| General information | |||
| TC033868
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 14894384..14981544 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033868 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04537 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
| Comment: last club segment elongated |
- >TC033868 RA
MEMDPQERE SDLGDDSPV SHNDEEISD GDGESGREH DDVDSCDEI RRHGGGILA DHGDVLAKL KMQVRDIKD DFENATRHL NFPLTNRSL QDNFHLPAP FPFPHPSTP FMTPLLPPP AAPSSGSSH SSEGSASSQ HTWSFEEQF KQVRQWHRR LISFLFQLY EINDDPKRK EFLDDLFSF MQKRGTPIN RLPIMAKSV LDLYELYNL VIARGGLVD VINKKLWQE IIKGLHLPS SITSAAFTL RTQYMKYLY PYECEKRRL STPAELQAA IDGNRREGR RSSYGQYDS MQRSPNPSQ MSPLSLVSQ QQQLAARMN SVMSGGMAL HNGAHHPSH PQPLGQPLN GGPLPGLAP SEFEARMME YVKLLNKEI RSSAATPPR QGDVSPPNA TSPLNQLEL SRITLWNMY NNNQPPVEP QKEALNLSD PTQPSVKRE PEHRDSPPP PKKFSKDDD EQKISPATH IKINSRGES RDARNGDSS LVVSMELNG ITYQGVLFA QSSPSSGRT AMAS
- >TC033868 RB
MEMDPQERE SDLGDDSPV SHNDEEISD GDGESGREH DDVDSCDEI RRHGGGILA DHGDVLAKL KMQVRDIKV GDDFENATR HLNFPLTNR SLQDNFHLP APFPFPHPS TPFMTPLLP PPAAPSSGS SHSSEGSAS SQHTWSFEE QFKQVRQWH RRLISFLFQ LYEINDDPK RKEFLDDLF SFMQKRGTP INRLPIMAK SVLDLYELY NLVIARGGL VDVINKKLW QEIIKGLHL PSSITSAAF TLRTQYMKY LYPYECEKR RLSTPAELQ AAIDGNRRE GRRSSYGQY DSMQRSPNP SQMSPLSLV SQQQQLAAR MNSVMSGGM ALHNGAHHP SHPQPLGQP LNGGPLPGL APSEFEARM MEYVKLLNK EIRSSAATP PRQGDVSPP NATSPLNQL ELSRITLWN MYNNNQPPV EPQKEALNL SDPTQPSVK REPEHRDSP PPPKKFSKD DDEQKISPA THIKINSRG ESRDARNGD SSLVVSMEL NGITYQGVL FAQSSPSSG RTAMAS
- >TC033868 RA
atggaaatg gatccgcaa gaacgggaa agtgatctg ggggatgat agtccggtg tcccacaat gacgaagaa ataagtgac ggtgacgga gagtcgggc cgcgaacat gacgatgtt gatagctgt gatgagata aggaggcac gggggcgga attctggca gatcacgga gatgttctt gcaaagttg aaaatgcaa gttagggac atcaaggac gacttcgaa aacgccacc cgccacctg aatttcccc ctcacaaat cgaagcctg caggataat ttccatttg ccggctccg tttccattt ccacacccg agtacaccg ttcatgaca cctctttta ccaccacca gcagctcca tcttcgggg agttcacac tcgtcggaa ggtagtgca tcctcacag cacacctgg tcgttcgaa gaacaattc aaacaggtg cgtcagtgg catcgccga ttaattagt tttttgttt cagttgtat gaaatcaac gacgacccc aaacggaaa gaattcctc gacgatctc ttctccttc atgcagaaa cggggtact ccgatcaat cgactgccc atcatggcc aaatccgtt ctcgattta tacgaattg tacaattta gtcatcgcg aggggtgga cttgttgat gtcatcaat aagaaactc tggcaggaa attatcaaa gggctgcat ctaccgtcg tcgataacg tcggcagct ttcacactc aggacgcaa tacatgaaa tacctgtac ccttacgaa tgcgagaaa cgccgcctc agcacaccg gccgagctc caggcggcc atcgacggc aaccgtcgc gaaggccgc cgcagcagc tacggccag tacgactcc atgcagcgc tcccccaac ccttcgcag atgtctcct ttgtccctc gtctcccag cagcaacaa ctcgccgcc cgcatgaac tccgtcatg agcggtggc atggccctc cacaacggc gcccaccac cccagccac ccccagccc ttgggtcag ccgctcaac ggcggcccg ctccctggc ctcgccccc agcgagttc gaggcgcgc atgatggaa tacgtcaag ctcctcaac aaggaaatc cgcagctcg gcggctact ccgccacga caaggggac gtgtcgccc cctaacgcc acctcgcca ctcaaccag ctggagctg tcgcggata acgctctgg aatatgtac aataataat caacccccc gtcgagccg caaaaggaa gcgttgaat ctctccgac ccgacgcag ccttcggtg aaacgggag ccggagcat agagattcg ccaccgccc ccgaagaag ttttccaaa gatgatgat gaacagaag atctcgccc gccacgcat atcaagatt aacagtaga ggtgagtct agggatgcg cgtaacgga gacagttcc ttagtagtc agtatggaa ttaaatgga attacatat caaggggtc ctattcgcg cagtcttcg ccatctagc ggccgaacg gcgatggcc tcgtaa
- >TC033868 RB
atggaaatg gatccgcaa gaacgggaa agtgatctg ggggatgat agtccggtg tcccacaat gacgaagaa ataagtgac ggtgacgga gagtcgggc cgcgaacat gacgatgtt gatagctgt gatgagata aggaggcac gggggcgga attctggca gatcacgga gatgttctt gcaaagttg aaaatgcaa gttagggac atcaaggta ggggacgac ttcgaaaac gccacccgc cacctgaat ttccccctc acaaatcga agcctgcag gataatttc catttgccg gctccgttt ccatttcca cacccgagt acaccgttc atgacacct cttttacca ccaccagca gctccatct tcggggagt tcacactcg tcggaaggt agtgcatcc tcacagcac acctggtcg ttcgaagaa caattcaaa caggtgcgt cagtggcat cgccgatta attagtttt ttgtttcag ttgtatgaa atcaacgac gaccccaaa cggaaagaa ttcctcgac gatctcttc tccttcatg cagaaacgg ggtactccg atcaatcga ctgcccatc atggccaaa tccgttctc gatttatac gaattgtac aatttagtc atcgcgagg ggtggactt gttgatgtc atcaataag aaactctgg caggaaatt atcaaaggg ctgcatcta ccgtcgtcg ataacgtcg gcagctttc acactcagg acgcaatac atgaaatac ctgtaccct tacgaatgc gagaaacgc cgcctcagc acaccggcc gagctccag gcggccatc gacggcaac cgtcgcgaa ggccgccgc agcagctac ggccagtac gactccatg cagcgctcc cccaaccct tcgcagatg tctcctttg tccctcgtc tcccagcag caacaactc gccgcccgc atgaactcc gtcatgagc ggtggcatg gccctccac aacggcgcc caccacccc agccacccc cagcccttg ggtcagccg ctcaacggc ggcccgctc cctggcctc gcccccagc gagttcgag gcgcgcatg atggaatac gtcaagctc ctcaacaag gaaatccgc agctcggcg gctactccg ccacgacaa ggggacgtg tcgccccct aacgccacc tcgccactc aaccagctg gagctgtcg cggataacg ctctggaat atgtacaat aataatcaa ccccccgtc gagccgcaa aaggaagcg ttgaatctc tccgacccg acgcagcct tcggtgaaa cgggagccg gagcataga gattcgcca ccgcccccg aagaagttt tccaaagat gatgatgaa cagaagatc tcgcccgcc acgcatatc aagattaac agtagaggt gagtctagg gatgcgcgt aacggagac agttcctta gtagtcagt atggaatta aatggaatt acatatcaa ggggtccta ttcgcgcag tcttcgcca tctagcggc cgaacggcg atggcctcg taa
- >TC033868 RA
ttggagatg tgcgctagc gtttgttgg tagtgtttc tcgcctagc acacttgac cagaattga acgctgaaa gcacatgac ataaccaaa cttttcgcg ggtgtttac gtgagtgtg tttcggttt acgtttttt ttcggtgtg gtgtgcaga gtgctttga gcattctgc aaaagacaa tttagcaaa attcgcgtc aaaaacaga gaacccgtt cccgtttat gccatggaa atggatccg caagaacgg gaaagtgat ctgggggat gatagtccg gtgtcccac aatgacgaa gaaataagt gacggtgac ggagagtcg ggccgcgaa catgacgat gttgatagc tgtgatgag ataaggagg cacgggggc ggaattctg gcagatcac ggagatgtt cttgcaaag ttgaaaatg caagttagg gacatcaag gacgacttc gaaaacgcc acccgccac ctgaatttc cccctcaca aatcgaagc ctgcaggat aatttccat ttgccggct ccgtttcca tttccacac ccgagtaca ccgttcatg acacctctt ttaccacca ccagcagct ccatcttcg gggagttca cactcgtcg gaaggtagt gcatcctca cagcacacc tggtcgttc gaagaacaa ttcaaacag gtgcgtcag tggcatcgc cgattaatt agttttttg tttcagttg tatgaaatc aacgacgac cccaaacgg aaagaattc ctcgacgat ctcttctcc ttcatgcag aaacggggt actccgatc aatcgactg cccatcatg gccaaatcc gttctcgat ttatacgaa ttgtacaat ttagtcatc gcgaggggt ggacttgtt gatgtcatc aataagaaa ctctggcag gaaattatc aaagggctg catctaccg tcgtcgata acgtcggca gctttcaca ctcaggacg caatacatg aaatacctg tacccttac gaatgcgag aaacgccgc ctcagcaca ccggccgag ctccaggcg gccatcgac ggcaaccgt cgcgaaggc cgccgcagc agctacggc cagtacgac tccatgcag cgctccccc aacccttcg cagatgtct cctttgtcc ctcgtctcc cagcagcaa caactcgcc gcccgcatg aactccgtc atgagcggt ggcatggcc ctccacaac ggcgcccac caccccagc cacccccag cccttgggt cagccgctc aacggcggc ccgctccct ggcctcgcc cccagcgag ttcgaggcg cgcatgatg gaatacgtc aagctcctc aacaaggaa atccgcagc tcggcggct actccgcca cgacaaggg gacgtgtcg ccccctaac gccacctcg ccactcaac cagctggag ctgtcgcgg ataacgctc tggaatatg tacaataat aatcaaccc cccgtcgag ccgcaaaag gaagcgttg aatctctcc gacccgacg cagccttcg gtgaaacgg gagccggag catagagat tcgccaccg cccccgaag aagttttcc aaagatgat gatgaacag aagatctcg cccgccacg catatcaag attaacagt agaggtgag tctagggat gcgcgtaac ggagacagt tccttagta gtcagtatg gaattaaat ggaattaca tatcaaggg gtcctattc gcgcagtct tcgccatct agcggccga acggcgatg gcctcgtaa agggaacct ctctgaatg tgatgtaga accctaatt tggcggtct gtgtggcgc caaattgga cacgctttt caattgttt tatatttat tatttttct agttgagtg tttttagtg atctagtgc tccaggaac tatgtgcac tttttataa tatggatgt ttttttttt gtaaagctg taaaatccg taatggcat cgcattaaa tacatgttt aagtacaaa agtaattgt ctaatgtat aaaatagaa taaacgaag tttatggca cattgagcg tagagattt ggcttcatt aggctattg taactgttg gtcgtctcg aaaaatatg gatattatg acataaatc aagagaaac gacagtttt aatgtgcca ccatactta aaacttccc attttgcaa cttagaata cttaaatct acttacgta catggccag tctacaagt cattgttca tttgtctaa ccatgaaac tctttgtgg gaaattatt aattgagta aatttattt tttgagccg ttgtaacaa agtttgaat aatttggtt tcccgcgaa tgcttcatg aggcgcacg tttcaattt ttgtttgaa gatttacaa aaatatctc ggcaacgtt tttgtaaat agctataaa tactttagt atcagtatt tgaggatat attcgtgtg ttatatatt tttttgtat atctaccaa aagactgtt attttatta attttaaat atattatta ggataaaac aggttagac tatttatta agtgatttt agtgtattt atgaaatca attattgtg tagcaatat tagccgtaa gtgtagatt atatgaaaa ttactgtag ttccaaaag tgtagtcct agttcataa atgacatcc gatcatccc tacaaactt tgtgaaaat ttgttaatt tttttgatc tgattatct cagggatat ttgatggta atgtgcaaa acatattcc tgctttcat tgtgttcta gtttttcgt tcaaagttt gtaagtatt taaggcgaa aggccagca ttctaattc aatgtatta ctgtaaata ccaacctat cattaaaca tactcgtaa aagtttttg ttttattac ttcttcagc taaatacct tgtccaaaa aatagctca acatcatta gcattttcc aaaattaaa acaatatat ctcatttta gttg
- >TC033868 RB
ttggagatg tgcgctagc gtttgttgg tagtgtttc tcgcctagc acacttgac cagaattga acgctgaaa gcacatgac ataaccaaa cttttcgcg ggtgtttac gtgagtgtg tttcggttt acgtttttt ttcggtgtg gtgtgcaga gtgctttga gcattctgc aaaagacaa tttagcaaa attcgcgtc aaaaacaga gaacccgtt cccgtttat gccatggaa atggatccg caagaacgg gaaagtgat ctgggggat gatagtccg gtgtcccac aatgacgaa gaaataagt gacggtgac ggagagtcg ggccgcgaa catgacgat gttgatagc tgtgatgag ataaggagg cacgggggc ggaattctg gcagatcac ggagatgtt cttgcaaag ttgaaaatg caagttagg gacatcaag gtaggggac gacttcgaa aacgccacc cgccacctg aatttcccc ctcacaaat cgaagcctg caggataat ttccatttg ccggctccg tttccattt ccacacccg agtacaccg ttcatgaca cctctttta ccaccacca gcagctcca tcttcgggg agttcacac tcgtcggaa ggtagtgca tcctcacag cacacctgg tcgttcgaa gaacaattc aaacaggtg cgtcagtgg catcgccga ttaattagt tttttgttt cagttgtat gaaatcaac gacgacccc aaacggaaa gaattcctc gacgatctc ttctccttc atgcagaaa cggggtact ccgatcaat cgactgccc atcatggcc aaatccgtt ctcgattta tacgaattg tacaattta gtcatcgcg aggggtgga cttgttgat gtcatcaat aagaaactc tggcaggaa attatcaaa gggctgcat ctaccgtcg tcgataacg tcggcagct ttcacactc aggacgcaa tacatgaaa tacctgtac ccttacgaa tgcgagaaa cgccgcctc agcacaccg gccgagctc caggcggcc atcgacggc aaccgtcgc gaaggccgc cgcagcagc tacggccag tacgactcc atgcagcgc tcccccaac ccttcgcag atgtctcct ttgtccctc gtctcccag cagcaacaa ctcgccgcc cgcatgaac tccgtcatg agcggtggc atggccctc cacaacggc gcccaccac cccagccac ccccagccc ttgggtcag ccgctcaac ggcggcccg ctccctggc ctcgccccc agcgagttc gaggcgcgc atgatggaa tacgtcaag ctcctcaac aaggaaatc cgcagctcg gcggctact ccgccacga caaggggac gtgtcgccc cctaacgcc acctcgcca ctcaaccag ctggagctg tcgcggata acgctctgg aatatgtac aataataat caacccccc gtcgagccg caaaaggaa gcgttgaat ctctccgac ccgacgcag ccttcggtg aaacgggag ccggagcat agagattcg ccaccgccc ccgaagaag ttttccaaa gatgatgat gaacagaag atctcgccc gccacgcat atcaagatt aacagtaga ggtgagtct agggatgcg cgtaacgga gacagttcc ttagtagtc agtatggaa ttaaatgga attacatat caaggggtc ctattcgcg cagtcttcg ccatctagc ggccgaacg gcgatggcc tcgtaaagg gaacctctc tgaatgtga tgtagaacc ctaatttgg cggtctgtg tggcgccaa attggacac gcttttcaa ttgttttat atttattat ttttctagt tgagtgttt ttagtgatc tagtgctcc aggaactat gtgcacttt ttataatat ggatgtttt ttttttgta aagctgtaa aatccgtaa tggcatcgc attaaatac atgtttaag tacaaaagt aattgtcta atgtataaa atagaataa acgaagttt atggcacat tgagcgtag agatttggc ttcattagg ctattgtaa ctgttggtc gtctcgaaa aatatggat attatgaca taaatcaag agaaacgac agttttaat gtgccacca tacttaaaa cttcccatt ttgcaactt agaatactt aaatctact tacgtacat ggccagtct acaagtcat tgttcattt gtctaacca tgaaactct ttgtgggaa attattaat tgagtaaat ttatttttt gagccgttg taacaaagt ttgaataat ttggtttcc cgcgaatgc ttcatgagg cgcacgttt caatttttg tttgaagat ttacaaaaa tatctcggc aacgttttt gtaaatagc tataaatac tttagtatc agtatttga ggatatatt cgtgtgtta tatattttt ttgtatatc taccaaaag actgttatt ttattaatt ttaaatata ttattagga taaaacagg ttagactat ttattaagt gattttagt gtatttatg aaatcaatt attgtgtag caatattag ccgtaagtg tagattata tgaaaatta ctgtagttc caaaagtgt agtcctagt tcataaatg acatccgat catccctac aaactttgt gaaaatttg ttaattttt ttgatctga ttatctcag ggatatttg atggtaatg tgcaaaaca tattcctgc tttcattgt gttctagtt tttcgttca aagtttgta agtatttaa ggcgaaagg ccagcattc taattcaat gtattactg taaatacca acctatcat taaacatac tcgtaaaag tttttgttt tattacttc ttcagctaa ataccttgt ccaaaaaat agctcaaca tcattagca ttttccaaa attaaaaca atatatctc attttagtt g
