Status
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Gene information
General information
TC033821
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008774,TC008775
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9749683..9798999
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033821
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04541

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033821 RA
      MSSPGDAAE PAPPPPDPQ VVRREPPPS ERPPRPVSY 
      PRGPESSPR TGGFVKRNS DPAPAAALR PRTQVKPFT 
      KESLERLER KTVQLVREY GFQPRRKLS VEDGSRLPA 
      KFEPFPAKL YGRPLEEID NFIYDETFC VVSKRFRKN 
      YIHRFTATN SFFFFSPFS SVRKACIYL STNQYFDYL 
      VMATILLNC VFLAMAETI EEAEYIFLA IYTAEMIIK 
      SIAKGFILN KYTYLRNPW NWLDFVVIT SGYATIGME 
      VGNLAGLRT FRVLRALKT ISILPVLFT CTMQRVLTL 
      TVVLFIKIT VSSRCDSFQ NVTIKVHPD RGNPYDTTI 
      TGCIERIAF ENVITEAHI KHQKIPKLG RDSVRNMKH 
      LHTITFDNC EIKSIDPVA FRNVPNLKT IEILNGNLT 
      YVSAGVFLL APKLETLSL SHNKLRQIE DGTFSGVSN 
      LKRLSCESN NLGIWRPQW FTNSTNLQI INFQFNKIK 
      ILPRRAFFE LRKLEAIYF NYNELEVIQ PEAFKGITH 
      LKYLGLRYN RLKTIDPRS FPNVIMIDS LTIAANYLN 
      AVSTKLWIS VKTKNVNLD GNPWKCLCL DQVQLQLHE 
      MNATVQTSD GCVSGDVPV CSFPAVHST TCNETVDDD 
      LTNTYFAEL RKVNTPLDK YCARLD
    • >TC033821 RB
      MPPHRSDAV VFGGGAVAS RRARFDSPS NSMSSPGDA 
      AEPAPPPPD PQVVRREPP PSERPPRPV SYPRGPESS 
      PRTGGFVKR NSDPAPAAA LRPRTQVKP FTKESLERL 
      ERKTVQLVR EYGFQPRRK LSVEDGSRL PAKFEPFPA 
      KLYGRPLEE IDNFIYDET FCVVSKRFR KNYIHRFTA 
      TNSFFFFSP FSSVRKACI YLSTNQYFD YLVMATILL 
      NCVFLAMAE TIEEAEYIF LAIYTAEMI IKSIAKGFI 
      LNKYTYLRN PWNWLDFVV ITSGYATIG MEVGNLAGL 
      RTFRVLRAL KTISILPVL FTCTMQRVL TLTVVLFIK 
      ITVSSRCDS FQNVTIKVH PDRGNPYDT TITGCIERI 
      AFENVITEA HIKHQKIPK LGRDSVRNM KHLHTITFD 
      NCEIKSIDP VAFRNVPNL KTIEILNGN LTYVSAGVF 
      LLAPKLETL SLSHNKLRQ IEDGTFSGV SNLKRLSCE 
      SNNLGIWRP QWFTNSTNL QIINFQFNK IKILPRRAF 
      FELRKLEAI YFNYNELEV IQPEAFKGI THLKYLGLR 
      YNRLKTIDP RSFPNVIMI DSLTIAANY LNAVSTKLW 
      ISVKTKNVN LDGNPWKCL CLDQVQLQL HEMNATVQT 
      SDGCVSGDV PVCSFPAVH STTCNETVD DDLTNTYFA 
      ELRKVNTPL DKYCARLD
    CDS sequence
    • >TC033821 RA
      atgtcgagt ccaggcgac gcggccgag ccggctcca 
      ccgccgccg gacccccag gtggtgcgc cgcgaaccg 
      ccgccgtcc gagcgcccg ccgcggccg gtgtcgtac 
      cctcgcggg ccggagagc tccccccgc accggcggc 
      ttcgtcaaa cgcaactcg gaccccgcg cccgcggcc 
      gccctcagg ccccgcacc caggtcaag cccttcacc 
      aaggagtcg ctcgagcgg ctcgagagg aaaactgtg 
      caactggtg cgcgagtac ggcttccag ccgaggagg 
      aaactcagt gttgaggat ggctcgcgg ctgcccgcc 
      aagttcgag cccttcccc gccaaactc tacggcagg 
      ccgcttgag gaaatagac aatttcatt tatgacgag 
      accttctgc gtggtttcg aagaggttt cgcaagaac 
      tacatccac cgtttcacc gccaccaat tcgttcttc 
      ttcttcagt ccgtttagt tcagttagg aaagcgtgt 
      atatacctg tccaccaac cagtacttc gattattta 
      gttatggct actattttg ctcaactgc gtcttcctt 
      gccatggcg gaaactatt gaagaagcc gagtatatt 
      tttttagcc atatatacg gctgaaatg ataataaag 
      agcatcgct aaaggtttt atattaaat aagtacaca 
      tatctaagg aatccatgg aattggttg gacttcgtc 
      gtcatcacc agcggctac gccaccatc ggcatggag 
      gtggggaac ctcgccggt ctgcgaacg tttcgcgtc 
      ttacgagcc ctcaagacc atctcgatt ctcccagta 
      cttttcacg tgcaccatg cagcgagtc ttgacctta 
      actgtggtt ttatttatt aaaataact gtttcatcg 
      cgatgcgat tccttccaa aatgttaca ataaaagtg 
      caccccgat cggggaaac ccctacgac actaccatc 
      actgggtgc atcgaacgc atagctttc gagaacgtc 
      atcactgaa gcccacatc aagcaccag aaaatacca 
      aaattgggc cgagactcc gtaagaaac atgaaacat 
      ctccataca atcacattc gataactgc gaaattaaa 
      agcatcgat cctgtggct ttccgaaac gtgccaaac 
      ctcaaaacg atcgagatc ctaaatgga aacctaacg 
      tatgtgtct gcaggcgtg tttttgttg gcgccgaaa 
      ttagaaaca ttaagtttg agtcacaac aaattgaga 
      caaattgaa gacggtacg ttcagtgga gtaagcaac 
      ttaaaacgg ctttcttgc gagtccaac aacttggga 
      atatggcgg ccacagtgg ttcacgaat agcacaaat 
      ctgcaaatc ataaatttc caatttaac aaaatcaaa 
      attttgcca aggcgggcg ttcttcgag ctccgaaag 
      ctggaagcg atttatttc aactacaat gaactggaa 
      gttatccaa ccggaagct tttaaaggc atcactcat 
      ttgaaatat ttggggttg agatacaac agactcaaa 
      actatagat cctagaagc ttcccgaac gtgattatg 
      attgattca ttgactatc gccgcaaat tatttgaac 
      gcggtgtcc acgaaattg tggatcagt gttaaaacc 
      aaaaatgtg aatttggac gggaacccg tggaaatgc 
      ctctgttta gatcaagtg cagctccag ctgcacgaa 
      atgaatgca actgttcaa acgtcagat ggttgtgtc 
      tcgggggat gtcccggtc tgttctttt ccggctgtc 
      cactcaaca acttgcaat gaaacggtt gatgatgat 
      cttacgaac acatatttc gcggaactg aggaaggta 
      aacactccc ttggacaag tattgcgcg agattggat 
      taa
    • >TC033821 RB
      atgccgccg caccgttcc gacgctgtc gtcttcggc 
      ggcggcgcc gtagcgtcg cggcgggct cgcttcgac 
      agccctagc aactccatg tcgagtcca ggcgacgcg 
      gccgagccg gctccaccg ccgccggac ccccaggtg 
      gtgcgccgc gaaccgccg ccgtccgag cgcccgccg 
      cggccggtg tcgtaccct cgcgggccg gagagctcc 
      ccccgcacc ggcggcttc gtcaaacgc aactcggac 
      cccgcgccc gcggccgcc ctcaggccc cgcacccag 
      gtcaagccc ttcaccaag gagtcgctc gagcggctc 
      gagaggaaa actgtgcaa ctggtgcgc gagtacggc 
      ttccagccg aggaggaaa ctcagtgtt gaggatggc 
      tcgcggctg cccgccaag ttcgagccc ttccccgcc 
      aaactctac ggcaggccg cttgaggaa atagacaat 
      ttcatttat gacgagacc ttctgcgtg gtttcgaag 
      aggtttcgc aagaactac atccaccgt ttcaccgcc 
      accaattcg ttcttcttc ttcagtccg tttagttca 
      gttaggaaa gcgtgtata tacctgtcc accaaccag 
      tacttcgat tatttagtt atggctact attttgctc 
      aactgcgtc ttccttgcc atggcggaa actattgaa 
      gaagccgag tatattttt ttagccata tatacggct 
      gaaatgata ataaagagc atcgctaaa ggttttata 
      ttaaataag tacacatat ctaaggaat ccatggaat 
      tggttggac ttcgtcgtc atcaccagc ggctacgcc 
      accatcggc atggaggtg gggaacctc gccggtctg 
      cgaacgttt cgcgtctta cgagccctc aagaccatc 
      tcgattctc ccagtactt ttcacgtgc accatgcag 
      cgagtcttg accttaact gtggtttta tttattaaa 
      ataactgtt tcatcgcga tgcgattcc ttccaaaat 
      gttacaata aaagtgcac cccgatcgg ggaaacccc 
      tacgacact accatcact gggtgcatc gaacgcata 
      gctttcgag aacgtcatc actgaagcc cacatcaag 
      caccagaaa ataccaaaa ttgggccga gactccgta 
      agaaacatg aaacatctc catacaatc acattcgat 
      aactgcgaa attaaaagc atcgatcct gtggctttc 
      cgaaacgtg ccaaacctc aaaacgatc gagatccta 
      aatggaaac ctaacgtat gtgtctgca ggcgtgttt 
      ttgttggcg ccgaaatta gaaacatta agtttgagt 
      cacaacaaa ttgagacaa attgaagac ggtacgttc 
      agtggagta agcaactta aaacggctt tcttgcgag 
      tccaacaac ttgggaata tggcggcca cagtggttc 
      acgaatagc acaaatctg caaatcata aatttccaa 
      tttaacaaa atcaaaatt ttgccaagg cgggcgttc 
      ttcgagctc cgaaagctg gaagcgatt tatttcaac 
      tacaatgaa ctggaagtt atccaaccg gaagctttt 
      aaaggcatc actcatttg aaatatttg gggttgaga 
      tacaacaga ctcaaaact atagatcct agaagcttc 
      ccgaacgtg attatgatt gattcattg actatcgcc 
      gcaaattat ttgaacgcg gtgtccacg aaattgtgg 
      atcagtgtt aaaaccaaa aatgtgaat ttggacggg 
      aacccgtgg aaatgcctc tgtttagat caagtgcag 
      ctccagctg cacgaaatg aatgcaact gttcaaacg 
      tcagatggt tgtgtctcg ggggatgtc ccggtctgt 
      tcttttccg gctgtccac tcaacaact tgcaatgaa 
      acggttgat gatgatctt acgaacaca tatttcgcg 
      gaactgagg aaggtaaac actcccttg gacaagtat 
      tgcgcgaga ttggattaa
    mRNA sequence
    • >TC033821 RA
      ttagggagc acacgtaaa gtcgtttac cgagtactc 
      aggtagtaa gtgagtcgc tttaaaata atctaaggg 
      atctgtgga gttgtttcc tccgggtcg agtccggca 
      tgccgccgc accgttccg acgctgtcg tcttcggcg 
      gcggcgccg tagcgtcgc ggcgggctc gcttcgaca 
      gtctagcgc gagattttc gctatgccg cttcgcgtt 
      ccgggcctc aggccctag caactccat gtcgagtcc 
      aggcgacgc ggccgagcc ggctccacc gccgccgga 
      cccccaggt ggtgcgccg cgaaccgcc gccgtccga 
      gcgcccgcc gcggccggt gtcgtaccc tcgcgggcc 
      ggagagctc cccccgcac cggcggctt cgtcaaacg 
      caactcgga ccccgcgcc cgcggccgc cctcaggcc 
      ccgcaccca ggtcaagcc cttcaccaa ggagtcgct 
      cgagcggct cgagaggaa aactgtgca actggtgcg 
      cgagtacgg cttccagcc gaggaggaa actcagtgt 
      tgaggatgg ctcgcggct gcccgccaa gttcgagcc 
      cttccccgc caaactcta cggcaggcc gcttgagga 
      aatagacaa tttcattta tgacgagac cttctgcgt 
      ggtttcgaa gaggtttcg caagaacta catccaccg 
      tttcaccgc caccaattc gttcttctt cttcagtcc 
      gtttagttc agttaggaa agcgtgtat atacctgtc 
      caccaacca gtacttcga ttatttagt tatggctac 
      tattttgct caactgcgt cttccttgc catggcgga 
      aactattga agaagccga gtatatttt tttagccat 
      atatacggc tgaaatgat aataaagag catcgctaa 
      aggttttat attaaataa gtacacata tctaaggaa 
      tccatggaa ttggttgga cttcgtcgt catcaccag 
      cggctacgc caccatcgg catggaggt ggggaacct 
      cgccggtct gcgaacgtt tcgcgtctt acgagccct 
      caagaccat ctcgattct cccagtact tttcacgtg 
      caccatgca gcgagtctt gaccttaac tgtggtttt 
      atttattaa aataactgt ttcatcgcg atgcgattc 
      cttccaaaa tgttacaat aaaagtgca ccccgatcg 
      gggaaaccc ctacgacac taccatcac tgggtgcat 
      cgaacgcat agctttcga gaacgtcat cactgaagc 
      ccacatcaa gcaccagaa aataccaaa attgggccg 
      agactccgt aagaaacat gaaacatct ccatacaat 
      cacattcga taactgcga aattaaaag catcgatcc 
      tgtggcttt ccgaaacgt gccaaacct caaaacgat 
      cgagatcct aaatggaaa cctaacgta tgtgtctgc 
      aggcgtgtt tttgttggc gccgaaatt agaaacatt 
      aagtttgag tcacaacaa attgagaca aattgaaga 
      cggtacgtt cagtggagt aagcaactt aaaacggct 
      ttcttgcga gtccaacaa cttgggaat atggcggcc 
      acagtggtt cacgaatag cacaaatct gcaaatcat 
      aaatttcca atttaacaa aatcaaaat tttgccaag 
      gcgggcgtt cttcgagct ccgaaagct ggaagcgat 
      ttatttcaa ctacaatga actggaagt tatccaacc 
      ggaagcttt taaaggcat cactcattt gaaatattt 
      ggggttgag atacaacag actcaaaac tatagatcc 
      tagaagctt cccgaacgt gattatgat tgattcatt 
      gactatcgc cgcaaatta tttgaacgc ggtgtccac 
      gaaattgtg gatcagtgt taaaaccaa aaatgtgaa 
      tttggacgg gaacccgtg gaaatgcct ctgtttaga 
      tcaagtgca gctccagct gcacgaaat gaatgcaac 
      tgttcaaac gtcagatgg ttgtgtctc gggggatgt 
      cccggtctg ttcttttcc ggctgtcca ctcaacaac 
      ttgcaatga aacggttga tgatgatct tacgaacac 
      atatttcgc ggaactgag gaaggtaaa cactccctt 
      ggacaagta ttgcgcgag attggatta actttcaat 
      tcaattaca aatatactc atacttatt tatttatga 
      aaataaacg tgattcaag cta
    • >TC033821 RB
      ttagggagc acacgtaaa gtcgtttac cgagtactc 
      aggtagtaa gtgagtcgc tttaaaata atctaaggg 
      atctgtgga gttgtttcc tccgggtcg agtccggca 
      tgccgccgc accgttccg acgctgtcg tcttcggcg 
      gcggcgccg tagcgtcgc ggcgggctc gcttcgaca 
      gccctagca actccatgt cgagtccag gcgacgcgg 
      ccgagccgg ctccaccgc cgccggacc cccaggtgg 
      tgcgccgcg aaccgccgc cgtccgagc gcccgccgc 
      ggccggtgt cgtaccctc gcgggccgg agagctccc 
      cccgcaccg gcggcttcg tcaaacgca actcggacc 
      ccgcgcccg cggccgccc tcaggcccc gcacccagg 
      tcaagccct tcaccaagg agtcgctcg agcggctcg 
      agaggaaaa ctgtgcaac tggtgcgcg agtacggct 
      tccagccga ggaggaaac tcagtgttg aggatggct 
      cgcggctgc ccgccaagt tcgagccct tccccgcca 
      aactctacg gcaggccgc ttgaggaaa tagacaatt 
      tcatttatg acgagacct tctgcgtgg tttcgaaga 
      ggtttcgca agaactaca tccaccgtt tcaccgcca 
      ccaattcgt tcttcttct tcagtccgt ttagttcag 
      ttaggaaag cgtgtatat acctgtcca ccaaccagt 
      acttcgatt atttagtta tggctacta ttttgctca 
      actgcgtct tccttgcca tggcggaaa ctattgaag 
      aagccgagt atatttttt tagccatat atacggctg 
      aaatgataa taaagagca tcgctaaag gttttatat 
      taaataagt acacatatc taaggaatc catggaatt 
      ggttggact tcgtcgtca tcaccagcg gctacgcca 
      ccatcggca tggaggtgg ggaacctcg ccggtctgc 
      gaacgtttc gcgtcttac gagccctca agaccatct 
      cgattctcc cagtacttt tcacgtgca ccatgcagc 
      gagtcttga ccttaactg tggttttat ttattaaaa 
      taactgttt catcgcgat gcgattcct tccaaaatg 
      ttacaataa aagtgcacc ccgatcggg gaaacccct 
      acgacacta ccatcactg ggtgcatcg aacgcatag 
      ctttcgaga acgtcatca ctgaagccc acatcaagc 
      accagaaaa taccaaaat tgggccgag actccgtaa 
      gaaacatga aacatctcc atacaatca cattcgata 
      actgcgaaa ttaaaagca tcgatcctg tggctttcc 
      gaaacgtgc caaacctca aaacgatcg agatcctaa 
      atggaaacc taacgtatg tgtctgcag gcgtgtttt 
      tgttggcgc cgaaattag aaacattaa gtttgagtc 
      acaacaaat tgagacaaa ttgaagacg gtacgttca 
      gtggagtaa gcaacttaa aacggcttt cttgcgagt 
      ccaacaact tgggaatat ggcggccac agtggttca 
      cgaatagca caaatctgc aaatcataa atttccaat 
      ttaacaaaa tcaaaattt tgccaaggc gggcgttct 
      tcgagctcc gaaagctgg aagcgattt atttcaact 
      acaatgaac tggaagtta tccaaccgg aagctttta 
      aaggcatca ctcatttga aatatttgg ggttgagat 
      acaacagac tcaaaacta tagatccta gaagcttcc 
      cgaacgtga ttatgattg attcattga ctatcgccg 
      caaattatt tgaacgcgg tgtccacga aattgtgga 
      tcagtgtta aaaccaaaa atgtgaatt tggacggga 
      acccgtgga aatgcctct gtttagatc aagtgcagc 
      tccagctgc acgaaatga atgcaactg ttcaaacgt 
      cagatggtt gtgtctcgg gggatgtcc cggtctgtt 
      cttttccgg ctgtccact caacaactt gcaatgaaa 
      cggttgatg atgatctta cgaacacat atttcgcgg 
      aactgagga aggtaaaca ctcccttgg acaagtatt 
      gcgcgagat tggattaac tttcaattc aattacaaa 
      tatactcat acttattta tttatgaaa ataaacgtg 
      attcaagct a
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