtaaat 
      gagggttgg cagtgggta atggtatag ctattgtta 
      aatgattat tatgaacta aaatgttta aaaaacatg 
      taccttggg ctacaaact gtacattat cttaattta 
      ttagaatcc tttatactg ctggtgaaa tttttttag 
      tgtagatgt tgctaacat tttgtttat attatatat 
      attatatat attttattt ctgttggtc tatgacaat 
      tattacagt ttgttaata tttttgtta gtattttat 
      ttttcctcc taataatct ctttctgta gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY