| General information | |||
| TC008856 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10851917..10904517 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008856 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04553 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC008856 RA
MKSSFITHL KSGKSIGHT DVRSMFEVK KCSRNVEKK FIVVQTGGD GESSITEGL RTALARLRQ RSTPPGSQT SPLHQPSNC IASESCSSG STNNSHCPS SPRPKMTVN SQSGPRSPR PPDDLISTA TSALRRLHF KTGRNATKN STKQPVPKV IVMGSSTAS ETTINTSTD SSNTIVTTV TATDGDVTE DSLDHPEAV DYFNSENAD GNKILQPVP LSPKTPITA KQQTPPTAK QETRFNFDV ETPKAVLSK EENKFVFES QEPSNPQEK TVEKLMEEQ IEALKMEAR RRNSYKQAA QNDFEKMSV DPPAEKPHP KDFRRSYRA AQNDMEKMM VDKTSPKEH RRSVRKRLD VEGKEKPER TSPKRRAER TLNSSPETN DDSPKPKHR HKKASRRHH SPNKPREKF SYDEKHIAK YREKARMTP PLPDLRVDF FNETVDACQ VDSLHVSGE KRGSVCLNK CLREVGAQL EASATSPIN VTTNPLENS VHFGGRKAP QATIVVQQP SLSLDHSNV STILLKNGS EFVSNVESV GNKLSFKRQ KEENMKQLL DVANNLTLE EIHDFEMRY GSPHHNRSQ SVKTPGRSS GRPNYLCLP QQRSRVASM PNTGVEEEY YRLRHFSIT GKGVVNRGD SLKSRRSRS NNSVASSNS STEQLPGAV STAGSARTS ASCSLASSR ESSTSAPGP TPYKVLMLG GPAVGKSSL VSQFMTSEY LHAYDTSID DESGEKSVS VLLAGEESE LTFIDFATA DLSPDSCIN KYTDPHAYC VVYSSADRS SLACAEKIL QTLWTLDTI STKAVILVA NKADLVRSK VVSTEEGKS MATAYDCKY IETSVGINH NVDELLVGI LTQIRLKLE NPERSRDLF RKRSSSKKN LNRNRSPVS ATGTPTGSA ANSPKKYRG SRTSASLKV RNLLGKVWA RDSKSKSCE NLHVL
- >TC008856 RA
atgaagtcg tcttttatc acccatttg aagagcgga aaatcgatc ggtcacacc gatgtgcgt tctatgttc gaagtaaag aagtgttca agaaatgtt gagaaaaag ttcatcgtt gttcaaact ggcggtgat ggagagagc tctataacg gaggggctg agaaccgcc ctcgcccga ctgcgacag agatcaact ccccctggt agccagacg tccccattg caccaacct tctaattgc atagcctcc gagagctgc tcctcaggc agcacgaac aacagccac tgcccttct agcccgcgc cctaaaatg accgtcaat tcgcagtcg ggccccaga tctccccga cctcccgac gacctcatt agcactgcc acctctgcc ctccgacgc cttcacttc aaaaccgga agaaacgcc accaaaaac tccaccaag cagcctgtc ccaaaggtg atagtcatg ggctcaagt actgcttcc gaaaccacc atcaatacc agcacagac agctctaat accatcgta acaacagta acagcaact gacggggac gtgacggag gacagctta gaccatcct gaagcggtt gattatttc aattcagaa aacgcagat ggcaacaaa atcctccaa ccagttcca ctatcaccc aaaacacca atcaccgca aaacaacaa acacctcca actgctaaa caggaaacc cgattcaat ttcgatgtt gaaacaccc aaagctgtt ctatcaaaa gaagagaac aagttcgtg ttcgaatcg caagaaccc agcaaccct caggagaaa actgtcgag aaactgatg gaagagcag attgaggct ctgaaaatg gaagcccgt cgcagaaac agctacaaa caagcggcg caaaacgac tttgaaaaa atgtcagtg gacccgccc gcagaaaag ccccacccg aaggacttc agaaggagc taccgcgcg gctcaaaac gacatggag aagatgatg gtggacaag acgagtccg aaggagcac aggcgaagt gtgcggaaa cggctggac gtggagggc aaagagaag cccgagagg acctccccg aagcggcgc gccgagcgg accttgaac tcctcgccg gagaccaac gacgactcc cccaagccc aaacaccgg cacaaaaag gccagcaga cgccaccac tcgcccaac aagccgcga gagaagttc agctacgac gagaagcac attgccaag tatcgggaa aaggctcgg atgaccccg cctttgcca gacctccgc gtagacttc ttcaacgaa acggtggat gcttgccaa gtggattcg ttgcatgtt tccggcgaa aagcgcggc agcgtgtgt ctcaacaaa tgtttacga gaagtgggg gctcagcta gaagcatct gccacatca cctataaat gttactact aatcctttg gaaaattca gtacacttt ggtggccga aaagctccg caagccact atcgtcgtt cagcagccg tcactcagc ttggatcac tcgaatgtc tcgacgatc ctgctgaag aacggaagt gaattcgtg tcgaacgta gaaagtgtc gggaataag ttgagtttc aagagacag aaggaagaa aacatgaag cagctactg gacgtggct aacaattta acgctagaa gaaatccac gattttgag atgaggtac gggagccct caccacaat cgatctcaa tctgtaaag actccaggc cggtcttct ggtagaccg aattatctc tgtttgcca cagcaaagg tctcgagtg gcgtcgatg cccaacacg ggcgttgaa gaggagtat tatcgccta cgacacttc tcgatcact ggaaaaggc gtcgtcaat aggggcgac agtctgaaa agccgcaga tctaggtcc aacaattcg gtcgcttcc agcaattcg agcacagag cagcttccg ggggcggtg tcgacggcg ggatcggca cgtacgtcc gcttcctgc agcctggcg tcctcgcgg gagtcgtcc accagcgcc cccggcccc accccgtac aaggtcctg atgctgggg ggcccagcc gtgggcaaa tcctcccta gtctcccag tttatgact tccgagtac ctccacgct tatgacact agcatagat gacgaaagc ggggagaaa tccgtgtcc gttctgttg gccggcgag gaatccgaa cttactttt atagatttt gcgacagct gacttatcg ccggacagt tgtataaac aaatacacg gatcctcac gcatattgt gtcgtctat tcgagtgcc gaccggtcg agtcttgcg tgtgccgaa aaaatcctg cagaccctg tggacgctg gacaccatc agcacaaaa gccgtcatt ctcgtcgcg aataaagct gatcttgtc aggtcgaaa gtcgtctcg actgaagag ggaaaatcg atggcaaca gcttacgat tgcaagtac atagagaca tctgtaggc atcaatcac aacgtagac gaactgctg gtaggaatc ctcactcaa atccggtta aaattggag aatccggag agatcaagg gatctgttc cgtaagagg agctcgtcg aagaagaat ttgaatagg aatagatcg ccggtttcc gccacggga acgccgacg ggaagtgcc gccaattcg ccgaagaag tacagaggc tctcgcacc tctgcgagc ttaaaagtc cggaatttg ttagggaaa gtttgggcg agggacagc aaatccaaa tcttgtgaa aatttgcac gtcttgtaa
- >TC008856 RA
attgtttat ttaattgag atgcgagaa taattaatt tgattgtta caaggaacg acgtgatga ttggtcgag acgctttag ttaatttga tacctacac cagtaacac ccaattaat cacattacc aaaaattaa tcttcatta cacgttgac acagttttt ttcagctcc agggcttta acgaacttt tacatcaaa tttctaaat gtcggccat ttacaaaat taaaaaact ttttcattt aaaactaat aaaacgaaa tttgctacc ttcactttt actagcttg tggccaaaa tatccgcaa ttacgcaaa caatgaagt cgtctttta tcacccatt tgaagagcg gaaaatcga tcggtcaca ccgatgtgc gttctatgt tcgaagtaa agaagtgtt caagaaatg ttgagaaaa agttcatcg ttgttcaaa ctggcggtg atggagaga gctctataa cggaggggc tgagaaccg ccctcgccc gactgcgac agagatcaa ctccccctg gtagccaga cgtccccat tgcaccaac cttctaatt gcatagcct ccgagagct gctcctcag gcagcacga acaacagcc actgccctt ctagcccgc gccctaaaa tgaccgtca attcgcagt cgggcccca gatctcccc gacctcccg acgacctca ttagcactg ccacctctg ccctccgac gccttcact tcaaaaccg gaagaaacg ccaccaaaa actccacca agcagcctg tcccaaagg tgatagtca tgggctcaa gtactgctt ccgaaacca ccatcaata ccagcacag acagctcta ataccatcg taacaacag taacagcaa ctgacgggg acgtgacgg aggacagct tagaccatc ctgaagcgg ttgattatt tcaattcag aaaacgcag atggcaaca aaatcctcc aaccagttc cactatcac ccaaaacac caatcaccg caaaacaac aaacacctc caactgcta aacaggaaa cccgattca atttcgatg ttgaaacac ccaaagctg ttctatcaa aagaagaga acaagttcg tgttcgaat cgcaagaac ccagcaacc ctcaggaga aaactgtcg agaaactga tggaagagc agattgagg ctctgaaaa tggaagccc gtcgcagaa acagctaca aacaagcgg cgcaaaacg actttgaaa aaatgtcag tggacccgc ccgcagaaa agccccacc cgaaggact tcagaagga gctaccgcg cggctcaaa acgacatgg agaagatga tggtggaca agacgagtc cgaaggagc acaggcgaa gtgtgcgga aacggctgg acgtggagg gcaaagaga agcccgaga ggacctccc cgaagcggc gcgccgagc ggaccttga actcctcgc cggagacca acgacgact cccccaagc ccaaacacc ggcacaaaa aggccagca gacgccacc actcgccca acaagccgc gagagaagt tcagctacg acgagaagc acattgcca agtatcggg aaaaggctc ggatgaccc cgcctttgc cagacctcc gcgtagact tcttcaacg aaacggtgg atgcttgcc aagtggatt cgttgcatg tttccggcg aaaagcgcg gcagcgtgt gtctcaaca aatgtttac gagaagtgg gggctcagc tagaagcat ctgccacat cacctataa atgttacta ctaatcctt tggaaaatt cagtacact ttggtggcc gaaaagctc cgcaagcca ctatcgtcg ttcagcagc cgtcactca gcttggatc actcgaatg tctcgacga tcctgctga agaacggaa gtgaattcg tgtcgaacg tagaaagtg tcgggaata agttgagtt tcaagagac agaaggaag aaaacatga agcagctac tggacgtgg ctaacaatt taacgctag aagaaatcc acgattttg agatgaggt acgggagcc ctcaccaca atcgatctc aatctgtaa agactccag gccggtctt ctggtagac cgaattatc tctgtttgc cacagcaaa ggtctcgag tggcgtcga tgcccaaca cgggcgttg aagaggagt attatcgcc tacgacact tctcgatca ctggaaaag gcgtcgtca ataggggcg acagtctga aaagccgca gatctaggt ccaacaatt cggtcgctt ccagcaatt cgagcacag agcagcttc cgggggcgg tgtcgacgg cgggatcgg cacgtacgt ccgcttcct gcagcctgg cgtcctcgc gggagtcgt ccaccagcg cccccggcc ccaccccgt acaaggtcc tgatgctgg ggggcccag ccgtgggca aatcctccc tagtctccc agtttatga cttccgagt acctccacg cttatgaca ctagcatag atgacgaaa gcggggaga aatccgtgt ccgttctgt tggccggcg aggaatccg aacttactt ttatagatt ttgcgacag ctgacttat cgccggaca gttgtataa acaaataca cggatcctc acgcatatt gtgtcgtct attcgagtg ccgaccggt cgagtcttg cgtgtgccg aaaaaatcc tgcagaccc tgtggacgc tggacacca tcagcacaa aagccgtca ttctcgtcg cgaataaag ctgatcttg tcaggtcga aagtcgtct cgactgaag agggaaaat cgatggcaa cagcttacg attgcaagt acatagaga catctgtag gcatcaatc acaacgtag acgaactgc tggtaggaa tcctcactc aaatccggt taaaattgg agaatccgg agagatcaa gggatctgt tccgtaaga ggagctcgt cgaagaaga atttgaata ggaatagat cgccggttt ccgccacgg gaacgccga cgggaagtg ccgccaatt cgccgaaga agtacagag gctctcgca cctctgcga gcttaaaag tccggaatt tgttaggga aagtttggg cgagggaca gcaaatcca aatcttgtg aaaatttgc acgtcttgt aattctctt ttagttgag tccacgtgc tgaaagtac aattccaat tgtgatttt tttcgagat tagttgaga ttagtttta ggtgcacac tttttatac attttttga ccatactta tacctattt ctaagccat tattttata tttatatta gggtaaaat atacttgtt ttctaaaat catacattt cattttaac tgtctctta caacactaa caatttcca aaacatatc gaatctgag ttctgacgc ccgaaacat tttagtggg tccacgcaa gcaaacacc aaaaatagt acggccaaa ctgtgactt aaagtcccg gagtttgga gtaaaagct ttattttag tcgtttgtc cttgtcagt tttttgatt atttccgga attaatcaa attgggtcc atactgtag caagtaatt aggtagata ggtgtaaaa tatttgtag aaagtgtaa actattaaa gtaaaattt ttgaatttg tactttact gagcaaggt gaagcttta aaatgcgag aatttatta gttcgtaat ggcagttca gttttgttt aacacaggg tgttaaatt agatgcaac tgaaagaaa taacgtata cagtctggt ccaaaatat attcttaat tgtgacgag ggtacgggc gaaacatga ctgaagtca gttcatatc aaactatag ccgctataa tatatcact gctacacga cgtggcatt tgctcattt tggaccggc ctgtactta acaatgtaa atagaatat ttttatgga gttggaaat aatgactct aagaaattt aataattat tatgaatgt aaattcagt attagatgt aatttttgt tgcagccca tgtataaaa gtatttttg tctcgacaa attcgatca tgaaattta atgagaaat atgtaaatt tggaataaa ttcgattta tctttgatt gtaaaactt taacccttt gcacactaa atcacaaac aaatttgca tgctaatta taacttgga ttgtttggt atacataca aaaatattt ttgtacatt gatcaaaat tccattgta gatcagaga gctcaatta tttaaaatc aaaataaat cttagaaaa tttgaccaa ttttatttg tgcctttgt atagaggct tggttagag gacaaaagc attcaagga ctgaatttt gaagaaaca aattccata tccctctat atgttgatt aaataacgc attaatata atcagtatt cattattaa atagtatat gcaaaaagt atatattat acgtgtctt ttaaatttt caaacagtg tggtatctt ccattttta aaagtatgt cgtttaaat gtttagcag ctaagatat ctttgtttc tcttttaat aagtgccaa tctctcttt tccgttgtt gtataattt attcggtaa gtgttgatt tagtttagg tgttagcat aatggaatc cattattat aatccctga ttgtaatga tttattcca ttagcttga ttttcatgt tcggacaac cgggtaaaa ttactagaa aatgtagat cacgtttta attttttcg attcaaatt taaattgct ttatttcgt ttccgacat ttcttgtta acaaatttc agaaaaata tttagtgcg ttagggact tagttaaat cgtattgta ttattgtag tcacagtta tagaaagaa acataatgc acaagttgg aattttgaa gtttgtaaa tttataatc cattatttg tatcatgta atgaagttt atatcaata aaatttttg ccgtgta
