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Gene information
General information
TC008876 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11267500..11276286
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008876
    iBeetle screen iB_04557

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen posterior thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008876 RA
      MSCNSNPTS TCSSASNCN DMDSLVCNI ISVALQDIC 
      GNCDEGAKA TSRCIDCSE LLCDPCVRA HQRVRVTKD 
      HRINKFSNN LVSSPTTID SAVCSPETR NSPCAAINT 
      ICDIHREPN RLFCATCYT PACSDCVLS DHVSHELSY 
      VQECDNTIL MHTKLNNET RSAMTAVAE AMENVKFMS 
      TKIHLKLQK TTVDVQNVF KKYVTALER RAQELVSKI 
      EQIQRFKAY RLLSQMERL RLAQARLTY VSNILNASF 
      ASATNSEFI IANEKAYNE LKSIRSVQA ELVPCEDDY 
      IRFTAVDNG LFSAINSLG NINTSSAMS WGPEPSTPL 
      ATEILKMSD INQNCRPLY GVVDQVSVK HVGSASPMI 
      FAKEGVEDG QLCRPWGVC CNSLGHIIV TDRSNNRIQ 
      VFDSNGKFL YKFGKQGSG LGQFERPAG ITVSPVNHI 
      VVADKDNHR IQIFKMDGT FILTFGEKG TQNGQFNYP 
      WDVACNSQG DIVVSDTRN HRIQLFTGA GAFINKYGF 
      EGNSSMWKF FDCPRGVCF TPAGNVMVT DFNNHRIVI 
      IDKDFVRAQ FLGEEGSKD KQFLRPQGI ICDDQGNIV 
      VADSKNHRI QVFDSFGNF LFQLGRPGK GPGEFDRPS 
      GLCLSPTGR IIVVDFANA RVQVF
    CDS sequence
    • >TC008876 RA
      atgtcttgc aacagtaac ccaacatcc acgtgctcg 
      tctgcatcg aactgcaac gacatggac agtttggtc 
      tgcaacatc atatcggtg gctttgcaa gacatatgt 
      gggaactgc gatgaagga gccaaagca acatcaaga 
      tgcatcgac tgcagcgaa cttttatgc gacccttgc 
      gttagagcc caccaacgc gtacgtgta accaaagac 
      caccgaatt aacaaattt tcgaataat cttgtctca 
      tcacccacg actatcgac tcagcggtc tgctcgccc 
      gagactagg aacagtcct tgcgccgca ataaacaca 
      atctgtgat attcatcgg gagcctaat aggctcttt 
      tgtgcaaca tgttatact ccagcctgc agcgactgt 
      gtcctaagc gaccacgtt tcgcacgag ttatcctat 
      gtccaagaa tgcgataat acgattcta atgcatacc 
      aaattaaat aatgaaacc agaagtgca atgacggca 
      gttgcagaa gcaatggaa aatgtcaag tttatgtcc 
      accaaaatt cacctcaag ctgcaaaag acgacggtg 
      gatgtgcag aacgtcttt aaaaaatac gtcacagct 
      ttagaacgt cgcgcccaa gagctcgtg agcaaaatc 
      gagcaaatc cagcggttc aaagcctac cgcctcctc 
      tcgcaaatg gagcgcctc cgcctggcc caagcccgc 
      ctcacctac gtctccaac atcctcaac gcgtccttc 
      gccagcgcc accaacagc gaattcatc atcgccaac 
      gaaaaggcc tacaacgag ctcaaatcc atccggtcc 
      gttcaggcc gaactcgtc ccatgcgaa gacgactac 
      atccgcttc acggccgtc gacaacggc ctcttctcc 
      gccatcaac tccctgggc aacatcaac accagctcg 
      gccatgagc tggggcccc gagccctcc acacccctg 
      gcaaccgag attttgaaa atgtccgac atcaaccaa 
      aactgccgg ccgctgtac ggcgtcgtc gaccaagtg 
      tcggtcaag cacgtcggc tcagcctcc cccatgatt 
      ttcgcaaag gaaggggtg gaggacggg cagttgtgc 
