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Gene information
General information
TC033868
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008720,TC008719
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14894384..14981544
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033868
    iBeetle screen iB_04537

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    Pupal morphology
    female genital lobes rounded - Number of animals showing the phenotype: 10

    Adult morphology
    club distalelongated - Number of animals showing the phenotype: 9

    Comment: last club segment elongated

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033868 RA
      MEMDPQERE SDLGDDSPV SHNDEEISD GDGESGREH 
      DDVDSCDEI RRHGGGILA DHGDVLAKL KMQVRDIKD 
      DFENATRHL NFPLTNRSL QDNFHLPAP FPFPHPSTP 
      FMTPLLPPP AAPSSGSSH SSEGSASSQ HTWSFEEQF 
      KQVRQWHRR LISFLFQLY EINDDPKRK EFLDDLFSF 
      MQKRGTPIN RLPIMAKSV LDLYELYNL VIARGGLVD 
      VINKKLWQE IIKGLHLPS SITSAAFTL RTQYMKYLY 
      PYECEKRRL STPAELQAA IDGNRREGR RSSYGQYDS 
      MQRSPNPSQ MSPLSLVSQ QQQLAARMN SVMSGGMAL 
      HNGAHHPSH PQPLGQPLN GGPLPGLAP SEFEARMME 
      YVKLLNKEI RSSAATPPR QGDVSPPNA TSPLNQLEL 
      SRITLWNMY NNNQPPVEP QKEALNLSD PTQPSVKRE 
      PEHRDSPPP PKKFSKDDD EQKISPATH IKINSRGES 
      RDARNGDSS LVVSMELNG ITYQGVLFA QSSPSSGRT 
      AMAS
    • >TC033868 RB
      MEMDPQERE SDLGDDSPV SHNDEEISD GDGESGREH 
      DDVDSCDEI RRHGGGILA DHGDVLAKL KMQVRDIKV 
      GDDFENATR HLNFPLTNR SLQDNFHLP APFPFPHPS 
      TPFMTPLLP PPAAPSSGS SHSSEGSAS SQHTWSFEE 
      QFKQVRQWH RRLISFLFQ LYEINDDPK RKEFLDDLF 
      SFMQKRGTP INRLPIMAK SVLDLYELY NLVIARGGL 
      VDVINKKLW QEIIKGLHL PSSITSAAF TLRTQYMKY 
      LYPYECEKR RLSTPAELQ AAIDGNRRE GRRSSYGQY 
      DSMQRSPNP SQMSPLSLV SQQQQLAAR MNSVMSGGM 
      ALHNGAHHP SHPQPLGQP LNGGPLPGL APSEFEARM 
      MEYVKLLNK EIRSSAATP PRQGDVSPP NATSPLNQL 
      ELSRITLWN MYNNNQPPV EPQKEALNL SDPTQPSVK 
      REPEHRDSP PPPKKFSKD DDEQKISPA THIKINSRG 
      ESRDARNGD SSLVVSMEL NGITYQGVL FAQSSPSSG 
      RTAMAS
    CDS sequence
    • >TC033868 RA
      atggaaatg gatccgcaa gaacgggaa agtgatctg 
      ggggatgat agtccggtg tcccacaat gacgaagaa 
      ataagtgac ggtgacgga gagtcgggc cgcgaacat 
      gacgatgtt gatagctgt gatgagata aggaggcac 
      gggggcgga attctggca gatcacgga gatgttctt 
      gcaaagttg aaaatgcaa gttagggac atcaaggac 
      gacttcgaa aacgccacc cgccacctg aatttcccc 
      ctcacaaat cgaagcctg caggataat ttccatttg 
      ccggctccg tttccattt ccacacccg agtacaccg 
      ttcatgaca cctctttta ccaccacca gcagctcca 
      tcttcgggg agttcacac tcgtcggaa ggtagtgca 
      tcctcacag cacacctgg tcgttcgaa gaacaattc 
      aaacaggtg cgtcagtgg catcgccga ttaattagt 
      tttttgttt cagttgtat gaaatcaac gacgacccc 
      aaacggaaa gaattcctc gacgatctc ttctccttc 
      atgcagaaa cggggtact ccgatcaat cgactgccc 
      atcatggcc aaatccgtt ctcgattta tacgaattg 
      tacaattta gtcatcgcg aggggtgga cttgttgat 
      gtcatcaat aagaaactc tggcaggaa attatcaaa 
      gggctgcat ctaccgtcg tcgataacg tcggcagct 
      ttcacactc aggacgcaa tacatgaaa tacctgtac 
      ccttacgaa tgcgagaaa cgccgcctc agcacaccg 
      gccgagctc caggcggcc atcgacggc aaccgtcgc 
      gaaggccgc cgcagcagc tacggccag tacgactcc 
      atgcagcgc tcccccaac ccttcgcag atgtctcct 
      ttgtccctc gtctcccag cagcaacaa ctcgccgcc 
      cgcatgaac tccgtcatg agcggtggc atggccctc 
      cacaacggc gcccaccac cccagccac ccccagccc 
      ttgggtcag ccgctcaac ggcggcccg ctccctggc 
      ctcgccccc agcgagttc gaggcgcgc atgatggaa 
      tacgtcaag ctcctcaac aaggaaatc cgcagctcg 
      gcggctact ccgccacga caaggggac gtgtcgccc 
      cctaacgcc acctcgcca ctcaaccag ctggagctg 
      tcgcggata acgctctgg aatatgtac aataataat 
      caacccccc gtcgagccg caaaaggaa gcgttgaat 
      ctctccgac ccgacgcag ccttcggtg aaacgggag 
      ccggagcat agagattcg ccaccgccc ccgaagaag 
      ttttccaaa gatgatgat gaacagaag atctcgccc 
      gccacgcat atcaagatt aacagtaga ggtgagtct 
      agggatgcg cgtaacgga gacagttcc ttagtagtc 
      agtatggaa ttaaatgga attacatat caaggggtc 
      ctattcgcg cagtcttcg ccatctagc ggccgaacg 
      gcgatggcc tcgtaa
    • >TC033868 RB
      atggaaatg gatccgcaa gaacgggaa agtgatctg 
      ggggatgat agtccggtg tcccacaat gacgaagaa 
      ataagtgac ggtgacgga gagtcgggc cgcgaacat 
      gacgatgtt gatagctgt gatgagata aggaggcac 
      gggggcgga attctggca gatcacgga gatgttctt 
      gcaaagttg aaaatgcaa gttagggac atcaaggta 
      ggggacgac ttcgaaaac gccacccgc cacctgaat 
      ttccccctc acaaatcga agcctgcag gataatttc 
      catttgccg gctccgttt ccatttcca cacccgagt 
      acaccgttc atgacacct cttttacca ccaccagca 
      gctccatct tcggggagt tcacactcg tcggaaggt 
      agtgcatcc tcacagcac acctggtcg ttcgaagaa 
      caattcaaa caggtgcgt cagtggcat cgccgatta 
      attagtttt ttgtttcag ttgtatgaa atcaacgac 
      gaccccaaa cggaaagaa ttcctcgac gatctcttc 
      tccttcatg cagaaacgg ggtactccg atcaatcga 
      ctgcccatc atggccaaa tccgttctc gatttatac 
      gaattgtac aatttagtc atcgcgagg ggtggactt 
      gttgatgtc atcaataag aaactctgg caggaaatt 
      atcaaaggg ctgcatcta ccgtcgtcg ataacgtcg 
      gcagctttc acactcagg acgcaatac atgaaatac 
      ctgtaccct tacgaatgc gagaaacgc cgcctcagc 
      acaccggcc gagctccag gcggccatc gacggcaac 
      cgtcgcgaa ggccgccgc agcagctac ggccagtac 
      gactccatg cagcgctcc cccaaccct tcgcagatg 
      tctcctttg tccctcgtc tcccagcag caacaactc 
      gccgcccgc atgaactcc gtcatgagc ggtggcatg 
      gccctccac aacggcgcc caccacccc agccacccc 
      cagcccttg ggtcagccg ctcaacggc ggcccgctc 
      cctggcctc gcccccagc gagttcgag gcgcgcatg 
      atggaatac gtcaagctc ctcaacaag gaaatccgc 
      agctcggcg gctactccg ccacgacaa ggggacgtg 
      tcgccccct aacgccacc tcgccactc aaccagctg 
      gagctgtcg cggataacg ctctggaat atgtacaat 
      aataatcaa ccccccgtc gagccgcaa aaggaagcg 
      ttgaatctc tccgacccg acgcagcct tcggtgaaa 
      cgggagccg gagcataga gattcgcca ccgcccccg 
      aagaagttt tccaaagat gatgatgaa cagaagatc 
      tcgcccgcc acgcatatc aagattaac agtagaggt 
      gagtctagg gatgcgcgt aacggagac agttcctta 
      gtagtcagt atggaatta aatggaatt acatatcaa 
      ggggtccta ttcgcgcag tcttcgcca tctagcggc 
      cgaacggcg atggcctcg taa
    mRNA sequence
    • >TC033868 RA
      ttggagatg tgcgctagc gtttgttgg tagtgtttc 
      tcgcctagc acacttgac cagaattga acgctgaaa 
      gcacatgac ataaccaaa cttttcgcg ggtgtttac 
      gtgagtgtg tttcggttt acgtttttt ttcggtgtg 
      gtgtgcaga gtgctttga gcattctgc aaaagacaa 
      tttagcaaa attcgcgtc aaaaacaga gaacccgtt 
      cccgtttat gccatggaa atggatccg caagaacgg 
      gaaagtgat ctgggggat gatagtccg gtgtcccac 
      aatgacgaa gaaataagt gacggtgac ggagagtcg 
      ggccgcgaa catgacgat gttgatagc tgtgatgag 
      ataaggagg cacgggggc ggaattctg gcagatcac 
      ggagatgtt cttgcaaag ttgaaaatg caagttagg 
      gacatcaag gacgacttc gaaaacgcc acccgccac 
      ctgaatttc cccctcaca aatcgaagc ctgcaggat 
      aatttccat ttgccggct ccgtttcca tttccacac 
      ccgagtaca ccgttcatg acacctctt ttaccacca 
      ccagcagct ccatcttcg gggagttca cactcgtcg 
      gaaggtagt gcatcctca cagcacacc tggtcgttc 
      gaagaacaa ttcaaacag gtgcgtcag tggcatcgc 
      cgattaatt agttttttg tttcagttg tatgaaatc 
      aacgacgac cccaaacgg aaagaattc ctcgacgat 
      ctcttctcc ttcatgcag aaacggggt actccgatc 
      aatcgactg cccatcatg gccaaatcc gttctcgat 
      ttatacgaa ttgtacaat ttagtcatc gcgaggggt 
      ggacttgtt gatgtcatc aataagaaa ctctggcag 
      gaaattatc aaagggctg catctaccg tcgtcgata 
      acgtcggca gctttcaca ctcaggacg caatacatg 
      aaatacctg tacccttac gaatgcgag aaacgccgc 
      ctcagcaca ccggccgag ctccaggcg gccatcgac 
      ggcaaccgt cgcgaaggc cgccgcagc agctacggc 
      cagtacgac tccatgcag cgctccccc aacccttcg 
      cagatgtct cctttgtcc ctcgtctcc cagcagcaa 
      caactcgcc gcccgcatg aactccgtc atgagcggt 
      ggcatggcc ctccacaac ggcgcccac caccccagc 
      cacccccag cccttgggt cagccgctc aacggcggc 
      ccgctccct ggcctcgcc cccagcgag ttcgaggcg 
      cgcatgatg gaatacgtc aagctcctc aacaaggaa 
      atccgcagc tcggcggct actccgcca cgacaaggg 
      gacgtgtcg ccccctaac gccacctcg ccactcaac 
      cagctggag ctgtcgcgg ataacgctc tggaatatg 
      tacaataat aatcaaccc cccgtcgag ccgcaaaag 
      gaagcgttg aatctctcc gacccgacg cagccttcg 
      gtgaaacgg gagccggag catagagat tcgccaccg 
      cccccgaag aagttttcc aaagatgat gatgaacag 
      aagatctcg cccgccacg catatcaag attaacagt 
      agaggtgag tctagggat gcgcgtaac ggagacagt 
      tccttagta gtcagtatg gaattaaat ggaattaca 
      tatcaaggg gtcctattc gcgcagtct tcgccatct 
      agcggccga acggcgatg gcctcgtaa agggaacct 
      ctctgaatg tgatgtaga accctaatt tggcggtct 
      gtgtggcgc caaattgga cacgctttt caattgttt 
      tatatttat tatttttct agttgagtg tttttagtg 
      atctagtgc tccaggaac tatgtgcac tttttataa 
      tatggatgt ttttttttt gtaaagctg taaaatccg 
      taatggcat cgcattaaa tacatgttt aagtacaaa 
      agtaattgt ctaatgtat aaaatagaa taaacgaag 
      tttatggca cattgagcg tagagattt ggcttcatt 
      aggctattg taactgttg gtcgtctcg aaaaatatg 
      gatattatg acataaatc aagagaaac gacagtttt 
      aatgtgcca ccatactta aaacttccc attttgcaa 
      cttagaata cttaaatct acttacgta catggccag 
      tctacaagt cattgttca tttgtctaa ccatgaaac 
      tctttgtgg gaaattatt aattgagta aatttattt 
      tttgagccg ttgtaacaa agtttgaat aatttggtt 
      tcccgcgaa tgcttcatg aggcgcacg tttcaattt 
      ttgtttgaa gatttacaa aaatatctc ggcaacgtt 
      tttgtaaat agctataaa tactttagt atcagtatt 
      tgaggatat attcgtgtg ttatatatt tttttgtat 
      atctaccaa aagactgtt attttatta attttaaat 
      atattatta ggataaaac aggttagac tatttatta 
      agtgatttt agtgtattt atgaaatca attattgtg 
      tagcaatat tagccgtaa gtgtagatt atatgaaaa 
      ttactgtag ttccaaaag tgtagtcct agttcataa 
      atgacatcc gatcatccc tacaaactt tgtgaaaat 
      ttgttaatt tttttgatc tgattatct cagggatat 
      ttgatggta atgtgcaaa acatattcc tgctttcat 
      tgtgttcta gtttttcgt tcaaagttt gtaagtatt 
      taaggcgaa aggccagca ttctaattc aatgtatta 
      ctgtaaata ccaacctat cattaaaca tactcgtaa 
      aagtttttg ttttattac ttcttcagc taaatacct 
      tgtccaaaa aatagctca acatcatta gcattttcc 
      aaaattaaa acaatatat ctcatttta gttg
    • >TC033868 RB
      ttggagatg tgcgctagc gtttgttgg tagtgtttc 
      tcgcctagc acacttgac cagaattga acgctgaaa 
      gcacatgac ataaccaaa cttttcgcg ggtgtttac 
      gtgagtgtg tttcggttt acgtttttt ttcggtgtg 
      gtgtgcaga gtgctttga gcattctgc aaaagacaa 
      tttagcaaa attcgcgtc aaaaacaga gaacccgtt 
      cccgtttat gccatggaa atggatccg caagaacgg 
      gaaagtgat ctgggggat gatagtccg gtgtcccac 
      aatgacgaa gaaataagt gacggtgac ggagagtcg 
      ggccgcgaa catgacgat gttgatagc tgtgatgag 
      ataaggagg cacgggggc ggaattctg gcagatcac 
      ggagatgtt cttgcaaag ttgaaaatg caagttagg 
      gacatcaag gtaggggac gacttcgaa aacgccacc 
      cgccacctg aatttcccc ctcacaaat cgaagcctg 
      caggataat ttccatttg ccggctccg tttccattt 
      ccacacccg agtacaccg ttcatgaca cctctttta 
      ccaccacca gcagctcca tcttcgggg agttcacac 
      tcgtcggaa ggtagtgca tcctcacag cacacctgg 
      tcgttcgaa gaacaattc aaacaggtg cgtcagtgg 
      catcgccga ttaattagt tttttgttt cagttgtat 
      gaaatcaac gacgacccc aaacggaaa gaattcctc 
      gacgatctc ttctccttc atgcagaaa cggggtact 
      ccgatcaat cgactgccc atcatggcc aaatccgtt 
      ctcgattta tacgaattg tacaattta gtcatcgcg 
      aggggtgga cttgttgat gtcatcaat aagaaactc 
      tggcaggaa attatcaaa gggctgcat ctaccgtcg 
      tcgataacg tcggcagct ttcacactc aggacgcaa 
      tacatgaaa tacctgtac ccttacgaa tgcgagaaa 
      cgccgcctc agcacaccg gccgagctc caggcggcc 
      atcgacggc aaccgtcgc gaaggccgc cgcagcagc 
      tacggccag tacgactcc atgcagcgc tcccccaac 
      ccttcgcag atgtctcct ttgtccctc gtctcccag 
      cagcaacaa ctcgccgcc cgcatgaac tccgtcatg 
      agcggtggc atggccctc cacaacggc gcccaccac 
      cccagccac ccccagccc ttgggtcag ccgctcaac 
      ggcggcccg ctccctggc ctcgccccc agcgagttc 
      gaggcgcgc atgatggaa tacgtcaag ctcctcaac 
      aaggaaatc cgcagctcg gcggctact ccgccacga 
      caaggggac gtgtcgccc cctaacgcc acctcgcca 
      ctcaaccag ctggagctg tcgcggata acgctctgg 
      aatatgtac aataataat caacccccc gtcgagccg 
      caaaaggaa gcgttgaat ctctccgac ccgacgcag 
      ccttcggtg aaacgggag ccggagcat agagattcg 
      ccaccgccc ccgaagaag ttttccaaa gatgatgat 
      gaacagaag atctcgccc gccacgcat atcaagatt 
      aacagtaga ggtgagtct agggatgcg cgtaacgga 
      gacagttcc ttagtagtc agtatggaa ttaaatgga 
      attacatat caaggggtc ctattcgcg cagtcttcg 
      ccatctagc ggccgaacg gcgatggcc tcgtaaagg 
      gaacctctc tgaatgtga tgtagaacc ctaatttgg 
      cggtctgtg tggcgccaa attggacac gcttttcaa 
      ttgttttat atttattat ttttctagt tgagtgttt 
      ttagtgatc tagtgctcc aggaactat gtgcacttt 
      ttataatat ggatgtttt ttttttgta aagctgtaa 
      aatccgtaa tggcatcgc attaaatac atgtttaag 
      tacaaaagt aattgtcta atgtataaa atagaataa 
      acgaagttt atggcacat tgagcgtag agatttggc 
      ttcattagg ctattgtaa ctgttggtc gtctcgaaa 
      aatatggat attatgaca taaatcaag agaaacgac 
      agttttaat gtgccacca tacttaaaa cttcccatt 
      ttgcaactt agaatactt aaatctact tacgtacat 
      ggccagtct acaagtcat tgttcattt gtctaacca 
      tgaaactct ttgtgggaa attattaat tgagtaaat 
      ttatttttt gagccgttg taacaaagt ttgaataat 
      ttggtttcc cgcgaatgc ttcatgagg cgcacgttt 
      caatttttg tttgaagat ttacaaaaa tatctcggc 
      aacgttttt gtaaatagc tataaatac tttagtatc 
      agtatttga ggatatatt cgtgtgtta tatattttt 
      ttgtatatc taccaaaag actgttatt ttattaatt 
      ttaaatata ttattagga taaaacagg ttagactat 
      ttattaagt gattttagt gtatttatg aaatcaatt 
      attgtgtag caatattag ccgtaagtg tagattata 
      tgaaaatta ctgtagttc caaaagtgt agtcctagt 
      tcataaatg acatccgat catccctac aaactttgt 
      gaaaatttg ttaattttt ttgatctga ttatctcag 
      ggatatttg atggtaatg tgcaaaaca tattcctgc 
      tttcattgt gttctagtt tttcgttca aagtttgta 
      agtatttaa ggcgaaagg ccagcattc taattcaat 
      gtattactg taaatacca acctatcat taaacatac 
      tcgtaaaag tttttgttt tattacttc ttcagctaa 
      ataccttgt ccaaaaaat agctcaaca tcattagca 
      ttttccaaa attaaaaca atatatctc attttagtt 
      g
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