Status
Please wait ...
Gene information
General information
TC033807
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008793 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9959698..9962619
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033807
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection

    Adult morphology
    elytra blistered - Number of animals showing the phenotype: 7


    elytra surface irregular - Number of animals showing the phenotype: 7


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033807 RA
      MSQKYSEDT GYVNIHGQD VKVLVCDKT GRILIPIDE 
VREMGFRIY RKNQYSRLL IDDKQFDRE FDLFMLQAA 
QTYMLEKKI AFNEHTVID SIQKCKKIL TEETSPNKF 
TRDHVIRHD SGDNITVTA NLRSPTLST MQVHNDQQN 
FSYKKRSPA SSVHSFDGR HNSPDYKRN NNFTHNQER 
SSSYDKKDQ NLNSSFELR KPKTVINAD GSPQFINGQ 
DDKKRRLNM QNQVKLSHV GLNNPEHTI LVTHVETSA 
PFFWAQDTE NSETLLEID AKVKEEAEK NKQICDFLL 
NKVYLAQHE GLWARCKVI KFKPLKVFF IDYGNIGEV 
TEIKEASEE LAKYPCQCV KLKLEDSSM KPNDFETLR 
VKVLNKHEN GTYTVVIAE TDDAFFSKP QPLCQLQHG 
QRVMIISQR DGKLALRTK ECYDKLKQV EQALAEVTS 
KPLSSVKKD QLVLFSRNS NLQRAVVTN FQENKVELE 
LLDYYDKVK VDLSSISNI TESLAKEPI SYLVTPSVK 
GLSDKTAEI VQNLIETRA KSTVVLNGD EFDLQLGKD 
LLSDKLKPP PGESHQKRT MLKDMTRFK PKLGVGEYI 
LNTFRNKDK LTIGCNDAI ESHLDHIIK TELDDNQPY 
EPITGEICF GSYLDSWSR CVVNRTMNS RYEVEFIDF 
GNVEILKGD QLRKLSDDV KNTPILGIP CRLIGLPHL 
SNVDELLKQ IVVEGDSYE INIKQLSGD HYEVEVPAL 
IKNLRESV
    • >TC033807 RB
      MSQKYSEDT GYVNIHGQD VKVLVCDKT GRILIPIDE 
VREMGFRIY RKNQYSRLL IDDKQFDRE FDLFMLQAA 
QTYMLEKKI AFNEHTVID SIQKKPKTV INADGSPQF 
INGQDDKKR RLNMQNQVK LSHVGLNNP EHTILVTHV 
ETSAPFFWA QDTENSETL LEIDAKVKE EAEKNKQIC 
DFLLNKVYL AQHEGLWAR CKVIKFKPL KVFFIDYGN 
IGEVTEIKE ASEELAKYP CQCVKLKLE DSSMKPNDF 
ETLRVKVLN KHENGTYTV VIAETDDAF FSKPQPLCQ 
LQHGQRVMI ISQRDGKLA LRTKECYDK LKQVEQALA 
EVTSKPLSS VKKDQLVLF SRNSNLQRA VVTNFQENK 
VELELLDYY DKVKVDLSS ISNITESLA KEPISYLVT 
PSVKGLSDK TAEIVQNLI ETRAKSTVV LNGDEFDLQ 
LGKDLLSDK LKPPPGESH QKRTMLKDM TRFKPKLGV 
GEYILNTFR NKDKLTIGC NDAIESHLD HIIKTELDD 
NQPYEPITG EICFGSYLD SWSRCVVNR TMNSRYEVE 
FIDFGNVEI LKGDQLRKL SDDVKNTPI LGIPCRLIG 
LPHLSNVDE LLKQIVVEG DSYEINIKQ LSGDHYEVE 
VPALIKNLR ESV
    CDS sequence
    • >TC033807 RA
      atgtctcaa aaatacagt gaagacact gggtatgtt 
aatatccac ggccaggat gttaaggtt ctcgtttgc 
gacaagacg gggcgaatt ctcattccc attgatgaa 
gtcagggaa atgggattc aggatctat aggaagaat 
cagtacagt agacttctt atcgacgat aaacaattt 
gatcgcgaa tttgacttg tttatgttg caagcggcc 
caaacgtac atgttagag aagaagata gccttcaat 
gaacataca gtaattgat agcattcaa aagtgcaag 
aaaattttg actgaagaa acatcccca aataaattt 
actcgggat catgtaatt aggcacgat tctggtgat 
aacataaca gtcacagca aacttgcgc tcacctact 
ttaagcacc atgcaagtg cataatgat caacagaat 
ttttcgtac aagaaacgc agtcccgca tcttccgtt 
cattctttc gatgggagg cacaattct cccgactac 
aagcgaaat aataatttt actcacaat caagaaaga 
tctagctca tatgataaa aaggatcag aatctcaat 
tcatctttt gaactcaga aagccaaaa actgttatt 
aatgcagac ggatcacca cagtttatc aatgggcaa 
gacgataag aagagacgt ctcaatatg cagaatcag 
gtcaagctc tcgcatgtt gggctaaat aaccctgaa 
catactatc ttagtgacc catgttgaa acaagtgct 
cccttcttc tgggcccag gacacggaa aacagtgaa 
acgctactt gagattgac gctaaggtt aaggaggag 
gctgagaaa aataagcaa atttgcgat ttcctccta 
aataaagtg tacttagca caacatgaa ggattatgg 
gctagatgc aaagtgatc aaatttaag cctctgaaa 
gtttttttt attgactat ggtaatatt ggtgaagtt 
accgaaatt aaagaggcc tccgaagaa ttagccaaa 
tatccttgc cagtgcgtt aaactgaaa ctggaggac 
tctagcatg aagccaaat gattttgaa acccttaga 
gttaaagtt ttgaacaag catgagaat ggcacttac 
actgttgtt atcgctgaa acagacgat gcttttttc 
tcaaaaccg cagcctttg tgtcagctt caacatggt 
cagagggtt atgattatt tcccaaaga gacgggaaa 
ctggctcta aggacgaaa gagtgctat gataagttg 
aaacaagtg gaacaagct ctagcagaa gtgacttct 
aaacctctg agcagcgtt aagaaagat caacttgtt 
ctcttcagt aggaacagt aacttgcaa agagcagtt 
gttacaaat tttcaggag aataaagtc gaacttgag 
ttgctagat tattatgac aaagttaag gtcgactta 
tcttcaata agcaatatt accgaatct ttggccaag 
gagccaatt tcatatctt gtcactccg tctgttaaa 
ggactctca gataaaaca gcggaaatt gtccaaaat 
ctgattgaa actcgtgcc aagtctact gttgtttta 
aatggtgac gagtttgat ctccaactt ggaaaggat 
ttgctgtct gataaacta aaaccacca cctggagaa 
tctcaccaa aagcgaaca atgttgaaa gacatgact 
cgctttaag cctaaatta ggcgtaggc gaatacatc 
cttaacact tttaggaac aaggataaa ttaaccatt 
ggttgcaat gacgctatt gaatctcac ttggaccac 
attatcaaa acagagctg gacgataat caaccgtat 
gaacccatc acaggcgag atttgtttt ggttcatac 
ttggatagt tggtccaga tgcgtagtc aatcgcacg 
atgaattcg cgatatgag gtcgagttc atcgatttt 
ggcaacgta gaaattctc aaaggggat cagctgaga 
aaattgtct gatgatgtt aagaatact cctatttta 
ggcattccg tgtcgctta attggccta ccgcattta 
tcaaacgtg gatgagtta ttgaagcag attgttgtt 
gaaggtgat agttacgaa ataaatatc aaacaactg 
agtggtgac cactatgaa gttgaagtt cctgctctc 
atcaagaat ctgagagag agtgtttaa
    • >TC033807 RB
      atgtctcaa aaatacagt gaagacact gggtatgtt 
aatatccac ggccaggat gttaaggtt ctcgtttgc 
gacaagacg gggcgaatt ctcattccc attgatgaa 
gtcagggaa atgggattc aggatctat aggaagaat 
cagtacagt agacttctt atcgacgat aaacaattt 
gatcgcgaa tttgacttg tttatgttg caagcggcc 
caaacgtac atgttagag aagaagata gccttcaat 
gaacataca gtaattgat agcattcaa aaaaagcca 
aaaactgtt attaatgca gacggatca ccacagttt 
atcaatggg caagacgat aagaagaga cgtctcaat 
atgcagaat caggtcaag ctctcgcat gttgggcta 
aataaccct gaacatact atcttagtg acccatgtt 
gaaacaagt gctcccttc ttctgggcc caggacacg 
gaaaacagt gaaacgcta cttgagatt gacgctaag 
gttaaggag gaggctgag aaaaataag caaatttgc 
gatttcctc ctaaataaa gtgtactta gcacaacat 
gaaggatta tgggctaga tgcaaagtg atcaaattt 
aagcctctg aaagttttt tttattgac tatggtaat 
attggtgaa gttaccgaa attaaagag gcctccgaa 
gaattagcc aaatatcct tgccagtgc gttaaactg 
aaactggag gactctagc atgaagcca aatgatttt 
gaaaccctt agagttaaa gttttgaac aagcatgag 
aatggcact tacactgtt gttatcgct gaaacagac 
gatgctttt ttctcaaaa ccgcagcct ttgtgtcag 
cttcaacat ggtcagagg gttatgatt atttcccaa 
agagacggg aaactggct ctaaggacg aaagagtgc 
tatgataag ttgaaacaa gtggaacaa gctctagca 
gaagtgact tctaaacct ctgagcagc gttaagaaa 
gatcaactt gttctcttc agtaggaac agtaacttg 
caaagagca gttgttaca aattttcag gagaataaa 
gtcgaactt gagttgcta gattattat gacaaagtt 
aaggtcgac ttatcttca ataagcaat attaccgaa 
tctttggcc aaggagcca atttcatat cttgtcact 
ccgtctgtt aaaggactc tcagataaa acagcggaa 
attgtccaa aatctgatt gaaactcgt gccaagtct 
actgttgtt ttaaatggt gacgagttt gatctccaa 
cttggaaag gatttgctg tctgataaa ctaaaacca 
ccacctgga gaatctcac caaaagcga acaatgttg 
aaagacatg actcgcttt aagcctaaa ttaggcgta 
ggcgaatac atccttaac acttttagg aacaaggat 
aaattaacc attggttgc aatgacgct attgaatct 
cacttggac cacattatc aaaacagag ctggacgat 
aatcaaccg tatgaaccc atcacaggc gagatttgt 
tttggttca tacttggat agttggtcc agatgcgta 
gtcaatcgc acgatgaat tcgcgatat gaggtcgag 
ttcatcgat tttggcaac gtagaaatt ctcaaaggg 
gatcagctg agaaaattg tctgatgat gttaagaat 
actcctatt ttaggcatt ccgtgtcgc ttaattggc 
ctaccgcat ttatcaaac gtggatgag ttattgaag 
cagattgtt gttgaaggt gatagttac gaaataaat 
atcaaacaa ctgagtggt gaccactat gaagttgaa 
gttcctgct ctcatcaag aatctgaga gagagtgtt 
taa
    mRNA sequence
    • >TC033807 RA
      gaacatttg tagaccggc aagggctag ccgctagta 
acgtcttct ggttggtcg aattgattt aaacaactt 
aaggagggt gtaacgagg atggaactg gctttgtga 
gattctttt tccacgaaa atttagtca acgcgtgtt 
aagaaaatc ggttaagtt tttccatct ttaactgtg 
cttcattta attccaact atgtctcaa aaatacagt 
gaagacact gggtatgtt aatatccac ggccaggat 
gttaaggtt ctcgtttgc gacaagacg gggcgaatt 
ctcattccc attgatgaa gtcagggaa atgggattc 
aggatctat aggaagaat cagtacagt agacttctt 
atcgacgat aaacaattt gatcgcgaa tttgacttg 
tttatgttg caagcggcc caaacgtac atgttagag 
aagaagata gccttcaat gaacataca gtaattgat 
agcattcaa aagtgcaag aaaattttg actgaagaa 
acatcccca aataaattt actcgggat catgtaatt 
aggcacgat tctggtgat aacataaca gtcacagca 
aacttgcgc tcacctact ttaagcacc atgcaagtg 
cataatgat caacagaat ttttcgtac aagaaacgc 
agtcccgca tcttccgtt cattctttc gatgggagg 
cacaattct cccgactac aagcgaaat aataatttt 
actcacaat caagaaaga tctagctca tatgataaa 
aaggatcag aatctcaat tcatctttt gaactcaga 
aagccaaaa actgttatt aatgcagac ggatcacca 
cagtttatc aatgggcaa gacgataag aagagacgt 
ctcaatatg cagaatcag gtcaagctc tcgcatgtt 
gggctaaat aaccctgaa catactatc ttagtgacc 
catgttgaa acaagtgct cccttcttc tgggcccag 
gacacggaa aacagtgaa acgctactt gagattgac 
gctaaggtt aaggaggag gctgagaaa aataagcaa 
atttgcgat ttcctccta aataaagtg tacttagca 
caacatgaa ggattatgg gctagatgc aaagtgatc 
aaatttaag cctctgaaa gtttttttt attgactat 
ggtaatatt ggtgaagtt accgaaatt aaagaggcc 
tccgaagaa ttagccaaa tatccttgc cagtgcgtt 
aaactgaaa ctggaggac tctagcatg aagccaaat 
gattttgaa acccttaga gttaaagtt ttgaacaag 
catgagaat ggcacttac actgttgtt atcgctgaa 
acagacgat gcttttttc tcaaaaccg cagcctttg 
tgtcagctt caacatggt cagagggtt atgattatt 
tcccaaaga gacgggaaa ctggctcta aggacgaaa 
gagtgctat gataagttg aaacaagtg gaacaagct 
ctagcagaa gtgacttct aaacctctg agcagcgtt 
aagaaagat caacttgtt ctcttcagt aggaacagt 
aacttgcaa agagcagtt gttacaaat tttcaggag 
aataaagtc gaacttgag ttgctagat tattatgac 
aaagttaag gtcgactta tcttcaata agcaatatt 
accgaatct ttggccaag gagccaatt tcatatctt 
gtcactccg tctgttaaa ggactctca gataaaaca 
gcggaaatt gtccaaaat ctgattgaa actcgtgcc 
aagtctact gttgtttta aatggtgac gagtttgat 
ctccaactt ggaaaggat ttgctgtct gataaacta 
aaaccacca cctggagaa tctcaccaa aagcgaaca 
atgttgaaa gacatgact cgctttaag cctaaatta 
ggcgtaggc gaatacatc cttaacact tttaggaac 
aaggataaa ttaaccatt ggttgcaat gacgctatt 
gaatctcac ttggaccac attatcaaa acagagctg 
gacgataat caaccgtat gaacccatc acaggcgag 
atttgtttt ggttcatac ttggatagt tggtccaga 
tgcgtagtc aatcgcacg atgaattcg cgatatgag 
gtcgagttc atcgatttt ggcaacgta gaaattctc 
aaaggggat cagctgaga aaattgtct gatgatgtt 
aagaatact cctatttta ggcattccg tgtcgctta 
attggccta ccgcattta tcaaacgtg gatgagtta 
ttgaagcag attgttgtt gaaggtgat agttacgaa 
ataaatatc aaacaactg agtggtgac cactatgaa 
gttgaagtt cctgctctc atcaagaat ctgagagag 
agtgtttaa tttttatgt ttcttacat gttctaagt 
ttagttgat ttacaattt cgattgtgt tgttttgat 
caaactttt ttaagttac cttttaaga aataaattt 
tacttacat gc
    • >TC033807 RB
      gaacatttg tagaccggc aagggctag ccgctagta 
acgtcttct ggttggtcg aattgattt aaacaactt 
aaggagggt gtaacgagg atggaactg gctttgtga 
gattctttt tccacgaaa atttagtca acgcgtgtt 
aagaaaatc ggttaagtt tttccatct ttaactgtg 
cttcattta attccaact atgtctcaa aaatacagt 
gaagacact gggtatgtt aatatccac ggccaggat 
gttaaggtt ctcgtttgc gacaagacg gggcgaatt 
ctcattccc attgatgaa gtcagggaa atgggattc 
aggatctat aggaagaat cagtacagt agacttctt 
atcgacgat aaacaattt gatcgcgaa tttgacttg 
tttatgttg caagcggcc caaacgtac atgttagag 
aagaagata gccttcaat gaacataca gtaattgat 
agcattcaa aaaaagcca aaaactgtt attaatgca 
gacggatca ccacagttt atcaatggg caagacgat 
aagaagaga cgtctcaat atgcagaat caggtcaag 
ctctcgcat gttgggcta aataaccct gaacatact 
atcttagtg acccatgtt gaaacaagt gctcccttc 
ttctgggcc caggacacg gaaaacagt gaaacgcta 
cttgagatt gacgctaag gttaaggag gaggctgag 
aaaaataag caaatttgc gatttcctc ctaaataaa 
gtgtactta gcacaacat gaaggatta tgggctaga 
tgcaaagtg atcaaattt aagcctctg aaagttttt 
tttattgac tatggtaat attggtgaa gttaccgaa 
attaaagag gcctccgaa gaattagcc aaatatcct 
tgccagtgc gttaaactg aaactggag gactctagc 
atgaagcca aatgatttt gaaaccctt agagttaaa 
gttttgaac aagcatgag aatggcact tacactgtt 
gttatcgct gaaacagac gatgctttt ttctcaaaa 
ccgcagcct ttgtgtcag cttcaacat ggtcagagg 
gttatgatt atttcccaa agagacggg aaactggct 
ctaaggacg aaagagtgc tatgataag ttgaaacaa 
gtggaacaa gctctagca gaagtgact tctaaacct 
ctgagcagc gttaagaaa gatcaactt gttctcttc 
agtaggaac agtaacttg caaagagca gttgttaca 
aattttcag gagaataaa gtcgaactt gagttgcta 
gattattat gacaaagtt aaggtcgac ttatcttca 
ataagcaat attaccgaa tctttggcc aaggagcca 
atttcatat cttgtcact ccgtctgtt aaaggactc 
tcagataaa acagcggaa attgtccaa aatctgatt 
gaaactcgt gccaagtct actgttgtt ttaaatggt 
gacgagttt gatctccaa cttggaaag gatttgctg 
tctgataaa ctaaaacca ccacctgga gaatctcac 
caaaagcga acaatgttg aaagacatg actcgcttt 
aagcctaaa ttaggcgta ggcgaatac atccttaac 
acttttagg aacaaggat aaattaacc attggttgc 
aatgacgct attgaatct cacttggac cacattatc 
aaaacagag ctggacgat aatcaaccg tatgaaccc 
atcacaggc gagatttgt tttggttca tacttggat 
agttggtcc agatgcgta gtcaatcgc acgatgaat 
tcgcgatat gaggtcgag ttcatcgat tttggcaac 
gtagaaatt ctcaaaggg gatcagctg agaaaattg 
tctgatgat gttaagaat actcctatt ttaggcatt 
ccgtgtcgc ttaattggc ctaccgcat ttatcaaac 
gtggatgag ttattgaag cagattgtt gttgaaggt 
gatagttac gaaataaat atcaaacaa ctgagtggt 
gaccactat gaagttgaa gttcctgct ctcatcaag 
aatctgaga gagagtgtt taattttta tgtttctta 
catgttcta agtttagtt gatttacaa tttcgattg 
tgttgtttt gatcaaact tttttaagt tacctttta 
agaaataaa ttttactta catgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY