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Gene information
General information
TC008844 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10725383..10727792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008844
    iBeetle screen iB_04550

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    Pupal morphology
    wings not closed - Number of animals showing the phenotype: 9

    prothorax bristle number decreased - Number of animals showing the phenotype: 9

    abdomen gin-trap setae number decreased - Number of animals showing the phenotype: 9

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 9


    wings folding not present - Number of animals showing the phenotype: 9


    elytra size decreased - Number of animals showing the phenotype: 9


    elytra surface irregular - Number of animals showing the phenotype: 9

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008844 RA
      MPAPIEEQE IEKNEIDEV GSLSFSPPP AKLLETRDS 
      ISSIDSDVS LSFDRSPSN GENNAEAED EGAHMADLS 
      DSASDTGSA GGGKDSGCE VTPEVTEPP FQPPSDELA 
      DKIVQQVEF YFSDANITK DAFLLKHVK RNKEGYVSL 
      KLISSFKRV KHLTKDWRV VAHALSRST KLEINEAGT 
      KLRRLDPLP QYDETTPSR TVVAVHMPI EKATIENVA 
      ELFKVCGEI ALIRILRPG NPIPADVRQ FINKNPSLA 
      GVVCALVEF VTSESARNA IQLQHTLGE PMKVYELNN 
      VPHPERKKK TNKKNNNNK NYSDNDYIS SSCQSSSEA 
      EDIKNRAKL RRGSSAHFP SRYIEPAAW LQRRLSASS 
      TEANFIYMP RRLSYSSRD SSDSSLFLP RRMSACSLS 
      GSEGGFNRR LSSASQSSD YSNPRSRSN STAFQNGEN 
      VVRMPRGPD GSKGFRQRP LTAIAQ
    CDS sequence
    • >TC008844 RA
      atgccggcc ccaatcgaa gagcaagaa atcgaaaag 
      aacgaaatc gacgaagtc ggctcgttg tcgttctcg 
      ccgccgccg gccaagctc ctggagacc cgagacagc 
      atctcctcc atcgacagc gatgtttcc ttgtcgttc 
      gaccggagc cccagcaac ggcgagaac aatgcggag 
      gccgaggac gagggggcc cacatggcc gacctctcc 
      gacagcgcc tccgacacc ggctccgcc gggggcggc 
      aaagactcc ggctgtgag gtcactccc gaggtgacc 
      gaacccccg ttccagccg ccctcggac gaactcgcc 
      gataagatc gtccagcaa gtcgagttc tacttctcc 
      gacgccaac atcacgaaa gacgccttc ctcttaaaa 
      cacgtcaaa cgaaacaag gagggctac gtcagtctc 
      aagctcatc tccagcttc aagagagtc aaacacttg 
      accaaagac tggcgagtg gtggcccat gccttgtcc 
      agatccacc aaactagaa ataaacgaa gctgggacc 
      aaactaaga cggctggac cctctcccc cagtacgac 
      gaaacaacc ccctcgcgc acggtggta gccgtccat 
      atgcccatc gaaaaggcc accatcgaa aacgtggcc 
      gaactgttc aaagtctgc ggcgaaatc gccctaatc 
      cgaatccta cgacccggg aatccaatc ccagcagat 
      gttagacaa tttatcaac aagaacccg tcactggcc 
      ggggttgtg tgtgcctta gtcgaattt gtaacctcc 
      gaatcggcc cgcaacgcg atccagttg cagcacacc 
      ctcggagaa cccatgaaa gtatacgaa ctgaacaac 
      gtcccccat ccagagcgc aagaaaaag accaataag 
      aaaaataac aataataaa aactactcc gataacgac 
      tacatcagc tcgtcgtgc cagagcagc tccgaggcc 
      gaggacatc aaaaaccgg gcgaagctc cgccggggc 
      tcctctgcc cacttccct tctcgctac attgaaccc 
      gcggcgtgg ctccagcgg cgcctctcg gcctccagc 
      accgaagcc aacttcatc tacatgccc cggcgcttg 
      agctacagc agccgcgac tcgagcgac tccagcctg 
      ttcctgccg cgcaggatg agcgcctgc agcttgtcg 
      ggctccgag ggcggcttc aaccggcgg ctgtcctcc 
      gcgagccag tcgtcagat tatagcaat ccgcgctcg 
      aggagcaac agcacggcc ttccagaac ggggagaac 
      gtggtgcgg atgccacgt ggtccggat ggttccaaa 
      ggattccga cagagacct ttaacggcg atcgctcaa 
      tga
    mRNA sequence
    • >TC008844 RA
      ccagtagta cgctgaaag ctaggtcat aaaacaggt 
      gtgttgtgg cagtagcga acccgatca ccacgtgtg 
      acctacatt cacacgaca tactatccc agtgtacat 
      tttcaatgt gaaagtgcc ttaaaagaa agactgacc 
      gactaagct gcgaaaaac acatccagt gataaattg 
      tgatattag tttagtgac gcgaaatgc cggccccaa 
      tcgaagagc aagaaatcg aaaagaacg aaatcgacg 
      aagtcggct cgttgtcgt tctcgccgc cgccggcca 
      agctcctgg agacccgag acagcatct cctccatcg 
      acagcgatg tttccttgt cgttcgacc ggagcccca 
      gcaacggcg agaacaatg cggaggccg aggacgagg 
      gggcccaca tggccgacc tctccgaca gcgcctccg 
      acaccggct ccgccgggg gcggcaaag actccggct 
      gtgaggtca ctcccgagg tgaccgaac ccccgttcc 
      agccgccct cggacgaac tcgccgata agatcgtcc 
      agcaagtcg agttctact tctccgacg ccaacatca 
      cgaaagacg ccttcctct taaaacacg tcaaacgaa 
      acaaggagg gctacgtca gtctcaagc tcatctcca 
      gcttcaaga gagtcaaac acttgacca aagactggc 
      gagtggtgg cccatgcct tgtccagat ccaccaaac 
      tagaaataa acgaagctg ggaccaaac taagacggc 
      tggaccctc tcccccagt acgacgaaa caaccccct 
      cgcgcacgg tggtagccg tccatatgc ccatcgaaa 
      aggccacca tcgaaaacg tggccgaac tgttcaaag 
      tctgcggcg aaatcgccc taatccgaa tcctacgac 
      ccgggaatc caatcccag cagatgtta gacaattta 
      tcaacaaga acccgtcac tggccgggg ttgtgtgtg 
      ccttagtcg aatttgtaa cctccgaat cggcccgca 
      acgcgatcc agttgcagc acaccctcg gagaaccca 
      tgaaagtat acgaactga acaacgtcc cccatccag 
      agcgcaaga aaaagacca ataagaaaa ataacaata 
      ataaaaact actccgata acgactaca tcagctcgt 
      cgtgccaga gcagctccg aggccgagg acatcaaaa 
      accgggcga agctccgcc ggggctcct ctgcccact 
      tcccttctc gctacattg aacccgcgg cgtggctcc 
      agcggcgcc tctcggcct ccagcaccg aagccaact 
      tcatctaca tgccccggc gcttgagct acagcagcc 
      gcgactcga gcgactcca gcctgttcc tgccgcgca 
      ggatgagcg cctgcagct tgtcgggct ccgagggcg 
      gcttcaacc ggcggctgt cctccgcga gccagtcgt 
      cagattata gcaatccgc gctcgagga gcaacagca 
      cggccttcc agaacgggg agaacgtgg tgcggatgc 
      cacgtggtc cggatggtt ccaaaggat tccgacaga 
      gacctttaa cggcgatcg ctcaatgag aggttcatg 
      ataatcgga gccttgacg ttaaacttt tacttgcct 
      aacggcgga aagcggctt tttcttggc gagggaaaa 
      tttgctggt cgaaccttg cgaatgcat tattcagaa 
      cacgaccca cttactttt tgttgtttc tagcacgga 
      ctttcaccc aaatgtggt ctatattgt agtgattag 
      tattagcga aaagttaga aatttctac tcctaattg 
      aaattaatt tgttacgct ttttaattt atggaaaaa 
      aatgattgg attcttcgc aaaaccacc gagttttta 
      ttggcttag ttttgtagg ttgcgtact taatttact 
      ttctacttt gttaattaa gttaattat agatttaac 
      gcttcattt tctttccct attgttttg ttttaaaaa 
      catttagat taaggcact agctaggag aaaactgtt 
      gataatgta gaagctgtg gattttaag ctgttgatc 
      ggtcggcct tgtgtaatt tttatagaa aataattgt 
      ttactatta tgcattttg atgtggttt atgctgttt 
      ccgtgtaat ctgtaagga attttcttc gctagaatt 
      aatctctaa ttcctaagt atgtaaatt cccttttgt 
      acataagcg tatttatta tttccttat ttatttcga 
      ttacccaat ttactgttt taataatga gcgttttat 
      ttatttatt tgttgtcta ttattattt acattaaat 
      ttttgggcg tacacttgt ttttcattc attatttac 
      ctccatcca acattaaaa attcagcag acacgtgct 
      ctaaactga cacgcaata aatattgat accgata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY