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Gene information
General information
TC033807
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008793
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9959698..9962619
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033807
    iBeetle screen iB_04543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    Adult morphology
    elytra blistered - Number of animals showing the phenotype: 7


    elytra surface irregular - Number of animals showing the phenotype: 7


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033807 RA
      MSQKYSEDT GYVNIHGQD VKVLVCDKT GRILIPIDE 
      VREMGFRIY RKNQYSRLL IDDKQFDRE FDLFMLQAA 
      QTYMLEKKI AFNEHTVID SIQKCKKIL TEETSPNKF 
      TRDHVIRHD SGDNITVTA NLRSPTLST MQVHNDQQN 
      FSYKKRSPA SSVHSFDGR HNSPDYKRN NNFTHNQER 
      SSSYDKKDQ NLNSSFELR KPKTVINAD GSPQFINGQ 
      DDKKRRLNM QNQVKLSHV GLNNPEHTI LVTHVETSA 
      PFFWAQDTE NSETLLEID AKVKEEAEK NKQICDFLL 
      NKVYLAQHE GLWARCKVI KFKPLKVFF IDYGNIGEV 
      TEIKEASEE LAKYPCQCV KLKLEDSSM KPNDFETLR 
      VKVLNKHEN GTYTVVIAE TDDAFFSKP QPLCQLQHG 
      QRVMIISQR DGKLALRTK ECYDKLKQV EQALAEVTS 
      KPLSSVKKD QLVLFSRNS NLQRAVVTN FQENKVELE 
      LLDYYDKVK VDLSSISNI TESLAKEPI SYLVTPSVK 
      GLSDKTAEI VQNLIETRA KSTVVLNGD EFDLQLGKD 
      LLSDKLKPP PGESHQKRT MLKDMTRFK PKLGVGEYI 
      LNTFRNKDK LTIGCNDAI ESHLDHIIK TELDDNQPY 
      EPITGEICF GSYLDSWSR CVVNRTMNS RYEVEFIDF 
      GNVEILKGD QLRKLSDDV KNTPILGIP CRLIGLPHL 
      SNVDELLKQ IVVEGDSYE INIKQLSGD HYEVEVPAL 
      IKNLRESV
    • >TC033807 RB
      MSQKYSEDT GYVNIHGQD VKVLVCDKT GRILIPIDE 
      VREMGFRIY RKNQYSRLL IDDKQFDRE FDLFMLQAA 
      QTYMLEKKI AFNEHTVID SIQKKPKTV INADGSPQF 
      INGQDDKKR RLNMQNQVK LSHVGLNNP EHTILVTHV 
      ETSAPFFWA QDTENSETL LEIDAKVKE EAEKNKQIC 
      DFLLNKVYL AQHEGLWAR CKVIKFKPL KVFFIDYGN 
      IGEVTEIKE ASEELAKYP CQCVKLKLE DSSMKPNDF 
      ETLRVKVLN KHENGTYTV VIAETDDAF FSKPQPLCQ 
      LQHGQRVMI ISQRDGKLA LRTKECYDK LKQVEQALA 
      EVTSKPLSS VKKDQLVLF SRNSNLQRA VVTNFQENK 
      VELELLDYY DKVKVDLSS ISNITESLA KEPISYLVT 
      PSVKGLSDK TAEIVQNLI ETRAKSTVV LNGDEFDLQ 
      LGKDLLSDK LKPPPGESH QKRTMLKDM TRFKPKLGV 
      GEYILNTFR NKDKLTIGC NDAIESHLD HIIKTELDD 
      NQPYEPITG EICFGSYLD SWSRCVVNR TMNSRYEVE 
      FIDFGNVEI LKGDQLRKL SDDVKNTPI LGIPCRLIG 
      LPHLSNVDE LLKQIVVEG DSYEINIKQ LSGDHYEVE 
      VPALIKNLR ESV
    CDS sequence
    • >TC033807 RA
      atgtctcaa aaatacagt gaagacact gggtatgtt 
      aatatccac ggccaggat gttaaggtt ctcgtttgc 
      gacaagacg gggcgaatt ctcattccc attgatgaa 
      gtcagggaa atgggattc aggatctat aggaagaat 
      cagtacagt agacttctt atcgacgat aaacaattt 
      gatcgcgaa tttgacttg tttatgttg caagcggcc 
      caaacgtac atgttagag aagaagata gccttcaat 
      gaacataca gtaattgat agcattcaa aagtgcaag 
      aaaattttg actgaagaa acatcccca aataaattt 
      actcgggat catgtaatt aggcacgat tctggtgat 
      aacataaca gtcacagca aacttgcgc tcacctact 
      ttaagcacc atgcaagtg cataatgat caacagaat 
      ttttcgtac aagaaacgc agtcccgca tcttccgtt 
      cattctttc gatgggagg cacaattct cccgactac 
      aagcgaaat aataatttt actcacaat caagaaaga 
      tctagctca tatgataaa aaggatcag aatctcaat 
      tcatctttt gaactcaga aagccaaaa actgttatt 
      aatgcagac ggatcacca cagtttatc aatgggcaa 
      gacgataag aagagacgt ctcaatatg cagaatcag 
      gtcaagctc tcgcatgtt gggctaaat aaccctgaa 
      catactatc ttagtgacc catgttgaa acaagtgct 
      cccttcttc tgggcccag gacacggaa aacagtgaa 
      acgctactt gagattgac gctaaggtt aaggaggag 
      gctgagaaa aataagcaa atttgcgat ttcctccta 
      aataaagtg tacttagca caacatgaa ggattatgg 
      gctagatgc aaagtgatc aaatttaag cctctgaaa 
      gtttttttt attgactat ggtaatatt ggtgaagtt 
      accgaaatt aaagaggcc tccgaagaa ttagccaaa 
      tatccttgc cagtgcgtt aaactgaaa ctggaggac 
      tctagcatg aagccaaat gattttgaa acccttaga 
      gttaaagtt ttgaacaag catgagaat ggcacttac 
      actgttgtt atcgctgaa acagacgat gcttttttc 
      tcaaaaccg cagcctttg tgtcagctt caacatggt 
      cagagggtt atgattatt tcccaaaga gacgggaaa 
      ctggctcta aggacgaaa gagtgctat gataagttg 
      aaacaagtg gaacaagct ctagcagaa gtgacttct 
      aaacctctg agcagcgtt aagaaagat caacttgtt 
      ctcttcagt aggaacagt aacttgcaa agagcagtt 
      gttacaaat tttcaggag aataaagtc gaacttgag 
      ttgctagat tattatgac aaagttaag gtcgactta 
      tcttcaata agcaatatt accgaatct ttggccaag 
      gagccaatt tcatatctt gtcactccg tctgttaaa 
      ggactctca gataaaaca gcggaaatt gtccaaaat 
      ctgattgaa actcgtgcc aagtctact gttgtttta 
      aatggtgac gagtttgat ctccaactt ggaaaggat 
      ttgctgtct gataaacta aaaccacca cctggagaa 
      tctcaccaa aagcgaaca atgttgaaa gacatgact 
      cgctttaag cctaaatta ggcgtaggc gaatacatc 
      cttaacact tttaggaac aaggataaa ttaaccatt 
      ggttgcaat gacgctatt gaatctcac ttggaccac 
      attatcaaa acagagctg gacgataat caaccgtat 
      gaacccatc acaggcgag atttgtttt ggttcatac 
      ttggatagt tggtccaga tgcgtagtc aatcgcacg 
      atgaattcg cgatatgag gtcgagttc atcgatttt 
      ggcaacgta gaaattctc aaaggggat cagctgaga 
      aaattgtct gatgatgtt aagaatact cctatttta 
      ggcattccg tgtcgctta attggccta ccgcattta 
      tcaaacgtg gatgagtta ttgaagcag attgttgtt 
      gaaggtgat agttacgaa ataaatatc aaacaactg 
      agtggtgac cactatgaa gttgaagtt cctgctctc 
      atcaagaat ctgagagag agtgtttaa
    • >TC033807 RB
      atgtctcaa aaatacagt gaagacact gggtatgtt 
      aatatccac ggccaggat gttaaggtt ctcgtttgc 
      gacaagacg gggcgaatt ctcattccc attgatgaa 
      gtcagggaa atgggattc aggatctat aggaagaat 
      cagtacagt agacttctt atcgacgat aaacaattt 
      gatcgcgaa tttgacttg tttatgttg caagcggcc 
      caaacgtac atgttagag aagaagata gccttcaat 
      gaacataca gtaattgat agcattcaa aaaaagcca 
      aaaactgtt attaatgca gacggatca ccacagttt 
      atcaatggg caagacgat aagaagaga cgtctcaat 
      atgcagaat caggtcaag ctctcgcat gttgggcta 
      aataaccct gaacatact atcttagtg acccatgtt 
      gaaacaagt gctcccttc ttctgggcc caggacacg 
      gaaaacagt gaaacgcta cttgagatt gacgctaag 
      gttaaggag gaggctgag aaaaataag caaatttgc 
      gatttcctc ctaaataaa gtgtactta gcacaacat 
      gaaggatta tgggctaga tgcaaagtg atcaaattt 
      aagcctctg aaagttttt tttattgac tatggtaat 
      attggtgaa gttaccgaa attaaagag gcctccgaa 
      gaattagcc aaatatcct tgccagtgc gttaaactg 
      aaactggag gactctagc atgaagcca aatgatttt 
      gaaaccctt agagttaaa gttttgaac aagcatgag 
      aatggcact tacactgtt gttatcgct gaaacagac 
      gatgctttt ttctcaaaa ccgcagcct ttgtgtcag 
      cttcaacat ggtcagagg gttatgatt atttcccaa 
      agagacggg aaactggct ctaaggacg aaagagtgc 
      tatgataag ttgaaacaa gtggaacaa gctctagca 
      gaagtgact tctaaacct ctgagcagc gttaagaaa 
      gatcaactt gttctcttc agtaggaac agtaacttg 
      caaagagca gttgttaca aattttcag gagaataaa 
      gtcgaactt gagttgcta gattattat gacaaagtt 
      aaggtcgac ttatcttca ataagcaat attaccgaa 
      tctttggcc aaggagcca atttcatat cttgtcact 
      ccgtctgtt aaaggactc tcagataaa acagcggaa 
      attgtccaa aatctgatt gaaactcgt gccaagtct 
      actgttgtt ttaaatggt gacgagttt gatctccaa 
      cttggaaag gatttgctg tctgataaa ctaaaacca 
      ccacctgga gaatctcac caaaagcga acaatgttg 
      aaagacatg actcgcttt aagcctaaa ttaggcgta 
      ggcgaatac atccttaac acttttagg aacaaggat 
      aaattaacc attggttgc aatgacgct attgaatct 
      cacttggac cacattatc aaaacagag ctggacgat 
      aatcaaccg tatgaaccc atcacaggc gagatttgt 
      tttggttca tacttggat agttggtcc agatgcgta 
      gtcaatcgc acgatgaat tcgcgatat gaggtcgag 
      ttcatcgat tttggcaac gtagaaatt ctcaaaggg 
      gatcagctg agaaaattg tctgatgat gttaagaat 
      actcctatt ttaggcatt ccgtgtcgc ttaattggc 
      ctaccgcat ttatcaaac gtggatgag ttattgaag 
      cagattgtt gttgaaggt gatagttac gaaataaat 
      atcaaacaa ctgagtggt gaccactat gaagttgaa 
      gttcctgct ctcatcaag aatctgaga gagagtgtt 
      taa
    mRNA sequence
    • >TC033807 RA
      gaacatttg tagaccggc aagggctag ccgctagta 
      acgtcttct ggttggtcg aattgattt aaacaactt 
      aaggagggt gtaacgagg atggaactg gctttgtga 
      gattctttt tccacgaaa atttagtca acgcgtgtt 
      aagaaaatc ggttaagtt tttccatct ttaactgtg 
      cttcattta attccaact atgtctcaa aaatacagt 
      gaagacact gggtatgtt aatatccac ggccaggat 
      gttaaggtt ctcgtttgc gacaagacg gggcgaatt 
      ctcattccc attgatgaa gtcagggaa atgggattc 
      aggatctat aggaagaat cagtacagt agacttctt 
      atcgacgat aaacaattt gatcgcgaa tttgacttg 
      tttatgttg caagcggcc caaacgtac atgttagag 
      aagaagata gccttcaat gaacataca gtaattgat 
      agcattcaa aagtgcaag aaaattttg actgaagaa 
      acatcccca aataaattt actcgggat catgtaatt 
      aggcacgat tctggtgat aacataaca gtcacagca 
      aacttgcgc tcacctact ttaagcacc atgcaagtg 
      cataatgat caacagaat ttttcgtac aagaaacgc 
      agtcccgca tcttccgtt cattctttc gatgggagg 
      cacaattct cccgactac aagcgaaat aataatttt 
      actcacaat caagaaaga tctagctca tatgataaa 
      aaggatcag aatctcaat tcatctttt gaactcaga 
      aagccaaaa actgttatt aatgcagac ggatcacca 
      cagtttatc aatgggcaa gacgataag aagagacgt 
      ctcaatatg cagaatcag gtcaagctc tcgcatgtt 
      gggctaaat aaccctgaa catactatc ttagtgacc 
      catgttgaa acaagtgct cccttcttc tgggcccag 
      gacacggaa aacagtgaa acgctactt gagattgac 
      gctaaggtt aaggaggag gctgagaaa aataagcaa 
      atttgcgat ttcctccta aataaagtg tacttagca 
      caacatgaa ggattatgg gctagatgc aaagtgatc 
      aaatttaag cctctgaaa gtttttttt attgactat 
      ggtaatatt ggtgaagtt accgaaatt aaagaggcc 
      tccgaagaa ttagccaaa tatccttgc cagtgcgtt 
      aaactgaaa ctggaggac tctagcatg aagccaaat 
      gattttgaa acccttaga gttaaagtt ttgaacaag 
      catgagaat ggcacttac actgttgtt atcgctgaa 
      acagacgat gcttttttc tcaaaaccg cagcctttg 
      tgtcagctt caacatggt cagagggtt atgattatt 
      tcccaaaga gacgggaaa ctggctcta aggacgaaa 
      gagtgctat gataagttg aaacaagtg gaacaagct 
      ctagcagaa gtgacttct aaacctctg agcagcgtt 
      aagaaagat caacttgtt ctcttcagt aggaacagt 
      aacttgcaa agagcagtt gttacaaat tttcaggag 
      aataaagtc gaacttgag ttgctagat tattatgac 
      aaagttaag gtcgactta tcttcaata agcaatatt 
      accgaatct ttggccaag gagccaatt tcatatctt 
      gtcactccg tctgttaaa ggactctca gataaaaca 
      gcggaaatt gtccaaaat ctgattgaa actcgtgcc 
      aagtctact gttgtttta aatggtgac gagtttgat 
      ctccaactt ggaaaggat ttgctgtct gataaacta 
      aaaccacca cctggagaa tctcaccaa aagcgaaca 
      atgttgaaa gacatgact cgctttaag cctaaatta 
      ggcgtaggc gaatacatc cttaacact tttaggaac 
      aaggataaa ttaaccatt ggttgcaat gacgctatt 
      gaatctcac ttggaccac attatcaaa acagagctg 
      gacgataat caaccgtat gaacccatc acaggcgag 
      atttgtttt ggttcatac ttggatagt tggtccaga 
      tgcgtagtc aatcgcacg atgaattcg cgatatgag 
      gtcgagttc atcgatttt ggcaacgta gaaattctc 
      aaaggggat cagctgaga aaattgtct gatgatgtt 
      aagaatact cctatttta ggcattccg tgtcgctta 
      attggccta ccgcattta tcaaacgtg gatgagtta 
      ttgaagcag attgttgtt gaaggtgat agttacgaa 
      ataaatatc aaacaactg agtggtgac cactatgaa 
      gttgaagtt cctgctctc atcaagaat ctgagagag 
      agtgtttaa tttttatgt ttcttacat gttctaagt 
      ttagttgat ttacaattt cgattgtgt tgttttgat 
      caaactttt ttaagttac cttttaaga aataaattt 
      tacttacat gc
    • >TC033807 RB
      gaacatttg tagaccggc aagggctag ccgctagta 
      acgtcttct ggttggtcg aattgattt aaacaactt 
      aaggagggt gtaacgagg atggaactg gctttgtga 
      gattctttt tccacgaaa atttagtca acgcgtgtt 
      aagaaaatc ggttaagtt tttccatct ttaactgtg 
      cttcattta attccaact atgtctcaa aaatacagt 
      gaagacact gggtatgtt aatatccac ggccaggat 
      gttaaggtt ctcgtttgc gacaagacg gggcgaatt 
      ctcattccc attgatgaa gtcagggaa atgggattc 
      aggatctat aggaagaat cagtacagt agacttctt 
      atcgacgat aaacaattt gatcgcgaa tttgacttg 
      tttatgttg caagcggcc caaacgtac atgttagag 
      aagaagata gccttcaat gaacataca gtaattgat 
      agcattcaa aaaaagcca aaaactgtt attaatgca 
      gacggatca ccacagttt atcaatggg caagacgat 
      aagaagaga cgtctcaat atgcagaat caggtcaag 
      ctctcgcat gttgggcta aataaccct gaacatact 
      atcttagtg acccatgtt gaaacaagt gctcccttc 
      ttctgggcc caggacacg gaaaacagt gaaacgcta 
      cttgagatt gacgctaag gttaaggag gaggctgag 
      aaaaataag caaatttgc gatttcctc ctaaataaa 
      gtgtactta gcacaacat gaaggatta tgggctaga 
      tgcaaagtg atcaaattt aagcctctg aaagttttt 
      tttattgac tatggtaat attggtgaa gttaccgaa 
      attaaagag gcctccgaa gaattagcc aaatatcct 
      tgccagtgc gttaaactg aaactggag gactctagc 
      atgaagcca aatgatttt gaaaccctt agagttaaa 
      gttttgaac aagcatgag aatggcact tacactgtt 
      gttatcgct gaaacagac gatgctttt ttctcaaaa 
      ccgcagcct ttgtgtcag cttcaacat ggtcagagg 
      gttatgatt atttcccaa agagacggg aaactggct 
      ctaaggacg aaagagtgc tatgataag ttgaaacaa 
      gtggaacaa gctctagca gaagtgact tctaaacct 
      ctgagcagc gttaagaaa gatcaactt gttctcttc 
      agtaggaac agtaacttg caaagagca gttgttaca 
      aattttcag gagaataaa gtcgaactt gagttgcta 
      gattattat gacaaagtt aaggtcgac ttatcttca 
      ataagcaat attaccgaa tctttggcc aaggagcca 
      atttcatat cttgtcact ccgtctgtt aaaggactc 
      tcagataaa acagcggaa attgtccaa aatctgatt 
      gaaactcgt gccaagtct actgttgtt ttaaatggt 
      gacgagttt gatctccaa cttggaaag gatttgctg 
      tctgataaa ctaaaacca ccacctgga gaatctcac 
      caaaagcga acaatgttg aaagacatg actcgcttt 
      aagcctaaa ttaggcgta ggcgaatac atccttaac 
      acttttagg aacaaggat aaattaacc attggttgc 
      aatgacgct attgaatct cacttggac cacattatc 
      aaaacagag ctggacgat aatcaaccg tatgaaccc 
      atcacaggc gagatttgt tttggttca tacttggat 
      agttggtcc agatgcgta gtcaatcgc acgatgaat 
      tcgcgatat gaggtcgag ttcatcgat tttggcaac 
      gtagaaatt ctcaaaggg gatcagctg agaaaattg 
      tctgatgat gttaagaat actcctatt ttaggcatt 
      ccgtgtcgc ttaattggc ctaccgcat ttatcaaac 
      gtggatgag ttattgaag cagattgtt gttgaaggt 
      gatagttac gaaataaat atcaaacaa ctgagtggt 
      gaccactat gaagttgaa gttcctgct ctcatcaag 
      aatctgaga gagagtgtt taattttta tgtttctta 
      catgttcta agtttagtt gatttacaa tttcgattg 
      tgttgtttt gatcaaact tttttaagt tacctttta 
      agaaataaa ttttactta catgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY