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Gene information
General information
TC008840 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10683595..10685511
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008840
    iBeetle screen iB_04549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008840 RA
      MSRIFRRLF SKLDSSDLL RRKMAPPRY FPNTMPDYE 
      GQDIDTQLP QGFLLKFME KIIKDIEKA IKPIDRNGN 
      DGLYLGTAG ISYMYYHLS KIPNLNEHR NRFLTKAVE 
      YLAPAFNAM RNSSQRDVP SFILGNSGV YAVASAIYR 
      AMGDTNQSE NFRKMYHDA GNICKDMQF LNCGSDELF 
      VGRAGYVLG ALWLAKETN TELPKNEIF ILCNVMVES 
      GRKYAQRRS CPCPLMYSY YQVEYLGAA HGLCTILQA 
      ILSVPGYLD LNPAHAKEV KASVDYLLS LQDRDGNFP 
      CASDEIGSH SELVHWCHG AAGMIYLMA KAYLVWREE 
      KYLRSCEKM GDLIWAKGL LKKGPGICH GVAGNGYAF 
      LLLYRLTEQ PKHLHRAVS FAKFMQTKQ FVNQARTPD 
      NPFSLYEGI AGTACFLGD LLCPQQAVF PFSDVF
    CDS sequence
    • >TC008840 RA
      atgtcacga attttccgc cggttgttc tcaaaacta 
      gactcaagc gacttattg agacgaaaa atggcgccc 
      ccacgttac ttcccaaac acaatgccc gactacgag 
      ggccaagac atcgacact caattgccg caaggtttt 
      ttactaaaa tttatggaa aaaataata aaggacatc 
      gaaaaagca ataaaacct atcgatcgc aacggcaac 
      gatggcttg tacttgggc acagccggg atttcctac 
      atgtactac cacctaagc aaaatccca aatctgaac 
      gagcacagg aatcgtttc ctgacaaaa gcggtggag 
      tatctcgct cctgcgttc aacgcaatg aggaacagt 
      tcccaacgc gacgttcct tcgttcatt ttgggcaat 
      tcgggggtt tacgcggtc gcttcggcc atttacagg 
      gccatgggg gacaccaat cagagtgaa aacttcagg 
      aaaatgtac cacgacgct gggaacatt tgcaaagac 
      atgcagttc ctaaactgc ggctctgac gagctgttc 
      gttggacga gctggctac gtgttgggt gcgttgtgg 
      ctagccaaa gaaacaaac acagaattg ccgaaaaac 
      gagattttt attttgtgc aatgttatg gtggagtcg 
      gggcgcaag tacgcccaa aggcgcagc tgtccgtgc 
      ccccttatg tactcctac taccaggtg gagtacctg 
      ggggctgcg cacggcctt tgcactata ctacaagcc 
      atactcagc gtgccagga tacctagat ttaaaccct 
      gcacatgca aaggaagtt aaagcgtcc gtggactat 
      ctcttatcg ctgcaagac cgcgacggg aattttccc 
      tgcgctagc gacgaaata ggcagccat agcgagtta 
      gtccactgg tgtcatggg gccgctggg atgatttat 
      ctgatggct aaggcgtat ttagtgtgg cgggaggaa 
      aaatatctg aggtcgtgc gagaaaatg ggggatttg 
      atctgggct aaggggttg ttgaagaaa ggaccgggg 
      atttgtcac ggggtggca gggaatggc tatgcgttc 
      ttgttgttg tacaggttg accgaacag cccaaacat 
      ttacacaga gcggtcagc tttgcgaag ttcatgcag 
      acaaagcag tttgtaaat caagcgagg actcctgac 
      aaccccttt agcttgtat gaggggatc gcaggaacg 
      gcatgcttc ctaggggat ctgttgtgt ccgcagcaa 
      gccgtcttt cccttctct gacgtattt taa
    mRNA sequence
    • >TC008840 RA
      atccggttt gtcaactca tcacaaaac tagagaaat 
      gaaaatgtt attttgtga tttccaccg ttaatgtca 
      cgaattttc cgccggttg ttctcaaaa ctagactca 
      agcgactta ttgagacga aaaatggcg cccccacgt 
      tacttccca aacacaatg cccgactac gagggccaa 
      gacatcgac actcaattg ccgcaaggt tttttacta 
      aaatttatg gaaaaaata ataaaggac atcgaaaaa 
      gcaataaaa cctatcgat cgcaacggc aacgatggc 
      ttgtacttg ggcacagcc gggatttcc tacatgtac 
      taccaccta agcaaaatc ccaaatctg aacgagcac 
      aggaatcgt ttcctgaca aaagcggtg gagtatctc 
      gctcctgcg ttcaacgca atgaggaac agttcccaa 
      cgcgacgtt ccttcgttc attttgggc aattcgggg 
      gtttacgcg gtcgcttcg gccatttac agggccatg 
      ggggacacc aatcagagt gaaaacttc aggaaaatg 
      taccacgac gctgggaac atttgcaaa gacatgcag 
      ttcctaaac tgcggctct gacgagctg ttcgttgga 
      cgagctggc tacgtgttg ggtgcgttg tggctagcc 
      aaagaaaca aacacagaa ttgccgaaa aacgagatt 
      tttattttg tgcaatgtt atggtggag tcggggcgc 
      aagtacgcc caaaggcgc agctgtccg tgccccctt 
      atgtactcc tactaccag gtggagtac ctgggggct 
      gcgcacggc ctttgcact atactacaa gccatactc 
      agcgtgcca ggataccta gatttaaac cctgcacat 
      gcaaaggaa gttaaagcg tccgtggac tatctctta 
      tcgctgcaa gaccgcgac gggaatttt ccctgcgct 
      agcgacgaa ataggcagc catagcgag ttagtccac 
      tggtgtcat ggggccgct gggatgatt tatctgatg 
      gctaaggcg tatttagtg tggcgggag gaaaaatat 
      ctgaggtcg tgcgagaaa atgggggat ttgatctgg 
      gctaagggg ttgttgaag aaaggaccg gggatttgt 
      cacggggtg gcagggaat ggctatgcg ttcttgttg 
      ttgtacagg ttgaccgaa cagcccaaa catttacac 
      agagcggtc agctttgcg aagttcatg cagacaaag 
      cagtttgta aatcaagcg aggactcct gacaacccc 
      tttagcttg tatgagggg atcgcagga acggcatgc 
      ttcctaggg gatctgttg tgtccgcag caagccgtc 
      tttcccttc tctgacgta ttttaaacc gtattgtag 
      ctaaattat taaatctct aacacaaat agtgttttt 
      ttatctttt ccttttatc agttaaatt tatgaaatg 
      taaaccttt gtaaataca ataaacaat ttcttcctg 
      gaattttaa atacgaaat ctactaacc tgcacttga 
      aagcaatat ttcgtgtgg tgatgggac tgttaaaat 
      atcctcaat agtaacttg cggagtcgg gcgtttggg 
      aaacttata cttttcttt gttgctgtc ttaaattat 
      tttttttta atttgctca aaatacagt tatttacga 
      gtacaatac ctatgcaac attttacaa ccgctatta 
      gaataaata caaggacta tgagggcaa cgactgccc 
      aagatcgag agtcgtagt tagagacgt caggcatat 
      ttttgtaac aggattaac taattaatg attaatgaa 
      agcgctcac ttttaaggt taatagtac tgattccac 
      cagaccata caaaaataa attctattc atagtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY