Status
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Gene information
General information
TC033769
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008883,TC008884
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11300150..11305810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033769
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04558

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033769 RA
      MHAGNVLEF QLQPGVSIK PKVDENEVK NILSGIYGL 
      KCVSIKQLN GYDDFNFHV KVSDECDNE NIKKINKDG 
      YILKVINSL DSQRPQFFE AQNEVLRFL GKTSICCPQ 
      PVQNKSGEF YIIRTFSSG KHIVRLLEF IAGSILHQV 
      PTSVNLFYK VGKFAAQLD QALKKFHHP AYDCIKSVW 
      HLESAPQLS KFLYVITDE TRKKIVSEV IEDFPKRVL 
      AAKSRLEKG VIHGDFNEQ NILVDEKDN ELYVKAILD 
      FGDSQFGCY IYELAIAMA YMMIQGKSI EAGGYVLAG 
      YNSVRKISD EEYNLLKTC IAARMSQSL VLGVYTSIH 
      DPDNPYILT TAKTGWALL VDFWSKPES ELLEIKTMD 
      TGNVLASQL QPGVSVKPK VDENEVKNI LSSIYGLNC 
      VSIKQLNGY DDFNFHVKV SDKCDNKNI KKVNKDGYI 
      LKIINSLDS QRPQFFEAQ NEVLRFLGK TSICCPQPV 
      QSKNGEFNI IRTFSSGKH IVRLLEFIT GSILQQVPI 
      SMKLFYKVG KFAAQLDQA LKKFHHPAY DCIKSAWHL 
      ESAPQLSQL LYVITDKAK KTIISEVFE EFPKRVLAA 
      NLEKGIIHG DFNEHNILV DEKDNEFYV KGILDFGDT 
      HVSCYIFEL AITMAYLMI QGKSIEAGG YVLAGYNSV 
      RKISDEEYN LLKICIAVR LCQSLVIGA YSSMNDPDN 
      PYILISAKG VWALLSDLW PKPESELLE VWKKIVDET 
      ADWS
    CDS sequence
    • >TC033769 RA
      atgcatgca ggcaacgtg ctagagttt caactgcaa 
      cccggtgta tcaataaaa ccaaaagta gacgaaaat 
      gaagtcaaa aatatctta agcggtatt tacggcttg 
      aagtgcgtt tcgataaaa cagctcaac gggtacgac 
      gatttcaat ttccacgtt aaagtcagt gacgagtgc 
      gataatgaa aatataaag aaaataaac aaagatggt 
      tacatttta aaagttata aattctttg gattcacaa 
      agaccgcaa ttttttgaa gcccaaaat gaagttcta 
      cgtttcttg ggaaaaact tcaatttgt tgcccgcaa 
      cccgtccaa aacaaaagt ggagagttt tacatcatt 
      cggacattt tcaagtgga aaacacata gttcgcttg 
      ctggaattc atcgctggc tcaatttta caccaagtg 
      cctacttca gtgaacctg ttttataaa gttggaaaa 
      ttcgctgcg caactggat caagccctg aagaaattt 
      catcatccg gcgtacgat tgcatcaaa tctgtttgg 
      cacttagag tcagctcca cagctatca aagttcttg 
      tacgtcata actgacgag actaggaaa aaaatcgta 
      agcgaagtc atcgaagat tttccgaag cgagtttta 
      gcggctaag tcgcgtctg gagaaaggg gtaattcat 
      ggcgatttc aacgagcaa aacattttg gtggacgag 
      aaagacaac gaattgtac gttaaagcg attttagat 
      tttggtgat agccagttt ggctgttac atttatgaa 
      ctggcgatc gctatggcc tatatgatg atccaggga 
      aaaagcatc gaagctggt ggttatgtc ttagccggg 
      tataactcg gttaggaaa atttctgat gaagaatac 
      aatttatta aaaacgtgt atagctgcc cgaatgagc 
      caatctcta gttcttgga gtatataca agcattcat 
      gatcctgac aatccttat attttaaca actgcaaaa 
      actggatgg gctttactg gtcgatttt tggtctaaa 
      cctgaaagt gaattgttg gagataaaa accatggac 
      acaggcaac gtgttagcg tctcagctt caacccggt 
      gtatcagta aaaccaaaa gttgacgaa aacgaagtc 
      aaaaatata ctaagcagt atttatggc ttgaactgt 
      gtttcaata aaacagctc aacgggtac gacgacttc 
      aatttccac gttaaagtt agtgacaag tgcgataat 
      aaaaatata aagaaagta aacaaagat ggttacatt 
      ttaaaaatc ataaattct ttggattcg caaagaccg 
      caatttttt gaagcccaa aatgaagtt ctacgtttc 
      ttggggaaa acttcaatt tgctgcccg caaccggtt 
      caaagtaaa aatggagag tttaacatc attcggact 
      ttttcaagt gggaaacac atagttcgc ttgctggaa 
      ttcatcact ggttcaatt ttacagcaa gtgcccatt 
      tcaatgaaa ctgttttat aaggttgga aaattcgct 
      gcgcaactg gatcaagca ttgaagaaa tttcatcac 
      ccggcgtac gattgcatc aaatctgct tggcatttg 
      gagtcagct ccgcagtta tcacaattg ttgtatgtc 
      ataactgat aaggctaag aaaacgatt ataagcgaa 
      gtgttcgaa gaatttccg aaacgagtt ttagcggct 
      aatttggag aaagggata attcatggc gatttcaat 
      gagcataac attttggtc gacgagaaa gacaacgaa 
      ttttacgtt aaagggatt ttagatttt ggcgatact 
      cacgttagt tgttacatt tttgagctg gcaattact 
      atggcttat ttgatgatc cagggaaaa agcatcgaa 
      gctggtggt tatgtgtta gcagggtat aactcggtt 
      agaaaaatt tctgatgaa gagtacaat ttgttgaag 
      atttgtata gcagtccga ttgtgccaa tctctagtt 
      attggagcg tattcaagc atgaacgat cctgacaac 
      ccttatatt ttaataagt gcaaaagga gtatgggct 
      ttactctcc gatctttgg cctaagcct gaaagtgaa 
      ttgttggag gtctggaag aaaattgtt gacgaaact 
      gctgattgg tcttag
    mRNA sequence
    • >TC033769 RA
      cacggtatt ttgtaacag cgtttgcgt agataaaaa 
      ccatgcatg caggcaacg tgctagagt ttcaactgc 
      aacccggtg tatcaataa aaccaaaag tagacgaaa 
      atgaagtca aaaatatct taagcggta tttacggct 
      tgaagtgcg tttcgataa aacagctca acgggtacg 
      acgatttca atttccacg ttaaagtca gtgacgagt 
      gcgataatg aaaatataa agaaaataa acaaagatg 
      gttacattt taaaagtta taaattctt tggattcac 
      aaagaccgc aattttttg aagcccaaa atgaagttc 
      tacgtttct tgggaaaaa cttcaattt gttgcccgc 
      aacccgtcc aaaacaaaa gtggagagt tttacatca 
      ttcggacat tttcaagtg gaaaacaca tagttcgct 
      tgctggaat tcatcgctg gctcaattt tacaccaag 
      tgcctactt cagtgaacc tgttttata aagttggaa 
      aattcgctg cgcaactgg atcaagccc tgaagaaat 
      ttcatcatc cggcgtacg attgcatca aatctgttt 
      ggcacttag agtcagctc cacagctat caaagttct 
      tgtacgtca taactgacg agactagga aaaaaatcg 
      taagcgaag tcatcgaag attttccga agcgagttt 
      tagcggcta agtcgcgtc tggagaaag gggtaattc 
      atggcgatt tcaacgagc aaaacattt tggtggacg 
      agaaagaca acgaattgt acgttaaag cgattttag 
      attttggtg atagccagt ttggctgtt acatttatg 
      aactggcga tcgctatgg cctatatga tgatccagg 
      gaaaaagca tcgaagctg gtggttatg tcttagccg 
      ggtataact cggttagga aaatttctg atgaagaat 
      acaatttat taaaaacgt gtatagctg cccgaatga 
      gccaatctc tagttcttg gagtatata caagcattc 
      atgatcctg acaatcctt atattttaa caactgcaa 
      aaactggat gggctttac tggtcgatt tttggtcta 
      aacctgaaa gtgaattgt tggagataa aaaccatgg 
      acacaggca acgtgttag cgtctcagc ttcaacccg 
      gtgtatcag taaaaccaa aagttgacg aaaacgaag 
      tcaaaaata tactaagca gtatttatg gcttgaact 
      gtgtttcaa taaaacagc tcaacgggt acgacgact 
      tcaatttcc acgttaaag ttagtgaca agtgcgata 
      ataaaaata taaagaaag taaacaaag atggttaca 
      ttttaaaaa tcataaatt ctttggatt cgcaaagac 
      cgcaatttt ttgaagccc aaaatgaag ttctacgtt 
      tcttgggga aaacttcaa tttgctgcc cgcaaccgg 
      ttcaaagta aaaatggag agtttaaca tcattcgga 
      ctttttcaa gtgggaaac acatagttc gcttgctgg 
      aattcatca ctggttcaa ttttacagc aagtgccca 
      tttcaatga aactgtttt ataaggttg gaaaattcg 
      ctgcgcaac tggatcaag cattgaaga aatttcatc 
      acccggcgt acgattgca tcaaatctg cttggcatt 
      tggagtcag ctccgcagt tatcacaat tgttgtatg 
      tcataactg ataaggcta agaaaacga ttataagcg 
      aagtgttcg aagaatttc cgaaacgag ttttagcgg 
      ctaatttgg agaaaggga taattcatg gcgatttca 
      atgagcata acattttgg tcgacgaga aagacaacg 
      aattttacg ttaaaggga ttttagatt ttggcgata 
      ctcacgtta gttgttaca tttttgagc tggcaatta 
      ctatggctt atttgatga tccagggaa aaagcatcg 
      aagctggtg gttatgtgt tagcagggt ataactcgg 
      ttagaaaaa tttctgatg aagagtaca atttgttga 
      agatttgta tagcagtcc gattgtgcc aatctctag 
      ttattggag cgtattcaa gcatgaacg atcctgaca 
      acccttata ttttaataa gtgcaaaag gagtatggg 
      ctttactct ccgatcttt ggcctaagc ctgaaagtg 
      aattgttgg aggtctgga agaaaattg ttgacgaaa 
      ctgctgatt ggtcttagc agtgaaaca tattgttta 
      aattgtaaa taaagtagc acacaagtt aaaagaaaa 
      aactttatt cgtcatttt cgcacaaac aatgtttta 
      aattttagt ttaaaagca acgttttaa gtagattcc 
      ttgggaaaa tttaaaatg gaatgatta cgtaaggaa 
      tacaaatga ataactaac ctaagagat aatacgata 
      ataatagta acaataaaa attgctata aatttctcc 
      actgaatcc tattttatt atagtggta gttaatatt 
      tgaaatata actcttgaa ctttagcta aacgtaagc 
      agtggcgta tcggaaggg gcagaagtg gatttaatc 
      ctagatatg ccactggaa acgagcgtt tctgctaat 
      aaatttcga tcaccgtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY