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Gene information
General information
TC008701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14352000..14356821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008701
      iBeetle screen iB_04535

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larva segment random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008701 RA
        MDSDFEDKC QDIALKRQV RKASVQKYL PAYIQTPED 
        FNLVAKSEE PADKNTEET EQTNPSQEV KVPEKPKTE 
        EKLNESTSS TTKLNTSKK RRSQNSWQR GYVKKRKRT 
        SKTSECLQQ DCEKKDSSD DSKDVNVTS EPEDVPAET 
        VESTAPLTI NCNESPQKE NAELQSPRR RLSDLMSPS 
        ELLENPLEF DDIDQALNE NVEEPVNRP PVECSQKEL 
        EKILEQTVQ ITSGCSLQP LLDLYNQLS RIVKKFSNT 
        HLRTDLPKE LGVELNRFK NEASLETVS ERSPSSDQS 
        T
      CDS sequence
      • >TC008701 RA
        atggacagc gatttcgaa gataagtgt caagacatc 
        gccttaaaa agacaagtc agaaaagcc agcgttcag 
        aaataccta ccagcatac attcaaacc ccagaggat 
        tttaatctc gttgcaaaa tcggaagaa ccagccgat 
        aaaaacact gaagaaact gagcaaaca aatccaagt 
        caagaagtc aaagtaccc gagaaacca aagaccgaa 
        gaaaaactt aatgagagt acgagtagt acgactaag 
        cttaacact tcgaaaaaa cggaggtct caaaacagt 
        tggcaaagg ggctatgtg aagaaaagg aagcggacg 
        agtaaaacg tcagaatgt ttgcagcaa gattgtgaa 
        aaaaaagat tcatcggat gattctaag gatgttaat 
        gttacgtct gaacctgaa gatgtgccc gctgaaact 
        gttgaaagt acggcgccg ttgaccatt aattgtaat 
        gaatcgccc cagaaagag aacgcagaa ttgcagtcg 
        cctaggagg aggttgagt gatctcatg agtccgtct 
        gaattatta gaaaatcct ttagaattt gatgatatc 
        gaccaagcc ctcaatgaa aatgttgaa gaaccagtg 
        aataggcca ccggttgag tgctcgcaa aaggagctg 
        gagaagatt ttggagcag acggtgcaa attacaagc 
        gggtgttcg ttgcagccg cttctagat ttatataat 
        cagctcagt aggatagtg aagaaattc tccaacact 
        catcttcga accgactta ccaaaggaa ttaggcgtt 
        gagttgaat aggttcaag aatgaagcc agtttggag 
        acagttagt gagaggagt ccttcgtcg gatcaatcc 
        acgtaa
      mRNA sequence
      • >TC008701 RA
        gcactttag caagaaaca taacattgt ctccgaacg 
        tatattcat gtgcatacg agacgtcag aagtaaaat 
        aaacacctt tcccaaaga ctgttcctt tagaagtcc 
        cacttcttc gccagtcaa aattaaatt tgcagatgc 
        tatcaacaa aagcattcc ttgttccat gaaataaag 
        ttttctcaa ctaaatggc cacatttgt cccacattt 
        gcttctttt ttaataaat aacaaccaa ccaaaacat 
        cggcagaca agcaaataa gacatagag cttgctcgc 
        ttcgcgatt acgcctaca ctttaatca aaaatgaga 
        tggacagcg atttcgaag ataagtgtc aagacatcg 
        ccttaaaaa gacaagtca gaaaagcca gcgttcaga 
        aatacctac cagcataca ttcaaaccc cagaggatt 
        ttaatctcg ttgcaaaat cggaagaac cagccgata 
        aaaacactg aagaaactg agcaaacaa atccaagtc 
        aagaagtca aagtacccg agaaaccaa agaccgaag 
        aaaaactta atgagagta cgagtagta cgactaagc 
        ttaacactt cgaaaaaac ggaggtctc aaaacagtt 
        ggcaaaggg gctatgtga agaaaagga agcggacga 
        gtaaaacgt cagaatgtt tgcagcaag attgtgaaa 
        aaaaagatt catcggatg attctaagg atgttaatg 
        ttacgtctg aacctgaag atgtgcccg ctgaaactg 
        ttgaaagta cggcgccgt tgaccatta attgtaatg 
        aatcgcccc agaaagaga acgcagaat tgcagtcgc 
        ctaggagga ggttgagtg atctcatga gtccgtctg 
        aattattag aaaatcctt tagaatttg atgatatcg 
        accaagccc tcaatgaaa atgttgaag aaccagtga 
        ataggccac cggttgagt gctcgcaaa aggagctgg 
        agaagattt tggagcaga cggtgcaaa ttacaagcg 
        ggtgttcgt tgcagccgc ttctagatt tatataatc 
        agctcagta ggatagtga agaaattct ccaacactc 
        atcttcgaa ccgacttac caaaggaat taggcgttg 
        agttgaata ggttcaaga atgaagcca gtttggaga 
        cagttagtg agaggagtc cttcgtcgg atcaatcca 
        cgtaacgta acatttatt attatatca tgagcaaca 
        ctgccacgg atttttaaa taagtttta aattattta 
        ccagtattt ttactttgt tctgtaata agccaacgg 
        tcttattta atggcgtgg ttacttatt aagttggtc 
        cctacaacc aaagaggag atggtataa ataattgca 
        ctgccatgg ggtaaggct gaataattt gttatttaa 
        taaggacta cttaatgtc agcgtatgt gcatgtgcc 
        ttcttaagg tgaagtgtc cacgaaacg atttcgcgt 
        ttaatgttt tcttcccat gtggtatct tcttagatt 
        gtgatttat attagtagg cagttgatg agaaaaaat 
        gcaatgtcg tacaagaac acgtgggaa gcggttgtt 
        ttagatgcc caatttttt aaacttact gtttaatgt 
        atagaaaat attttatta ttcagtttt ctactttat 
        aaggtactc acgtctttt tgaattaac gatttattt 
        tttactcca gaaattttg gatgaaagt gagtgaaat 
        tatgaagtt ttaaagtaa atgtgtttt gaaaatcaa 
        tctgttacc gaaaacttt tcatttttc ttgaattaa 
        ctttttgtt tgtctcagg catttttca aaaatgcta 
        aattccgta ataatattt tttttatta gccaatgtc 
        ttaattgtt tatctgcat taaaattaa actttgttt 
        ataataaat tagtaaaaa aaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY