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Gene information
General information
TC008861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10953737..10957341
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008861
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04555

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008861 RA
      MSPVCIVLL VFVSIPCEG REGVGGSDQ DWGYITVRP 
      NAHMFWWLY YTTYNNVSN YTDRSLIIW LQGGPGMPA 
      SGFGNFLEI GPLDANFQG REGSFIENF NVLFIDNPV 
      GVGHGGETA EDLYNFLKI FLDETHPEF KNVPLYVFG 
      ESYGGKMAT EFVYRLKQK EKFENLTLC NLKALALGG 
      AFISPISYI SNYAAIAFH LGLVNKRVS ARIDELTKQ 
      IQVLINKEA YEEASDIYD NILNELDLI LNVDFHNIV 
      RKINATWMT DFQKITSTL EHVMNSKVK DKLNITADV 
      YWEYNSDVY NSIKGDVLK SVTDKIETI LNTTDVKVL 
      VYNGMLDFI VNTAGTVTW LDDLNWTGK EKWSTQNET 
      VFKINDVIE GYSKRVDNL VFYVVFRAG HSVPADNLL 
      AMNEILRKE ILDDL
    CDS sequence
    • >TC008861 RA
      atgtctcct gtgtgtatt gtcctcctg gtgttcgtt 
      tccattccg tgcgaaggt cgtgaaggg gtcgggggc 
      tctgaccaa gactggggt tacataacg gtgcgtcca 
      aatgcccat atgttttgg tggttgtat tacaccacg 
      tataataat gtttccaat tacactgac agatcatta 
      atcatttgg ctgcaaggc gggcctgga atgccagcc 
      agcggtttt gggaatttt ctcgaaata ggccctctt 
      gacgcgaat tttcagggg cgggagggg tcatttatc 
      gaaaatttt aatgttttg tttattgat aatccagtg 
      ggggtgggg catggaggc gaaaccgct gaggattta 
      tacaatttc ttgaaaatt tttctcgac gagacgcat 
      ccggaattt aaaaatgtg cctctttac gtttttggg 
      gaatcatac ggcggaaaa atggcaact gaatttgtt 
      tacagattg aaacagaag gaaaaattt gaaaattta 
      actttgtgc aatttgaaa gcacttgcg ctgggcggc 
      gctttcata tctccgatc agttacata agtaactac 
      gctgctata gcctttcat ttgggatta gtcaataag 
      cgggtttcc gcacgaatt gatgaattg actaaacaa 
      attcaagtt ttaattaac aaagaggcg tatgaggag 
      gcatcggat atttatgac aacatcctg aacgagttg 
      gatctcatt ctgaatgtg gatttccat aacattgtg 
      cgcaaaatt aacgcgact tggatgacc gactttcaa 
      aaaattaca agcacgttg gaacatgtc atgaatagt 
      aaagtcaaa gacaaacta aacattaca gcagacgtt 
      tattgggag tacaactca gacgtctac aattccata 
      aaaggagat gtactgaaa agtgtcacg gacaaaatt 
      gagacgatt cttaacaca actgacgta aaagttctt 
      gtttataac ggaatgctt gacttcata gtaaatacc 
      gcaggcacg gtgacttgg ttagacgat ttaaactgg 
      acagggaaa gaaaagtgg tcaactcag aacgagacc 
      gtgtttaaa ataaatgat gtaattgaa gggtactcc 
      aagagggtt gataattta gtgttttat gtagttttc 
      agggctgga catagtgtg cctgcagat aatttgctt 
      gcaatgaat gaaatcttg cgcaaggag atattggat 
      gatttgtaa
    mRNA sequence
    • >TC008861 RA
      taatatgcc catttatct attaattaa ccagctttt 
      gttgtcatt actaaaaaa tgtctcctg tgtgtattg 
      tcctcctgg tgttcgttt ccattccgt gcgaaggtc 
      gtgaagggg tcgggggct ctgaccaag actggggtt 
      acataacgg tgcgtccaa atgcccata tgttttggt 
      ggttgtatt acaccacgt ataataatg tttccaatt 
      acactgaca gatcattaa tcatttggc tgcaaggcg 
      ggcctggaa tgccagcca gcggttttg ggaattttc 
      tcgaaatag gccctcttg acgcgaatt ttcaggggc 
      gggaggggt catttatcg aaaatttta atgttttgt 
      ttattgata atccagtgg gggtggggc atggaggcg 
      aaaccgctg aggatttat acaatttct tgaaaattt 
      ttctcgacg agacgcatc cggaattta aaaatgtgc 
      ctctttacg tttttgggg aatcatacg gcggaaaaa 
      tggcaactg aatttgttt acagattga aacagaagg 
      aaaaatttg aaaatttaa ctttgtgca atttgaaag 
      cacttgcgc tgggcggcg ctttcatat ctccgatca 
      gttacataa gtaactacg ctgctatag cctttcatt 
      tgggattag tcaataagc gggtttccg cacgaattg 
      atgaattga ctaaacaaa ttcaagttt taattaaca 
      aagaggcgt atgaggagg catcggata tttatgaca 
      acatcctga acgagttgg atctcattc tgaatgtgg 
      atttccata acattgtgc gcaaaatta acgcgactt 
      ggatgaccg actttcaaa aaattacaa gcacgttgg 
      aacatgtca tgaatagta aagtcaaag acaaactaa 
      acattacag cagacgttt attgggagt acaactcag 
      acgtctaca attccataa aaggagatg tactgaaaa 
      gtgtcacgg acaaaattg agacgattc ttaacacaa 
      ctgacgtaa aagttcttg tttataacg gaatgcttg 
      acttcatag taaataccg caggcacgg tgacttggt 
      tagacgatt taaactgga cagggaaag aaaagtggt 
      caactcaga acgagaccg tgtttaaaa taaatgatg 
      taattgaag ggtactcca agagggttg ataatttag 
      tgttttatg tagttttca gggctggac atagtgtgc 
      ctgcagata atttgcttg caatgaatg aaatcttgc 
      gcaaggaga tattggatg atttgtaaa tgattattc 
      aatgctccg tcttaggca aagtaggta aattgtttc 
      aacgatgct tagaataga cgccccaat gaacagtcc 
      tgctgttcc tccaattga tactaaagt tcttgtaaa 
      ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY