Status
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Gene information
General information
TC008731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15143477..15148652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04539

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008731 RA
      MSFFYNATP FLIVAVLII GEVYCRDAS SLTRADNLR 
      LPPWDVKNA TQWPALGRP SNPSWFSVR PMTRQRPST 
      NNFNTGTRR QEPQIPQST NEVTDDELR NFAETLLTK 
      DVNNAAKYV TINLQGKTT SGSSRDAAP LPLLSIDKE 
      AFKIASIDK TLRLHDNYI VESNMNEYS SPQEKNEEN 
      SLLDTILTT PVMQETRNF LMRTNRIGR DPNEFKNIL 
      REIWFEMYA RGGGKIGSS GFEHVFLAE IKKNQVSGL 
      HNWLYFEQE ERNNQANYL GYMKKIDLG DKGAILKYH 
      FTFHGIDKP VGSMFIGTS PEFEMALYS TCFLLRADR 
      VCPLKLNGS RFVIRTFTY RYRGKNMIG SAFPDI
    CDS sequence
    • >TC008731 RA
      atgtcgttc ttttataat gcgacccct tttttaatt 
      gtggccgtt ttaattatt ggagaagtt tactgtaga 
      gacgcatct tcgctgaca cgtgcagat aacttgcga 
      ttaccacca tgggatgtt aaaaacgcg actcaatgg 
      ccagcctta ggccgccca agcaaccca tcatggttc 
      agcgtcaga cccatgacc cgccaacgt cccagcact 
      aataatttt aacacaggg acacgtcga caagaaccc 
      caaatacct caatcaacg aatgaagta accgatgac 
      gaattaaga aattttgcc gaaacgttg ttaacaaaa 
      gatgtaaac aatgctgcc aaatatgta acaatcaac 
      ttgcaaggt aaaactact tcgggctcc agtagagat 
      gcggcgcct ttgccactt ctatcaatc gacaaagaa 
      gctttcaaa atcgcatca attgacaaa acgttgcgt 
      ttacacgac aattacatc gtggaaagc aacatgaac 
      gaatactcg agccctcaa gagaaaaac gaagagaat 
      tcgcttttg gacacgatt ctaacaacg ccagtaatg 
      caagagacg cgcaatttt cttatgcgg accaatcgc 
      atcggacga gaccccaac gaattcaaa aacattttg 
      cgcgaaatt tggttcgag atgtatgcg aggggcggc 
      ggtaaaatc ggttcgagt ggcttcgaa catgttttt 
      ttagccgaa attaagaaa aaccaagtg tcgggattg 
      cacaattgg ctgtatttc gagcaagaa gagcgcaac 
      aatcaagcc aattatttg ggatacatg aaaaaaatt 
      gaccttggc gataagggg gcaattttg aaataccat 
      ttcacattt catggcatt gataagccg gttgggtcg 
      atgtttatt ggaacgagc cccgaattt gaaatggct 
      ttgtattcg acgtgtttt ttgctgaga gcagaccga 
      gtttgtcct ttgaaattg aatggaagc aggttcgtt 
      ataaggacc tttacctac aggtacagg gggaagaat 
      atgatcggg agtgccttc cctgatatt tga
    mRNA sequence
    • >TC008731 RA
      cagttataa attttgaac gtgaaacat gtcaaacat 
      gactttatc acataaaca tttattcca ttaaagcaa 
      ttctcgtcg agttaaaac aatgccaca acaatgaat 
      aaccaagtt tcaaatcat tttgacatt tttccatgt 
      catccagac gtaaaaaat tgtgaaaaa tgggaatta 
      catgaataa tagtgacga aacttgatt agagtaagt 
      aatgatgtt ttttggtac caaatttca attgccacg 
      tcttgaaat cgggttttg acggtcagt ggtgatata 
      acaaaatgg tatttgtgc gatttgtgt aaaattatt 
      gcgctggcc ttcaggtaa aaatggaga taaacagtt 
      tgtcgcgtt ttattataa aatttatgc ccggttatt 
      gtaatttaa cgtaaccgt agatcgtta aataatacg 
      acaggtatt gaaaaaatc ggtgtgaaa gtaatgcag 
      aaacgggag aaaatgttt ccttcggtc aatctttca 
      ctggctgca atgtgcaaa cgatgatgt aatttgcga 
      gtgtcgtga ttcaaagtg aaaacagtt tgatggaag 
      gtttgtaca tcacttttt ttaacactg aataatgca 
      tttattatt attacaaac attgttttg ttgtttttc 
      ccacctatc tttgctgat aagataaat ttacgagta 
      gttcccatt tttgagctt atctttcaa ccacccatc 
      tcttgtatc tatttattt gtaaaaagg ttgaaccgc 
      cagaaataa gatacggct tatctgagg tttagtttc 
      cattgaaaa aattacgag cgatagtca agcaatgtt 
      ttcacactc cattccgca ccaccatgc aaccatcca 
      ataattatt aaacaatcg tcaccaaaa tgttgtgtt 
      gtgtaaagt ttaaaaatg ggtaaaatc gagcaattt 
      atgtgcttt ttcacagat ggacaatcc cagttttcg 
      gaaagatgg tgacaggag ggataaagg tatagaatt 
      atcggctcc gagggcaaa tgggggcgc ttcggccag 
      ccaaaattt agtcacctg tacgcctga agtatgcag 
      aagtgcaag acaacgata ccaagcccc ggatctcgc 
      cggtagccc ctggcacat aaaaaagac tatctttct 
      cgtcggaat aatagtgct tttaattgt ttggattat 
      cgtttttac cactttaaa tcaattcaa actgttgtg 
      atcgtgtgt agtctaata agtgaagaa acagcaaat 
      gtgtgtgtt gtttaattt taaggtgca atgaataca 
      atattttta ttcgagttg ttgcgtttt gttcaatga 
      attatttat aaccggaat tgaataact ctctcaagg 
      taactcgcc acaagtggg taaaacaaa acaacgaca 
      cgacgaaat cagtctcaa tcagattaa acaacttgt 
      tatcatcaa actttcaac tatctgttc gtttttaac 
      gttcgaaca atgaacacc agtacggaa attctaaca 
      aagcgccat acagaggtg atggagcaa ggggcttcc 
      gcaaggaaa ccgcaagtg cagcaagtt tttgttggg 
      tgggacaga caaaagtcg ttcacattc cttggactt 
      tcactctgt ttcgttgtt tgggcctgc gtgtacttc 
      cccttgatg tcataacat aacactttc acttattgt 
      aaataatgt aaattctgt tttaataaa attataatt 
      aattgtggg ttgcctaac ttgccttca agattaatt 
      tgcacatct cttccgctc caaggtttt atcattgaa 
      ttaaattag gcaaaaaag tgttatgaa taatatgat 
      tgtgtgcct gttgaactt aagacatgt cgttctttt 
      ataatgcga ccccttttt taattgtgg ccgttttaa 
      ttattggag aagtttact gtagagacg catcttcgc 
      tgacacgtg cagataact tgcgattac caccatggg 
      atgttaaaa acgcgactc aatggccag ccttaggcc 
      gcccaagca acccatcat ggttcagcg tcagaccca 
      tgacccgcc aacgtccca gcactaata attttaaca 
      cagggacac gtcgacaag aaccccaaa tacctcaat 
      caacgaatg aagtaaccg atgacgaat taagaaatt 
      ttgccgaaa cgttgttaa caaaagatg taaacaatg 
      ctgccaaat atgtaacaa tcaacttgc aaggtaaaa 
      ctacttcgg gctccagta gagatgcgg cgcctttgc 
      cacttctat caatcgaca aagaagctt tcaaaatcg 
      catcaattg acaaaacgt tgcgtttac acgacaatt 
      acatcgtgg aaagcaaca tgaacgaat actcgagcc 
      ctcaagaga aaaacgaag agaattcgc ttttggaca 
      cgattctaa caacgccag taatgcaag agacgcgca 
      attttctta tgcggacca atcgcatcg gacgagacc 
      ccaacgaat tcaaaaaca ttttgcgcg aaatttggt 
      tcgagatgt atgcgaggg gcggcggta aaatcggtt 
      cgagtggct tcgaacatg tttttttag ccgaaatta 
      agaaaaacc aagtgtcgg gattgcaca attggctgt 
      atttcgagc aagaagagc gcaacaatc aagccaatt 
      atttgggat acatgaaaa aaattgacc ttggcgata 
      agggggcaa ttttgaaat accatttca catttcatg 
      gcattgata agccggttg ggtcgatgt ttattggaa 
      cgagccccg aatttgaaa tggctttgt attcgacgt 
      gttttttgc tgagagcag accgagttt gtcctttga 
      aattgaatg gaagcaggt tcgttataa ggaccttta 
      cctacaggt acaggggga agaatatga tcgggagtg 
      ccttccctg atatttgat ctaatttcc attatcatt 
      atattgatc aagtgtttg caataactg agataaaga 
      ttgtttcaa catgcgaat aaataagat aaaacaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY