Status
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Gene information
General information
TC033871
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008724,TC008725
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15090533..15095340
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033871
    iBeetle screen iB_04538

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033871 RA
      MYGDELITL FAKLNVRIG DLEPRVIKA WFDTDCKDL 
      LEWICASIT EDCYISPQE EHEYSRLEN VLTGDKLEK 
      IVKAIELEY PDLFQVETN EVDIEIAND IIEVLEEQL 
      EGCENQLNI NQRLKSNLA AEYSEVSSQ LIKSQLELE 
      ETQEKCMKL SQELDRVNK ETHTQVVKY ASALDDFES 
      AQHPKFINN GDITEFEEK FDHVVDILR TFMISNNYS 
      LRLGDDMLS EIKNRIFYS TLSRLKLEM EVEAQHSVL 
      KFYETLNLY SLNSLAFDC NFTQMEIEE NKDCQNQMM 
      VNVEKLLMK YAEQCVNEP SLRFYKNEL NFYVSRLEN 
      LNVVEERLK KILVNFNLM HNMFLQEKM DIQYSSSFL 
      KDMHKYIQE NLKQCYVRK QKMLKKIDE YEKFSIKPV 
      EEKLKLVRD FQIVLSKDV NTLQKTLQE STKLKIETT 
      RLKNKHFNF AQCFKSCLQ SRHNVEILR NFLICGPTF 
      QIVTIPHGL QMLLFQVDQ TLAKLQTST KMVVAAARK 
      PKELVAKDK WLRYRNQLW MLFMTDPEK MKRFLCQFA 
      ENENCEITK LSQTSKFGL TFLE
    CDS sequence
    • >TC033871 RA
      atgtatgga gatgaacta ataaccctt tttgcgaaa 
      cttaatgta agaatcggt gatttggag ccccgtgtc 
      ataaaagcc tggttcgat acagattgt aaagatttg 
      ctagaatgg atttgtgcc tcaatcacc gaagattgc 
      tacatctcg cctcaagaa gaacacgaa tactctcgg 
      cttgagaac gttttaact ggtgataag ttggaaaaa 
      atagttaaa gcaatcgag ttagaatat cccgacttg 
      tttcaagtt gagacaaat gaggtagat atagaaatt 
      gccaatgac ataattgag gtgttggag gaacagttg 
      gaagggtgt gaaaaccag ttaaacatc aatcagcga 
      ttaaaatcc aatttggcc gcggaatat tccgaagta 
      tcttctcag ttaatcaag tcgcagctt gaactggag 
      gagacgcaa gaaaaatgc atgaagttg agtcaggaa 
      ctcgaccga gttaacaag gaaacacac actcaagtg 
      gtaaagtat gcttcggcc ttggatgac ttcgaatct 
      gcacaacat ccgaaattt attaacaac ggcgatata 
      acagaattt gaggagaag tttgatcat gttgttgat 
      attttacgc acttttatg ataagcaat aactattcc 
      ttaaggctc ggagatgat atgctttca gaaattaaa 
      aaccgcatt ttttattcc accttaagc cgtctgaag 
      ctagaaatg gaagtggaa gcacaacat tcagtttta 
      aaattctac gaaactctt aatctctac tcattaaat 
      tcgttggca tttgattgt aactttact caaatggaa 
      atcgaagag aataaagac tgtcagaac caaatgatg 
      gtaaatgtg gagaagttg ttaatgaaa tatgctgaa 
      caatgcgtt aatgaaccg agtttaagg ttttataaa 
      aacgagcta aacttttat gtttctaga ttagaaaac 
      ttgaatgta gttgaggaa agactgaaa aaaattttg 
      gttaatttt aatttaatg cacaatatg tttttacaa 
      gaaaagatg gacatacag tattcgagt agtttccta 
      aaagatatg cacaaatat atccaggag aacttgaag 
      cagtgttat gttcgaaag caaaaaatg ttgaagaaa 
      atcgatgaa tatgaaaag ttttcgatt aaaccggtg 
      gaagagaaa cttaaattg gtacgtgat tttcaaatt 
      gttctctcc aaggatgta aacactcta caaaaaact 
      ttacaagaa agcacgaag ttgaaaatt gaaacaacg 
      cgcttgaaa aacaaacac tttaatttt gctcagtgc 
      tttaagagt tgcctccaa tcaaggcac aacgttgaa 
      attctacga aacttccta atttgcgga cctactttc 
      cagattgtt accatacca catggttta caaatgtta 
      cttttccaa gttgaccaa actttagcc aaattacaa 
      acttcaacg aaaatggtg gtagcagca gcacggaaa 
      ccaaaagaa ctcgtcgca aaagataaa tggttgcgt 
      tatcgaaac cagttgtgg atgcttttc atgactgat 
      ccggagaag atgaaacgt tttctgtgt caatttgcc 
      gaaaatgaa aactgtgaa attaccaag ctttcccaa 
      acttcgaag tttgggtta acttttttg gaataa
    mRNA sequence
    • >TC033871 RA
      caaaacaaa aacgatttt tgtgctttg taaatattt 
      tttccgttc gtgtttttc atacagtta atttgtttt 
      tatggtgcg aaaccggcg gcacaataa tgtatggag 
      atgaactaa taacccttt ttgcgaaac ttaatgtaa 
      gaatcggtg atttggagc cccgtgtca taaaagcct 
      ggttcgata cagattgta aagatttgc tagaatgga 
      tttgtgcct caatcaccg aagattgct acatctcgc 
      ctcaagaag aacacgaat actctcggc ttgagaacg 
      ttttaactg gtgataagt tggaaaaaa tagttaaag 
      caatcgagt tagaatatc ccgacttgt ttcaagttg 
      agacaaatg aggtagata tagaaattg ccaatgaca 
      taattgagg tgttggagg aacagttgg aagggtgtg 
      aaaaccagt taaacatca atcagcgat taaaatcca 
      atttggccg cggaatatt ccgaagtat cttctcagt 
      taatcaagt cgcagcttg aactggagg agacgcaag 
      aaaaatgca tgaagttga gtcaggaac tcgaccgag 
      ttaacaagg aaacacaca ctcaagtgg taaagtatg 
      cttcggcct tggatgact tcgaatctg cacaacatc 
      cgaaattta ttaacaacg gcgatataa cagaatttg 
      aggagaagt ttgatcatg ttgttgata ttttacgca 
      cttttatga taagcaata actattcct taaggctcg 
      gagatgata tgctttcag aaattaaaa accgcattt 
      tttattcca ccttaagcc gtctgaagc tagaaatgg 
      aagtggaag cacaacatt cagttttaa aattctacg 
      aaactctta atctctact cattaaatt cgttggcat 
      ttgattgta actttactc aaatggaaa tcgaagaga 
      ataaagact gtcagaacc aaatgatgg taaatgtgg 
      agaagttgt taatgaaat atgctgaac aatgcgtta 
      atgaaccga gtttaaggt tttataaaa acgagctaa 
      acttttatg tttctagat tagaaaact tgaatgtag 
      ttgaggaaa gactgaaaa aaattttgg ttaatttta 
      atttaatgc acaatatgt ttttacaag aaaagatgg 
      acatacagt attcgagta gtttcctaa aagatatgc 
      acaaatata tccaggaga acttgaagc agtgttatg 
      ttcgaaagc aaaaaatgt tgaagaaaa tcgatgaat 
      atgaaaagt tttcgatta aaccggtgg aagagaaac 
      ttaaattgg tacgtgatt ttcaaattg ttctctcca 
      aggatgtaa acactctac aaaaaactt tacaagaaa 
      gcacgaagt tgaaaattg aaacaacgc gcttgaaaa 
      acaaacact ttaattttg ctcagtgct ttaagagtt 
      gcctccaat caaggcaca acgttgaaa ttctacgaa 
      acttcctaa tttgcggac ctactttcc agattgtta 
      ccataccac atggtttac aaatgttac ttttccaag 
      ttgaccaaa ctttagcca aattacaaa cttcaacga 
      aaatggtgg tagcagcag cacggaaac caaaagaac 
      tcgtcgcaa aagataaat ggttgcgtt atcgaaacc 
      agttgtgga tgcttttca tgactgatc cggagaaga 
      tgaaacgtt ttctgtgtc aatttgccg aaaatgaaa 
      actgtgaaa ttaccaagc tttcccaaa cttcgaagt 
      ttgggttaa cttttttgg aataattgt tacattaaa 
      cttatttaa taataaata tcttgttca tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY