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Gene information
General information
TC008909 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11654462..11668994
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008909
    iBeetle screen iB_04561

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008909 RA
      MDNFQELFS GGETWPTDS RDIRPDNIS STDSATPPP 
QIDDKDDKK SANSIRAQI EIIPCKVCG DKSSGVHYG 
VITCEGCKG FFRRSQSSV VNYQCPRNK NCVVDRVNR 
NRCQYCRLQ KCLRLGMSR DAVKFGRMS KKQREKVED 
EVRFHRAQL RAQSDSAPD SSVFEQQTP SSSDQLHHS 
YNGGYTYNS DVSAYTPAY YTNTTQVQA NSMGYDISA 
DYVDSTTAY DPRPGLDTL NDTGSLMAN VVTTDPSQI 
SELLSKTIS DAHTRTCLY SLEHIHEMF RKPHDLSRL 
LYYKNMAHE ELWLECAQK LTTIIQQII EFAKMVPGF 
MKLSQDDQI VLLKAGSFE LAIIRMSRY LDLSQNCVL 
YGDTMLPQD AFFTSDTAE MKLVTCVFE VSKSIAELK 
LTETELALY SACVLLSPD RPGLKGIGE ISRLSQAVV 
RALRLELDR NHSMPIKGD VTVCDALLT KIPTLREIS 
MLHMDALGK LKRSAPHLE FPALHKELF SVDS
    CDS sequence
    • >TC008909 RA
      atggacaat tttcaagag ttgttcagt ggtggggaa 
acttggccg actgactca agggatatt agaccggat 
aatatttcc tcgacggac tcagcaacc ccaccccct 
caaatcgac gacaaggat gacaagaaa agcgccaat 
tccatcaga gcacaaatc gagataatc ccgtgcaaa 
gtatgtggg gacaagagc agcggtgtt cactatggt 
gtgattaca tgcgaggga tgtaagggg tttttccga 
agatcacag agttcagtt gtaaactat cagtgtcct 
agaaataaa aactgtgtt gtcgacagg gtaaatagg 
aatcgatgt caatactgc cgactgcag aaatgtctt 
cggctggga atgagccga gatgctgtc aaattcggc 
cgaatgtcg aagaagcag cgagagaaa gtagaagac 
gaagtcaga tttcatagg gcacagtta agggcgcag 
agcgattct gctcctgat agttcagtg ttcgaacaa 
caaacaccc tcaagtagt gatcagtta catcatagt 
tacaatggc gggtatacg tacaacagc gatgtcagc 
gcgtacact ccagcctat tacacgaat acaacccaa 
gtccaagcg aattcgatg ggctatgac atctccgca 
gattacgtg gacagtaca acagcgtat gatccgagg 
ccgggcctg gacacactg aacgacacg ggaagctta 
atggccaac gttgtaaca acagatcca agtcagata 
tcggagtta ttatcgaag acaatatca gacgcacac 
acacgtact tgtttatat tccctcgaa catattcac 
gagatgttc aggaaaccc catgatctc tcccgactt 
ttgtactat aagaacatg gcccatgaa gagttgtgg 
ctcgaatgc gcccaaaaa ctcacaaca atcatccag 
caaatcatc gaatttgcg aaaatggta cctggattc 
atgaaattg tcccaagac gatcagatc gtattattg 
aaagcaggt agttttgaa ctggcaatt attagaatg 
tcacgttat ttggatctg tcccaaaac tgtgttctc 
tacggcgat acgatgctc ccccaagac gccttcttc 
acgtcggat acggcggaa atgaagctg gtcacgtgc 
gtcttcgaa gtgtccaaa agcatagca gaactgaaa 
cttaccgaa acagagttg gctctgtat tcggcctgc 
gtcttgctc tctccagat cggccgggg ctgaaaggt 
atcggcgaa atcagtcga cttagccaa gccgtcgtg 
agagcctta agacttgag ctagacaga aaccacagc 
atgcctata aaaggggat gttacagtt tgtgatgcc 
ttgcttaca aaaatacct acgttaagg gagataagt 
atgcttcac atggatgca ttaggaaaa ctcaaaaga 
tctgcacca cacctggag tttcccgcc cttcacaag 
gagttgttt agtgtggac agttga
    mRNA sequence
    • >TC008909 RA
      ggatacggc tacgtcacg gagccggcg ctaagacct 
acgttttta tttacatcg aatgcgtta gataaggac 
gaaatgctg gcagtcggt ctgcgctcc aaggcagac 
tttcttaaa aaatgtcca aaatatgtg ccggtttta 
cgcaagata tcgacggca aggtccgcc acggcaagt 
gaactcgcg cagtgtccc agggattta attaaaatc 
ccacttgag cacagtggc tgttttttc tcagtaaat 
cattttttg atcctcggt gcagtgagt gacagttac 
ctgcaataa cggctcgag tgacgtttg tgcttttgt 
gtggatttt ccgggaaaa tttcggatt cgaaggcag 
tgttttgca atattgcac gctccgaga tagcgtata 
aaatagcag aagtgaaac tctccgtcc tgagccgag 
taattatga ttttggggt tgacgattg gcaccactg 
tccgcgggg cgacgtctg atccaggga aaaaattcc 
gcgtgcact taaatttta atgattatt gataataat 
gttgttgtt ttatcgtaa tcggtcgtt cacattcgc 
gtaaaaata gacgagtgc ttatgcgca accgacttt 
gtcttgaag gcgattttt ccgttggtg gtcattttt 
cggaattct cccgtatta acgatcgca tctctcgct 
caaccatat tcggcggtg aacaaccta gagttcaat 
ggcttgccg cgcctccaa acacaaacc gtcgtactt 
tatgccggt ttgagccaa acaaaccgt attaatggg 
ccgcccttt cgaatttcc ctcattccg gcactgcag 
catccccga gctggcagc cctggcgtg atttttcga 
aatttccga cgccgtgcg cctcgaggt caacgaccc 
cgcgcgcag ccagatttt actttgttg aatttacca 
atgacccct tgtacggcg ccttatcac actcctact 
gagtaaaga ctcatgtat gagccatcc caaattgat 
tagcaccga cacaggttg ggcgaagag aatggagcc 
gaaagttgg tgcttcggc ggctcattc tgatgagta 
aaaacgtga tcgatctcc ctcgttcgt gtttttctc 
agtttcagg tgtctgacg atggaaatc gacgtcgtc 
aatatcgac cttaaacta gtccatcga tcttgcgtg 
aaataaaag tggttcaaa gtttgtgcg agtggcgaa 
ttgtggctg cggccatgc gacgcactt ctggatttg 
ccattaaaa tacgaggtg ccagtgatc gaacgttag 
tgtttttgt ttttgtaca atggacaat tttcaagag 
ttgttcagt ggtggggaa acttggccg actgactca 
agggatatt agaccggat aatatttcc tcgacggac 
tcagcaacc ccaccccct caaatcgac gacaaggat 
gacaagaaa agcgccaat tccatcaga gcacaaatc 
gagataatc ccgtgcaaa gtatgtggg gacaagagc 
agcggtgtt cactatggt gtgattaca tgcgaggga 
tgtaagggg tttttccga agatcacag agttcagtt 
gtaaactat cagtgtcct agaaataaa aactgtgtt 
gtcgacagg gtaaatagg aatcgatgt caatactgc 
cgactgcag aaatgtctt cggctggga atgagccga 
gatgctgtc aaattcggc cgaatgtcg aagaagcag 
cgagagaaa gtagaagac gaagtcaga tttcatagg 
gcacagtta agggcgcag agcgattct gctcctgat 
agttcagtg ttcgaacaa caaacaccc tcaagtagt 
gatcagtta catcatagt tacaatggc gggtatacg 
tacaacagc gatgtcagc gcgtacact ccagcctat 
tacacgaat acaacccaa gtccaagcg aattcgatg 
ggctatgac atctccgca gattacgtg gacagtaca 
acagcgtat gatccgagg ccgggcctg gacacactg 
aacgacacg ggaagctta atggccaac gttgtaaca 
acagatcca agtcagata tcggagtta ttatcgaag 
acaatatca gacgcacac acacgtact tgtttatat 
tccctcgaa catattcac gagatgttc aggaaaccc 
catgatctc tcccgactt ttgtactat aagaacatg 
gcccatgaa gagttgtgg ctcgaatgc gcccaaaaa 
ctcacaaca atcatccag caaatcatc gaatttgcg 
aaaatggta cctggattc atgaaattg tcccaagac 
gatcagatc gtattattg aaagcaggt agttttgaa 
ctggcaatt attagaatg tcacgttat ttggatctg 
tcccaaaac tgtgttctc tacggcgat acgatgctc 
ccccaagac gccttcttc acgtcggat acggcggaa 
atgaagctg gtcacgtgc gtcttcgaa gtgtccaaa 
agcatagca gaactgaaa cttaccgaa acagagttg 
gctctgtat tcggcctgc gtcttgctc tctccagat 
cggccgggg ctgaaaggt atcggcgaa atcagtcga 
cttagccaa gccgtcgtg agagcctta agacttgag 
ctagacaga aaccacagc atgcctata aaaggggat 
gttacagtt tgtgatgcc ttgcttaca aaaatacct 
acgttaagg gagataagt atgcttcac atggatgca 
ttaggaaaa ctcaaaaga tctgcacca cacctggag 
tttcccgcc cttcacaag gagttgttt agtgtggac 
agttgaaaa acttcagca gaatcttct gatccgtag 
caacatagc tactgtaag tatttctca attactgga 
aagaaacca caaaacgtt acgtccaaa taacaaaat 
tttacggat ttgtttgtt gcagattcc ttcatactg 
aatgtatcg gccttaaat acataaata taaagtata 
atttcgtta taatgagat aaaatttat tgttgtgac 
atagaaata aagaaccgc agatctcta agtattatt 
taaaaaact gcaaaagct ctatgaagc cgggccctg 
atttcaaag tacaactta ttgtttgtt gtttgttat 
tatatctac gcagtgtgt tattatatg gttctgaat 
cctaggtgc tgaataaaa tcgattggt atatttttg 
cggtttttt aaatgaaat atttacgta tcgtaatct 
gccacttaa cgtaacacg ctaattaca cgtaagatt 
aaagttata cactattga tatataata tatgaccaa 
caagttata agtcagtga aactcttgt ttagtttta 
cgtatttaa agaaaaaat catacttag actgcgttt 
atattataa taatacgta agtatactg aaatcactt 
tagttggta gtgttgaaa tagataaca tacaataca 
ttataaata tagacaaga ctgtatgct tacactgta 
gagttttta actggtttt taacttgtt gttttttag 
ttaagtttt acttggtga cacatggtt ttataaaaa 
tatatataa aagagatgt atatgtata aagtatatg 
aaatagtat atagagtat ttatgaaat agaactata 
tctgattct tgttttacg gattttgtt ttattatgg 
attcgaaca taatttttt ttccataat aaaatgaaa 
attttgcga tcgtgataa cagactttt ttcagcttt 
tacaattta tagatttgt tagatttgc aaggaaatt 
acacgaaca cggagaaca aacttggat cgcagcagc 
tacttgcct ctcttcatt tttctcggt attaatgtc 
aaaagttaa gtatgttta tgtaatttc ccgtcgaaa 
atgccagaa tgaatagtt ctaattttt aagttagtt 
ttaacgtgc ttgtacatg ttaaccatt gttaaatct 
cattttaac ttagtactt tcaacccag tgttataaa 
gctatttat gtaattaga atctttgta ggtatgaaa 
caaacctaa catcacatt ttttttaaa caaaaactc 
aaaggtctt gtattatag aaaattttt gtattttaa 
taataattg tagtttctt ccgttttga caatggtta 
acatccatg agaggctat tggttattt ttgtattta 
acgaaccat gtaatttta gaaataact attttttgg 
agattacta taaagacat ggctgtgtt acatacatg 
ataacagtt ttacgaagt gtaatttgc cttttttgt 
gttaacttt ttgttaaat aaagaaatt acaatcttc 
cacaatatt ttattttaa atataatat ttttgcaaa 
catatcact aattccgcg ctatgttgt atttctcgt 
tgacaattt atttaacgt aaagttaac aaatttgcg 
agttgcaag tgcgtaaaa atagccact tttgtaaat 
gagggttgg cagtgggta atggtatag ctattgtta 
aatgattat tatgaacta aaatgttta aaaaacatg 
taccttggg ctacaaact gtacattat cttaattta 
ttagaatcc tttatactg ctggtgaaa tttttttag 
tgtagatgt tgctaacat tttgtttat attatatat 
attatatat attttattt ctgttggtc tatgacaat 
tattacagt ttgttaata tttttgtta gtattttat 
ttttcctcc taataatct ctttctgta gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY