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Gene information
General information
TC008826 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10515147..10517758
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008826
    iBeetle screen iB_04547

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    23 on slide

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008826 RA
      MSNTWSPQA PEWSEEEKS LLPLEIAKE SRTTLATVL 
      EKSDNFVLK FPVDSNRCK TLRTNVDND TLERNINSA 
      YPVLHANIL QFYAQFILH KRKNGNTIE KALYNDMNL 
      LQFVDRLLS KRAATFIGK QDSYTLLNG KKGRGKWET 
      IGTLAEKKP LVLKDCLSY EEVKLSAFL SVSSFTHFV 
      NLGDRKNMA KYETDRSGI QDEGIIMGI IGPRLVKNE 
      VMEYQELVI TESQNTKTK GYGQTETPT IHHLFAKLY 
      HEQCLECKE ILEKKKTNP NRYTTLRND ELLDNVLYH 
      KRLVFTMDT LFTEANQRA LEKNTTAYI HVVGIGLGV 
      WKKHPCQNK LFMDSCGQR ILNLGKQLH NVSDICFAY 
      IQHNACLSY KHGDVIPIE GHPLKGIRV HICKREPHA 
      KLTGRDEGK LLVVSYAWD GNALPGNEF WIGKLGSSG 
      DSAAASSTQ ITELHNPFI NPNVSSGNL RVVKDGKVV 
      GLEEYAAEV RRQKKRKLE N
    CDS sequence
    • >TC008826 RA
      atgtcaaac acatggtca ccacaagcg ccagaatgg 
      tcggaagaa gaaaaatca ctcttacca ctggaaatt 
      gcaaaagaa agccgcaca actttagca acagtgttg 
      gaaaaaagt gacaatttt gtcctaaaa ttcccagtt 
      gattcaaac agatgtaaa actttgcga acaaatgtt 
      gataacgac acccttgag aggaacata aattctgct 
      taccccgtt ttgcacgcg aatattctt caattttat 
      gcgcaattc attctccac aaacggaaa aatggaaat 
      actatagaa aaagctctc tacaacgac atgaatctc 
      ttgcagttc gttgataga ttattgtca aaacgtgct 
      gcaacattt atagggaaa caagacagt tacactttg 
      cttaacggg aagaaagga cgaggtaaa tgggaaact 
      atcggcact ttagcggaa aaaaagccc ctagtttta 
      aaagactgt ctcagttat gaggaagta aaactatcg 
      gctttccta tcagtcagt tctttcacc cactttgtc 
      aatcttggg gaccgcaaa aacatggct aaatacgaa 
      actgaccga agtggaatt caagacgag ggaattatt 
      atggggata attggccca agattggta aaaaacgaa 
      gtgatggag taccaggag ttggtcatc accgagtca 
      caaaacacc aaaactaag ggttacgga cagacagag 
      actccaaca attcatcac cttttcgcc aaactgtac 
      catgaacag tgtttggag tgtaaggaa atacttgag 
      aagaaaaaa accaacccg aatcgttac actacttta 
      cgaaatgac gagcttttg gacaatgtc ctctaccac 
      aaacgtctc gttttcaca atggacacg ttattcacc 
      gaagccaac caacgagct ttggagaaa aacaccacg 
      gcttacatc cacgtcgtc ggaattggt ctcggagta 
      tggaaaaaa cacccatgt cagaacaaa cttttcatg 
      gattcttgt ggccagaga attctaaat ttagggaag 
      caactgcac aacgtcagc gatatttgt tttgcgtac 
      atccaacat aatgcctgt ttgagttac aagcatggg 
      gatgttatc ccgattgaa ggacatccc ttgaaaggg 
      atcagggtt catatttgc aaacgggaa cctcacgct 
      aaactaaca gggcgagac gaaggaaaa ttgctcgtt 
      gtttcgtat gcctgggat ggaaatgcc ttaccagga 
      aacgaattc tggattggg aagttgggg agtagcggt 
      gattcagca gctgcgtcc tctacccag atcacggaa 
      cttcataat ccgtttatt aaccccaat gtaagttct 
      ggcaatttg agggtagta aaggacggt aaagtggtg 
      ggactggag gagtatgcg gccgaggtc agacgccaa 
      aagaagagg aaattggaa aattag
    mRNA sequence
    • >TC008826 RA
      ctcatcaag ataatttaa aaaagtgtg cacaacact 
      gcaagtctc aacaggttt aaattaaaa taagtacca 
      agatgtcaa acacatggt caccacaag cgccagaat 
      ggtcggaag aagaaaaat cactcttac cactggaaa 
      ttgcaaaag aaagccgca caactttag caacagtgt 
      tggaaaaaa gtgacaatt ttgtcctaa aattcccag 
      ttgattcaa acagatgta aaactttgc gaacaaatg 
      ttgataacg acacccttg agaggaaca taaattctg 
      cttaccccg ttttgcacg cgaatattc ttcaatttt 
      atgcgcaat tcattctcc acaaacgga aaaatggaa 
      atactatag aaaaagctc tctacaacg acatgaatc 
      tcttgcagt tcgttgata gattattgt caaaacgtg 
      ctgcaacat ttataggga aacaagaca gttacactt 
      tgcttaacg ggaagaaag gacgaggta aatgggaaa 
      ctatcggca ctttagcgg aaaaaaagc ccctagttt 
      taaaagact gtctcagtt atgaggaag taaaactat 
      cggctttcc tatcagtca gttctttca cccactttg 
      tcaatcttg gggaccgca aaaacatgg ctaaatacg 
      aaactgacc gaagtggaa ttcaagacg agggaatta 
      ttatgggga taattggcc caagattgg taaaaaacg 
      aagtgatgg agtaccagg agttggtca tcaccgagt 
      cacaaaaca ccaaaacta agggttacg gacagacag 
      agactccaa caattcatc accttttcg ccaaactgt 
      accatgaac agtgtttgg agtgtaagg aaatacttg 
      agaagaaaa aaaccaacc cgaatcgtt acactactt 
      tacgaaatg acgagcttt tggacaatg tcctctacc 
      acaaacgtc tcgttttca caatggaca cgttattca 
      ccgaagcca accaacgag ctttggaga aaaacacca 
      cggcttaca tccacgtcg tcggaattg gtctcggag 
      tatggaaaa aacacccat gtcagaaca aacttttca 
      tggattctt gtggccaga gaattctaa atttaggga 
      agcaactgc acaacgtca gcgatattt gttttgcgt 
      acatccaac ataatgcct gtttgagtt acaagcatg 
      gggatgtta tcccgattg aaggacatc ccttgaaag 
      ggatcaggg ttcatattt gcaaacggg aacctcacg 
      ctaaactaa cagggcgag acgaaggaa aattgctcg 
      ttgtttcgt atgcctggg atggaaatg ccttaccag 
      gaaacgaat tctggattg ggaagttgg ggagtagcg 
      gtgattcag cagctgcgt cctctaccc agatcacgg 
      aacttcata atccgttta ttaacccca atgtaagtt 
      ctggcaatt tgagggtag taaaggacg gtaaagtgg 
      tgggactgg aggagtatg cggccgagg tcagacgcc 
      aaaagaaga ggaaattgg aaaattagg gattatttt 
      gttgtattt tatatctaa taaagtatc tacacgagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY