Status
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Gene information
General information
TC008800 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10020688..10024017
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008800
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04545

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008800 RA
      MASTAELVT ASVADLQPK TFRTMTTKE RLHYIKNII 
      TVEPLIAAY LLASIICGP ALYNLEFEK GCRSNLQLN 
      DSICDAILS GEATNYSEE NEKIQILIG DMHSWQIPL 
      QSVMPLILV LFLGSYSDR HKLRKPFLL IPVLGEFFA 
      VAGCILCVV FMKEWPLAS QGIAQTVIP SFFGGQTMI 
      VMAVFAYIA DVSTVEMRT LRVGVVQIV LNVCTPVGQ 
      AVSGILFEK IGYYGVLSI AVVCYLFAI LYGIFWIKE 
      PHQPDVISK KSFFVDAFD PKHAVETFN LLFKKSKGN 
      NRAFVVAVL FILFIYSIV VTGEGGVFY LYVQGFFNW 
      GPLEYSYFI TINTVIHLV GTLIGVPLF TKFLSLGDL 
      MILLITFVD KIVTNIVFG LAKTDVMLY VATAVSIIT 
      GISNIAIRS LATKVVSQE DLGKAQSLF GICEAIGPA 
      IAAPLYNKA IYLNSLDTL PSAYFYFGA ILHVLLLVV 
      IIWMYYASK SEEKGDIEK EQPNNANEK VSEEFQMTH 
      I
    CDS sequence
    • >TC008800 RA
      atggcatcg acggcggaa ctggtcacc gcgtccgtc 
      gcggacctc cagccgaag accttccgg acgatgacc 
      accaaagag cgcctccac tacatcaag aacataatc 
      accgtggag cccctaatc gcggcgtac ctcctcgct 
      tccatcatc tgcgggccc gcgctgtac aacctcgag 
      ttcgagaag ggctgccgg tccaacctg cagctcaat 
      gactccatc tgcgacgcc atcctcagc ggagaggcc 
      acaaattac tcggaggag aacgagaaa atccagatt 
      ttgatcggg gacatgcac tcgtggcag atacccctc 
      cagagcgtc atgccccta attttggtc ctgtttttg 
      gggtcgtac agcgacagg cacaagctg cgcaagccc 
      tttctcttg attcccgtc ctgggggag tttttcgcc 
      gtcgcgggg tgcatcctg tgtgttgtt ttcatgaaa 
      gagtggccg ctggcgtca caagggatc gctcagacg 
      gtcatcccg tctttcttc gggggccag acgatgatt 
      gtcatggct gtttttgcc tacattgct gatgtcagc 
      actgtggag atgcggact ttaagggtc ggggttgtg 
      caaatcgtt ttgaacgtt tgcactcct gtgggacag 
      gctgttagt gggattttg tttgagaaa atcggctat 
      tatggagtt ttgtcgatt gccgttgtt tgttattta 
      ttcgcgatt ttgtacggg attttttgg attaaagaa 
      ccgcatcag cccgatgtt atttcaaag aaatcgttt 
      tttgtcgat gcttttgac cccaaacat gcggtggaa 
      actttcaat cttctattc aaaaagagc aaagggaat 
      aatcgagcg ttcgtggtc gccgttttg ttcattttg 
      ttcatttac agtatagtg gttacaggt gaaggagga 
      gttttttat ttgtacgtt caaggattt tttaactgg 
      ggtcctctt gaatacagt tactttatt actattaat 
      accgttatt catttagtg gggacatta attggggtt 
      cccctattt accaaattt ctctcactg ggggatttg 
      atgatttta ctcatcaca tttgtggat aaaattgta 
      acaaatatt gttttcggt ttggccaaa actgatgtc 
      atgttatat gttgccact gctgtaagc ataattacc 
      ggaatttct aacattgct ataagatca ttggctacg 
      aaagtggtt tcacaagag gatttagga aaagctcag 
      tctttattt ggaatttgt gaagccata ggtccagca 
      attgccgct ccgctgtac aataaagca atttattta 
      aacagttta gatacttta ccttcggct tatttttat 
      tttggagca attttacat gttttgttg ttggtggtg 
      ataatatgg atgtattat gcaagtaag tcggaagaa 
      aaaggtgac atcgaaaaa gaacagcca aacaatgca 
      aatgaaaaa gtctcagag gaattccag atgacccat 
      atttag
    mRNA sequence
    • >TC008800 RA
      ggcattacg aaaatttct ttactgttt tcgacatag 
      ttcaaaagt catcaccag agggcgtgt agttaattt 
      tatttccga attgaaatt gcttataag cagaatttg 
      aggcaaata agtatcaaa acaaataca atagcaaat 
      acgaagcct aagcaatgt ttttcttga taaattgaa 
      ccttcagca gtttttata atagttcaa atgctccgt 
      ggcaataat cacatggca aagaatgct accgtaaac 
      gggctttga aaatttgta actatactc acacacatg 
      agttcaata accaaattt ggtagatta tttaattat 
      agatattgt tatctccgt gtttttgta aatttcaat 
      acaatgacg tgacattgc ttattaata tttatttat 
      tccgcaacc ttaacatta agcgcatct tcatgaaaa 
      caagatccg tcatgctat ctcgcacta tcaaacgta 
      aaacgccag taaagttca aatgatcaa gataattag 
      ccactttaa atccccaac cggaatgca gcttgatca 
      tgtaacttt tatttcttt tttgccgtt tctcctcca 
      acatcttta aatcactgt aaggccacc gactcgccc 
      caagaaacc tgttctcag tgagaatcc atctattca 
      gctatgctg cacgcgatt tactatgaa accaaaaac 
      tgtccttaa gtgaagtgt ctttgacaa aaatggcat 
      cgacggcgg aactggtca ccgcgtccg tcgcggacc 
      tccagccga agaccttcc ggacgatga ccaccaaag 
      agcgcctcc actacatca agaacataa tcaccgtgg 
      agcccctaa tcgcggcgt acctcctcg cttccatca 
      tctgcgggc ccgcgctgt acaacctcg agttcgaga 
      agggctgcc ggtccaacc tgcagctca atgactcca 
      tctgcgacg ccatcctca gcggagagg ccacaaatt 
      actcggagg agaacgaga aaatccaga ttttgatcg 
      gggacatgc actcgtggc agatacccc tccagagcg 
      tcatgcccc taattttgg tcctgtttt tggggtcgt 
      acagcgaca ggcacaagc tgcgcaagc cctttctct 
      tgattcccg tcctggggg agtttttcg ccgtcgcgg 
      ggtgcatcc tgtgtgttg ttttcatga aagagtggc 
      cgctggcgt cacaaggga tcgctcaga cggtcatcc 
      cgtctttct tcgggggcc agacgatga ttgtcatgg 
      ctgtttttg cctacattg ctgatgtca gcactgtgg 
      agatgcgga ctttaaggg tcggggttg tgcaaatcg 
      ttttgaacg tttgcactc ctgtgggac aggctgtta 
      gtgggattt tgtttgaga aaatcggct attatggag 
      ttttgtcga ttgccgttg tttgttatt tattcgcga 
      ttttgtacg ggatttttt ggattaaag aaccgcatc 
      agcccgatg ttatttcaa agaaatcgt tttttgtcg 
      atgcttttg accccaaac atgcggtgg aaactttca 
      atcttctat tcaaaaaga gcaaaggga ataatcgag 
      cgttcgtgg tcgccgttt tgttcattt tgttcattt 
      acagtatag tggttacag gtgaaggag gagtttttt 
      atttgtacg ttcaaggat tttttaact ggggtcctc 
      ttgaataca gttacttta ttactatta ataccgtta 
      ttcatttag tggggacat taattgggg ttcccctat 
      ttaccaaat ttctctcac tgggggatt tgatgattt 
      tactcatca catttgtgg ataaaattg taacaaata 
      ttgttttcg gtttggcca aaactgatg tcatgttat 
      atgttgcca ctgctgtaa gcataatta ccggaattt 
      ctaacattg ctataagat cattggcta cgaaagtgg 
      tttcacaag aggatttag gaaaagctc agtctttat 
      ttggaattt gtgaagcca taggtccag caattgccg 
      ctccgctgt acaataaag caatttatt taaacagtt 
      tagatactt taccttcgg cttattttt attttggag 
      caattttac atgttttgt tgttggtgg tgataatat 
      ggatgtatt atgcaagta agtcggaag aaaaaggtg 
      acatcgaaa aagaacagc caaacaatg caaatgaaa 
      aagtctcag aggaattcc agatgaccc atatttagc 
      agaaacttg caatttgta aataataat gaaagagaa 
      actatttca ttttatata ttttttatg atgtattta 
      aaataaaaa ataaagttc ccagttttt a
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