      cgcccctgg ggggtttgt tgcaacagc ctggggcac 
      attatagtc acagatcgg agcaacaac cggattcaa 
      gttttcgac tcgaatggc aaatttttg tacaagttt 
      ggcaaacaa gggagtggt ttgggccag tttgaacgc 
      ccggctggg atcaccgtg agccccgtt aaccacatc 
      gtagtggcc gataaagac aaccaccgc atccagatc 
      ttcaaaatg gacggtacc tttatcctg acttttggg 
      gaaaaaggg acgcaaaat gggcaattc aactaccca 
      tgggacgtt gcgtgcaac tcccaaggc gatatcgtg 
      gtctccgac acccgcaac caccgcata caactgttt 
      acgggggct ggggccttt attaacaag tacggcttc 
      gaaggcaac tcctccatg tggaagttc ttcgattgt 
      ccgcgcggg gtttgcttc actccagcc ggcaacgtc 
      atggtgacc gacttcaac aaccaccgc atcgttatc 
      attgacaag gacttcgtc cgggcgcag tttctgggc 
      gaggagggg tcgaaggac aagcagttt ttgcgcccc 
      cagggaatt atttgcgac gatcagggg aatatcgtt 
      gttgccgat tcgaaaaat caccggatt caggtgttt 
      gacagtttc gggaacttc ttgttccag ctgggaagg 
      ccggggaag ggccccggg gagtttgac aggcccagt 
      ggcctttgc ctcagcccg acagggcgc ataatcgtc 
      gtggatttt gccaacgca agggttcag gttttttga
    mRNA sequence
    • >TC008876 RA
      aaaatgctc aagaggtgt gtcaaaatc gttcttttc 
      ccgtatttg taaaccatt agcagtgta tttatgcga 
      actgcgcat ggtggtgaa gggagggat atgcaaccc 
      ctagttggg caagggaag ggcagaaag tgcacgaaa 
      ttcctgata tccaaggaa ggtcgcagt acccacacc 
      gcacagaag tgagataag tggtgttaa aaatattga 
      agtgtgtca acaacaaac ggccagttt atggaatta 
      tccgaagtc cattaggaa gtcctcacc agtcccagg 
      atgtcttgc aacagtaac ccaacatcc acgtgctcg 
      tctgcatcg aactgcaac gacatggac agtttggtc 
      tgcaacatc atatcggtg gctttgcaa gacatatgt 
      gggaactgc gatgaagga gccaaagca acatcaaga 
      tgcatcgac tgcagcgaa cttttatgc gacccttgc 
      gttagagcc caccaacgc gtacgtgta accaaagac 
      caccgaatt aacaaattt tcgaataat cttgtctca 
      tcacccacg actatcgac tcagcggtc tgctcgccc 
      gagactagg aacagtcct tgcgccgca ataaacaca 
      atctgtgat attcatcgg gagcctaat aggctcttt 
      tgtgcaaca tgttatact ccagcctgc agcgactgt 
      gtcctaagc gaccacgtt tcgcacgag ttatcctat 
      gtccaagaa tgcgataat acgattcta atgcatacc 
      aaattaaat aatgaaacc agaagtgca atgacggca 
      gttgcagaa gcaatggaa aatgtcaag tttatgtcc 
      accaaaatt cacctcaag ctgcaaaag acgacggtg 
      gatgtgcag aacgtcttt aaaaaatac gtcacagct 
      ttagaacgt cgcgcccaa gagctcgtg agcaaaatc 
      gagcaaatc cagcggttc aaagcctac cgcctcctc 
      tcgcaaatg gagcgcctc cgcctggcc caagcccgc 
      ctcacctac gtctccaac atcctcaac gcgtccttc 
      gccagcgcc accaacagc gaattcatc atcgccaac 
      gaaaaggcc tacaacgag ctcaaatcc atccggtcc 
      gttcaggcc gaactcgtc ccatgcgaa gacgactac 
      atccgcttc acggccgtc gacaacggc ctcttctcc 
      gccatcaac tccctgggc aacatcaac accagctcg 
      gccatgagc tggggcccc gagccctcc acacccctg 
      gcaaccgag attttgaaa atgtccgac atcaaccaa 
      aactgccgg ccgctgtac ggcgtcgtc gaccaagtg 
      tcggtcaag cacgtcggc tcagcctcc cccatgatt 
      ttcgcaaag gaaggggtg gaggacggg cagttgtgc 
      cgcccctgg ggggtttgt tgcaacagc ctggggcac 
      attatagtc acagatcgg agcaacaac cggattcaa 
      gttttcgac tcgaatggc aaatttttg tacaagttt 
      ggcaaacaa gggagtggt ttgggccag tttgaacgc 
      ccggctggg atcaccgtg agccccgtt aaccacatc 
      gtagtggcc gataaagac aaccaccgc atccagatc 
      ttcaaaatg gacggtacc tttatcctg acttttggg 
      gaaaaaggg acgcaaaat gggcaattc aactaccca 
      tgggacgtt gcgtgcaac tcccaaggc gatatcgtg 
      gtctccgac acccgcaac caccgcata caactgttt 
      acgggggct ggggccttt attaacaag tacggcttc 
      gaaggcaac tcctccatg tggaagttc ttcgattgt 
      ccgcgcggg gtttgcttc actccagcc ggcaacgtc 
      atggtgacc gacttcaac aaccaccgc atcgttatc 
      attgacaag gacttcgtc cgggcgcag tttctgggc 
      gaggagggg tcgaaggac aagcagttt ttgcgcccc 
      cagggaatt atttgcgac gatcagggg aatatcgtt 
      gttgccgat tcgaaaaat caccggatt caggtgttt 
      gacagtttc gggaacttc ttgttccag ctgggaagg 
      ccggggaag ggccccggg gagtttgac aggcccagt 
      ggcctttgc ctcagcccg acagggcgc ataatcgtc 
      gtggatttt gccaacgca agggttcag gttttttga 
      gacgcgctc atagattag ggaattgac tcagggttt 
      gttgccaca aagagaaac tcctcaggt aataactga 
      cctcgtgta gttaaattt aacgaattt attttattt 
      ggttgatta ggatcattg taaatatgt tattagcaa 
      ttgacaatt caggacctg taagttacc aattaagac 
      tgaaacaag actgattgt gaataggat cctattttt 
      gctaggggc gcctttgat aattagaga gccggtgtt 
      gcgattagc gtttaactg atatttact cgtgtagcc 
      ttgtagtag gaaaggaat attttgcta agcaatgtt 
      cgaacgtta cctggtgtt gtccgagcg ggcaacctt 
      taattagtc gccatttct actgtgtta agtaaaagt 
      gtggcataa catgcttat tttaccatg tttaaattg 
      attttgttg tattatgaa tgttttaat tattttaaa 
      ctatctgtg gttctccgt cagcaacta taaagataa 
      aatatgctg gtggagcgt ttataatca tcagccgat 
      gatgcaaat aacgaataa ttttagtat tattaggtt 
      aaaaacttg aaaagtatt aacactgaa cgaatattt 
      ttcactctg actgactac ttctaaaat ttgatatta 
      acttagtta ttaaggttt cgagtccat cacatgatg 
      accgaagtt aagtacttc gtgaacggt catgtgagc 
      atgcttatt tttatttag gtgttacta atgatttcg 
      ttcttctgc agcgttagt ttttgtacg ttttgtttc 
      cgccagtga tgtgacact ctttgcgca aaccaattt 
      atttttctt gacgccaga gacgtatca catcatgcg 
      gcttttgtt taggtactt tcgattgtg tcttatgtt 
      ttgttttcc ttttaatat ttttattgc aataattgt 
      gcagcgttt ccggtggaa acttgccaa aaatattca 
      tagggctgt tacgacaag gccatacgg caagtcctt 
      catttgatt ttgatttgt attagttta atacacctt 
      tttatgtgg tgcaattta agtaatata cagttccta 
      tcgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY