| General information | |||
| TC008909 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 11654462..11668994 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008909 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04561 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC008909 RA
MDNFQELFS GGETWPTDS RDIRPDNIS STDSATPPP QIDDKDDKK SANSIRAQI EIIPCKVCG DKSSGVHYG VITCEGCKG FFRRSQSSV VNYQCPRNK NCVVDRVNR NRCQYCRLQ KCLRLGMSR DAVKFGRMS KKQREKVED EVRFHRAQL RAQSDSAPD SSVFEQQTP SSSDQLHHS YNGGYTYNS DVSAYTPAY YTNTTQVQA NSMGYDISA DYVDSTTAY DPRPGLDTL NDTGSLMAN VVTTDPSQI SELLSKTIS DAHTRTCLY SLEHIHEMF RKPHDLSRL LYYKNMAHE ELWLECAQK LTTIIQQII EFAKMVPGF MKLSQDDQI VLLKAGSFE LAIIRMSRY LDLSQNCVL YGDTMLPQD AFFTSDTAE MKLVTCVFE VSKSIAELK LTETELALY SACVLLSPD RPGLKGIGE ISRLSQAVV RALRLELDR NHSMPIKGD VTVCDALLT KIPTLREIS MLHMDALGK LKRSAPHLE FPALHKELF SVDS
- >TC008909 RA
atggacaat tttcaagag ttgttcagt ggtggggaa acttggccg actgactca agggatatt agaccggat aatatttcc tcgacggac tcagcaacc ccaccccct caaatcgac gacaaggat gacaagaaa agcgccaat tccatcaga gcacaaatc gagataatc ccgtgcaaa gtatgtggg gacaagagc agcggtgtt cactatggt gtgattaca tgcgaggga tgtaagggg tttttccga agatcacag agttcagtt gtaaactat cagtgtcct agaaataaa aactgtgtt gtcgacagg gtaaatagg aatcgatgt caatactgc cgactgcag aaatgtctt cggctggga atgagccga gatgctgtc aaattcggc cgaatgtcg aagaagcag cgagagaaa gtagaagac gaagtcaga tttcatagg gcacagtta agggcgcag agcgattct gctcctgat agttcagtg ttcgaacaa caaacaccc tcaagtagt gatcagtta catcatagt tacaatggc gggtatacg tacaacagc gatgtcagc gcgtacact ccagcctat tacacgaat acaacccaa gtccaagcg aattcgatg ggctatgac atctccgca gattacgtg gacagtaca acagcgtat gatccgagg ccgggcctg gacacactg aacgacacg ggaagctta atggccaac gttgtaaca acagatcca agtcagata tcggagtta ttatcgaag acaatatca gacgcacac acacgtact tgtttatat tccctcgaa catattcac gagatgttc aggaaaccc catgatctc tcccgactt ttgtactat aagaacatg gcccatgaa gagttgtgg ctcgaatgc gcccaaaaa ctcacaaca atcatccag caaatcatc gaatttgcg aaaatggta cctggattc atgaaattg tcccaagac gatcagatc gtattattg aaagcaggt agttttgaa ctggcaatt attagaatg tcacgttat ttggatctg tcccaaaac tgtgttctc tacggcgat acgatgctc ccccaagac gccttcttc acgtcggat acggcggaa atgaagctg gtcacgtgc gtcttcgaa gtgtccaaa agcatagca gaactgaaa cttaccgaa acagagttg gctctgtat tcggcctgc gtcttgctc tctccagat cggccgggg ctgaaaggt atcggcgaa atcagtcga cttagccaa gccgtcgtg agagcctta agacttgag ctagacaga aaccacagc atgcctata aaaggggat gttacagtt tgtgatgcc ttgcttaca aaaatacct acgttaagg gagataagt atgcttcac atggatgca ttaggaaaa ctcaaaaga tctgcacca cacctggag tttcccgcc cttcacaag gagttgttt agtgtggac agttga
- >TC008909 RA
ggatacggc tacgtcacg gagccggcg ctaagacct acgttttta tttacatcg aatgcgtta gataaggac gaaatgctg gcagtcggt ctgcgctcc aaggcagac tttcttaaa aaatgtcca aaatatgtg ccggtttta cgcaagata tcgacggca aggtccgcc acggcaagt gaactcgcg cagtgtccc agggattta attaaaatc ccacttgag cacagtggc tgttttttc tcagtaaat cattttttg atcctcggt gcagtgagt gacagttac ctgcaataa cggctcgag tgacgtttg tgcttttgt gtggatttt ccgggaaaa tttcggatt cgaaggcag tgttttgca atattgcac gctccgaga tagcgtata aaatagcag aagtgaaac tctccgtcc tgagccgag taattatga ttttggggt tgacgattg gcaccactg tccgcgggg cgacgtctg atccaggga aaaaattcc gcgtgcact taaatttta atgattatt gataataat gttgttgtt ttatcgtaa tcggtcgtt cacattcgc gtaaaaata gacgagtgc ttatgcgca accgacttt gtcttgaag gcgattttt ccgttggtg gtcattttt cggaattct cccgtatta acgatcgca tctctcgct caaccatat tcggcggtg aacaaccta gagttcaat ggcttgccg cgcctccaa acacaaacc gtcgtactt tatgccggt ttgagccaa acaaaccgt attaatggg ccgcccttt cgaatttcc ctcattccg gcactgcag catccccga gctggcagc cctggcgtg atttttcga aatttccga cgccgtgcg cctcgaggt caacgaccc cgcgcgcag ccagatttt actttgttg aatttacca atgacccct tgtacggcg ccttatcac actcctact gagtaaaga ctcatgtat gagccatcc caaattgat tagcaccga cacaggttg ggcgaagag aatggagcc gaaagttgg tgcttcggc ggctcattc tgatgagta aaaacgtga tcgatctcc ctcgttcgt gtttttctc agtttcagg tgtctgacg atggaaatc gacgtcgtc aatatcgac cttaaacta gtccatcga tcttgcgtg aaataaaag tggttcaaa gtttgtgcg agtggcgaa ttgtggctg cggccatgc gacgcactt ctggatttg ccattaaaa tacgaggtg ccagtgatc gaacgttag tgtttttgt ttttgtaca atggacaat tttcaagag ttgttcagt ggtggggaa acttggccg actgactca agggatatt agaccggat aatatttcc tcgacggac tcagcaacc ccaccccct caaatcgac gacaaggat gacaagaaa agcgccaat tccatcaga gcacaaatc gagataatc ccgtgcaaa gtatgtggg gacaagagc agcggtgtt cactatggt gtgattaca tgcgaggga tgtaagggg tttttccga agatcacag agttcagtt gtaaactat cagtgtcct agaaataaa aactgtgtt gtcgacagg gtaaatagg aatcgatgt caatactgc cgactgcag aaatgtctt cggctggga atgagccga gatgctgtc aaattcggc cgaatgtcg aagaagcag cgagagaaa gtagaagac gaagtcaga tttcatagg gcacagtta agggcgcag agcgattct gctcctgat agttcagtg ttcgaacaa caaacaccc tcaagtagt gatcagtta catcatagt tacaatggc gggtatacg tacaacagc gatgtcagc gcgtacact ccagcctat tacacgaat acaacccaa gtccaagcg aattcgatg ggctatgac atctccgca gattacgtg gacagtaca acagcgtat gatccgagg ccgggcctg gacacactg aacgacacg ggaagctta atggccaac gttgtaaca acagatcca agtcagata tcggagtta ttatcgaag acaatatca gacgcacac acacgtact tgtttatat tccctcgaa catattcac gagatgttc aggaaaccc catgatctc tcccgactt ttgtactat aagaacatg gcccatgaa gagttgtgg ctcgaatgc gcccaaaaa ctcacaaca atcatccag caaatcatc gaatttgcg aaaatggta cctggattc atgaaattg tcccaagac gatcagatc gtattattg aaagcaggt agttttgaa ctggcaatt attagaatg tcacgttat ttggatctg tcccaaaac tgtgttctc tacggcgat acgatgctc ccccaagac gccttcttc acgtcggat acggcggaa atgaagctg gtcacgtgc gtcttcgaa gtgtccaaa agcatagca gaactgaaa cttaccgaa acagagttg gctctgtat tcggcctgc gtcttgctc tctccagat cggccgggg ctgaaaggt atcggcgaa atcagtcga cttagccaa gccgtcgtg agagcctta agacttgag ctagacaga aaccacagc atgcctata aaaggggat gttacagtt tgtgatgcc ttgcttaca aaaatacct acgttaagg gagataagt atgcttcac atggatgca ttaggaaaa ctcaaaaga tctgcacca cacctggag tttcccgcc cttcacaag gagttgttt agtgtggac agttgaaaa acttcagca gaatcttct gatccgtag caacatagc tactgtaag tatttctca attactgga aagaaacca caaaacgtt acgtccaaa taacaaaat tttacggat ttgtttgtt gcagattcc ttcatactg aatgtatcg gccttaaat acataaata taaagtata atttcgtta taatgagat aaaatttat tgttgtgac atagaaata aagaaccgc agatctcta agtattatt taaaaaact gcaaaagct ctatgaagc cgggccctg atttcaaag tacaactta ttgtttgtt gtttgttat tatatctac gcagtgtgt tattatatg gttctgaat cctaggtgc tgaataaaa tcgattggt atatttttg cggtttttt aaatgaaat atttacgta tcgtaatct gccacttaa cgtaacacg ctaattaca cgtaagatt aaagttata cactattga tatataata tatgaccaa caagttata agtcagtga aactcttgt ttagtttta cgtatttaa agaaaaaat catacttag actgcgttt atattataa taatacgta agtatactg aaatcactt tagttggta gtgttgaaa tagataaca tacaataca ttataaata tagacaaga ctgtatgct tacactgta gagttttta actggtttt taacttgtt gttttttag ttaagtttt acttggtga cacatggtt ttataaaaa tatatataa aagagatgt atatgtata aagtatatg aaatagtat atagagtat ttatgaaat agaactata tctgattct tgttttacg gattttgtt ttattatgg attcgaaca taatttttt ttccataat aaaatgaaa attttgcga tcgtgataa cagactttt ttcagcttt tacaattta tagatttgt tagatttgc aaggaaatt acacgaaca cggagaaca aacttggat cgcagcagc tacttgcct ctcttcatt tttctcggt attaatgtc aaaagttaa gtatgttta tgtaatttc ccgtcgaaa atgccagaa tgaatagtt ctaattttt aagttagtt ttaacgtgc ttgtacatg ttaaccatt gttaaatct cattttaac ttagtactt tcaacccag tgttataaa gctatttat gtaattaga atctttgta ggtatgaaa caaacctaa catcacatt ttttttaaa caaaaactc aaaggtctt gtattatag aaaattttt gtattttaa taataattg tagtttctt ccgttttga caatggtta acatccatg agaggctat tggttattt ttgtattta acgaaccat gtaatttta gaaataact attttttgg agattacta taaagacat ggctgtgtt acatacatg ataacagtt ttacgaagt gtaatttgc cttttttgt gttaacttt ttgttaaat aaagaaatt acaatcttc cacaatatt ttattttaa atataatat ttttgcaaa catatcact aattccgcg ctatgttgt atttctcgt tgacaattt atttaacgt aaagttaac aaatttgcg agttgcaag tgcgtaaaa atagccact tttgtaaat gagggttgg cagtgggta atggtatag ctattgtta aatgattat tatgaacta aaatgttta aaaaacatg taccttggg ctacaaact gtacattat cttaattta ttagaatcc tttatactg ctggtgaaa tttttttag tgtagatgt tgctaacat tttgtttat attatatat attatatat attttattt ctgttggtc tatgacaat tattacagt ttgttaata tttttgtta gtattttat ttttcctcc taataatct ctttctgta gtt
